Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UERJ |
Texto Completo: | http://www.bdtd.uerj.br/handle/1/14036 |
Resumo: | The aim of this thesis was to evaluate the expression of Th1 cytokines (IL-12 and INF-γ), Th2 cytokines (IL-4, IL-6 and IL-10) and the pro-inflammatory cytokines IL-18, IL-1 and TNF-α in the gingival crevicular fluid (GCF) from Crohn s disease (CD) patients, ulcerative colitis (UC) patients and healthy individuals (control group, CG) who had chronic periodontitis. Besides, we measured elastase activity, matrix metalloproteinase -8 and -9 (MMP-8 and -9) to address the neutrophil function in the GCF. Fifteen CD patients (mean age 38.2 11.4 years), 15 UC patients (mean age 45.0 10.5 years) and 15 systemically healthy controls (mean age 42.1 7.8 years) were enrolled in this study. All the present teeth, except for the third molars were examined. Probing pocket depth (PPD), clinical attachment loss (CAL), presence of plaque and presence of bleeding on probing were assessed in six sites per tooth. In every subject, GCF from 4 gingivitis sites (PPD 3mm and CAL 1mm) and from 4 periodontitis sites (PPD 5mm and CAL 3mm) were collected with filter strips. The data were reported as total amount and concentration. IL-1, IL-4, IL-6, IL-10, IL-12p70, TNFα, INFγ, MMP-8 and MMP-9 were analyzed by the Luminex® analyzer. IL-18 was analyzed using a commercially available ELISA assay and the elastase activity by an enzymatic reaction. The serum was also analysed and the correlations between the cytokines in the GCF and in the serum were calculated by Pearson correlation analysis. In gingivitis sites, the total amount of IL-4 was significantly lower in the UC group than in the CG group (p=0.016). In periodontitis sites, the total amount of IL-4 was significantly lower in CD group than in the CG group (p=0.029). The total amount of IL-4 was lower in UC group than in CD group (p=0.077). Similarly, IL-4 concentrations in both CD (p=0.096) and UC (p=0.064) groups were lower than in CG group. IL-6 total amount (p=0.028) and IL-6 concentration (p=0.044) were significantly higher in the UC group than in the CG group. In the serum, IL-18 levels were significantly higher in CD (p=0.011) and UC (p=0.019) groups than in the CG group. In UC group, there was a positive correlation between serum IL-18 levels and IL-1 in the GCF (r=0. 667, p=0.01). In conclusion, IBD patients had lower IL-4 levels in the GCF and higher IL-18 levels in the serum than healthy controls. The neutrophil function in IBD patients is similar to controls. Except for the positive correlation between IL-18 in the serum and IL-1 in the GCF, there was no correlation between the cytokines in serum and in the GCF either in IBD patients or in controls. There was not a Th1 or Th2 polarization in the GCF from IBD patients. |
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Figueredo, Carlos Marcelo da Silvahttp://lattes.cnpq.br/0411336093247858Gustafsson, AndersDias, Katia Regina Hostilio Cervanteshttp://lattes.cnpq.br/8735933332801760Hirata Júnior, Raphaelhttp://lattes.cnpq.br/4484092525465200Fischer, Ricardo Guimarãeshttp://lattes.cnpq.br/7371151451215513Zaltman, Cyrlahttp://lattes.cnpq.br/8293200105233134Falabella, Márcio Eduardo Vieirahttp://lattes.cnpq.br/3251096622251503http://lattes.cnpq.br/1213821078892088Silva, Fernanda de Brito2021-01-07T14:57:19Z2010-04-192008-08-01SILVA, Fernanda de Brito. Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica. 2008. 76 f. Tese (Doutorado em Dentística; Endodontia; Odontopediatria; Ortodontia; Periodontia;) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2008.http://www.bdtd.uerj.br/handle/1/14036The aim of this thesis was to evaluate the expression of Th1 cytokines (IL-12 and INF-γ), Th2 cytokines (IL-4, IL-6 and IL-10) and the pro-inflammatory cytokines IL-18, IL-1 and TNF-α in the gingival crevicular fluid (GCF) from Crohn s disease (CD) patients, ulcerative colitis (UC) patients and healthy individuals (control group, CG) who had chronic periodontitis. Besides, we measured elastase activity, matrix metalloproteinase -8 and -9 (MMP-8 and -9) to address the neutrophil function in the GCF. Fifteen CD patients (mean age 38.2 11.4 years), 15 UC patients (mean age 45.0 10.5 years) and 15 systemically healthy controls (mean age 42.1 7.8 years) were enrolled in this study. All the present teeth, except for the third molars were examined. Probing pocket depth (PPD), clinical attachment loss (CAL), presence of plaque and presence of bleeding on probing were assessed in six sites per tooth. In every subject, GCF from 4 gingivitis sites (PPD 3mm and CAL 1mm) and from 4 periodontitis sites (PPD 5mm and CAL 3mm) were collected with filter strips. The data were reported as total amount and concentration. IL-1, IL-4, IL-6, IL-10, IL-12p70, TNFα, INFγ, MMP-8 and MMP-9 were analyzed by the Luminex® analyzer. IL-18 was analyzed using a commercially available ELISA assay and the elastase activity by an enzymatic reaction. The serum was also analysed and the correlations between the cytokines in the GCF and in the serum were calculated by Pearson correlation analysis. In gingivitis sites, the total amount of IL-4 was significantly lower in the UC group than in the CG group (p=0.016). In periodontitis sites, the total amount of IL-4 was significantly lower in CD group than in the CG group (p=0.029). The total amount of IL-4 was lower in UC group than in CD group (p=0.077). Similarly, IL-4 concentrations in both CD (p=0.096) and UC (p=0.064) groups were lower than in CG group. IL-6 total amount (p=0.028) and IL-6 concentration (p=0.044) were significantly higher in the UC group than in the CG group. In the serum, IL-18 levels were significantly higher in CD (p=0.011) and UC (p=0.019) groups than in the CG group. In UC group, there was a positive correlation between serum IL-18 levels and IL-1 in the GCF (r=0. 667, p=0.01). In conclusion, IBD patients had lower IL-4 levels in the GCF and higher IL-18 levels in the serum than healthy controls. The neutrophil function in IBD patients is similar to controls. Except for the positive correlation between IL-18 in the serum and IL-1 in the GCF, there was no correlation between the cytokines in serum and in the GCF either in IBD patients or in controls. There was not a Th1 or Th2 polarization in the GCF from IBD patients.O objetivo dessa tese foi avaliar a expressão de citocinas Th1 (IL-12 e INFγ), citocinas Th2 (IL-4, IL-6 e IL-10) e das citocinas pró-inflamatórias IL-18, IL-1 e TNF no fluido gengival de pacientes com periodontite crônica portadores da doença de Crohn (DC), de retocolite ulcerativa idiopática (RCUI) e em indivíduos saudáveis (o grupo controle, GC). Como objetivo secundário, avaliamos a função dos neutrófilos no fluido gengival desses pacientes através da mensuração das metaloproteinases da matriz -8, -9 (MMP-8 e MMP-9) e da atividade da elastase. Quinze pacientes com DC (idade média 38.2 ± 11.4 anos), 15 pacientes com RCUI (idade média 45.0 ± 10.5 anos) e 15 pacientes saudáveis (idade média 42.1 ± 7.8 anos) participaram desse estudo. Todos os dentes presentes, com exceção dos terceiros molares, foram examinados. Profundidade de bolsa (PB), nível de inserção clínica (NI), presença de placa e de sangramento a sondagem foram avaliados em seis sítios por dente. Em cada paciente, o fluido de 4 sítios com periodontite (PB 5 mm e NI 3mm) e de 4 sítios com gengivite (PB 3 mm e NI 1 mm) foram coletados através de pontas de papel absorvente pré-fabricadas. O sistema LUMINEX® foi utilizado na mensuração das IL-1, IL-4, IL-6, IL-10, IL-12p70, TNF, INFγ, MMP-8 e MMP-9. A IL-18 foi analisada através do ensaio ELISA e a atividade de elastase através de uma reação enzimática. O soro desses pacientes também foi analisado e o coeficiente de correlação de Pearson foi utilizado na análise da correlação entre as citocinas no soro e no fluido gengival. Nos sítios com gengivite, a quantidade total de IL-4 foi significativamente menor no grupo RCUI do que no grupo GC (p=0.016). Nos sítios com periodontite, a quantidade total de IL-4 foi significativamente menor no grupo DC do que no grupo GC (p=0.029). A quantidade total da IL-6 (p=0.028) assim como sua concentração (p=0.044) foram significativamente maiores no grupo RCUI do que no grupo GC. No soro, os níveis da IL-18 foram significativamente mais altos nos grupos DC (p=0.011) e RCUI (p=0.019) do que no grupo GC. No grupo RCUI, a IL-18 do soro se correlacionou positivamente com a IL-1 do fluido gengival (r=0. 667, p=0.01). Concluindo, nos pacientes com doença inflamatória intestinal, os níveis da IL-4 estavam mais baixos no fluido gengival e os níveis da IL-18 estavam aumentados no soro quando comparados aos controles. A função dos neutrófilos foi similar entre os pacientes com doença inflamatória intestinal e os controles. Com exceção da correlação positiva entre a IL-18 sérica e a IL-1 no fluido gengival dos pacientes com retocolite ulcerativa idiopática, não houve correlação entre as diversas citocinas mensuradas no soro e as citocinas mensuradas no fluido gengival nos controles nem nos pacientes com doença inflamatória intestinal. Não houve a caracterização de um padrão de resposta Th1 ou Th2 no fluido gengival dos pacientes com doença inflamatória intestinal.Submitted by Boris Flegr (boris@uerj.br) on 2021-01-07T14:57:19Z No. of bitstreams: 1 FINALFernanda de Brito Silva.pdf: 2876958 bytes, checksum: 71f12a0d0aa5211cd9a9c22e4286018b (MD5)Made available in DSpace on 2021-01-07T14:57:19Z (GMT). No. of bitstreams: 1 FINALFernanda de Brito Silva.pdf: 2876958 bytes, checksum: 71f12a0d0aa5211cd9a9c22e4286018b (MD5) Previous issue date: 2008-08-01application/pdfporUniversidade do Estado do Rio de JaneiroPrograma de Pós-Graduação em OdontologiaUERJBRCentro Biomédico::Faculdade de OdontologiaPeriodontitisInflammatory bowel diseaseCytokinesTh cell profileIntestinos - Doenças inflamatóriasCrohn, Doença deColite ulcerativaCitocineseCélulas TPeriodontiteDoença inflamatória intestinalCitocinasPerfil linfocitárioCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA::PERIODONTIAPerfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônicaTh cell profile in the gingival crevicular fluid from inflammatory bowel disease patients with chronic periodontitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UERJinstname:Universidade do Estado do Rio de Janeiro (UERJ)instacron:UERJORIGINALFINALFernanda de Brito Silva.pdfapplication/pdf2876958http://www.bdtd.uerj.br/bitstream/1/14036/1/FINALFernanda+de+Brito+Silva.pdf71f12a0d0aa5211cd9a9c22e4286018bMD511/140362024-02-26 20:13:12.567oai:www.bdtd.uerj.br:1/14036Biblioteca Digital de Teses e Dissertaçõeshttp://www.bdtd.uerj.br/PUBhttps://www.bdtd.uerj.br:8443/oai/requestbdtd.suporte@uerj.bropendoar:29032024-02-26T23:13:12Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ)false |
dc.title.por.fl_str_mv |
Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica |
dc.title.alternative.eng.fl_str_mv |
Th cell profile in the gingival crevicular fluid from inflammatory bowel disease patients with chronic periodontitis |
title |
Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica |
spellingShingle |
Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica Silva, Fernanda de Brito Periodontitis Inflammatory bowel disease Cytokines Th cell profile Intestinos - Doenças inflamatórias Crohn, Doença de Colite ulcerativa Citocinese Células T Periodontite Doença inflamatória intestinal Citocinas Perfil linfocitário CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA::PERIODONTIA |
title_short |
Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica |
title_full |
Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica |
title_fullStr |
Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica |
title_full_unstemmed |
Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica |
title_sort |
Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica |
author |
Silva, Fernanda de Brito |
author_facet |
Silva, Fernanda de Brito |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Figueredo, Carlos Marcelo da Silva |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0411336093247858 |
dc.contributor.advisor-co1.fl_str_mv |
Gustafsson, Anders |
dc.contributor.referee1.fl_str_mv |
Dias, Katia Regina Hostilio Cervantes |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/8735933332801760 |
dc.contributor.referee2.fl_str_mv |
Hirata Júnior, Raphael |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4484092525465200 |
dc.contributor.referee3.fl_str_mv |
Fischer, Ricardo Guimarães |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/7371151451215513 |
dc.contributor.referee4.fl_str_mv |
Zaltman, Cyrla |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/8293200105233134 |
dc.contributor.referee5.fl_str_mv |
Falabella, Márcio Eduardo Vieira |
dc.contributor.referee5Lattes.fl_str_mv |
http://lattes.cnpq.br/3251096622251503 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1213821078892088 |
dc.contributor.author.fl_str_mv |
Silva, Fernanda de Brito |
contributor_str_mv |
Figueredo, Carlos Marcelo da Silva Gustafsson, Anders Dias, Katia Regina Hostilio Cervantes Hirata Júnior, Raphael Fischer, Ricardo Guimarães Zaltman, Cyrla Falabella, Márcio Eduardo Vieira |
dc.subject.eng.fl_str_mv |
Periodontitis Inflammatory bowel disease Cytokines Th cell profile |
topic |
Periodontitis Inflammatory bowel disease Cytokines Th cell profile Intestinos - Doenças inflamatórias Crohn, Doença de Colite ulcerativa Citocinese Células T Periodontite Doença inflamatória intestinal Citocinas Perfil linfocitário CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA::PERIODONTIA |
dc.subject.por.fl_str_mv |
Intestinos - Doenças inflamatórias Crohn, Doença de Colite ulcerativa Citocinese Células T Periodontite Doença inflamatória intestinal Citocinas Perfil linfocitário |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA::PERIODONTIA |
description |
The aim of this thesis was to evaluate the expression of Th1 cytokines (IL-12 and INF-γ), Th2 cytokines (IL-4, IL-6 and IL-10) and the pro-inflammatory cytokines IL-18, IL-1 and TNF-α in the gingival crevicular fluid (GCF) from Crohn s disease (CD) patients, ulcerative colitis (UC) patients and healthy individuals (control group, CG) who had chronic periodontitis. Besides, we measured elastase activity, matrix metalloproteinase -8 and -9 (MMP-8 and -9) to address the neutrophil function in the GCF. Fifteen CD patients (mean age 38.2 11.4 years), 15 UC patients (mean age 45.0 10.5 years) and 15 systemically healthy controls (mean age 42.1 7.8 years) were enrolled in this study. All the present teeth, except for the third molars were examined. Probing pocket depth (PPD), clinical attachment loss (CAL), presence of plaque and presence of bleeding on probing were assessed in six sites per tooth. In every subject, GCF from 4 gingivitis sites (PPD 3mm and CAL 1mm) and from 4 periodontitis sites (PPD 5mm and CAL 3mm) were collected with filter strips. The data were reported as total amount and concentration. IL-1, IL-4, IL-6, IL-10, IL-12p70, TNFα, INFγ, MMP-8 and MMP-9 were analyzed by the Luminex® analyzer. IL-18 was analyzed using a commercially available ELISA assay and the elastase activity by an enzymatic reaction. The serum was also analysed and the correlations between the cytokines in the GCF and in the serum were calculated by Pearson correlation analysis. In gingivitis sites, the total amount of IL-4 was significantly lower in the UC group than in the CG group (p=0.016). In periodontitis sites, the total amount of IL-4 was significantly lower in CD group than in the CG group (p=0.029). The total amount of IL-4 was lower in UC group than in CD group (p=0.077). Similarly, IL-4 concentrations in both CD (p=0.096) and UC (p=0.064) groups were lower than in CG group. IL-6 total amount (p=0.028) and IL-6 concentration (p=0.044) were significantly higher in the UC group than in the CG group. In the serum, IL-18 levels were significantly higher in CD (p=0.011) and UC (p=0.019) groups than in the CG group. In UC group, there was a positive correlation between serum IL-18 levels and IL-1 in the GCF (r=0. 667, p=0.01). In conclusion, IBD patients had lower IL-4 levels in the GCF and higher IL-18 levels in the serum than healthy controls. The neutrophil function in IBD patients is similar to controls. Except for the positive correlation between IL-18 in the serum and IL-1 in the GCF, there was no correlation between the cytokines in serum and in the GCF either in IBD patients or in controls. There was not a Th1 or Th2 polarization in the GCF from IBD patients. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-08-01 |
dc.date.available.fl_str_mv |
2010-04-19 |
dc.date.accessioned.fl_str_mv |
2021-01-07T14:57:19Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Fernanda de Brito. Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica. 2008. 76 f. Tese (Doutorado em Dentística; Endodontia; Odontopediatria; Ortodontia; Periodontia;) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2008. |
dc.identifier.uri.fl_str_mv |
http://www.bdtd.uerj.br/handle/1/14036 |
identifier_str_mv |
SILVA, Fernanda de Brito. Perfil da resposta Th1/Th2 no fluido gengival de pacientes portadores de doença inflamatória intestinal com periodontite crônica. 2008. 76 f. Tese (Doutorado em Dentística; Endodontia; Odontopediatria; Ortodontia; Periodontia;) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2008. |
url |
http://www.bdtd.uerj.br/handle/1/14036 |
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por |
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Universidade do Estado do Rio de Janeiro |
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UERJ |
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BR |
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Centro Biomédico::Faculdade de Odontologia |
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Universidade do Estado do Rio de Janeiro |
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