Production of Monoclonal Antibodies Against Canine Leukocytes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2004 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/4804 |
Resumo: | p. 127-132 |
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Aguiar, Paulo Henrique PalisSantos, Roberto Robson Borges dosLima, Carla AndradeGomes, Hilton Rios de SousaLarangeira, Daniela FariasSantos, Patrícia MeiraMelo, Stella Maria BarrouinSantos, Washington Luis Conrado dosCarvalho, Lain Carlos Pontes deAguiar, Paulo Henrique PalisSantos, Roberto Robson Borges dosLima, Carla AndradeGomes, Hilton Rios de SousaLarangeira, Daniela FariasSantos, Patrícia MeiraMelo, Stella Maria BarrouinSantos, Washington Luis Conrado dosCarvalho, Lain Carlos Pontes de2011-12-12T12:39:26Z2011-12-12T12:39:26Z20041536-8599http://www.repositorio.ufba.br/ri/handle/ri/4804v. 23, n.2.p. 127-132A panel of anti-canine leukocyte monoclonal antibodies (MAbs) was produced by immunizing BALB/c mice with canine peripheral blood mononuclear cells (PBMC), either resting or stimulated with concanavalin A (ConA). Three out of 28 clones—IH1, AB6, and HG6–screened by ELISA and producing antibody with the highest specificity for canine cell immunostaining, were subjected to three subsequent subcloning steps by limiting dilution, and selected for further characterization. These MAbs belonged to IgG1 (HG6 and IH1) and IgG2a (AB6) isotypes. The distribution of cell populations expressing the antigen recognized by the antibodies was identified by indirect immunoflorescence on canine PBMC and on tissue sections of lymph node, spleen, liver and skin. The possible crossreactivity with human PBMC was also examined in immunocytochemistry. One of the antibodies specifically recognized macrophages. The MAbs presented here can be foreseen as possible valuable diagnostic and research tools to study immune functions in dogs.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2011-12-12T12:39:26Z No. of bitstreams: 1 153685904774129739.pdf: 359331 bytes, checksum: 133cf53f53b469763f1209d1c0d50ebe (MD5)Made available in DSpace on 2011-12-12T12:39:26Z (GMT). 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| dc.title.pt_BR.fl_str_mv |
Production of Monoclonal Antibodies Against Canine Leukocytes |
| dc.title.alternative.pt_BR.fl_str_mv |
Hybridoma And Hybridomics |
| title |
Production of Monoclonal Antibodies Against Canine Leukocytes |
| spellingShingle |
Production of Monoclonal Antibodies Against Canine Leukocytes Aguiar, Paulo Henrique Palis |
| title_short |
Production of Monoclonal Antibodies Against Canine Leukocytes |
| title_full |
Production of Monoclonal Antibodies Against Canine Leukocytes |
| title_fullStr |
Production of Monoclonal Antibodies Against Canine Leukocytes |
| title_full_unstemmed |
Production of Monoclonal Antibodies Against Canine Leukocytes |
| title_sort |
Production of Monoclonal Antibodies Against Canine Leukocytes |
| author |
Aguiar, Paulo Henrique Palis |
| author_facet |
Aguiar, Paulo Henrique Palis Santos, Roberto Robson Borges dos Lima, Carla Andrade Gomes, Hilton Rios de Sousa Larangeira, Daniela Farias Santos, Patrícia Meira Melo, Stella Maria Barrouin Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| author_role |
author |
| author2 |
Santos, Roberto Robson Borges dos Lima, Carla Andrade Gomes, Hilton Rios de Sousa Larangeira, Daniela Farias Santos, Patrícia Meira Melo, Stella Maria Barrouin Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
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author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Aguiar, Paulo Henrique Palis Santos, Roberto Robson Borges dos Lima, Carla Andrade Gomes, Hilton Rios de Sousa Larangeira, Daniela Farias Santos, Patrícia Meira Melo, Stella Maria Barrouin Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de Aguiar, Paulo Henrique Palis Santos, Roberto Robson Borges dos Lima, Carla Andrade Gomes, Hilton Rios de Sousa Larangeira, Daniela Farias Santos, Patrícia Meira Melo, Stella Maria Barrouin Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| description |
p. 127-132 |
| publishDate |
2004 |
| dc.date.issued.fl_str_mv |
2004 |
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2011-12-12T12:39:26Z |
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2011-12-12T12:39:26Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://www.repositorio.ufba.br/ri/handle/ri/4804 |
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1536-8599 |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 23, n.2. |
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1536-8599 v. 23, n.2. |
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http://www.repositorio.ufba.br/ri/handle/ri/4804 |
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eng |
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