Growth regulators alter the development and metabolism of lemon balm seedlings cultured in vitro

Detalhes bibliográficos
Autor(a) principal: Silva, Leila Isabel da
Data de Publicação: 2023
Outros Autores: Magalhães, Hélida Mara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Caatinga
Texto Completo: https://periodicos.ufersa.edu.br/caatinga/article/view/11072
Resumo: Melissa officinalis L., popularly known as lemon balm, is an aromatic plant widely used in medicine, cosmetics, and pharmaceutical industries for its essential oil rich in phenylpropanoids, terpenes, and phenolics. This study aimed to assess the effect of growth regulators on the development and physiological and biochemical metabolism of M. officinalis cultured in vitro. Seeds were inoculated in Murashige and Skoog medium and added with the regulators 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) according to six different treatments. After 90 days of culture, plants were evaluated for growth and biochemical and physiological parameters (flavonoids, anthocyanins, and chlorophyll). The balance between regulators interfered with plant growth, which increased in the presence of 0.2 mg L−1 BAP. In this treatment, the plants had greater growth with more leaves, and the biomass production of shoots and roots was higher than the control. Growth regulators did not influence nitrogen assimilation or flavonoid production; however, total chlorophyll and anthocyanin indexes were enhanced by treatment with BAP at concentrations ranging from 0.2 to 0.5 mg L−1. Auxin treatment did not improve root production or growth but favored callus formation when combined with 0.5–3.0 mg L−1 BAP. The results indicate that high BAP concentrations (above 1.0 mg L−1) should not be used in in vitro production of lemon balm.
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spelling Growth regulators alter the development and metabolism of lemon balm seedlings cultured in vitroReguladores de crescimento promovem alterações no desenvolvimento e no metabolismo de plântulas de melissa cultivadas in vitroAntocianinas. Clorofilas. Lamiaceae. Micropropagação.Anthocyanins. Chlorophyll. Lamiaceae. Micropropagation.Melissa officinalis L., popularly known as lemon balm, is an aromatic plant widely used in medicine, cosmetics, and pharmaceutical industries for its essential oil rich in phenylpropanoids, terpenes, and phenolics. This study aimed to assess the effect of growth regulators on the development and physiological and biochemical metabolism of M. officinalis cultured in vitro. Seeds were inoculated in Murashige and Skoog medium and added with the regulators 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) according to six different treatments. After 90 days of culture, plants were evaluated for growth and biochemical and physiological parameters (flavonoids, anthocyanins, and chlorophyll). The balance between regulators interfered with plant growth, which increased in the presence of 0.2 mg L−1 BAP. In this treatment, the plants had greater growth with more leaves, and the biomass production of shoots and roots was higher than the control. Growth regulators did not influence nitrogen assimilation or flavonoid production; however, total chlorophyll and anthocyanin indexes were enhanced by treatment with BAP at concentrations ranging from 0.2 to 0.5 mg L−1. Auxin treatment did not improve root production or growth but favored callus formation when combined with 0.5–3.0 mg L−1 BAP. The results indicate that high BAP concentrations (above 1.0 mg L−1) should not be used in in vitro production of lemon balm.A espécie Melissa officinalis L. conhecida como erva cidreira é uma planta aromática muito utilizada na medicina, indústria de cosméticos e farmacêutica, em função do seu óleo essencial rico em fenilpropanóides, terpenos e fenólicos. Objetivou-se com esta pesquisa avaliar a ação dos reguladores de crescimento sobre o crescimento, processos fisiológicos e bioquímicos de plantas de M. officinalis cultivadas in vitro. Sementes foram inoculadas em meio de cultura Murashige e Skoog com os reguladores benzilaminopurina - BAP e ácido 1-naftalenoacético - ANA em seis diferentes tratamentos. Ao final de 90 dias foram avaliados: crescimento da planta, índices bioquímicos e fisiológicos sendo flavonoides, antocianinas e clorofila. O balanço entre os reguladores interferiu no crescimento da melissa que foi incrementado em            0,2 mg L−1 de BAP. Neste tratamento, as plantas tiveram maior crescimento com mais folhas como também a produção de biomassa das brotações e das raízes foi maior comparada ao controle. Os reguladores de crescimento não influenciaram na assimilação de nitrogênio e na produção de flavonoides, no entanto as clorofilas totais e as antocianinas foram incrementadas com a adição de BAP na faixa de 0,2 a 0,5 mg L−1. A adição de auxinas não melhorou aspectos de produção e crescimento da raiz, mas favoreceu a formação de calos quando foi combinada com o BAP a 0,5 e 3,0 mg L−1. Portanto, concentrações acima 1,0 mg L−1 não devem ser utilizadas no cultivo in vitro da melissa.Universidade Federal Rural do Semi-Árido2023-02-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufersa.edu.br/caatinga/article/view/1107210.1590/1983-21252023v36n214rcREVISTA CAATINGA; Vol. 36 No. 2 (2023); 372-380Revista Caatinga; v. 36 n. 2 (2023); 372-3801983-21250100-316Xreponame:Revista Caatingainstname:Universidade Federal Rural do Semi-Árido (UFERSA)instacron:UFERSAenghttps://periodicos.ufersa.edu.br/caatinga/article/view/11072/11146Copyright (c) 2023 Revista Caatingainfo:eu-repo/semantics/openAccessSilva, Leila Isabel daMagalhães, Hélida Mara2023-07-27T11:58:29Zoai:ojs.periodicos.ufersa.edu.br:article/11072Revistahttps://periodicos.ufersa.edu.br/index.php/caatinga/indexPUBhttps://periodicos.ufersa.edu.br/index.php/caatinga/oaipatricio@ufersa.edu.br|| caatinga@ufersa.edu.br1983-21250100-316Xopendoar:2024-04-29T09:47:01.452620Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)true
dc.title.none.fl_str_mv Growth regulators alter the development and metabolism of lemon balm seedlings cultured in vitro
Reguladores de crescimento promovem alterações no desenvolvimento e no metabolismo de plântulas de melissa cultivadas in vitro
title Growth regulators alter the development and metabolism of lemon balm seedlings cultured in vitro
spellingShingle Growth regulators alter the development and metabolism of lemon balm seedlings cultured in vitro
Silva, Leila Isabel da
Antocianinas. Clorofilas. Lamiaceae. Micropropagação.
Anthocyanins. Chlorophyll. Lamiaceae. Micropropagation.
title_short Growth regulators alter the development and metabolism of lemon balm seedlings cultured in vitro
title_full Growth regulators alter the development and metabolism of lemon balm seedlings cultured in vitro
title_fullStr Growth regulators alter the development and metabolism of lemon balm seedlings cultured in vitro
title_full_unstemmed Growth regulators alter the development and metabolism of lemon balm seedlings cultured in vitro
title_sort Growth regulators alter the development and metabolism of lemon balm seedlings cultured in vitro
author Silva, Leila Isabel da
author_facet Silva, Leila Isabel da
Magalhães, Hélida Mara
author_role author
author2 Magalhães, Hélida Mara
author2_role author
dc.contributor.author.fl_str_mv Silva, Leila Isabel da
Magalhães, Hélida Mara
dc.subject.por.fl_str_mv Antocianinas. Clorofilas. Lamiaceae. Micropropagação.
Anthocyanins. Chlorophyll. Lamiaceae. Micropropagation.
topic Antocianinas. Clorofilas. Lamiaceae. Micropropagação.
Anthocyanins. Chlorophyll. Lamiaceae. Micropropagation.
description Melissa officinalis L., popularly known as lemon balm, is an aromatic plant widely used in medicine, cosmetics, and pharmaceutical industries for its essential oil rich in phenylpropanoids, terpenes, and phenolics. This study aimed to assess the effect of growth regulators on the development and physiological and biochemical metabolism of M. officinalis cultured in vitro. Seeds were inoculated in Murashige and Skoog medium and added with the regulators 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) according to six different treatments. After 90 days of culture, plants were evaluated for growth and biochemical and physiological parameters (flavonoids, anthocyanins, and chlorophyll). The balance between regulators interfered with plant growth, which increased in the presence of 0.2 mg L−1 BAP. In this treatment, the plants had greater growth with more leaves, and the biomass production of shoots and roots was higher than the control. Growth regulators did not influence nitrogen assimilation or flavonoid production; however, total chlorophyll and anthocyanin indexes were enhanced by treatment with BAP at concentrations ranging from 0.2 to 0.5 mg L−1. Auxin treatment did not improve root production or growth but favored callus formation when combined with 0.5–3.0 mg L−1 BAP. The results indicate that high BAP concentrations (above 1.0 mg L−1) should not be used in in vitro production of lemon balm.
publishDate 2023
dc.date.none.fl_str_mv 2023-02-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufersa.edu.br/caatinga/article/view/11072
10.1590/1983-21252023v36n214rc
url https://periodicos.ufersa.edu.br/caatinga/article/view/11072
identifier_str_mv 10.1590/1983-21252023v36n214rc
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.ufersa.edu.br/caatinga/article/view/11072/11146
dc.rights.driver.fl_str_mv Copyright (c) 2023 Revista Caatinga
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Revista Caatinga
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
publisher.none.fl_str_mv Universidade Federal Rural do Semi-Árido
dc.source.none.fl_str_mv REVISTA CAATINGA; Vol. 36 No. 2 (2023); 372-380
Revista Caatinga; v. 36 n. 2 (2023); 372-380
1983-2125
0100-316X
reponame:Revista Caatinga
instname:Universidade Federal Rural do Semi-Árido (UFERSA)
instacron:UFERSA
instname_str Universidade Federal Rural do Semi-Árido (UFERSA)
instacron_str UFERSA
institution UFERSA
reponame_str Revista Caatinga
collection Revista Caatinga
repository.name.fl_str_mv Revista Caatinga - Universidade Federal Rural do Semi-Árido (UFERSA)
repository.mail.fl_str_mv patricio@ufersa.edu.br|| caatinga@ufersa.edu.br
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