Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosy

Detalhes bibliográficos
Autor(a) principal: Martins, Marcia V. S. B.
Data de Publicação: 2012
Outros Autores: Guimarães, Marjorie M. da S., Spencer, John S., Hacker, Mariana A. V. B., Costa, Luciana S., Carvalho, Fernanda M., Geluk, Annemieke, Schip, Jolien J. van der Ploeg-van, Pontes, Maria A. A., Gonçalves, Heitor S., Morais, Janvier P. de, Bandeira, Tereza de Jesus Pinheiro Gomes, Pessolani, Maria C. V., Brennan, Patrick J., Pereira, Geraldo M. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da Universidade Federal do Ceará (UFC)
Texto Completo: http://www.repositorio.ufc.br/handle/riufc/5315
Resumo: During recent years, comparative genomic analysis has allowed the identification of Mycobacterium leprae -specific genes with potential application for the diagnosis of leprosy. In a previous study, 58 synthetic peptides derived from these sequences were tested for their ability to induce production of IFN- c in PBMC from endemic controls (EC) with unknown exposure to M. leprae , household contacts of leprosy patients and patients, indicating the potential of these synthetic peptides for the diagnosis of sub- or preclinical forms of leprosy. In the present study, the patterns of IFN- c release of the individuals exposed or non-exposed to M. leprae were compared using an Artificial Neural Network algorithm, and the most promising M. leprae peptides for the identification of exposed people were selected. This subset of M. leprae -specific peptides allowed the differentiation of groups of individuals from sites hyperendemic for leprosy versus those from areas with lower level detection rates. A progressive reduction in the IFN- c levels in response to the peptides was seen when contacts of multibacillary (MB) patients were compared to other less exposed groups, suggesting a down modulation of IFN- c production with an increase in bacillary load or exposure to M. leprae . The data generated indicate that an IFN- c assay based on these peptides applied individually or as a pool can be used as a new tool for predicting the magnitude of M. leprae transmission in a given population.
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spelling Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosyHanseníaseMycobacterium lepraeDuring recent years, comparative genomic analysis has allowed the identification of Mycobacterium leprae -specific genes with potential application for the diagnosis of leprosy. In a previous study, 58 synthetic peptides derived from these sequences were tested for their ability to induce production of IFN- c in PBMC from endemic controls (EC) with unknown exposure to M. leprae , household contacts of leprosy patients and patients, indicating the potential of these synthetic peptides for the diagnosis of sub- or preclinical forms of leprosy. In the present study, the patterns of IFN- c release of the individuals exposed or non-exposed to M. leprae were compared using an Artificial Neural Network algorithm, and the most promising M. leprae peptides for the identification of exposed people were selected. This subset of M. leprae -specific peptides allowed the differentiation of groups of individuals from sites hyperendemic for leprosy versus those from areas with lower level detection rates. A progressive reduction in the IFN- c levels in response to the peptides was seen when contacts of multibacillary (MB) patients were compared to other less exposed groups, suggesting a down modulation of IFN- c production with an increase in bacillary load or exposure to M. leprae . The data generated indicate that an IFN- c assay based on these peptides applied individually or as a pool can be used as a new tool for predicting the magnitude of M. leprae transmission in a given population.PLoS Neglected Tropical Diseases2013-07-10T10:59:42Z2013-07-10T10:59:42Z2012-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMARTINS, M. V. S. B. et al. Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosy. PLoS Neglected Tropical Diseases, San Francisco, v. 6, n. 4, p. e1616, abr. 2012.1935-2727http://www.repositorio.ufc.br/handle/riufc/5315Martins, Marcia V. S. B.Guimarães, Marjorie M. da S.Spencer, John S.Hacker, Mariana A. V. B.Costa, Luciana S.Carvalho, Fernanda M.Geluk, AnnemiekeSchip, Jolien J. van der Ploeg-vanPontes, Maria A. A.Gonçalves, Heitor S.Morais, Janvier P. deBandeira, Tereza de Jesus Pinheiro GomesPessolani, Maria C. V.Brennan, Patrick J.Pereira, Geraldo M. B.engreponame:Repositório Institucional da Universidade Federal do Ceará (UFC)instname:Universidade Federal do Ceará (UFC)instacron:UFCinfo:eu-repo/semantics/openAccess2022-12-09T12:12:27Zoai:repositorio.ufc.br:riufc/5315Repositório InstitucionalPUBhttp://www.repositorio.ufc.br/ri-oai/requestbu@ufc.br || repositorio@ufc.bropendoar:2024-09-11T18:40:38.871557Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)false
dc.title.none.fl_str_mv Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosy
title Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosy
spellingShingle Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosy
Martins, Marcia V. S. B.
Hanseníase
Mycobacterium leprae
title_short Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosy
title_full Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosy
title_fullStr Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosy
title_full_unstemmed Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosy
title_sort Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosy
author Martins, Marcia V. S. B.
author_facet Martins, Marcia V. S. B.
Guimarães, Marjorie M. da S.
Spencer, John S.
Hacker, Mariana A. V. B.
Costa, Luciana S.
Carvalho, Fernanda M.
Geluk, Annemieke
Schip, Jolien J. van der Ploeg-van
Pontes, Maria A. A.
Gonçalves, Heitor S.
Morais, Janvier P. de
Bandeira, Tereza de Jesus Pinheiro Gomes
Pessolani, Maria C. V.
Brennan, Patrick J.
Pereira, Geraldo M. B.
author_role author
author2 Guimarães, Marjorie M. da S.
Spencer, John S.
Hacker, Mariana A. V. B.
Costa, Luciana S.
Carvalho, Fernanda M.
Geluk, Annemieke
Schip, Jolien J. van der Ploeg-van
Pontes, Maria A. A.
Gonçalves, Heitor S.
Morais, Janvier P. de
Bandeira, Tereza de Jesus Pinheiro Gomes
Pessolani, Maria C. V.
Brennan, Patrick J.
Pereira, Geraldo M. B.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Martins, Marcia V. S. B.
Guimarães, Marjorie M. da S.
Spencer, John S.
Hacker, Mariana A. V. B.
Costa, Luciana S.
Carvalho, Fernanda M.
Geluk, Annemieke
Schip, Jolien J. van der Ploeg-van
Pontes, Maria A. A.
Gonçalves, Heitor S.
Morais, Janvier P. de
Bandeira, Tereza de Jesus Pinheiro Gomes
Pessolani, Maria C. V.
Brennan, Patrick J.
Pereira, Geraldo M. B.
dc.subject.por.fl_str_mv Hanseníase
Mycobacterium leprae
topic Hanseníase
Mycobacterium leprae
description During recent years, comparative genomic analysis has allowed the identification of Mycobacterium leprae -specific genes with potential application for the diagnosis of leprosy. In a previous study, 58 synthetic peptides derived from these sequences were tested for their ability to induce production of IFN- c in PBMC from endemic controls (EC) with unknown exposure to M. leprae , household contacts of leprosy patients and patients, indicating the potential of these synthetic peptides for the diagnosis of sub- or preclinical forms of leprosy. In the present study, the patterns of IFN- c release of the individuals exposed or non-exposed to M. leprae were compared using an Artificial Neural Network algorithm, and the most promising M. leprae peptides for the identification of exposed people were selected. This subset of M. leprae -specific peptides allowed the differentiation of groups of individuals from sites hyperendemic for leprosy versus those from areas with lower level detection rates. A progressive reduction in the IFN- c levels in response to the peptides was seen when contacts of multibacillary (MB) patients were compared to other less exposed groups, suggesting a down modulation of IFN- c production with an increase in bacillary load or exposure to M. leprae . The data generated indicate that an IFN- c assay based on these peptides applied individually or as a pool can be used as a new tool for predicting the magnitude of M. leprae transmission in a given population.
publishDate 2012
dc.date.none.fl_str_mv 2012-04
2013-07-10T10:59:42Z
2013-07-10T10:59:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv MARTINS, M. V. S. B. et al. Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosy. PLoS Neglected Tropical Diseases, San Francisco, v. 6, n. 4, p. e1616, abr. 2012.
1935-2727
http://www.repositorio.ufc.br/handle/riufc/5315
identifier_str_mv MARTINS, M. V. S. B. et al. Pathogen-specific epitopes as epidemiological tools for defining the magnitude of mycobacterium leprae transmission in areas endemic for leprosy. PLoS Neglected Tropical Diseases, San Francisco, v. 6, n. 4, p. e1616, abr. 2012.
1935-2727
url http://www.repositorio.ufc.br/handle/riufc/5315
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLoS Neglected Tropical Diseases
publisher.none.fl_str_mv PLoS Neglected Tropical Diseases
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal do Ceará (UFC)
instname:Universidade Federal do Ceará (UFC)
instacron:UFC
instname_str Universidade Federal do Ceará (UFC)
instacron_str UFC
institution UFC
reponame_str Repositório Institucional da Universidade Federal do Ceará (UFC)
collection Repositório Institucional da Universidade Federal do Ceará (UFC)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal do Ceará (UFC) - Universidade Federal do Ceará (UFC)
repository.mail.fl_str_mv bu@ufc.br || repositorio@ufc.br
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