Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/13440 |
Resumo: | Texto completo: acesso restrito. p. 441-446 |
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Moura, Kátia Vale Monteiro deLopes, Cibelle BarbosaSoares, Cristina PachecoPinheiro, Antonio Luiz BarbosaMoura, Kátia Vale Monteiro deLopes, Cibelle BarbosaSoares, Cristina PachecoPinheiro, Antonio Luiz Barbosa2013-10-31T18:17:46Z20091549-5418http://repositorio.ufba.br/ri/handle/ri/13440v. 27, n. 3Texto completo: acesso restrito. p. 441-446Objective: The aim of this study was to evaluate the effects on Hep.2 cells originating from laryngeal carcinomas, and L929 cells originating from a fibroblast line, subjected to polarized light at a wavelength of 400–2000 nm. Background Data: Recently there has been increased interest in the propagation of polarized light in randomly scattering media such as biological tissues, because of its potential applications in medicine. Materials and Methods: Irradiation was performed at two time points: T0 (24 h after cell culture) and T48 (48 h after the first irradiation). Cellular viability was assessed using an MTT assay at the following times: T0 (first irradiation), T6 (6 h after the first irradiation), T12 (12 h after the first irradiation), T24 (24 h after the first irradiation), T48 (48 h after the first irradiation), and T72 (72 h after the first irradiation). The results were analyzed using Graphpad Prism software. Results: The results showed that time influenced the cellular viability of L929 cells of both control (p = 0.0014) and illuminated cultures (p = 0.0035). Significant differences between control cells (p = 0.0001) and illuminated Hep.2 cells (p = 0.0001) were observed. There was a significant difference between the proliferation of the two types of cells illuminated compared to their controls: Hep.2 (p = 0.0001) and L929 (p = 0.0002). Conclusion: The use of polarized light on Hep.2 and L929 cells resulted in photobiological effects that need further investigation, as this is the first study using this methodology.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-10-08T15:35:53Z No. of bitstreams: 1 pho%2E2008%2E2285.pdf: 5870904 bytes, checksum: 025c482fd68228ddefdb31da312ac9e5 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-10-31T18:17:46Z (GMT) No. of bitstreams: 1 pho%2E2008%2E2285.pdf: 5870904 bytes, checksum: 025c482fd68228ddefdb31da312ac9e5 (MD5)Made available in DSpace on 2013-10-31T18:17:46Z (GMT). No. of bitstreams: 1 pho%2E2008%2E2285.pdf: 5870904 bytes, checksum: 025c482fd68228ddefdb31da312ac9e5 (MD5) Previous issue date: 2009http://dx.doi.org/10.1089/pho.2008.2285reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAEffects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro studyPhotomedicine and Laser Surgeryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALpho%2E2008%2E2285.pdfpho%2E2008%2E2285.pdfapplication/pdf5870904https://repositorio.ufba.br/bitstream/ri/13440/1/pho%252E2008%252E2285.pdf025c482fd68228ddefdb31da312ac9e5MD51LICENSElicense.txtlicense.txttext/plain1365https://repositorio.ufba.br/bitstream/ri/13440/2/license.txt5371a150bdc863f78dcf39281543bd86MD52TEXTpho%2E2008%2E2285.pdf.txtpho%2E2008%2E2285.pdf.txtExtracted texttext/plain27079https://repositorio.ufba.br/bitstream/ri/13440/3/pho%252E2008%252E2285.pdf.txt111fabdec56ffb04ff653e32c0c48da5MD53ri/134402022-07-05 14:02:48.809oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:02:48Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
dc.title.pt_BR.fl_str_mv |
Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study |
dc.title.alternative.pt_BR.fl_str_mv |
Photomedicine and Laser Surgery |
title |
Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study |
spellingShingle |
Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study Moura, Kátia Vale Monteiro de |
title_short |
Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study |
title_full |
Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study |
title_fullStr |
Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study |
title_full_unstemmed |
Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study |
title_sort |
Effects of a polarized light source (400-2000nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study |
author |
Moura, Kátia Vale Monteiro de |
author_facet |
Moura, Kátia Vale Monteiro de Lopes, Cibelle Barbosa Soares, Cristina Pacheco Pinheiro, Antonio Luiz Barbosa |
author_role |
author |
author2 |
Lopes, Cibelle Barbosa Soares, Cristina Pacheco Pinheiro, Antonio Luiz Barbosa |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Moura, Kátia Vale Monteiro de Lopes, Cibelle Barbosa Soares, Cristina Pacheco Pinheiro, Antonio Luiz Barbosa Moura, Kátia Vale Monteiro de Lopes, Cibelle Barbosa Soares, Cristina Pacheco Pinheiro, Antonio Luiz Barbosa |
description |
Texto completo: acesso restrito. p. 441-446 |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2013-10-31T18:17:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufba.br/ri/handle/ri/13440 |
dc.identifier.issn.none.fl_str_mv |
1549-5418 |
dc.identifier.number.pt_BR.fl_str_mv |
v. 27, n. 3 |
identifier_str_mv |
1549-5418 v. 27, n. 3 |
url |
http://repositorio.ufba.br/ri/handle/ri/13440 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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http://dx.doi.org/10.1089/pho.2008.2285 |
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