INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Ciência animal brasileira (Online) |
DOI: | 10.5216/cab.v9i3.1566 |
Texto Completo: | https://revistas.ufg.br/vet/article/view/1566 |
Resumo: | Insulin is present on seminal plasma and it can be involved on sperm capacitation and sperm autocrine metabolism (AQUILA et al., 2005). It was verified the effect of insulin addition on ovine frozen semen extender. The parameters analyzed were sperm motility, sperm cinematic, acrossoma integrity and membrane functionality. Insulin was added to fraction B of TRIS-yolk extender in different concentrations. Sperm motility and cinematic were evaluated 0, 3 and 6 hours after thawing while acrossoma integrity and membrane functionality were evaluated 0 and 6 hours after thawing. The addition of 3 UI/mL of insulin increased amplitude lateral of head (5.42±0.97; C:4.75±0.47) and acrossoma integrity (153.67±16.98; C:134.00±18.08). The addition of 0.3 UI/mL of insulin improved retilinearity tree hours after thawing (83.17±2.71; C:78.67±4.97). It was verified a increase on linearity values on treatments with 0.3 e 3 UI/mL (52.17±2.14; 51.67±3.14; C:46.83±3.60), respectively tree hours after thawing. Treatment with 0.3 e 3 UI/ml showed higher acrossoma integrity than control (146.17±10.91; 146.33±16.02; C:117.00±17.80). Addiction of insulin on ovine frozen semen extender improved sperm motility, sperm cinematic and acrossoma integrity.KEY WORDS: Freezing, insulin, ovine, spermetozoa. |
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Ciência animal brasileira (Online) |
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INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMENNFLUÊNCIA DA INSULINA NA CONGELABILIDADE DO SÊMEN DE OVINOInsulin is present on seminal plasma and it can be involved on sperm capacitation and sperm autocrine metabolism (AQUILA et al., 2005). It was verified the effect of insulin addition on ovine frozen semen extender. The parameters analyzed were sperm motility, sperm cinematic, acrossoma integrity and membrane functionality. Insulin was added to fraction B of TRIS-yolk extender in different concentrations. Sperm motility and cinematic were evaluated 0, 3 and 6 hours after thawing while acrossoma integrity and membrane functionality were evaluated 0 and 6 hours after thawing. The addition of 3 UI/mL of insulin increased amplitude lateral of head (5.42±0.97; C:4.75±0.47) and acrossoma integrity (153.67±16.98; C:134.00±18.08). The addition of 0.3 UI/mL of insulin improved retilinearity tree hours after thawing (83.17±2.71; C:78.67±4.97). It was verified a increase on linearity values on treatments with 0.3 e 3 UI/mL (52.17±2.14; 51.67±3.14; C:46.83±3.60), respectively tree hours after thawing. Treatment with 0.3 e 3 UI/ml showed higher acrossoma integrity than control (146.17±10.91; 146.33±16.02; C:117.00±17.80). Addiction of insulin on ovine frozen semen extender improved sperm motility, sperm cinematic and acrossoma integrity.KEY WORDS: Freezing, insulin, ovine, spermetozoa.A insulina está presente no plasma seminal e pode envolver-se em eventos da capacitação e metabolismo autócrino do espermatozóide (AQUILA et al., 2005). Assim, estudaram-se os efeitos da insulina adicionada ao diluente de congelamento para sêmen e sua conseqüência na criopreservação, integridade de acrossoma e funcionalidade de membrana de espermatozóide ovino. A insulina foi adicionada na fração B do diluente TRIS-gema em diferentes concentrações. As avaliações da motilidade e cinemática realizaram-se às 0, 3 e 6 horas, enquanto a funcionalidade e integridade de membrana foram avaliadas às 0 e 6 horas após o descongelamento. A adição de 3,0 UI/mL de insulina aumentou (P>0,05) a amplitude lateral de cabeça (5,42±0,97; C:4,75±0,47) e acrossomas íntegros (153,67±16,98; C:134,00±18,08). A concentração de 0,3 UI/mL de insulina melhorou a retilinearidade, quando verificada no período de 3 horas (83,17±2,71; C:78,67±4,97). Nesse mesmo período, observou-se uma elevação nos valores de linearidade nas concentrações de 0,3 e 3,0 UI/mL de insulina (52,17±2,14; 51,67±3,14; C:46,83±3,60, respectivamente). Seis horas após o descongelamento, os tratamentos com 0,3 e 3,0 UI/mL apresentaram valores de acrossomas íntegros mais elevados (146,17±10,91; 146,33±16,02; C:117,00±17,80, respectivamente). A adição de insulina ao diluente aumentou a motilidade, cinemática e integridade de acrossoma do sêmen ovino congelado.PALAVRAS-CHAVES: Congelamento, espermatozóides, insulina, ovino.Universidade Federal de Goiás2008-10-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/vet/article/view/156610.5216/cab.v9i3.1566Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 9 No. 3 (2008); 731-739Ciência Animal Brasileira / Brazilian Animal Science; v. 9 n. 3 (2008); 731-7391809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/1566/4160van Tilburg, Mauricio FragaSilva, José Frederico StraggiottiDias, Angelo José BurlaQuirino, Célia RaquelFagundes, Brunoinfo:eu-repo/semantics/openAccess2008-10-21T13:02:11Zoai:ojs.revistas.ufg.br:article/1566Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:54:56.446753Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true |
dc.title.none.fl_str_mv |
INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN NFLUÊNCIA DA INSULINA NA CONGELABILIDADE DO SÊMEN DE OVINO |
title |
INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN |
spellingShingle |
INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN van Tilburg, Mauricio Fraga van Tilburg, Mauricio Fraga |
title_short |
INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN |
title_full |
INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN |
title_fullStr |
INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN |
title_full_unstemmed |
INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN |
title_sort |
INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN |
author |
van Tilburg, Mauricio Fraga |
author_facet |
van Tilburg, Mauricio Fraga van Tilburg, Mauricio Fraga Silva, José Frederico Straggiotti Dias, Angelo José Burla Quirino, Célia Raquel Fagundes, Bruno Silva, José Frederico Straggiotti Dias, Angelo José Burla Quirino, Célia Raquel Fagundes, Bruno |
author_role |
author |
author2 |
Silva, José Frederico Straggiotti Dias, Angelo José Burla Quirino, Célia Raquel Fagundes, Bruno |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
van Tilburg, Mauricio Fraga Silva, José Frederico Straggiotti Dias, Angelo José Burla Quirino, Célia Raquel Fagundes, Bruno |
description |
Insulin is present on seminal plasma and it can be involved on sperm capacitation and sperm autocrine metabolism (AQUILA et al., 2005). It was verified the effect of insulin addition on ovine frozen semen extender. The parameters analyzed were sperm motility, sperm cinematic, acrossoma integrity and membrane functionality. Insulin was added to fraction B of TRIS-yolk extender in different concentrations. Sperm motility and cinematic were evaluated 0, 3 and 6 hours after thawing while acrossoma integrity and membrane functionality were evaluated 0 and 6 hours after thawing. The addition of 3 UI/mL of insulin increased amplitude lateral of head (5.42±0.97; C:4.75±0.47) and acrossoma integrity (153.67±16.98; C:134.00±18.08). The addition of 0.3 UI/mL of insulin improved retilinearity tree hours after thawing (83.17±2.71; C:78.67±4.97). It was verified a increase on linearity values on treatments with 0.3 e 3 UI/mL (52.17±2.14; 51.67±3.14; C:46.83±3.60), respectively tree hours after thawing. Treatment with 0.3 e 3 UI/ml showed higher acrossoma integrity than control (146.17±10.91; 146.33±16.02; C:117.00±17.80). Addiction of insulin on ovine frozen semen extender improved sperm motility, sperm cinematic and acrossoma integrity.KEY WORDS: Freezing, insulin, ovine, spermetozoa. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-10-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistas.ufg.br/vet/article/view/1566 10.5216/cab.v9i3.1566 |
url |
https://revistas.ufg.br/vet/article/view/1566 |
identifier_str_mv |
10.5216/cab.v9i3.1566 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistas.ufg.br/vet/article/view/1566/4160 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Goiás |
publisher.none.fl_str_mv |
Universidade Federal de Goiás |
dc.source.none.fl_str_mv |
Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 9 No. 3 (2008); 731-739 Ciência Animal Brasileira / Brazilian Animal Science; v. 9 n. 3 (2008); 731-739 1809-6891 1518-2797 reponame:Ciência animal brasileira (Online) instname:Universidade Federal de Goiás (UFG) instacron:UFG |
instname_str |
Universidade Federal de Goiás (UFG) |
instacron_str |
UFG |
institution |
UFG |
reponame_str |
Ciência animal brasileira (Online) |
collection |
Ciência animal brasileira (Online) |
repository.name.fl_str_mv |
Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG) |
repository.mail.fl_str_mv |
||revistacab@gmail.com |
_version_ |
1822181512410300416 |
dc.identifier.doi.none.fl_str_mv |
10.5216/cab.v9i3.1566 |