Functional transcriptomics of wild-caught lutzomyia intermedia salivary glands: identification of a protective salivary protein against leishmania braziliensis infection

Detalhes bibliográficos
Autor(a) principal: Moura, Tatiana Rodrigues de
Data de Publicação: 2013
Outros Autores: Oliveira, Fabiano, Carneiro, Marcia W., Miranda, José Carlos, Clarêncio, Jorge, Barral-Netto, Manoel, Brodskyn, Claudia Ida, Barral, Aldina Maria Prado, Ribeiro, José M. C., Valenzuela, Jesus G., Oliveira, Camila Indiani de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/17657
Resumo: p. 1-16
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spelling Moura, Tatiana Rodrigues deOliveira, FabianoCarneiro, Marcia W.Miranda, José CarlosClarêncio, JorgeBarral-Netto, ManoelBrodskyn, Claudia IdaBarral, Aldina Maria PradoRibeiro, José M. C.Valenzuela, Jesus G.Oliveira, Camila Indiani deMoura, Tatiana Rodrigues deOliveira, FabianoCarneiro, Marcia W.Miranda, José CarlosClarêncio, JorgeBarral-Netto, ManoelBrodskyn, Claudia IdaBarral, Aldina Maria PradoRibeiro, José M. C.Valenzuela, Jesus G.Oliveira, Camila Indiani de2015-05-11T14:57:14Z2015-05-11T14:57:14Z20131932-6203http://repositorio.ufba.br/ri/handle/ri/17657v. 7, n. 5p. 1-16Background Leishmania parasites are transmitted in the presence of sand fly saliva. Together with the parasite, the sand fly injects salivary components that change the environment at the feeding site. Mice immunized with Phlebotomus papatasi salivary gland (SG) homogenate are protected against Leishmania major infection, while immunity to Lutzomyia intermedia SG homogenate exacerbated experimental Leishmania braziliensis infection. In humans, antibodies to Lu. intermedia saliva are associated with risk of acquiring L. braziliensis infection. Despite these important findings, there is no information regarding the repertoire of Lu. intermedia salivary proteins. Methods and Findings A cDNA library from the Salivary Glands (SGs) of wild-caught Lu. intermedia was constructed, sequenced, and complemented by a proteomic approach based on 1D SDS PAGE and mass/mass spectrometry to validate the transcripts present in this cDNA library. We identified the most abundant transcripts and proteins reported in other sand fly species as well as novel proteins such as neurotoxin-like proteins, peptides with ML domain, and three small peptides found so far only in this sand fly species. DNA plasmids coding for ten selected transcripts were constructed and used to immunize BALB/c mice to study their immunogenicity. Plasmid Linb-11—coding for a 4.5-kDa protein—induced a cellular immune response and conferred protection against L. braziliensis infection. This protection correlated with a decreased parasite load and an increased frequency of IFN-γ-producing cells. Conclusions We identified the most abundant and novel proteins present in the SGs of Lu. intermedia, a vector of cutaneous leishmaniasis in the Americas. We also show for the first time that immunity to a single salivary protein from Lu. intermedia can protect against cutaneous leishmaniasis caused by L. braziliensis.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-11-24T12:47:30Z No. of bitstreams: 1 Tatiana R. de Moura.pdf: 2866121 bytes, checksum: aef5ca382007e522140bb8e55395da18 (MD5)Approved for entry into archive by Delba Rosa (delba@ufba.br) on 2015-05-11T14:57:14Z (GMT) No. of bitstreams: 1 Tatiana R. de Moura.pdf: 2866121 bytes, checksum: aef5ca382007e522140bb8e55395da18 (MD5)Made available in DSpace on 2015-05-11T14:57:14Z (GMT). No. of bitstreams: 1 Tatiana R. de Moura.pdf: 2866121 bytes, checksum: aef5ca382007e522140bb8e55395da18 (MD5) Previous issue date: 2013http://dx.doi.org/10.1371/journal.pntd.0002242reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAFunctional transcriptomics of wild-caught lutzomyia intermedia salivary glands: identification of a protective salivary protein against leishmania braziliensis infectionPLoS ONEinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrasilinfo:eu-repo/semantics/openAccessengORIGINALTatiana R. de Moura.pdfTatiana R. de Moura.pdfapplication/pdf2866121https://repositorio.ufba.br/bitstream/ri/17657/1/Tatiana%20R.%20de%20Moura.pdfaef5ca382007e522140bb8e55395da18MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/17657/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTTatiana R. de Moura.pdf.txtTatiana R. de Moura.pdf.txtExtracted texttext/plain68803https://repositorio.ufba.br/bitstream/ri/17657/3/Tatiana%20R.%20de%20Moura.pdf.txt9a3d45170b1672fc15acc765fb8b1c76MD53ri/176572022-10-24 16:05:43.903oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-10-24T19:05:43Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Functional transcriptomics of wild-caught lutzomyia intermedia salivary glands: identification of a protective salivary protein against leishmania braziliensis infection
dc.title.alternative.pt_BR.fl_str_mv PLoS ONE
title Functional transcriptomics of wild-caught lutzomyia intermedia salivary glands: identification of a protective salivary protein against leishmania braziliensis infection
spellingShingle Functional transcriptomics of wild-caught lutzomyia intermedia salivary glands: identification of a protective salivary protein against leishmania braziliensis infection
Moura, Tatiana Rodrigues de
title_short Functional transcriptomics of wild-caught lutzomyia intermedia salivary glands: identification of a protective salivary protein against leishmania braziliensis infection
title_full Functional transcriptomics of wild-caught lutzomyia intermedia salivary glands: identification of a protective salivary protein against leishmania braziliensis infection
title_fullStr Functional transcriptomics of wild-caught lutzomyia intermedia salivary glands: identification of a protective salivary protein against leishmania braziliensis infection
title_full_unstemmed Functional transcriptomics of wild-caught lutzomyia intermedia salivary glands: identification of a protective salivary protein against leishmania braziliensis infection
title_sort Functional transcriptomics of wild-caught lutzomyia intermedia salivary glands: identification of a protective salivary protein against leishmania braziliensis infection
author Moura, Tatiana Rodrigues de
author_facet Moura, Tatiana Rodrigues de
Oliveira, Fabiano
Carneiro, Marcia W.
Miranda, José Carlos
Clarêncio, Jorge
Barral-Netto, Manoel
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Ribeiro, José M. C.
Valenzuela, Jesus G.
Oliveira, Camila Indiani de
author_role author
author2 Oliveira, Fabiano
Carneiro, Marcia W.
Miranda, José Carlos
Clarêncio, Jorge
Barral-Netto, Manoel
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Ribeiro, José M. C.
Valenzuela, Jesus G.
Oliveira, Camila Indiani de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moura, Tatiana Rodrigues de
Oliveira, Fabiano
Carneiro, Marcia W.
Miranda, José Carlos
Clarêncio, Jorge
Barral-Netto, Manoel
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Ribeiro, José M. C.
Valenzuela, Jesus G.
Oliveira, Camila Indiani de
Moura, Tatiana Rodrigues de
Oliveira, Fabiano
Carneiro, Marcia W.
Miranda, José Carlos
Clarêncio, Jorge
Barral-Netto, Manoel
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Ribeiro, José M. C.
Valenzuela, Jesus G.
Oliveira, Camila Indiani de
description p. 1-16
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-05-11T14:57:14Z
dc.date.available.fl_str_mv 2015-05-11T14:57:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/17657
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.number.pt_BR.fl_str_mv v. 7, n. 5
identifier_str_mv 1932-6203
v. 7, n. 5
url http://repositorio.ufba.br/ri/handle/ri/17657
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.country.fl_str_mv Brasil
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1371/journal.pntd.0002242
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