Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6056 |
Resumo: | A great number of pharmacological compounds is found in the Brazilian marine diversity. This study evaluated the anticoagulant potential of glycosaminoglycans (GAGs) isolated from the skin of ‘palombeta’ Chloroscombrus chrysurus and ‘guaiúba’ Ocyurus chrysurus. GAGs were extracted with crude papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by ion exchange chromatography on DEAE-cellulose column. The chemical composition (contaminant proteins and total carbohydrates) and the analysis by 0.5% agarose gel electrophoresis of fractions were also determined. Anticoagulant assays were performed by activated partial thromboplastin time (APTT) using normal human plasma and standard heparin (193.00 IU mg-1). The obtaining and fractionation procedures of GAGs were effective and similar chromatographic profiles were verified between the species. A similar mobility to dermatan sulfate was revealed for C. chrysurus. This GAG also showed a low activity of 3.30 IU mg-1. |
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Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056Glicosaminoglicanos isolados da pele de palombeta (Chloroscombrus chrysurus) e guaiúba (Ocyurus chrysurus): características e implicações biológicas - doi: 10.4025/actascibiolsci.v34i2.6056marine teleostssulfated glycosaminoglycansblood coagulationAPTT testteleósteos marinhosglicosaminoglicanos sulfatadoscoagulação sanguíneateste do TTPAQuímica de macromoléculasA great number of pharmacological compounds is found in the Brazilian marine diversity. This study evaluated the anticoagulant potential of glycosaminoglycans (GAGs) isolated from the skin of ‘palombeta’ Chloroscombrus chrysurus and ‘guaiúba’ Ocyurus chrysurus. GAGs were extracted with crude papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by ion exchange chromatography on DEAE-cellulose column. The chemical composition (contaminant proteins and total carbohydrates) and the analysis by 0.5% agarose gel electrophoresis of fractions were also determined. Anticoagulant assays were performed by activated partial thromboplastin time (APTT) using normal human plasma and standard heparin (193.00 IU mg-1). The obtaining and fractionation procedures of GAGs were effective and similar chromatographic profiles were verified between the species. A similar mobility to dermatan sulfate was revealed for C. chrysurus. This GAG also showed a low activity of 3.30 IU mg-1.O Brasil abriga uma das maiores biodiversidades marinhas do mundo, favorecendo a descoberta de fontes alternativas de compostos farmacológicos. Desta forma, objetivou-se avaliar o potencial anticoagulante de glicosaminoglicanos (GAGs) isolados das peles da palombeta (Chloroscombrus chrysurus) e guaiúba (Ocyurus chrysurus). Os GAGs foram extraídos com papaína bruta em tampão acetato de sódio 0,1 M (pH 5,0) contendo cisteína 5 mM e EDTA 5 mM, seguido por cromatografia de troca iônica do extrato total em coluna de DEAE-celulose. As frações obtidas foram analisadas quanto à composição química (proteínas contaminantes e carboidratos totais) e os GAGs identificados por eletroforese em gel de agarose a 0,5%. Os ensaios de atividade anticoagulante foram realizados por meio do tempo de tromboplastina parcial ativada (TTPA) usando plasma humano normal e heparina-padrão (193,00 UI mg-1). O procedimento de obtenção e fracionamento dos GAGs mostrou-se eficiente, indicando semelhantes perfis cromatográficos entre as espécies avaliadas e, revelando para C. chrysurus, bandas com mobilidades semelhantes ao dermatam sulfato e com atividade de apenas 3,30 UI mg-1.Universidade Estadual De Maringá2011-10-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionBioprospecção ; composição bioquímica ; relações biológicasapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/605610.4025/actascibiolsci.v34i2.6056Acta Scientiarum. Biological Sciences; Vol 34 No 2 (2012); 141-148Acta Scientiarum. Biological Sciences; v. 34 n. 2 (2012); 141-1481807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMporhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6056/pdfRodrigues, José Ariévilo GurgelVanderlei, Edfranck de Sousa OliveiraQuinderé, Ana Luíza GomesFontes, Bruno PedrosaQueiroz, Ismael Nilo Lino deBenevides, Norma Maria Barrosinfo:eu-repo/semantics/openAccess2022-11-23T17:33:51Zoai:periodicos.uem.br/ojs:article/6056Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-11-23T17:33:51Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056 Glicosaminoglicanos isolados da pele de palombeta (Chloroscombrus chrysurus) e guaiúba (Ocyurus chrysurus): características e implicações biológicas - doi: 10.4025/actascibiolsci.v34i2.6056 |
title |
Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056 |
spellingShingle |
Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056 Rodrigues, José Ariévilo Gurgel marine teleosts sulfated glycosaminoglycans blood coagulation APTT test teleósteos marinhos glicosaminoglicanos sulfatados coagulação sanguínea teste do TTPA Química de macromoléculas |
title_short |
Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056 |
title_full |
Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056 |
title_fullStr |
Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056 |
title_full_unstemmed |
Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056 |
title_sort |
Glycosaminoglycans isolated from the skin of ‘palombeta’ (Chloroscombrus chrysurus) and ‘guaiúba’ (Ocyurus chrysurus): features and biological implications - doi: 10.4025/actascibiolsci.v34i2.6056 |
author |
Rodrigues, José Ariévilo Gurgel |
author_facet |
Rodrigues, José Ariévilo Gurgel Vanderlei, Edfranck de Sousa Oliveira Quinderé, Ana Luíza Gomes Fontes, Bruno Pedrosa Queiroz, Ismael Nilo Lino de Benevides, Norma Maria Barros |
author_role |
author |
author2 |
Vanderlei, Edfranck de Sousa Oliveira Quinderé, Ana Luíza Gomes Fontes, Bruno Pedrosa Queiroz, Ismael Nilo Lino de Benevides, Norma Maria Barros |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, José Ariévilo Gurgel Vanderlei, Edfranck de Sousa Oliveira Quinderé, Ana Luíza Gomes Fontes, Bruno Pedrosa Queiroz, Ismael Nilo Lino de Benevides, Norma Maria Barros |
dc.subject.por.fl_str_mv |
marine teleosts sulfated glycosaminoglycans blood coagulation APTT test teleósteos marinhos glicosaminoglicanos sulfatados coagulação sanguínea teste do TTPA Química de macromoléculas |
topic |
marine teleosts sulfated glycosaminoglycans blood coagulation APTT test teleósteos marinhos glicosaminoglicanos sulfatados coagulação sanguínea teste do TTPA Química de macromoléculas |
description |
A great number of pharmacological compounds is found in the Brazilian marine diversity. This study evaluated the anticoagulant potential of glycosaminoglycans (GAGs) isolated from the skin of ‘palombeta’ Chloroscombrus chrysurus and ‘guaiúba’ Ocyurus chrysurus. GAGs were extracted with crude papain in 0.1 M sodium acetate buffer (pH 5.0) containing 5 mM cysteine and 5 mM EDTA, followed by ion exchange chromatography on DEAE-cellulose column. The chemical composition (contaminant proteins and total carbohydrates) and the analysis by 0.5% agarose gel electrophoresis of fractions were also determined. Anticoagulant assays were performed by activated partial thromboplastin time (APTT) using normal human plasma and standard heparin (193.00 IU mg-1). The obtaining and fractionation procedures of GAGs were effective and similar chromatographic profiles were verified between the species. A similar mobility to dermatan sulfate was revealed for C. chrysurus. This GAG also showed a low activity of 3.30 IU mg-1. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Bioprospecção ; composição bioquímica ; relações biológicas |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6056 10.4025/actascibiolsci.v34i2.6056 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6056 |
identifier_str_mv |
10.4025/actascibiolsci.v34i2.6056 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6056/pdf |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 34 No 2 (2012); 141-148 Acta Scientiarum. Biological Sciences; v. 34 n. 2 (2012); 141-148 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317394668126208 |