EFECT OF MINIMUM CONTAMINATION MEDIUM (MMC) AND MODIFIED EGG YOLK LACTOSE (LGM) EXTENDERS ON CANINE SEMEN VIABILITY AFTER COOLING AT 4 ºC FOR 24 HOURS IN EQUITAINER®

• Autor(a) principal: Barcellos, Bianca Perez
• Data de Publicação: 2011
• Outros Autores: Rocha, Marco Antônio, Jamas, Luciano, Brandão, Felipe Zandonadi, Nogueira, Luis Garcia, Pinho, Tania Goes de
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Ciência animal brasileira (Online)
• Texto Completo: https://revistas.ufg.br/vet/article/view/4692
• topic: Animal reproduction; cães; sêmen; conservação; refrigeração

The aim of this study was to compare the viability of canine semen after dilution in Minimum Contamination Medium (MMC) and Modified Lactose-Egg yolk extender (LGM) and incubation at 4 ºC for 12 and 24 hours. Thirteen ejaculates were collected from 5 adult dogs by digital manipulation. Macroscopic and microscopic characteristics were assessed right after collection. Semen was divided to be diluted (1:3) in MMC and LGM and subsequently chilled in Equitainer®. Seminal parameters of motility and spermatic morphology, membrane integrity (hyposmotic test), spermatic viability and spontaneous acrosome reaction (Trypan-blue Giemsa stain) were evaluated in fresh and chilled semen after 12 and 24 hours of incubation at 4 ºC.  No difference between extenders was identified in semen conservation after 12 hours. After 24 hours just spermatic motility was different (p>0.05). But, after both periods of conservation, semen diluted in LGM maintained most of the characteristics verified in the fresh semen. The mean of true and false acrosome reaction did not exceed 2% in both semen extenders, which demonstrates no influence of media component and incubation period on these phenomena at 4 ºC. In conclusion, these results indicate that both extenders can be used in canine semen conservation at 4 ºC in contêiner during 12 hours without significant changes in semen characteristics. KEYWORDS: Dogs, sperm, cooling.