Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study

Detalhes bibliográficos
Autor(a) principal: Moura, Kátia Vale Monteiro de
Data de Publicação: 2009
Outros Autores: Lopes, Cibelle Barbosa, Soares, Cristina Pacheco, Pinheiro, Antonio Luiz Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13440
Resumo: Texto completo: acesso restrito. p. 441-446
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spelling Moura, Kátia Vale Monteiro deLopes, Cibelle BarbosaSoares, Cristina PachecoPinheiro, Antonio Luiz BarbosaMoura, Kátia Vale Monteiro deLopes, Cibelle BarbosaSoares, Cristina PachecoPinheiro, Antonio Luiz Barbosa2013-10-31T18:17:46Z20091549-5418http://repositorio.ufba.br/ri/handle/ri/13440v. 27, n. 3Texto completo: acesso restrito. p. 441-446Objective: The aim of this study was to evaluate the effects on Hep.2 cells originating from laryngeal carcinomas, and L929 cells originating from a fibroblast line, subjected to polarized light at a wavelength of 400–2000 nm. Background Data: Recently there has been increased interest in the propagation of polarized light in randomly scattering media such as biological tissues, because of its potential applications in medicine. Materials and Methods: Irradiation was performed at two time points: T0 (24 h after cell culture) and T48 (48 h after the first irradiation). Cellular viability was assessed using an MTT assay at the following times: T0 (first irradiation), T6 (6 h after the first irradiation), T12 (12 h after the first irradiation), T24 (24 h after the first irradiation), T48 (48 h after the first irradiation), and T72 (72 h after the first irradiation). The results were analyzed using Graphpad Prism software. Results: The results showed that time influenced the cellular viability of L929 cells of both control (p = 0.0014) and illuminated cultures (p = 0.0035). Significant differences between control cells (p = 0.0001) and illuminated Hep.2 cells (p = 0.0001) were observed. There was a significant difference between the proliferation of the two types of cells illuminated compared to their controls: Hep.2 (p = 0.0001) and L929 (p = 0.0002). Conclusion: The use of polarized light on Hep.2 and L929 cells resulted in photobiological effects that need further investigation, as this is the first study using this methodology.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-10-08T15:35:53Z No. of bitstreams: 1 pho%2E2008%2E2285.pdf: 5870904 bytes, checksum: 025c482fd68228ddefdb31da312ac9e5 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-10-31T18:17:46Z (GMT) No. of bitstreams: 1 pho%2E2008%2E2285.pdf: 5870904 bytes, checksum: 025c482fd68228ddefdb31da312ac9e5 (MD5)Made available in DSpace on 2013-10-31T18:17:46Z (GMT). No. of bitstreams: 1 pho%2E2008%2E2285.pdf: 5870904 bytes, checksum: 025c482fd68228ddefdb31da312ac9e5 (MD5) Previous issue date: 2009http://dx.doi.org/10.1089/pho.2008.2285reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAEffects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro studyPhotomedicine and Laser Surgeryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALpho%2E2008%2E2285.pdfpho%2E2008%2E2285.pdfapplication/pdf5870904https://repositorio.ufba.br/bitstream/ri/13440/1/pho%252E2008%252E2285.pdf025c482fd68228ddefdb31da312ac9e5MD51LICENSElicense.txtlicense.txttext/plain1365https://repositorio.ufba.br/bitstream/ri/13440/2/license.txt5371a150bdc863f78dcf39281543bd86MD52TEXTpho%2E2008%2E2285.pdf.txtpho%2E2008%2E2285.pdf.txtExtracted texttext/plain27079https://repositorio.ufba.br/bitstream/ri/13440/3/pho%252E2008%252E2285.pdf.txt111fabdec56ffb04ff653e32c0c48da5MD53ri/134402022-07-05 14:02:48.809oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:02:48Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study
dc.title.alternative.pt_BR.fl_str_mv Photomedicine and Laser Surgery
title Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study
spellingShingle Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study
Moura, Kátia Vale Monteiro de
title_short Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study
title_full Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study
title_fullStr Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study
title_full_unstemmed Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study
title_sort Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study
author Moura, Kátia Vale Monteiro de
author_facet Moura, Kátia Vale Monteiro de
Lopes, Cibelle Barbosa
Soares, Cristina Pacheco
Pinheiro, Antonio Luiz Barbosa
author_role author
author2 Lopes, Cibelle Barbosa
Soares, Cristina Pacheco
Pinheiro, Antonio Luiz Barbosa
author2_role author
author
author
dc.contributor.author.fl_str_mv Moura, Kátia Vale Monteiro de
Lopes, Cibelle Barbosa
Soares, Cristina Pacheco
Pinheiro, Antonio Luiz Barbosa
Moura, Kátia Vale Monteiro de
Lopes, Cibelle Barbosa
Soares, Cristina Pacheco
Pinheiro, Antonio Luiz Barbosa
description Texto completo: acesso restrito. p. 441-446
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2013-10-31T18:17:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13440
dc.identifier.issn.none.fl_str_mv 1549-5418
dc.identifier.number.pt_BR.fl_str_mv v. 27, n. 3
identifier_str_mv 1549-5418
v. 27, n. 3
url http://repositorio.ufba.br/ri/handle/ri/13440
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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