Towards a more precise serological diagnosis of human Tegumentary Leishmaniasis using Leishmania Recombinant Proteins

Detalhes bibliográficos
Autor(a) principal: Souza, Ana Paula Almeida de
Data de Publicação: 2013
Outros Autores: Soto, Manuel, Costa, Jackson M. L., Boaventura, Viviane Sampaio, Oliveira, Camila Indiani de, Cristal, Juqueline Rocha, Barral-Netto, Manoel, Barral, Aldina Maria Prado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/16397
Resumo: p. 1-6
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spelling Souza, Ana Paula Almeida deSoto, ManuelCosta, Jackson M. L.Boaventura, Viviane SampaioOliveira, Camila Indiani deCristal, Juqueline RochaBarral-Netto, ManoelBarral, Aldina Maria PradoSouza, Ana Paula Almeida deSoto, ManuelCosta, Jackson M. L.Boaventura, Viviane SampaioOliveira, Camila Indiani deCristal, Juqueline RochaBarral-Netto, ManoelBarral, Aldina Maria Prado2014-10-10T15:15:48Z2014-10-10T15:15:48Z20131932-6203http://repositorio.ufba.br/ri/handle/ri/16397v. 8, n. 6p. 1-6Exposure to Leishmania induces a humoral immune response that can be used as a marker of parasite exposure. Methodology/Principal Findings: Herein, ELISA was used to screen sera from patients with Tegumentary Leishmaniasis (TL) against different L. infantum-chagasi-derived recombinant proteins (rHSP70, rH2A, rH2B, rH3, rH4 and rKMP11). Among the recombinant proteins, rHSP70 and rH2A showed the best reactivity against human sera obtained from endemic areas of TL. Receiver-Operator Characteristics (ROC) curve analysis was used to identify the effectiveness of these proteins for serodiagnosis of TL. ROC curves confirmed the superior performance of rHSP70 and rH2A, in comparison to the other tested recombinant proteins. Additionally, we evaluated the specificity of the response to rHSP70 and rH2A by testing sera obtained from patients with Chagas' disease, Tuberculosis, Leprosy or Systemic Lupus Erythematosus. In this case, rHSP70 displayed an increased ability to discriminate diseases, in comparison to SLA. Conclusion: Our results raise possibility of using rHSP70 for the serodiagnosis of TLSubmitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-06-05T14:23:47Z No. of bitstreams: 1 journal.pone.0066110.pdf: 2195240 bytes, checksum: 984373ca555cc12b6642a1a82c251d56 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2014-10-10T15:15:48Z (GMT) No. of bitstreams: 1 journal.pone.0066110.pdf: 2195240 bytes, checksum: 984373ca555cc12b6642a1a82c251d56 (MD5)Made available in DSpace on 2014-10-10T15:15:48Z (GMT). No. of bitstreams: 1 journal.pone.0066110.pdf: 2195240 bytes, checksum: 984373ca555cc12b6642a1a82c251d56 (MD5) Previous issue date: 2013http://dx.doi.org/10.1371/journal.pone.0066110reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBATowards a more precise serological diagnosis of human Tegumentary Leishmaniasis using Leishmania Recombinant ProteinsPLoS ONEinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrasilinfo:eu-repo/semantics/openAccessengORIGINALjournal.pone.0066110.pdfjournal.pone.0066110.pdfapplication/pdf2195240https://repositorio.ufba.br/bitstream/ri/16397/1/journal.pone.0066110.pdf984373ca555cc12b6642a1a82c251d56MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/16397/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXTjournal.pone.0066110.pdf.txtjournal.pone.0066110.pdf.txtExtracted texttext/plain40568https://repositorio.ufba.br/bitstream/ri/16397/3/journal.pone.0066110.pdf.txt54808f9e12e91869371757bb874c4215MD53ri/163972022-07-05 14:05:18.435oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:05:18Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Towards a more precise serological diagnosis of human Tegumentary Leishmaniasis using Leishmania Recombinant Proteins
dc.title.alternative.pt_BR.fl_str_mv PLoS ONE
title Towards a more precise serological diagnosis of human Tegumentary Leishmaniasis using Leishmania Recombinant Proteins
spellingShingle Towards a more precise serological diagnosis of human Tegumentary Leishmaniasis using Leishmania Recombinant Proteins
Souza, Ana Paula Almeida de
title_short Towards a more precise serological diagnosis of human Tegumentary Leishmaniasis using Leishmania Recombinant Proteins
title_full Towards a more precise serological diagnosis of human Tegumentary Leishmaniasis using Leishmania Recombinant Proteins
title_fullStr Towards a more precise serological diagnosis of human Tegumentary Leishmaniasis using Leishmania Recombinant Proteins
title_full_unstemmed Towards a more precise serological diagnosis of human Tegumentary Leishmaniasis using Leishmania Recombinant Proteins
title_sort Towards a more precise serological diagnosis of human Tegumentary Leishmaniasis using Leishmania Recombinant Proteins
author Souza, Ana Paula Almeida de
author_facet Souza, Ana Paula Almeida de
Soto, Manuel
Costa, Jackson M. L.
Boaventura, Viviane Sampaio
Oliveira, Camila Indiani de
Cristal, Juqueline Rocha
Barral-Netto, Manoel
Barral, Aldina Maria Prado
author_role author
author2 Soto, Manuel
Costa, Jackson M. L.
Boaventura, Viviane Sampaio
Oliveira, Camila Indiani de
Cristal, Juqueline Rocha
Barral-Netto, Manoel
Barral, Aldina Maria Prado
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Ana Paula Almeida de
Soto, Manuel
Costa, Jackson M. L.
Boaventura, Viviane Sampaio
Oliveira, Camila Indiani de
Cristal, Juqueline Rocha
Barral-Netto, Manoel
Barral, Aldina Maria Prado
Souza, Ana Paula Almeida de
Soto, Manuel
Costa, Jackson M. L.
Boaventura, Viviane Sampaio
Oliveira, Camila Indiani de
Cristal, Juqueline Rocha
Barral-Netto, Manoel
Barral, Aldina Maria Prado
description p. 1-6
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-10-10T15:15:48Z
dc.date.available.fl_str_mv 2014-10-10T15:15:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/16397
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.number.pt_BR.fl_str_mv v. 8, n. 6
identifier_str_mv 1932-6203
v. 8, n. 6
url http://repositorio.ufba.br/ri/handle/ri/16397
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.country.fl_str_mv Brasil
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0066110
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