Comparison of Leifsonia xyli subsp. xyli molecular detection in heat-treated sugarcane setts
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Agropecuária Tropical (Online) |
Texto Completo: | https://revistas.ufg.br/pat/article/view/55132 |
Resumo: | The thermotherapy of sugarcane setts is currently the primary management method for Leifsonia xyli subsp. xyli (Lxx), in Brazil. When they are immersed, the enzymes and proteins of the bacterial cell are denatured without harming the setts buds. Due to possible escapes from detection and consequent bacterium survival to thermotherapy, what may result in asymptomatic seedlings, this study aimed to detect the Leifsonia xyli subsp. xyli bacterium in sugarcane setts using molecular techniques and different time and temperature combinations, with or without the addition of antibiotics. The conventional PCR method detected the Lxx bacterial DNA only in the positive control, consisting of a highly susceptible plant with a high bacterial concentration. Using the nested-PCR, the Lxx DNA was detected in all the treatments used. Thus, none of the treatments adopted in the thermotherapy was able to eliminate the Lxx from the setts, and the use of kasugamicin also did not eliminate the bacterium, but reduced the bacterial population in the tested treatments. These results confirm that the nested-PCR is a useful tool to detect the presence of this phytobacterium in setts that will be used as seedlings. KEYWORDS: Heat treatment, ratoon stunting disease, polimerase chain reaction. |
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Comparison of Leifsonia xyli subsp. xyli molecular detection in heat-treated sugarcane settsComparação de detecção molecular de Leifsonia xyli subsp. xyli em toletes de cana-de-açúcar tratados termicamentecontrolratoon stunting diseasenested PCRThe thermotherapy of sugarcane setts is currently the primary management method for Leifsonia xyli subsp. xyli (Lxx), in Brazil. When they are immersed, the enzymes and proteins of the bacterial cell are denatured without harming the setts buds. Due to possible escapes from detection and consequent bacterium survival to thermotherapy, what may result in asymptomatic seedlings, this study aimed to detect the Leifsonia xyli subsp. xyli bacterium in sugarcane setts using molecular techniques and different time and temperature combinations, with or without the addition of antibiotics. The conventional PCR method detected the Lxx bacterial DNA only in the positive control, consisting of a highly susceptible plant with a high bacterial concentration. Using the nested-PCR, the Lxx DNA was detected in all the treatments used. Thus, none of the treatments adopted in the thermotherapy was able to eliminate the Lxx from the setts, and the use of kasugamicin also did not eliminate the bacterium, but reduced the bacterial population in the tested treatments. These results confirm that the nested-PCR is a useful tool to detect the presence of this phytobacterium in setts that will be used as seedlings. KEYWORDS: Heat treatment, ratoon stunting disease, polimerase chain reaction.O tratamento térmico de toletes para a formação de mudas de cana-de-açúcar é a principal forma de manejo de Leifsonia xyli subsp. xyli (Lxx), atualmente, no Brasil. Quando imersos, as enzimas e proteínas da célula bacteriana são desnaturadas, sem causar prejuízos às gemas dos toletes. Devido a possíveis escapes à detecção e consequente sobrevivência da bactéria à termoterapia, o que pode dar origem a mudas assintomáticas, objetivou-se realizar a detecção da bactéria Leifsonia xyli subsp. xyli em toletes de cana-de-açúcar, utilizando-se técnicas moleculares e combinações de tempo e temperatura, com ou sem a adição de antibióticos. A técnica PCR convencional detectou o DNA bacteriano de Lxx somente no controle positivo, constituído de planta altamente suscetível, com alta concentração bacteriana. Já pela nested-PCR, o DNA de Lxx foi detectado em todos os tratamentos utilizados. Assim, nenhum dos tratamentos adotados na termoterapia foi capaz de eliminar a Lxx dos toletes, bem como o uso de kasugamicina também não propiciou a eliminação da bactéria, mas reduziu sua população nos tratamentos testados. Estes resultados atestam a nested-PCR como ferramenta útil para a comprovação da presença da fitobactéria em toletes que serão utilizados como mudas. PALAVRAS-CHAVE: Termoterapia, raquitismo-das-soqueiras, reação em cadeia da polimerase.Escola de Agronomia - Universidade Federal de Goiás2019-07-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/pat/article/view/55132Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 49 (2019); e55132Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 49 (2019); e55132Pesquisa Agropecuária Tropical; v. 49 (2019); e551321983-4063reponame:Pesquisa Agropecuária Tropical (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGenghttps://revistas.ufg.br/pat/article/view/55132/33350Dias, Vanessa DuarteCarrer Filho, RenatoCunha, Marcos Gomes dainfo:eu-repo/semantics/openAccess2020-03-02T15:02:12Zoai:ojs.revistas.ufg.br:article/55132Revistahttps://revistas.ufg.br/patPUBhttps://revistas.ufg.br/pat/oaiaseleguini.pat@gmail.com||mgoes@agro.ufg.br1983-40631517-6398opendoar:2024-05-21T19:56:24.813833Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
Comparison of Leifsonia xyli subsp. xyli molecular detection in heat-treated sugarcane setts Comparação de detecção molecular de Leifsonia xyli subsp. xyli em toletes de cana-de-açúcar tratados termicamente |
title |
Comparison of Leifsonia xyli subsp. xyli molecular detection in heat-treated sugarcane setts |
spellingShingle |
Comparison of Leifsonia xyli subsp. xyli molecular detection in heat-treated sugarcane setts Dias, Vanessa Duarte control ratoon stunting disease nested PCR |
title_short |
Comparison of Leifsonia xyli subsp. xyli molecular detection in heat-treated sugarcane setts |
title_full |
Comparison of Leifsonia xyli subsp. xyli molecular detection in heat-treated sugarcane setts |
title_fullStr |
Comparison of Leifsonia xyli subsp. xyli molecular detection in heat-treated sugarcane setts |
title_full_unstemmed |
Comparison of Leifsonia xyli subsp. xyli molecular detection in heat-treated sugarcane setts |
title_sort |
Comparison of Leifsonia xyli subsp. xyli molecular detection in heat-treated sugarcane setts |
author |
Dias, Vanessa Duarte |
author_facet |
Dias, Vanessa Duarte Carrer Filho, Renato Cunha, Marcos Gomes da |
author_role |
author |
author2 |
Carrer Filho, Renato Cunha, Marcos Gomes da |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Dias, Vanessa Duarte Carrer Filho, Renato Cunha, Marcos Gomes da |
dc.subject.por.fl_str_mv |
control ratoon stunting disease nested PCR |
topic |
control ratoon stunting disease nested PCR |
description |
The thermotherapy of sugarcane setts is currently the primary management method for Leifsonia xyli subsp. xyli (Lxx), in Brazil. When they are immersed, the enzymes and proteins of the bacterial cell are denatured without harming the setts buds. Due to possible escapes from detection and consequent bacterium survival to thermotherapy, what may result in asymptomatic seedlings, this study aimed to detect the Leifsonia xyli subsp. xyli bacterium in sugarcane setts using molecular techniques and different time and temperature combinations, with or without the addition of antibiotics. The conventional PCR method detected the Lxx bacterial DNA only in the positive control, consisting of a highly susceptible plant with a high bacterial concentration. Using the nested-PCR, the Lxx DNA was detected in all the treatments used. Thus, none of the treatments adopted in the thermotherapy was able to eliminate the Lxx from the setts, and the use of kasugamicin also did not eliminate the bacterium, but reduced the bacterial population in the tested treatments. These results confirm that the nested-PCR is a useful tool to detect the presence of this phytobacterium in setts that will be used as seedlings. KEYWORDS: Heat treatment, ratoon stunting disease, polimerase chain reaction. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/pat/article/view/55132 |
url |
https://revistas.ufg.br/pat/article/view/55132 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/pat/article/view/55132/33350 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Escola de Agronomia - Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Pesquisa Agropecuária Tropical [Agricultural Research in the Tropics]; Vol. 49 (2019); e55132 Pesquisa Agropecuária Tropical (Agricultural Research in the Tropics); Vol. 49 (2019); e55132 Pesquisa Agropecuária Tropical; v. 49 (2019); e55132 1983-4063 reponame:Pesquisa Agropecuária Tropical (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Pesquisa Agropecuária Tropical (Online) |
collection |
Pesquisa Agropecuária Tropical (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Tropical (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
aseleguini.pat@gmail.com||mgoes@agro.ufg.br |
_version_ |
1799874820425383936 |