INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN

Detalhes bibliográficos
Autor(a) principal: van Tilburg, Mauricio Fraga
Data de Publicação: 2008
Outros Autores: Silva, José Frederico Straggiotti, Dias, Angelo José Burla, Quirino, Célia Raquel, Fagundes, Bruno
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
DOI: 10.5216/cab.v9i3.1566
Texto Completo: https://revistas.ufg.br/vet/article/view/1566
Resumo: Insulin is present on seminal plasma and it can be involved on sperm capacitation and sperm autocrine metabolism (AQUILA et al., 2005). It was verified the effect of insulin addition on ovine frozen semen extender. The parameters analyzed were sperm motility, sperm cinematic, acrossoma integrity and membrane functionality. Insulin was added to fraction B of TRIS-yolk extender in different concentrations. Sperm motility and cinematic were evaluated 0, 3 and 6 hours after thawing while acrossoma integrity and membrane functionality were evaluated 0 and 6 hours after thawing. The addition of 3 UI/mL of insulin increased amplitude lateral of head (5.42±0.97; C:4.75±0.47) and acrossoma integrity (153.67±16.98; C:134.00±18.08). The addition of 0.3 UI/mL of insulin improved retilinearity tree hours after thawing (83.17±2.71; C:78.67±4.97). It was verified a increase on linearity values on treatments with 0.3 e 3 UI/mL (52.17±2.14; 51.67±3.14; C:46.83±3.60), respectively tree hours after thawing. Treatment with 0.3 e 3 UI/ml showed higher acrossoma integrity than control (146.17±10.91; 146.33±16.02; C:117.00±17.80). Addiction of insulin on ovine frozen semen extender improved sperm motility, sperm cinematic and acrossoma integrity.KEY WORDS: Freezing, insulin, ovine, spermetozoa.
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spelling INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMENNFLUÊNCIA DA INSULINA NA CONGELABILIDADE DO SÊMEN DE OVINOInsulin is present on seminal plasma and it can be involved on sperm capacitation and sperm autocrine metabolism (AQUILA et al., 2005). It was verified the effect of insulin addition on ovine frozen semen extender. The parameters analyzed were sperm motility, sperm cinematic, acrossoma integrity and membrane functionality. Insulin was added to fraction B of TRIS-yolk extender in different concentrations. Sperm motility and cinematic were evaluated 0, 3 and 6 hours after thawing while acrossoma integrity and membrane functionality were evaluated 0 and 6 hours after thawing. The addition of 3 UI/mL of insulin increased amplitude lateral of head (5.42±0.97; C:4.75±0.47) and acrossoma integrity (153.67±16.98; C:134.00±18.08). The addition of 0.3 UI/mL of insulin improved retilinearity tree hours after thawing (83.17±2.71; C:78.67±4.97). It was verified a increase on linearity values on treatments with 0.3 e 3 UI/mL (52.17±2.14; 51.67±3.14; C:46.83±3.60), respectively tree hours after thawing. Treatment with 0.3 e 3 UI/ml showed higher acrossoma integrity than control (146.17±10.91; 146.33±16.02; C:117.00±17.80). Addiction of insulin on ovine frozen semen extender improved sperm motility, sperm cinematic and acrossoma integrity.KEY WORDS: Freezing, insulin, ovine, spermetozoa.A insulina está presente no plasma seminal e pode envolver-se em eventos da capacitação e metabolismo autócrino do espermatozóide (AQUILA et al., 2005). Assim, estudaram-se os efeitos da insulina adicionada ao diluente de congelamento para sêmen e sua conseqüência na criopreservação, integridade de acrossoma e funcionalidade de membrana de espermatozóide ovino. A insulina foi adicionada na fração B do diluente TRIS-gema em diferentes concentrações. As avaliações da motilidade e cinemática realizaram-se às 0, 3 e 6 horas, enquanto a funcionalidade e integridade de membrana foram avaliadas às 0 e 6 horas após o descongelamento. A adição de 3,0 UI/mL de insulina aumentou (P>0,05) a amplitude lateral de cabeça (5,42±0,97; C:4,75±0,47) e acrossomas íntegros (153,67±16,98; C:134,00±18,08). A concentração de 0,3 UI/mL de insulina melhorou a retilinearidade, quando verificada no período de 3 horas (83,17±2,71; C:78,67±4,97). Nesse mesmo período, observou-se uma elevação nos valores de linearidade nas concentrações de 0,3 e 3,0 UI/mL de insulina (52,17±2,14; 51,67±3,14; C:46,83±3,60, respectivamente). Seis horas após o descongelamento, os tratamentos com 0,3 e 3,0 UI/mL apresentaram valores de acrossomas íntegros mais elevados (146,17±10,91; 146,33±16,02; C:117,00±17,80, respectivamente). A adição de insulina ao diluente aumentou a motilidade, cinemática e integridade de acrossoma do sêmen ovino congelado.PALAVRAS-CHAVES: Congelamento, espermatozóides, insulina, ovino.Universidade Federal de Goiás2008-10-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://revistas.ufg.br/vet/article/view/156610.5216/cab.v9i3.1566Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 9 No. 3 (2008); 731-739Ciência Animal Brasileira / Brazilian Animal Science; v. 9 n. 3 (2008); 731-7391809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/1566/4160van Tilburg, Mauricio FragaSilva, José Frederico StraggiottiDias, Angelo José BurlaQuirino, Célia RaquelFagundes, Brunoinfo:eu-repo/semantics/openAccess2008-10-21T13:02:11Zoai:ojs.revistas.ufg.br:article/1566Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:54:56.446753Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN
NFLUÊNCIA DA INSULINA NA CONGELABILIDADE DO SÊMEN DE OVINO
title INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN
spellingShingle INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN
INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN
van Tilburg, Mauricio Fraga
van Tilburg, Mauricio Fraga
title_short INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN
title_full INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN
title_fullStr INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN
INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN
title_full_unstemmed INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN
INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN
title_sort INFLUENCE OF INSULIN IN THE FREEZING OF THE OVINE SEMEN
author van Tilburg, Mauricio Fraga
author_facet van Tilburg, Mauricio Fraga
van Tilburg, Mauricio Fraga
Silva, José Frederico Straggiotti
Dias, Angelo José Burla
Quirino, Célia Raquel
Fagundes, Bruno
Silva, José Frederico Straggiotti
Dias, Angelo José Burla
Quirino, Célia Raquel
Fagundes, Bruno
author_role author
author2 Silva, José Frederico Straggiotti
Dias, Angelo José Burla
Quirino, Célia Raquel
Fagundes, Bruno
author2_role author
author
author
author
dc.contributor.author.fl_str_mv van Tilburg, Mauricio Fraga
Silva, José Frederico Straggiotti
Dias, Angelo José Burla
Quirino, Célia Raquel
Fagundes, Bruno
description Insulin is present on seminal plasma and it can be involved on sperm capacitation and sperm autocrine metabolism (AQUILA et al., 2005). It was verified the effect of insulin addition on ovine frozen semen extender. The parameters analyzed were sperm motility, sperm cinematic, acrossoma integrity and membrane functionality. Insulin was added to fraction B of TRIS-yolk extender in different concentrations. Sperm motility and cinematic were evaluated 0, 3 and 6 hours after thawing while acrossoma integrity and membrane functionality were evaluated 0 and 6 hours after thawing. The addition of 3 UI/mL of insulin increased amplitude lateral of head (5.42±0.97; C:4.75±0.47) and acrossoma integrity (153.67±16.98; C:134.00±18.08). The addition of 0.3 UI/mL of insulin improved retilinearity tree hours after thawing (83.17±2.71; C:78.67±4.97). It was verified a increase on linearity values on treatments with 0.3 e 3 UI/mL (52.17±2.14; 51.67±3.14; C:46.83±3.60), respectively tree hours after thawing. Treatment with 0.3 e 3 UI/ml showed higher acrossoma integrity than control (146.17±10.91; 146.33±16.02; C:117.00±17.80). Addiction of insulin on ovine frozen semen extender improved sperm motility, sperm cinematic and acrossoma integrity.KEY WORDS: Freezing, insulin, ovine, spermetozoa.
publishDate 2008
dc.date.none.fl_str_mv 2008-10-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/1566
10.5216/cab.v9i3.1566
url https://revistas.ufg.br/vet/article/view/1566
identifier_str_mv 10.5216/cab.v9i3.1566
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/1566/4160
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 9 No. 3 (2008); 731-739
Ciência Animal Brasileira / Brazilian Animal Science; v. 9 n. 3 (2008); 731-739
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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dc.identifier.doi.none.fl_str_mv 10.5216/cab.v9i3.1566