Lutzomyia longipalpis Saliva or Salivary Protein LJM19 Protects against Leishmania braziliensis and the Saliva of Its Vector, Lutzomyia intermedia

Detalhes bibliográficos
Autor(a) principal: Tavares, Natália Machado
Data de Publicação: 2011
Outros Autores: Silva, Robson A., Costa, Dirceu Joaquim, Pitombo, Maiana A., Fukutani, Kiyoshi Ferreira, Miranda, José C., Valenzuela, Jesus G., Barral, Aldina Maria Prado, Oliveira, Camila Indiani de, Barral-Netto, Manoel, Brodskyn, Claudia Ida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5417
Resumo: p. e1169.
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spelling Tavares, Natália MachadoSilva, Robson A.Costa, Dirceu JoaquimPitombo, Maiana A.Fukutani, Kiyoshi FerreiraMiranda, José C.Valenzuela, Jesus G.Barral, Aldina Maria PradoOliveira, Camila Indiani deBarral-Netto, ManoelBrodskyn, Claudia IdaTavares, Natália MachadoSilva, Robson A.Costa, Dirceu JoaquimPitombo, Maiana A.Fukutani, Kiyoshi FerreiraMiranda, José C.Valenzuela, Jesus G.Barral, Aldina Maria PradoOliveira, Camila Indiani deBarral-Netto, ManoelBrodskyn, Claudia Ida2012-02-14T12:03:45Z2012-02-14T12:03:45Z20111935-2727http://www.repositorio.ufba.br/ri/handle/ri/5417v. 5, n. 5.p. e1169.Background: Leishmania transmission occurs in the presence of insect saliva. Immunity to Phlebotomus papatasi or Lutzomyia longipalpis saliva or salivary components confers protection against an infection by Leishmania in the presence of the homologous saliva. However, immunization with Lutzomyia intermedia saliva did not protect mice against Leishmania braziliensis plus Lu. intermedia saliva. In the present study, we have studied whether the immunization with Lu. longipalpis saliva or a DNA plasmid coding for LJM19 salivary protein would be protective against L. braziliensis infection in the presence of Lu. intermedia saliva, the natural vector for L. braziliensis. Methodology/Principal Findings: Immunization with Lu. longipalpis saliva or with LJM19 DNA plasmid induced a Delayed-Type Hypersensitivity (DTH) response against Lu. longipalpis as well as against a Lu. intermedia saliva challenge. Immunized and unimmunized control hamsters were then intradermally infected in the ears with L. braziliensis in the presence of Lu. longipalpis or Lu. intermedia saliva. Animals immunized with Lu. longipalpis saliva exhibited smaller lesion sizes as well as reduced disease burdens both at lesion site and in the draining lymph nodes. These alterations were associated with a significant decrease in the expression levels of IL-10 and TGF-b. Animals immunized with LJM19 DNA plasmid presented similar findings in protection and immune response and additionally increased IFN-c expression.Conclusions/Significance: Immunization with Lu. longipalpis saliva or with a DNA plasmid coding LJM19 salivary protein induced protection in hamsters challenged with L. braziliensis plus Lu. intermedia saliva. These findings point out an important role of immune response against saliva components, suggesting the possibility to develop a vaccine using a single component of Lu. longipalpis saliva to generate protection against different species of Leishmania, even those transmitted by a different vector.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-02-14T12:03:45Z No. of bitstreams: 1 journal.pntd.0001169.pdf: 700787 bytes, checksum: c3ed243c40efaeea57922f38d2948f7d (MD5)Made available in DSpace on 2012-02-14T12:03:45Z (GMT). No. of bitstreams: 1 journal.pntd.0001169.pdf: 700787 bytes, checksum: c3ed243c40efaeea57922f38d2948f7d (MD5) Previous issue date: 2011http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0001169reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALutzomyia longipalpis Saliva or Salivary Protein LJM19 Protects against Leishmania braziliensis and the Saliva of Its Vector, Lutzomyia intermediaPLoS Neglected Tropical Diseasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINALjournal.pntd.0001169.pdfjournal.pntd.0001169.pdfapplication/pdf700787https://repositorio.ufba.br/bitstream/ri/5417/1/journal.pntd.0001169.pdfc3ed243c40efaeea57922f38d2948f7dMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5417/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTjournal.pntd.0001169.pdf.txtjournal.pntd.0001169.pdf.txtExtracted texttext/plain44847https://repositorio.ufba.br/bitstream/ri/5417/3/journal.pntd.0001169.pdf.txt61597a08a680484ba21c9156e0c8a36dMD53ri/54172022-07-05 14:03:44.096oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:44Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Lutzomyia longipalpis Saliva or Salivary Protein LJM19 Protects against Leishmania braziliensis and the Saliva of Its Vector, Lutzomyia intermedia
dc.title.alternative.pt_BR.fl_str_mv PLoS Neglected Tropical Diseases
title Lutzomyia longipalpis Saliva or Salivary Protein LJM19 Protects against Leishmania braziliensis and the Saliva of Its Vector, Lutzomyia intermedia
spellingShingle Lutzomyia longipalpis Saliva or Salivary Protein LJM19 Protects against Leishmania braziliensis and the Saliva of Its Vector, Lutzomyia intermedia
Tavares, Natália Machado
title_short Lutzomyia longipalpis Saliva or Salivary Protein LJM19 Protects against Leishmania braziliensis and the Saliva of Its Vector, Lutzomyia intermedia
title_full Lutzomyia longipalpis Saliva or Salivary Protein LJM19 Protects against Leishmania braziliensis and the Saliva of Its Vector, Lutzomyia intermedia
title_fullStr Lutzomyia longipalpis Saliva or Salivary Protein LJM19 Protects against Leishmania braziliensis and the Saliva of Its Vector, Lutzomyia intermedia
title_full_unstemmed Lutzomyia longipalpis Saliva or Salivary Protein LJM19 Protects against Leishmania braziliensis and the Saliva of Its Vector, Lutzomyia intermedia
title_sort Lutzomyia longipalpis Saliva or Salivary Protein LJM19 Protects against Leishmania braziliensis and the Saliva of Its Vector, Lutzomyia intermedia
author Tavares, Natália Machado
author_facet Tavares, Natália Machado
Silva, Robson A.
Costa, Dirceu Joaquim
Pitombo, Maiana A.
Fukutani, Kiyoshi Ferreira
Miranda, José C.
Valenzuela, Jesus G.
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
Barral-Netto, Manoel
Brodskyn, Claudia Ida
author_role author
author2 Silva, Robson A.
Costa, Dirceu Joaquim
Pitombo, Maiana A.
Fukutani, Kiyoshi Ferreira
Miranda, José C.
Valenzuela, Jesus G.
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
Barral-Netto, Manoel
Brodskyn, Claudia Ida
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tavares, Natália Machado
Silva, Robson A.
Costa, Dirceu Joaquim
Pitombo, Maiana A.
Fukutani, Kiyoshi Ferreira
Miranda, José C.
Valenzuela, Jesus G.
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
Barral-Netto, Manoel
Brodskyn, Claudia Ida
Tavares, Natália Machado
Silva, Robson A.
Costa, Dirceu Joaquim
Pitombo, Maiana A.
Fukutani, Kiyoshi Ferreira
Miranda, José C.
Valenzuela, Jesus G.
Barral, Aldina Maria Prado
Oliveira, Camila Indiani de
Barral-Netto, Manoel
Brodskyn, Claudia Ida
description p. e1169.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2012-02-14T12:03:45Z
dc.date.available.fl_str_mv 2012-02-14T12:03:45Z
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dc.identifier.issn.none.fl_str_mv 1935-2727
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identifier_str_mv 1935-2727
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