Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study

Detalhes bibliográficos
Autor(a) principal: Pujatti,Priscilla Brunelli
Data de Publicação: 2005
Outros Autores: Simal,Carlos Jorge Rodrigues, Santos,Raquel Gouvêa dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700002
Resumo: Technetium-99m (99mTc) has been the radionuclide of choice for nuclear medicine procedures and experimental research. Because of its optimal nuclear properties, 99mTc is suitable for high efficiency detection with the advantage of reduced radiological waste. Crotalus venom (CV) has been shown to reduce tumors in clinical studies and tissue distribution studies are very important for clinical use. The goal of this work was to obtain CV labeled with 99mTc which preserves its biological activity. After labeling, biological activity was assessed by hemolytic activity evaluation. Labeled and crude venom caused indirect hemolysis provided that the incubation medium contained an exogenous source of lecithin. High yield radiolabeled-CV was obtained and biological activity was preserved. The results suggest that 99mTc-CV can be a useful tool for biodistribution studies.
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spelling Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution studyTechnetium-99mlabelinghemolytic activityTechnetium-99m (99mTc) has been the radionuclide of choice for nuclear medicine procedures and experimental research. Because of its optimal nuclear properties, 99mTc is suitable for high efficiency detection with the advantage of reduced radiological waste. Crotalus venom (CV) has been shown to reduce tumors in clinical studies and tissue distribution studies are very important for clinical use. The goal of this work was to obtain CV labeled with 99mTc which preserves its biological activity. After labeling, biological activity was assessed by hemolytic activity evaluation. Labeled and crude venom caused indirect hemolysis provided that the incubation medium contained an exogenous source of lecithin. High yield radiolabeled-CV was obtained and biological activity was preserved. The results suggest that 99mTc-CV can be a useful tool for biodistribution studies.Instituto de Tecnologia do Paraná - Tecpar2005-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700002Brazilian Archives of Biology and Technology v.48 n.spe2 2005reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132005000700002info:eu-repo/semantics/openAccessPujatti,Priscilla BrunelliSimal,Carlos Jorge RodriguesSantos,Raquel Gouvêa doseng2005-12-09T00:00:00Zoai:scielo:S1516-89132005000700002Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2005-12-09T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study
title Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study
spellingShingle Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study
Pujatti,Priscilla Brunelli
Technetium-99m
labeling
hemolytic activity
title_short Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study
title_full Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study
title_fullStr Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study
title_full_unstemmed Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study
title_sort Preparation of crotalus venom radiolabeled with technetium-99m as a tool for biodistribution study
author Pujatti,Priscilla Brunelli
author_facet Pujatti,Priscilla Brunelli
Simal,Carlos Jorge Rodrigues
Santos,Raquel Gouvêa dos
author_role author
author2 Simal,Carlos Jorge Rodrigues
Santos,Raquel Gouvêa dos
author2_role author
author
dc.contributor.author.fl_str_mv Pujatti,Priscilla Brunelli
Simal,Carlos Jorge Rodrigues
Santos,Raquel Gouvêa dos
dc.subject.por.fl_str_mv Technetium-99m
labeling
hemolytic activity
topic Technetium-99m
labeling
hemolytic activity
description Technetium-99m (99mTc) has been the radionuclide of choice for nuclear medicine procedures and experimental research. Because of its optimal nuclear properties, 99mTc is suitable for high efficiency detection with the advantage of reduced radiological waste. Crotalus venom (CV) has been shown to reduce tumors in clinical studies and tissue distribution studies are very important for clinical use. The goal of this work was to obtain CV labeled with 99mTc which preserves its biological activity. After labeling, biological activity was assessed by hemolytic activity evaluation. Labeled and crude venom caused indirect hemolysis provided that the incubation medium contained an exogenous source of lecithin. High yield radiolabeled-CV was obtained and biological activity was preserved. The results suggest that 99mTc-CV can be a useful tool for biodistribution studies.
publishDate 2005
dc.date.none.fl_str_mv 2005-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000700002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132005000700002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.48 n.spe2 2005
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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