Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity

Detalhes bibliográficos
Autor(a) principal: Santos,Neyliane Gonçalves dos
Data de Publicação: 2008
Outros Autores: Amaral,Ademir, Cavalcanti,Mariana Brayner, Neves,Maria Amélia Batista, Machado,Cíntia Gonsalves de Faria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700018
Resumo: The aim of this work was to establish a protocol to evaluate ionizing radiation effects on P-glycoprotein (P-gp) activity. For this, human peripheral blood samples were irradiated in vitro with different doses and P-gp activity was analyzed for CD4 and CD8 T lymphocytes through rhodamine123-efflux assay by flow cytometry. By simultaneous employment of percentage and mean fluorescence index parameters, subject-by-subject analysis pointed out changes in P-gp activity for some individuals and irradiated samples. Based on this work, the proposed protocol was considered adequate for evaluating P-gp activity on cells after radioactive stress. Besides, this research suggests that P-gp activity could be an important factor to define patient-specific protocols in combined chemo-and radiotherapy, particularly when radiation exposure precedes chemical treatment.
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spelling Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activitycombined therapyradiation effectP-glycoproteinmultidrug resistancefunctional analysisflow cytometryThe aim of this work was to establish a protocol to evaluate ionizing radiation effects on P-glycoprotein (P-gp) activity. For this, human peripheral blood samples were irradiated in vitro with different doses and P-gp activity was analyzed for CD4 and CD8 T lymphocytes through rhodamine123-efflux assay by flow cytometry. By simultaneous employment of percentage and mean fluorescence index parameters, subject-by-subject analysis pointed out changes in P-gp activity for some individuals and irradiated samples. Based on this work, the proposed protocol was considered adequate for evaluating P-gp activity on cells after radioactive stress. Besides, this research suggests that P-gp activity could be an important factor to define patient-specific protocols in combined chemo-and radiotherapy, particularly when radiation exposure precedes chemical treatment.Instituto de Tecnologia do Paraná - Tecpar2008-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700018Brazilian Archives of Biology and Technology v.51 n.spe 2008reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132008000700018info:eu-repo/semantics/openAccessSantos,Neyliane Gonçalves dosAmaral,AdemirCavalcanti,Mariana BraynerNeves,Maria Amélia BatistaMachado,Cíntia Gonsalves de Fariaeng2009-02-20T00:00:00Zoai:scielo:S1516-89132008000700018Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2009-02-20T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity
title Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity
spellingShingle Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity
Santos,Neyliane Gonçalves dos
combined therapy
radiation effect
P-glycoprotein
multidrug resistance
functional analysis
flow cytometry
title_short Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity
title_full Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity
title_fullStr Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity
title_full_unstemmed Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity
title_sort Flow cytometry protocol to evaluate ionizing radiation effects on P-glycoprotein activity
author Santos,Neyliane Gonçalves dos
author_facet Santos,Neyliane Gonçalves dos
Amaral,Ademir
Cavalcanti,Mariana Brayner
Neves,Maria Amélia Batista
Machado,Cíntia Gonsalves de Faria
author_role author
author2 Amaral,Ademir
Cavalcanti,Mariana Brayner
Neves,Maria Amélia Batista
Machado,Cíntia Gonsalves de Faria
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Santos,Neyliane Gonçalves dos
Amaral,Ademir
Cavalcanti,Mariana Brayner
Neves,Maria Amélia Batista
Machado,Cíntia Gonsalves de Faria
dc.subject.por.fl_str_mv combined therapy
radiation effect
P-glycoprotein
multidrug resistance
functional analysis
flow cytometry
topic combined therapy
radiation effect
P-glycoprotein
multidrug resistance
functional analysis
flow cytometry
description The aim of this work was to establish a protocol to evaluate ionizing radiation effects on P-glycoprotein (P-gp) activity. For this, human peripheral blood samples were irradiated in vitro with different doses and P-gp activity was analyzed for CD4 and CD8 T lymphocytes through rhodamine123-efflux assay by flow cytometry. By simultaneous employment of percentage and mean fluorescence index parameters, subject-by-subject analysis pointed out changes in P-gp activity for some individuals and irradiated samples. Based on this work, the proposed protocol was considered adequate for evaluating P-gp activity on cells after radioactive stress. Besides, this research suggests that P-gp activity could be an important factor to define patient-specific protocols in combined chemo-and radiotherapy, particularly when radiation exposure precedes chemical treatment.
publishDate 2008
dc.date.none.fl_str_mv 2008-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132008000700018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.51 n.spe 2008
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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