A influência da semente de linhaça como fonte de fitoestrógenos nos indicadores cardiovasculares e na menopausa de ratas castradas
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
Texto Completo: | https://app.uff.br/riuff/handle/1/18433 |
Resumo: | Flaxseed has attracted the interest of the scientific community for presenting innumerable benefits to the health. It was objectified to randomly evaluate the influence of this seed in the cardiovascular pointers and the menopause of 21 Wistar rats that had been submitted to bilateral oophorectomy for induction of surgical menopause, drafted in three groups: Control Group (GC) fed with ration of casein; Flaxseed Group (GL) fed with ration of casein, added of 25% of flaxseed and Modified Control Group (GCM) fed with ration of casein, added of staple fibers and fat. All the rations with 10% of protein were elaborated according to recommendations of AIN 93. The rats were kept in environment with temperature (22ºC ± 2ºC) and adequate illumination (clearly/dark cycle of 12 in 12 hours), receiving ration and water. It was collected every two days the consumption of ration (g) and variation of weight (g) for determination of the alimentary profile of the animals. The vaginal cytology was collected every 30 days, fixed in microscopic slides and stained by the Papanicolaou technique. After 180 days, the rats were anesthetized with thiopental (Tiopentax® - 0.15 ml/100g bw, i.p.), for the collection of the blood by cardiac puncture. It was determined the serum total cholesterol concentrations and fractions (HDL-c, LDL-c and VLDL-c) and triglycerides through LABTEST® s biochemist. Furthermore, the determination of 17β-estradiol was carried through with Diagnostics Products Corporation s kits (DPC®) with radioimunotest. The non-parametric test of Kruskal-Wallis was used. In case of significant differences, the post hoc U test of Mann-Whitney was applied for peer groups comparison through the Sofware S-Plus version 6.0, with the level of p≤0.05. Significant total cholesterol reduction of the GL in relation to the others groups was observed (p=0.0005), as well as an (non-significant) increase of the HDL-c of the same group (p=0.0745), however, it was not observed modification for the data of LDL-c, VLDL-c and triglycerides. The ration consumption was similar between the groups, however the weight variation was different (p=0.0005). Although presenting superior value of 17β-estradiol (p=0.0012) in relation to the others groups, the GL did not demonstrate influence in the vaginal cytology of the rats. The data had demonstrated that the addition of flaxseed to the rations in such a way had a positive influence on the lipidic profile as much as on the alimentary profile of the animals and that although presenting a significant estrogenic action, this was not enough to modify the vaginal cytological profile of the animals. |
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A influência da semente de linhaça como fonte de fitoestrógenos nos indicadores cardiovasculares e na menopausa de ratas castradasSemente de linhaçaMenopausaIndicadores cardiovascularesRatasOoforectomiaPerfil lipídicoPerfil alimentar17β-estradiolCitologia vaginalMEDICINACIÊNCIAS MÉDICASRatos WistarLipídioOvariectomiaFlaxseedRatsOophorectomyLipid profileAlimentary profile17β estradiolVaginal cytologyCNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICAFlaxseed has attracted the interest of the scientific community for presenting innumerable benefits to the health. It was objectified to randomly evaluate the influence of this seed in the cardiovascular pointers and the menopause of 21 Wistar rats that had been submitted to bilateral oophorectomy for induction of surgical menopause, drafted in three groups: Control Group (GC) fed with ration of casein; Flaxseed Group (GL) fed with ration of casein, added of 25% of flaxseed and Modified Control Group (GCM) fed with ration of casein, added of staple fibers and fat. All the rations with 10% of protein were elaborated according to recommendations of AIN 93. The rats were kept in environment with temperature (22ºC ± 2ºC) and adequate illumination (clearly/dark cycle of 12 in 12 hours), receiving ration and water. It was collected every two days the consumption of ration (g) and variation of weight (g) for determination of the alimentary profile of the animals. The vaginal cytology was collected every 30 days, fixed in microscopic slides and stained by the Papanicolaou technique. After 180 days, the rats were anesthetized with thiopental (Tiopentax® - 0.15 ml/100g bw, i.p.), for the collection of the blood by cardiac puncture. It was determined the serum total cholesterol concentrations and fractions (HDL-c, LDL-c and VLDL-c) and triglycerides through LABTEST® s biochemist. Furthermore, the determination of 17β-estradiol was carried through with Diagnostics Products Corporation s kits (DPC®) with radioimunotest. The non-parametric test of Kruskal-Wallis was used. In case of significant differences, the post hoc U test of Mann-Whitney was applied for peer groups comparison through the Sofware S-Plus version 6.0, with the level of p≤0.05. Significant total cholesterol reduction of the GL in relation to the others groups was observed (p=0.0005), as well as an (non-significant) increase of the HDL-c of the same group (p=0.0745), however, it was not observed modification for the data of LDL-c, VLDL-c and triglycerides. The ration consumption was similar between the groups, however the weight variation was different (p=0.0005). Although presenting superior value of 17β-estradiol (p=0.0012) in relation to the others groups, the GL did not demonstrate influence in the vaginal cytology of the rats. The data had demonstrated that the addition of flaxseed to the rations in such a way had a positive influence on the lipidic profile as much as on the alimentary profile of the animals and that although presenting a significant estrogenic action, this was not enough to modify the vaginal cytological profile of the animals.A semente da linhaça tem atraído o interesse da comunidade científica por apresentar inúmeros benefícios à saúde. Objetivou-se avaliar a influência desta semente nos indicadores cardiovasculares e na menopausa de 21 ratas Wistar que foram submetidas à ooforectomia bilateral para indução de uma menopausa cirúrgica, sorteadas aleatoriamente em três grupos: Grupo Controle (GC) com ração de caseína; Grupo Linhaça (GL) com ração de caseína, adicionada de 25% de semente de linhaça e Grupo Controle Modificado (GCM) com ração de caseína, adicionada de fibras e lipídio. Todas as rações com 10% de proteína, elaboradas segundo as recomendações da AIN 93, mantidos em ambiente com temperatura (22ºC ± 2ºC) e iluminação adequada (ciclo claro / escuro de 12 em 12 horas), recebendo ração e água. Coletou-se a cada dois dias dados de consumo de ração (g) e variação de peso (g) para determinação do perfil alimentar dos animais. Mensalmente, foi coletada a citologia vaginal das ratas, cujo material foi fixado em lâminas e corado por técnica Papanicolau para observação posterior. Após 180 dias, foram anestesiadas com tiopental (Tiopentax® - 0,15 ml/100g pc, ip), para a coleta do sangue por punção cardíaca. Determinaram-se as concentrações séricas de colesterol total e frações (HDL-c, LDL-c e VLDL-c) e triglicerídeos através de Kits bioquímicos LABTEST®, além da determinação do 17β-estradiol com utilização de kits Diagnostic Products Corporation (DPC®), por radioimunoensaios. Utilizou-se o teste não paramétrico de Kruskal-Wallis. Em caso de diferenças significativas, aplicou-se o teste post hoc U de Mann-Whitney para comparação dois a dois através do Sofware S-Plus versão 6.0, com o nível de p ≤ 0,05. Observou-se significante redução de colesterol total do GL em relação aos demais (p=0,0005), assim como aumento (não significativo) do HDL-c do mesmo grupo (p=0,0745), porém não houve modificação para os dados de LDL-c, VLDL-c e triglicerídios. O consumo de ração foi semelhante entre os grupos, porém a variação de peso foi diferente (p=0,0005). Apesar de apresentar superior valor de 17β-estradiol (p=0,0012) em relação aos demais grupos, o GL não demonstrou influência na citologia vaginal das ratas. Os dados demonstraram que a adição da semente de linhaça às rações influenciou de forma positiva tanto o perfil lipídico quanto o perfil alimentar dos animais e que apesar de apresentar uma significante ação estrogênica, essa não foi suficiente para causar uma modificação no perfil citológico vaginal dos animais.Programa de Pós-graduação em Ciências MédicasCiências MédicasBoaventura, Gilson TelesCPF:27152618122http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793697U7Silva, Maria Angélica GuzmánCPF:46826718715http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783592U2Cardoso, Gilberto PerezCPF:95234953622http://lattes.cnpq.br/3949041976687209Ramos, Cristiane da FonteCPF:28482871822http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785662E9Mesquita, Eliana de Fatima Marques deCPF:45120098722http://lattes.cnpq.br/4109356329417202Velarde, Luis Guillermo CocaCPF:07968181922http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792349Y0Martucci, Renata BrumCPF:89681719322http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4769633E6Pacheco, Juliana Tomaz2021-03-10T20:44:33Z2009-06-152021-03-10T20:44:33Z2006-10-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://app.uff.br/riuff/handle/1/18433porCC-BY-SAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF)instname:Universidade Federal Fluminense (UFF)instacron:UFF2021-03-10T20:44:33Zoai:app.uff.br:1/18433Repositório InstitucionalPUBhttps://app.uff.br/oai/requestriuff@id.uff.bropendoar:21202021-03-10T20:44:33Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF)false |
dc.title.none.fl_str_mv |
A influência da semente de linhaça como fonte de fitoestrógenos nos indicadores cardiovasculares e na menopausa de ratas castradas |
title |
A influência da semente de linhaça como fonte de fitoestrógenos nos indicadores cardiovasculares e na menopausa de ratas castradas |
spellingShingle |
A influência da semente de linhaça como fonte de fitoestrógenos nos indicadores cardiovasculares e na menopausa de ratas castradas Pacheco, Juliana Tomaz Semente de linhaça Menopausa Indicadores cardiovasculares Ratas Ooforectomia Perfil lipídico Perfil alimentar 17β -estradiol Citologia vaginal MEDICINA CIÊNCIAS MÉDICAS Ratos Wistar Lipídio Ovariectomia Flaxseed Rats Oophorectomy Lipid profile Alimentary profile 17β estradiol Vaginal cytology CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA |
title_short |
A influência da semente de linhaça como fonte de fitoestrógenos nos indicadores cardiovasculares e na menopausa de ratas castradas |
title_full |
A influência da semente de linhaça como fonte de fitoestrógenos nos indicadores cardiovasculares e na menopausa de ratas castradas |
title_fullStr |
A influência da semente de linhaça como fonte de fitoestrógenos nos indicadores cardiovasculares e na menopausa de ratas castradas |
title_full_unstemmed |
A influência da semente de linhaça como fonte de fitoestrógenos nos indicadores cardiovasculares e na menopausa de ratas castradas |
title_sort |
A influência da semente de linhaça como fonte de fitoestrógenos nos indicadores cardiovasculares e na menopausa de ratas castradas |
author |
Pacheco, Juliana Tomaz |
author_facet |
Pacheco, Juliana Tomaz |
author_role |
author |
dc.contributor.none.fl_str_mv |
Boaventura, Gilson Teles CPF:27152618122 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793697U7 Silva, Maria Angélica Guzmán CPF:46826718715 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783592U2 Cardoso, Gilberto Perez CPF:95234953622 http://lattes.cnpq.br/3949041976687209 Ramos, Cristiane da Fonte CPF:28482871822 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4785662E9 Mesquita, Eliana de Fatima Marques de CPF:45120098722 http://lattes.cnpq.br/4109356329417202 Velarde, Luis Guillermo Coca CPF:07968181922 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792349Y0 Martucci, Renata Brum CPF:89681719322 http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4769633E6 |
dc.contributor.author.fl_str_mv |
Pacheco, Juliana Tomaz |
dc.subject.por.fl_str_mv |
Semente de linhaça Menopausa Indicadores cardiovasculares Ratas Ooforectomia Perfil lipídico Perfil alimentar 17β -estradiol Citologia vaginal MEDICINA CIÊNCIAS MÉDICAS Ratos Wistar Lipídio Ovariectomia Flaxseed Rats Oophorectomy Lipid profile Alimentary profile 17β estradiol Vaginal cytology CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA |
topic |
Semente de linhaça Menopausa Indicadores cardiovasculares Ratas Ooforectomia Perfil lipídico Perfil alimentar 17β -estradiol Citologia vaginal MEDICINA CIÊNCIAS MÉDICAS Ratos Wistar Lipídio Ovariectomia Flaxseed Rats Oophorectomy Lipid profile Alimentary profile 17β estradiol Vaginal cytology CNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA |
description |
Flaxseed has attracted the interest of the scientific community for presenting innumerable benefits to the health. It was objectified to randomly evaluate the influence of this seed in the cardiovascular pointers and the menopause of 21 Wistar rats that had been submitted to bilateral oophorectomy for induction of surgical menopause, drafted in three groups: Control Group (GC) fed with ration of casein; Flaxseed Group (GL) fed with ration of casein, added of 25% of flaxseed and Modified Control Group (GCM) fed with ration of casein, added of staple fibers and fat. All the rations with 10% of protein were elaborated according to recommendations of AIN 93. The rats were kept in environment with temperature (22ºC ± 2ºC) and adequate illumination (clearly/dark cycle of 12 in 12 hours), receiving ration and water. It was collected every two days the consumption of ration (g) and variation of weight (g) for determination of the alimentary profile of the animals. The vaginal cytology was collected every 30 days, fixed in microscopic slides and stained by the Papanicolaou technique. After 180 days, the rats were anesthetized with thiopental (Tiopentax® - 0.15 ml/100g bw, i.p.), for the collection of the blood by cardiac puncture. It was determined the serum total cholesterol concentrations and fractions (HDL-c, LDL-c and VLDL-c) and triglycerides through LABTEST® s biochemist. Furthermore, the determination of 17β-estradiol was carried through with Diagnostics Products Corporation s kits (DPC®) with radioimunotest. The non-parametric test of Kruskal-Wallis was used. In case of significant differences, the post hoc U test of Mann-Whitney was applied for peer groups comparison through the Sofware S-Plus version 6.0, with the level of p≤0.05. Significant total cholesterol reduction of the GL in relation to the others groups was observed (p=0.0005), as well as an (non-significant) increase of the HDL-c of the same group (p=0.0745), however, it was not observed modification for the data of LDL-c, VLDL-c and triglycerides. The ration consumption was similar between the groups, however the weight variation was different (p=0.0005). Although presenting superior value of 17β-estradiol (p=0.0012) in relation to the others groups, the GL did not demonstrate influence in the vaginal cytology of the rats. The data had demonstrated that the addition of flaxseed to the rations in such a way had a positive influence on the lipidic profile as much as on the alimentary profile of the animals and that although presenting a significant estrogenic action, this was not enough to modify the vaginal cytological profile of the animals. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-11 2009-06-15 2021-03-10T20:44:33Z 2021-03-10T20:44:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://app.uff.br/riuff/handle/1/18433 |
url |
https://app.uff.br/riuff/handle/1/18433 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
CC-BY-SA info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
CC-BY-SA |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Programa de Pós-graduação em Ciências Médicas Ciências Médicas |
publisher.none.fl_str_mv |
Programa de Pós-graduação em Ciências Médicas Ciências Médicas |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Federal Fluminense (RIUFF) instname:Universidade Federal Fluminense (UFF) instacron:UFF |
instname_str |
Universidade Federal Fluminense (UFF) |
instacron_str |
UFF |
institution |
UFF |
reponame_str |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
collection |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Federal Fluminense (RIUFF) - Universidade Federal Fluminense (UFF) |
repository.mail.fl_str_mv |
riuff@id.uff.br |
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1807838790687916032 |