In vitro enzyme activity and in vivo healing activity of the protein extract from pineapple peel

• Autor(a) principal: Rocha, Denise Alvarenga
• Data de Publicação: 2019
• Outros Autores: Queiroz, Estela de Rezende, Botelho, Lucimara Nazaré Silva, Fráguas, Rodrigo Martins, Santos, Cláudia Mendes dos, Abreu, Celeste Maria Patto, Sousa, Raimundo Vicente de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Acta Scientiarum Biological Sciences
• Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/41466
• topic: bromelain; skin lesion; enzyme activity; pineapple and healing.; Bioquímica

The pineapple is a fruit that has wide acceptance worldwide both in natural form, as industrialized. Your peel is a residue generated by food industries and from this residue can obtain a protein extract which is a good source of bromelain. This study aimed to obtain a protein extract from pineapple peel, evaluate its enzyme activity and its healing properties in skin lesions in rats. Seven animal groups were used: control, treated with 5% of protein extract, 10% of protein extract and pure protein extract; 5% of commercial bromelain, 10% of commercial bromelain and pure commercial bromelain. The animals were subjected to a tissue incision and treated for 21 days. Proteolytic and specific activities of the protein extract were 1.30 U mg-1 and 45 x 10-3 U µg-1 and, for commercial bromelain, 1.04 U mg-1 and 6 x 10-3 U µg-1, respectively. In the histology of the lesion, there was no significant difference between the control and treated groups; however, macroscopically, the prepared topical formulations assisted in the recovery of skin lesions, providing a significant reduction in their width, in the groups treated with pure protein extract, 5 and 10% commercial bromelain, and pure bromelain.