Freezing equine semen after 24 hours of cooling

Detalhes bibliográficos
Autor(a) principal: Coelho, Rogéria Werner de Almeida
Data de Publicação: 2021
Outros Autores: Dias, Julio Cesar Oliveira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/9939
Resumo: The objective of this work is to bring about technically the equine sperm cooling and freezing method after 24 h refrigeration with four commercial diluents. The technical approach ranges from the tests that precede and prove the viability of the stallion for collection to the thawing of samples. The collection is performed by means of artificial model vagina for horses and animal dummy. After collecting and analyzing the macroscopic (color, appearance, odor and volume) and microscopic (total motility, vigor and sperm concentration) characteristics of fresh semen, seminal samples will be cooled at 5 ºC for 24 h. commercial diluents. After this period, the samples will be centrifuged and the pellet will be resuspended with commercial freezing diluent and cryopreserved at - 196 ºC in straws. The blades will be thawed at 37 ° C for use in artificial insemination.
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spelling Freezing equine semen after 24 hours of coolingCongelación de semen equino después de 24 horas de enfriamientoCongelação de sêmen equino após 24 horas de resfriamentoInseminación artificialEnfriamiento y congelación de semenViabilidad del semental.Artificial inseminationSemen cooling and freezingStallion viability.Inseminação artificialResfriamento e congelamento de sêmenViabilidade do garanhão.The objective of this work is to bring about technically the equine sperm cooling and freezing method after 24 h refrigeration with four commercial diluents. The technical approach ranges from the tests that precede and prove the viability of the stallion for collection to the thawing of samples. The collection is performed by means of artificial model vagina for horses and animal dummy. After collecting and analyzing the macroscopic (color, appearance, odor and volume) and microscopic (total motility, vigor and sperm concentration) characteristics of fresh semen, seminal samples will be cooled at 5 ºC for 24 h. commercial diluents. After this period, the samples will be centrifuged and the pellet will be resuspended with commercial freezing diluent and cryopreserved at - 196 ºC in straws. The blades will be thawed at 37 ° C for use in artificial insemination.El objetivo de este trabajo es acercar de forma técnica el método de enfriamiento y congelación de espermatozoides de la especie equina después de refrigeración durante 24 h con cuatro diluyentes comerciales. El enfoque técnico va desde los exámenes que preceden y prueban la viabilidad del semental para la recolección hasta la descongelación de las muestras. La recogida se realiza mediante un modelo de vagina artificial para caballos y un maniquí para montar al animal. Después de recolectar y analizar las características macroscópicas (color, apariencia, olor y volumen) y microscópicas (motilidad total, vigor y concentración espermática) del semen fresco, las muestras seminales se enfriarán a 5 ºC durante 24 h con el diluyentes comerciales. Pasado este período, las muestras se centrifugarán y el pellet se resuspendirá con diluyente comercial para congelación y se criopreservará a -196 ºC en pajillas. Las pajitas se descongelarán a 37 ºC para su uso en inseminación artificial.O objetivo com este trabalho é trazer de forma técnica o método de resfriação e congelação de espermatozoides da espécie equina após a refrigeração por 24 h com quatro diluidores comerciais. A abordagem técnica vai desde os exames que antecedem e comprovam a viabilidade do garanhão para coleta até o descongelamento de amostras. A coleta é realizada por meio de vagina artificial modelo para equinos e manequim para monta do animal. Após a coleta e análise das características macroscópicas (cor, aspecto, odor e volume) e microscópicas (motilidade total, vigor e concentração espermática) do sêmen fresco, será realizado o resfriamento das amostras seminais, à 5 ºC, por 24 h, com os diluentes comerciais. Após esse período, as amostras serão centrifugadas e o pellet será ressuspendido com diluente comercial para congelamento e criopreservado à -196 ºC em palhetas. As palhetas serão descongeladas à 37 ºC para serem usadas em inseminação artificial.Research, Society and Development2021-01-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/993910.33448/rsd-v10i1.9939Research, Society and Development; Vol. 10 No. 1; e1831019939Research, Society and Development; Vol. 10 Núm. 1; e1831019939Research, Society and Development; v. 10 n. 1; e18310199392525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/9939/10528Copyright (c) 2021 Rogéria Werner de Almeida Coelho; Julio Cesar Oliveira Diashttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCoelho, Rogéria Werner de AlmeidaDias, Julio Cesar Oliveira2021-02-20T21:19:23Zoai:ojs.pkp.sfu.ca:article/9939Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:32:07.034385Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Freezing equine semen after 24 hours of cooling
Congelación de semen equino después de 24 horas de enfriamiento
Congelação de sêmen equino após 24 horas de resfriamento
title Freezing equine semen after 24 hours of cooling
spellingShingle Freezing equine semen after 24 hours of cooling
Coelho, Rogéria Werner de Almeida
Inseminación artificial
Enfriamiento y congelación de semen
Viabilidad del semental.
Artificial insemination
Semen cooling and freezing
Stallion viability.
Inseminação artificial
Resfriamento e congelamento de sêmen
Viabilidade do garanhão.
title_short Freezing equine semen after 24 hours of cooling
title_full Freezing equine semen after 24 hours of cooling
title_fullStr Freezing equine semen after 24 hours of cooling
title_full_unstemmed Freezing equine semen after 24 hours of cooling
title_sort Freezing equine semen after 24 hours of cooling
author Coelho, Rogéria Werner de Almeida
author_facet Coelho, Rogéria Werner de Almeida
Dias, Julio Cesar Oliveira
author_role author
author2 Dias, Julio Cesar Oliveira
author2_role author
dc.contributor.author.fl_str_mv Coelho, Rogéria Werner de Almeida
Dias, Julio Cesar Oliveira
dc.subject.por.fl_str_mv Inseminación artificial
Enfriamiento y congelación de semen
Viabilidad del semental.
Artificial insemination
Semen cooling and freezing
Stallion viability.
Inseminação artificial
Resfriamento e congelamento de sêmen
Viabilidade do garanhão.
topic Inseminación artificial
Enfriamiento y congelación de semen
Viabilidad del semental.
Artificial insemination
Semen cooling and freezing
Stallion viability.
Inseminação artificial
Resfriamento e congelamento de sêmen
Viabilidade do garanhão.
description The objective of this work is to bring about technically the equine sperm cooling and freezing method after 24 h refrigeration with four commercial diluents. The technical approach ranges from the tests that precede and prove the viability of the stallion for collection to the thawing of samples. The collection is performed by means of artificial model vagina for horses and animal dummy. After collecting and analyzing the macroscopic (color, appearance, odor and volume) and microscopic (total motility, vigor and sperm concentration) characteristics of fresh semen, seminal samples will be cooled at 5 ºC for 24 h. commercial diluents. After this period, the samples will be centrifuged and the pellet will be resuspended with commercial freezing diluent and cryopreserved at - 196 ºC in straws. The blades will be thawed at 37 ° C for use in artificial insemination.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/9939
10.33448/rsd-v10i1.9939
url https://rsdjournal.org/index.php/rsd/article/view/9939
identifier_str_mv 10.33448/rsd-v10i1.9939
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/9939/10528
dc.rights.driver.fl_str_mv Copyright (c) 2021 Rogéria Werner de Almeida Coelho; Julio Cesar Oliveira Dias
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Rogéria Werner de Almeida Coelho; Julio Cesar Oliveira Dias
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 1; e1831019939
Research, Society and Development; Vol. 10 Núm. 1; e1831019939
Research, Society and Development; v. 10 n. 1; e1831019939
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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