Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters

Detalhes bibliográficos
Autor(a) principal: Oliveira, Fernanda Schoma de
Data de Publicação: 2011
Outros Autores: Falbo, Margarete Kimie, Sandini, Itacir Eloi, Ishiy, Larissa Emy
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4795
Resumo: This study aimed to evaluate the effect of freezing (- 20 ºC) and time of storage of serum in the determination of biochemical values in sheep. It was held at the Universidade Estadual do Centro-Oeste in Guarapuava – PR. The serum was obtained from 32 Corriedale x Ille de France crossbred lambs, four months old. Nine hundred and sixty tests were performed, the first immediately after sampling (time zero), after 7, 14, 21, 28 days after blood collection. The analytes tested were aspartate aminotranferase (AST), urea, total protein, cholesterol, phosphorus and magnesium. The results showed that the time of storage of frozen samples (-20 ºC) was able to alter results of total protein, phosphorus, and magnesium. The serum measurements of AST, cholesterol and urea did not differ in different storage times.
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spelling Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parametersEfeito do congelamento e do tempo de armazenamento do soro sanguíneo de cordeiros na determinação de parâmetros bioquímicosSerum stabilitySheepsTime of storage.Estabilidade do soroCordeirosTempo de armazenamento.This study aimed to evaluate the effect of freezing (- 20 ºC) and time of storage of serum in the determination of biochemical values in sheep. It was held at the Universidade Estadual do Centro-Oeste in Guarapuava – PR. The serum was obtained from 32 Corriedale x Ille de France crossbred lambs, four months old. Nine hundred and sixty tests were performed, the first immediately after sampling (time zero), after 7, 14, 21, 28 days after blood collection. The analytes tested were aspartate aminotranferase (AST), urea, total protein, cholesterol, phosphorus and magnesium. The results showed that the time of storage of frozen samples (-20 ºC) was able to alter results of total protein, phosphorus, and magnesium. The serum measurements of AST, cholesterol and urea did not differ in different storage times.Este trabalho teve como objetivo avaliar o efeito do congelamento (- 20 ºC) e o tempo de armazenamento do soro na determinação de valores bioquímicos em ovinos. Foi realizado na Universidade Estadual do Centro-Oeste em Guarapuava – PR. O soro foi obtido de 32 cordeiros da raça Ile de France x Corriedale, com 4 meses de idade. Foram realizadas análises, imediatamente após a obtenção da amostra, tempo zero e após 7, 14, 21, 28 dias da colheita do sangue. Os analitos testados foram aspartato-amino-transferase (AST), uréia, proteína total, colesterol, fósforo e magnésio. Os resultados permitiram concluir que o tempo de armazenamento das amostras congeladas (- 20 ºC) causou alterações nos resultados para as análises de proteína total, fósforo, e magnésio. As determinações séricas AST, colesterol e uréia não diferiram estatisticamente nos diferentes tempos de armazenamento. UEL2011-07-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpesquisa empírica de campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/479510.5433/1679-0359.2011v32n2p717Semina: Ciências Agrárias; Vol. 32 No. 2 (2011); 717-722Semina: Ciências Agrárias; v. 32 n. 2 (2011); 717-7221679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4795/8430Oliveira, Fernanda Schoma deFalbo, Margarete KimieSandini, Itacir EloiIshiy, Larissa Emyinfo:eu-repo/semantics/openAccess2016-03-09T17:00:22Zoai:ojs.pkp.sfu.ca:article/4795Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2016-03-09T17:00:22Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters
Efeito do congelamento e do tempo de armazenamento do soro sanguíneo de cordeiros na determinação de parâmetros bioquímicos
title Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters
spellingShingle Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters
Oliveira, Fernanda Schoma de
Serum stability
Sheeps
Time of storage.
Estabilidade do soro
Cordeiros
Tempo de armazenamento.
title_short Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters
title_full Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters
title_fullStr Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters
title_full_unstemmed Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters
title_sort Effect of freezing and storage time of blood serum of lambs in the determination of biochemical parameters
author Oliveira, Fernanda Schoma de
author_facet Oliveira, Fernanda Schoma de
Falbo, Margarete Kimie
Sandini, Itacir Eloi
Ishiy, Larissa Emy
author_role author
author2 Falbo, Margarete Kimie
Sandini, Itacir Eloi
Ishiy, Larissa Emy
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira, Fernanda Schoma de
Falbo, Margarete Kimie
Sandini, Itacir Eloi
Ishiy, Larissa Emy
dc.subject.por.fl_str_mv Serum stability
Sheeps
Time of storage.
Estabilidade do soro
Cordeiros
Tempo de armazenamento.
topic Serum stability
Sheeps
Time of storage.
Estabilidade do soro
Cordeiros
Tempo de armazenamento.
description This study aimed to evaluate the effect of freezing (- 20 ºC) and time of storage of serum in the determination of biochemical values in sheep. It was held at the Universidade Estadual do Centro-Oeste in Guarapuava – PR. The serum was obtained from 32 Corriedale x Ille de France crossbred lambs, four months old. Nine hundred and sixty tests were performed, the first immediately after sampling (time zero), after 7, 14, 21, 28 days after blood collection. The analytes tested were aspartate aminotranferase (AST), urea, total protein, cholesterol, phosphorus and magnesium. The results showed that the time of storage of frozen samples (-20 ºC) was able to alter results of total protein, phosphorus, and magnesium. The serum measurements of AST, cholesterol and urea did not differ in different storage times.
publishDate 2011
dc.date.none.fl_str_mv 2011-07-12
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
pesquisa empírica de campo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4795
10.5433/1679-0359.2011v32n2p717
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4795
identifier_str_mv 10.5433/1679-0359.2011v32n2p717
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4795/8430
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 32 No. 2 (2011); 717-722
Semina: Ciências Agrárias; v. 32 n. 2 (2011); 717-722
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306061209927680

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