Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity

Detalhes bibliográficos
Autor(a) principal: Esposito, Mariana Araújo
Data de Publicação: 2021
Outros Autores: Cesar, Pedro Henrique Souza, Brandão, Rafaela Magalhães, Abreu, Tatiane Silva de, Marcussi, Silvana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/48806
Resumo: The genus Eucalyptus present high content of essential oil (EO). This study evaluated the pharmacological properties of Eucalyptus grandis (EG) and Eucalyptus microcorys (EM) EOs. The major component in both EOs was 1,8-Cineole. Both essential oils prevented thrombus dissolution and reduced clotting, hemolysis, and genotoxicity induced by snake venoms. 50% (EM) and 73% (EG) were the greatest inhibitions obtained in the thrombolytic assay - thrombolysis induced by B. moojeni venom. Increases in clotting time were also observed, with values considered significant between 10-27 seconds. Lysis values 50% lower than the negative control were observed in both EOs. The EOs also protected fibrinogenolysis induced by snake venom. EM EO was more effective in reducing venom-induced DNA fragmentation in the comet assay, with arbitrary unit values 66.15% lower than the positive control. These oils present wide application potential considering the pharmacological properties observed in this study.
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spelling Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity1,8-cineoleSnake venom proteasesComet assayChemical characterizationEnzyme inhibitorsHemostasisThe genus Eucalyptus present high content of essential oil (EO). This study evaluated the pharmacological properties of Eucalyptus grandis (EG) and Eucalyptus microcorys (EM) EOs. The major component in both EOs was 1,8-Cineole. Both essential oils prevented thrombus dissolution and reduced clotting, hemolysis, and genotoxicity induced by snake venoms. 50% (EM) and 73% (EG) were the greatest inhibitions obtained in the thrombolytic assay - thrombolysis induced by B. moojeni venom. Increases in clotting time were also observed, with values considered significant between 10-27 seconds. Lysis values 50% lower than the negative control were observed in both EOs. The EOs also protected fibrinogenolysis induced by snake venom. EM EO was more effective in reducing venom-induced DNA fragmentation in the comet assay, with arbitrary unit values 66.15% lower than the positive control. These oils present wide application potential considering the pharmacological properties observed in this study.Wiley2022-01-07T15:32:34Z2022-01-07T15:32:34Z2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfESPOSITO, M. A. et al. Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity. Brazilian Archives of Biology and Technology, [S.l.], v. 64, p. 1-10, 2021. DOI: 10.1590/1678-4324-2021210109.http://repositorio.ufla.br/jspui/handle/1/48806Brazilian Archives of Biology and Technologyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessEsposito, Mariana AraújoCesar, Pedro Henrique SouzaBrandão, Rafaela MagalhãesAbreu, Tatiane Silva deMarcussi, Silvanaeng2022-01-07T15:32:34Zoai:localhost:1/48806Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2022-01-07T15:32:34Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity
title Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity
spellingShingle Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity
Esposito, Mariana Araújo
1,8-cineole
Snake venom proteases
Comet assay
Chemical characterization
Enzyme inhibitors
Hemostasis
title_short Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity
title_full Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity
title_fullStr Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity
title_full_unstemmed Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity
title_sort Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity
author Esposito, Mariana Araújo
author_facet Esposito, Mariana Araújo
Cesar, Pedro Henrique Souza
Brandão, Rafaela Magalhães
Abreu, Tatiane Silva de
Marcussi, Silvana
author_role author
author2 Cesar, Pedro Henrique Souza
Brandão, Rafaela Magalhães
Abreu, Tatiane Silva de
Marcussi, Silvana
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Esposito, Mariana Araújo
Cesar, Pedro Henrique Souza
Brandão, Rafaela Magalhães
Abreu, Tatiane Silva de
Marcussi, Silvana
dc.subject.por.fl_str_mv 1,8-cineole
Snake venom proteases
Comet assay
Chemical characterization
Enzyme inhibitors
Hemostasis
topic 1,8-cineole
Snake venom proteases
Comet assay
Chemical characterization
Enzyme inhibitors
Hemostasis
description The genus Eucalyptus present high content of essential oil (EO). This study evaluated the pharmacological properties of Eucalyptus grandis (EG) and Eucalyptus microcorys (EM) EOs. The major component in both EOs was 1,8-Cineole. Both essential oils prevented thrombus dissolution and reduced clotting, hemolysis, and genotoxicity induced by snake venoms. 50% (EM) and 73% (EG) were the greatest inhibitions obtained in the thrombolytic assay - thrombolysis induced by B. moojeni venom. Increases in clotting time were also observed, with values considered significant between 10-27 seconds. Lysis values 50% lower than the negative control were observed in both EOs. The EOs also protected fibrinogenolysis induced by snake venom. EM EO was more effective in reducing venom-induced DNA fragmentation in the comet assay, with arbitrary unit values 66.15% lower than the positive control. These oils present wide application potential considering the pharmacological properties observed in this study.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022-01-07T15:32:34Z
2022-01-07T15:32:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ESPOSITO, M. A. et al. Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity. Brazilian Archives of Biology and Technology, [S.l.], v. 64, p. 1-10, 2021. DOI: 10.1590/1678-4324-2021210109.
http://repositorio.ufla.br/jspui/handle/1/48806
identifier_str_mv ESPOSITO, M. A. et al. Eucalyptus grandis and Eucalyptus microcorys essential oils: in vitro modulation of hemostasis and genotoxic activity. Brazilian Archives of Biology and Technology, [S.l.], v. 64, p. 1-10, 2021. DOI: 10.1590/1678-4324-2021210109.
url http://repositorio.ufla.br/jspui/handle/1/48806
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1815438975118082048

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