A Novel Monoclonal Antibody Against Canine Monocytes/Macrophages
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2004 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/14618 |
Resumo: | Texto completo: acesso restrito. p. 250-257 |
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Santos, Roberto Robson Borges dosLarangeira, Daniela FariasAguiar, Paulo Henrique PalisSantos, Marta Almeida dosMelo, Stella Maria BarrouinSilva, Tânia Maria CorreiaMengel, José O.Santos, Washington Luis Conrado dosCarvalho, Lain Carlos Pontes deSantos, Roberto Robson Borges dosLarangeira, Daniela FariasAguiar, Paulo Henrique PalisSantos, Marta Almeida dosMelo, Stella Maria BarrouinSilva, Tânia Maria CorreiaMengel, José O.Santos, Washington Luis Conrado dosCarvalho, Lain Carlos Pontes de2014-02-13T18:35:31Z20041536-8599)http://repositorio.ufba.br/ri/handle/ri/14618v. 23, n. 4Texto completo: acesso restrito. p. 250-257The production and partial characterization of a monoclonal antibody, the IgG1 IH1, which recognizes an antigen distributed in canine monocytes/macrophages, is reported here. The distribution and apparent molecular weight of the antigen recognized by the IH1 MAb was determined in peripheral blood leukocytes, peripheral blood monocyte-derived macrophages and tissue sections of spleen, liver and skin, using Western blotting, immunocytochemistry, immunohistochemistry and flow cytometry. The IH1 MAb-recognized antigen was detected in Western blotting under non-reducing conditions spread out as a large band covering the position corresponding to the migration of molecules with molecular weights from 55 to 73 kDa. The IH1 MAb labeled blood monocytes, tissue macrophages in lymph nodes, and in the mantle zone of the spleen, and Kupffer cells in the liver. It did not react with human cells. In flow cytometric analysis, the IH1 MAb reacted with a subpopulation of monocytes. The MAb described herein may become a valuable tool for diagnosis and research on canine diseases.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-12-13T13:22:30Z No. of bitstreams: 1 Roberto Robson Borges dos Santos.pdf: 5019692 bytes, checksum: 9825d1d27bddf5c09253a29d1edb934c (MD5)Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2014-02-13T18:35:31Z (GMT) No. of bitstreams: 1 Roberto Robson Borges dos Santos.pdf: 5019692 bytes, checksum: 9825d1d27bddf5c09253a29d1edb934c (MD5)Made available in DSpace on 2014-02-13T18:35:31Z (GMT). No. of bitstreams: 1 Roberto Robson Borges dos Santos.pdf: 5019692 bytes, checksum: 9825d1d27bddf5c09253a29d1edb934c (MD5) Previous issue date: 2004http://dx.doi.org/ 10.1089/1536859041651303reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAA Novel Monoclonal Antibody Against Canine Monocytes/MacrophagesHybridoma and Hybridomicsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALRoberto Robson Borges dos Santos.pdfRoberto Robson Borges dos Santos.pdfapplication/pdf5019692https://repositorio.ufba.br/bitstream/ri/14618/1/Roberto%20Robson%20Borges%20dos%20Santos.pdf9825d1d27bddf5c09253a29d1edb934cMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14618/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTRoberto Robson Borges dos Santos.pdf.txtRoberto Robson Borges dos Santos.pdf.txtExtracted texttext/plain29821https://repositorio.ufba.br/bitstream/ri/14618/3/Roberto%20Robson%20Borges%20dos%20Santos.pdf.txt94f271d6161de2b79764f39f730bbd69MD53ri/146182022-07-05 14:02:50.39oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:02:50Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
A Novel Monoclonal Antibody Against Canine Monocytes/Macrophages |
| dc.title.alternative.pt_BR.fl_str_mv |
Hybridoma and Hybridomics |
| title |
A Novel Monoclonal Antibody Against Canine Monocytes/Macrophages |
| spellingShingle |
A Novel Monoclonal Antibody Against Canine Monocytes/Macrophages Santos, Roberto Robson Borges dos |
| title_short |
A Novel Monoclonal Antibody Against Canine Monocytes/Macrophages |
| title_full |
A Novel Monoclonal Antibody Against Canine Monocytes/Macrophages |
| title_fullStr |
A Novel Monoclonal Antibody Against Canine Monocytes/Macrophages |
| title_full_unstemmed |
A Novel Monoclonal Antibody Against Canine Monocytes/Macrophages |
| title_sort |
A Novel Monoclonal Antibody Against Canine Monocytes/Macrophages |
| author |
Santos, Roberto Robson Borges dos |
| author_facet |
Santos, Roberto Robson Borges dos Larangeira, Daniela Farias Aguiar, Paulo Henrique Palis Santos, Marta Almeida dos Melo, Stella Maria Barrouin Silva, Tânia Maria Correia Mengel, José O. Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| author_role |
author |
| author2 |
Larangeira, Daniela Farias Aguiar, Paulo Henrique Palis Santos, Marta Almeida dos Melo, Stella Maria Barrouin Silva, Tânia Maria Correia Mengel, José O. Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
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author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Roberto Robson Borges dos Larangeira, Daniela Farias Aguiar, Paulo Henrique Palis Santos, Marta Almeida dos Melo, Stella Maria Barrouin Silva, Tânia Maria Correia Mengel, José O. Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de Santos, Roberto Robson Borges dos Larangeira, Daniela Farias Aguiar, Paulo Henrique Palis Santos, Marta Almeida dos Melo, Stella Maria Barrouin Silva, Tânia Maria Correia Mengel, José O. Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| description |
Texto completo: acesso restrito. p. 250-257 |
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2004 |
| dc.date.issued.fl_str_mv |
2004 |
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2014-02-13T18:35:31Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://repositorio.ufba.br/ri/handle/ri/14618 |
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1536-8599) |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 23, n. 4 |
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1536-8599) v. 23, n. 4 |
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http://repositorio.ufba.br/ri/handle/ri/14618 |
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eng |
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openAccess |
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http://dx.doi.org/ 10.1089/1536859041651303 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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