Mastite subclínica: patógenos isolados e respectiva sensibilidade antimicrobiana, variação da contagem de células somáticas e fatores de risco

• Autor(a) principal: COSTA, Anna Carolina da
• Data de Publicação: 2010
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFG
• Texto Completo: http://repositorio.bc.ufg.br/tede/handle/tde/942
• topic: fitoterapia; leite; patógenos; resistência antimicrobiana; 1. Fitoterapia 2. Leite 3. Patógenos 4. Resistência Antimicrobiana; herbal medicine; milk; pathogens; antimicrobial resistance; CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS

This study was conducted on 12 dairy farms and in laboratories Bacteriology and Quality of Milk, both of the School of Veterinary Medicine Federal University of Goias objective of this study to characterize the microorganisms in milk from cows with subclinical mastitis and relate the pathogens identified with the variation of cell count somatic (CCS), also identify the risk factors and the likely sources of infection to subclinical mastitis, by visual observations, application of questionnaires, by isolation and identification of pathogens from the hands of milkers, swabs and solutions liners and pre posdipping. The profile sensitivity to key antimicrobial and bactericidal effect of the extract of Calendula officinalis has been determined on the pathogens isolated in most cases subclinical mastitis in herds. To evaluate the variation of CCS in relation to the pathogens involved, we used analysis of frequency and Chi-square, and the risk factors were analyzed for Logistic regression to test associations between variables and increased CCS. It was found that agents with higher frequency of isolation were S. aureus (28.8% of samples), E. coli (19.8%) and Enterobacter spp. (11.3%). CCS average herd was approximately 875 x 103cél/mL, and the type etiologic agent of a significant influence on the variation in SCC. It was found that S. aureus and Streptococcus spp. were responsible for greater increase in SCC, with an average of 1192 x 1174 x 103cél/mL and 103cél/mL, respectively. This variation was significantly higher (p <0.05) when compared to the average SCC in milk were the other isolates microorganisms: S. coagulase negative, Pseudomonas spp., E. coli and Enterobacter spp. It was found that the risk factors that showed significant association with increased SCC were unsatisfactory hygiene environment and milker, inadequate drying of the teats, and factors related to milking equipment, such as poor maintenance and inadequate cleaning. The hands of the milkers and the sets of liners were able to convey both the infectious agents as the environment, important in epidemiology of bovine mastitis. It was also concluded that the extract of marigold showed bactericidal activity in vitro against S. aureus isolates, and antimicrobial agents used showed variation in the spectrum of sensitivity.