Precipitation of bovine rotavirus by polyethylen glycol (PEG) and its application to produce polyclonal and monoclonal antibodies

Detalhes bibliográficos
Autor(a) principal: Fontes, Lia Vale Queiroz
Data de Publicação: 2005
Outros Autores: Campos, Gubio Soares, Beck, Patrícia Araújo, Brandão, Camila Fonseca Lopes, Sardi, Silvia Inês
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6606
Resumo: Trabalho completo: acesso restrito, p. 147–153
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spelling Fontes, Lia Vale QueirozCampos, Gubio SoaresBeck, Patrícia AraújoBrandão, Camila Fonseca LopesSardi, Silvia InêsFontes, Lia Vale QueirozCampos, Gubio SoaresBeck, Patrícia AraújoBrandão, Camila Fonseca LopesSardi, Silvia Inês2012-08-20T18:56:38Z2005-020166-0934http://www.repositorio.ufba.br/ri/handle/ri/6606v. 123, n. 2Trabalho completo: acesso restrito, p. 147–153Many protocols are available to produce monoclonal antibodies (MAbs) against bovine rotavirus (BRV) using purified virus particles but those methods are time-consuming and produce substantial loss of virus structure or infectivity. Polyethylene gycol (PEG) viral precipitation was investigated as a possible alternative method to obtain purified virus and viral antigen for monoclonal and polyclonal antibodies production. The antigenic mass inoculated into rabbits was sufficient to obtain a polyclonal hyperimmune serum with neutralizing activity and a wide range of humoral responses to different viral proteins with minimum cellular contamination. This antigenic mass inoculated into mice was capable of producing seven MAbs with different biochemical characteristics (Western-blot; indirect immunofluorescence and serum neutralization assays). When evaluated as diagnostic tools to detect BRV antigen in feces the MAbs were effective for detecting rotavirus in naturally infected calves. This work demonstrate that PEG precipitation could be an useful procedure for obtaining viral antigen to produce polyclonal and MAbs against BRV. The antigenic mass obtained, the viral infectivity, and the conserved protein pattern suggest that this methodology could be applied to this and to other viruses, reducing time or possible loss of antigens involved in viral purification.Submitted by Bruna Lessa (lessbruna@gmail.com) on 2012-08-20T18:56:38Z No. of bitstreams: 1 (171).pdf: 387024 bytes, checksum: d077ed9ed76c5913d81e557923dd32e9 (MD5)Made available in DSpace on 2012-08-20T18:56:38Z (GMT). No. of bitstreams: 1 (171).pdf: 387024 bytes, checksum: d077ed9ed76c5913d81e557923dd32e9 (MD5) Previous issue date: 2005-02Elsevierhttp://dx.doi.org/10.1016/j.jviromet.2004.09.023reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBABovine rotavirusMonoclonal antibodiesPEGPrecipitationPrecipitation of bovine rotavirus by polyethylen glycol (PEG) and its application to produce polyclonal and monoclonal antibodiesJournal of Virological Methodsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL(171).pdf(171).pdfapplication/pdf387024https://repositorio.ufba.br/bitstream/ri/6606/1/%28171%29.pdfd077ed9ed76c5913d81e557923dd32e9MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/6606/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT(171).pdf.txt(171).pdf.txtExtracted texttext/plain29873https://repositorio.ufba.br/bitstream/ri/6606/3/%28171%29.pdf.txt51e6c458c0e58ba52bb73e18f20f7b95MD53ri/66062022-07-05 14:03:32.853oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:32Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Precipitation of bovine rotavirus by polyethylen glycol (PEG) and its application to produce polyclonal and monoclonal antibodies
dc.title.alternative.pt_BR.fl_str_mv Journal of Virological Methods
title Precipitation of bovine rotavirus by polyethylen glycol (PEG) and its application to produce polyclonal and monoclonal antibodies
spellingShingle Precipitation of bovine rotavirus by polyethylen glycol (PEG) and its application to produce polyclonal and monoclonal antibodies
Fontes, Lia Vale Queiroz
Bovine rotavirus
Monoclonal antibodies
PEG
Precipitation
title_short Precipitation of bovine rotavirus by polyethylen glycol (PEG) and its application to produce polyclonal and monoclonal antibodies
title_full Precipitation of bovine rotavirus by polyethylen glycol (PEG) and its application to produce polyclonal and monoclonal antibodies
title_fullStr Precipitation of bovine rotavirus by polyethylen glycol (PEG) and its application to produce polyclonal and monoclonal antibodies
title_full_unstemmed Precipitation of bovine rotavirus by polyethylen glycol (PEG) and its application to produce polyclonal and monoclonal antibodies
title_sort Precipitation of bovine rotavirus by polyethylen glycol (PEG) and its application to produce polyclonal and monoclonal antibodies
author Fontes, Lia Vale Queiroz
author_facet Fontes, Lia Vale Queiroz
Campos, Gubio Soares
Beck, Patrícia Araújo
Brandão, Camila Fonseca Lopes
Sardi, Silvia Inês
author_role author
author2 Campos, Gubio Soares
Beck, Patrícia Araújo
Brandão, Camila Fonseca Lopes
Sardi, Silvia Inês
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Fontes, Lia Vale Queiroz
Campos, Gubio Soares
Beck, Patrícia Araújo
Brandão, Camila Fonseca Lopes
Sardi, Silvia Inês
Fontes, Lia Vale Queiroz
Campos, Gubio Soares
Beck, Patrícia Araújo
Brandão, Camila Fonseca Lopes
Sardi, Silvia Inês
dc.subject.por.fl_str_mv Bovine rotavirus
Monoclonal antibodies
PEG
Precipitation
topic Bovine rotavirus
Monoclonal antibodies
PEG
Precipitation
description Trabalho completo: acesso restrito, p. 147–153
publishDate 2005
dc.date.issued.fl_str_mv 2005-02
dc.date.accessioned.fl_str_mv 2012-08-20T18:56:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6606
dc.identifier.issn.none.fl_str_mv 0166-0934
dc.identifier.number.pt_BR.fl_str_mv v. 123, n. 2
identifier_str_mv 0166-0934
v. 123, n. 2
url http://www.repositorio.ufba.br/ri/handle/ri/6606
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.jviromet.2004.09.023
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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