SEROLOGY AND ISOLATION OF SALMONELLA SP. IN PIGS AT THE FINISHING SITE AND AT SLAUGHTER

Detalhes bibliográficos
Autor(a) principal: Müller, Monika
Data de Publicação: 2009
Outros Autores: Schwarz, Patrícia, Kich, Jalusa Deon, Cardoso, Marisa Ribeiro de Itapema
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência animal brasileira (Online)
Texto Completo: https://revistas.ufg.br/vet/article/view/4492
Resumo: Studies assessing the Salmonella transmission chain in pig herds are the first step to start a control program.  The aims of this study were to compare the prevalence of Salmonella positive pigs at the beginning of the finishing phase and at slaughter, and to identify the possible sources of contamination in the farms. In three finishing farms, environmental swabs from the barns and from the feed silos were collected during the sanitary emptiness. Furthermore, samples of feces and blood from the animals on the day of housing; and aliquots from all feed lots were taken. At slaughter, blood, mesenteric lymph nodes (LM) and intestinal content (CI) were sampled. Blood samples were submitted to a S. Typhimurium ELISA-LPS test. All other samples were submitted to a Salmonella isolation protocol. Feed samples were also submitted to Polymerase Chain Reaction (PCR) targeting the invA gene. Feces samples from all pigs were Salmonella negative at the beginning of the finishing phase, in two farms seropositive animals were found. In two farms, residual environmental contamination was detected, and, in the third farm, one of the feed batches was Salmonella positive on the PCR assay. At slaughter, over 90% of the animals were positive on the ELISA-LPS test and, in all cohorts, a variable number (12%-92%) of carriers in LM and CI was detected. From this on, it was concluded that the finishing phase was critical for the amplification of Salmonella infection, and the residual environmental contamination in the farms as well as Salmonella positive feed batches were the probable infection sources.KEY WORDS: Isolation, finishing and slaughter, Salmonella, serology, swine.
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spelling SEROLOGY AND ISOLATION OF SALMONELLA SP. IN PIGS AT THE FINISHING SITE AND AT SLAUGHTERPERFIL SOROLÓGICO E DE ISOLAMENTO DE Salmonella sp. EM SUÍNOS NO INÍCIO DA TERMINAÇÃO E AO ABATEPig sanitySalmonellasuínossorologiaisolamentoterminaçãoabate.Studies assessing the Salmonella transmission chain in pig herds are the first step to start a control program.  The aims of this study were to compare the prevalence of Salmonella positive pigs at the beginning of the finishing phase and at slaughter, and to identify the possible sources of contamination in the farms. In three finishing farms, environmental swabs from the barns and from the feed silos were collected during the sanitary emptiness. Furthermore, samples of feces and blood from the animals on the day of housing; and aliquots from all feed lots were taken. At slaughter, blood, mesenteric lymph nodes (LM) and intestinal content (CI) were sampled. Blood samples were submitted to a S. Typhimurium ELISA-LPS test. All other samples were submitted to a Salmonella isolation protocol. Feed samples were also submitted to Polymerase Chain Reaction (PCR) targeting the invA gene. Feces samples from all pigs were Salmonella negative at the beginning of the finishing phase, in two farms seropositive animals were found. In two farms, residual environmental contamination was detected, and, in the third farm, one of the feed batches was Salmonella positive on the PCR assay. At slaughter, over 90% of the animals were positive on the ELISA-LPS test and, in all cohorts, a variable number (12%-92%) of carriers in LM and CI was detected. From this on, it was concluded that the finishing phase was critical for the amplification of Salmonella infection, and the residual environmental contamination in the farms as well as Salmonella positive feed batches were the probable infection sources.KEY WORDS: Isolation, finishing and slaughter, Salmonella, serology, swine.Estudos que elucidem a cadeia de transmissão de Salmonella enterica nos sistemas de produção de suínos são importantes para que seja possível implementar programas de controle da infecção. O objetivo deste estudo foi comparar o índice de animais positivos para Salmonella sp. no início da fase de terminação e ao abate e identificar possíveis fontes de contaminação no período. Em três granjas terminadoras, coletaram-se: suabes de superfície nas baias e nos silos durante o vazio sanitário; amostras de fezes e sangue dos animais no dia do alojamento; alíquotas de todos os lotes de ração e amostras de sangue, linfonodos mesentéricos (LM) e conteúdo intestinal (CI) ao abate. As amostras de sangue foram submetidas a teste de ELISA-LPS para Salmonella Typhimurium. Nas demais amostras, pesquisou-se a presença de Salmonella sp. As amostras de ração foram adicionalmente submetidas à técnica da Reação em Cadeia da Polimerase (PCR) amplificando o gene invA. Todos os animais foram negativos para presença de Salmonella sp. nas fezes no início da terminação; entretanto, em duas granjas havia animais soropositivos (12% e 28%, respectivamente). Em duas granjas havia contaminação residual no ambiente e na terceira granja, em um dos lotes de ração, detectou-se a presença de Salmonella sp. pela PCR. Ao abate, acima de 90% dos animais foram positivos no teste de ELISA-LPS, sendo que em todos os lotes encontrou-se um número variável (12-92%) de portadores em LM e CI. A partir disso, concluiu-se que a terminação foi a fase crítica para a amplificação da infecção por Salmonella sp., sendo a presença residual do microrganismo na granja e o fornecimento de ração contaminada fontes prováveis de infecção.PALAVRAS-CHAVES: Abate, isolamento, Salmonella, sorologia, suíno, terminação. Universidade Federal de Goiás2009-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearchAvaliado por paresPesquisa científicaapplication/pdfhttps://revistas.ufg.br/vet/article/view/449210.5216/cab.v10i3.4492Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 10 No. 3 (2009); 931-937Ciência Animal Brasileira / Brazilian Animal Science; v. 10 n. 3 (2009); 931-9371809-68911518-2797reponame:Ciência animal brasileira (Online)instname:Universidade Federal de Goiás (UFG)instacron:UFGporhttps://revistas.ufg.br/vet/article/view/4492/5415Müller, MonikaSchwarz, PatríciaKich, Jalusa DeonCardoso, Marisa Ribeiro de Itapemainfo:eu-repo/semantics/openAccess2009-09-30T20:55:22Zoai:ojs.revistas.ufg.br:article/4492Revistahttps://revistas.ufg.br/vetPUBhttps://revistas.ufg.br/vet/oai||revistacab@gmail.com1809-68911518-2797opendoar:2024-05-21T19:55:15.689312Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)true
dc.title.none.fl_str_mv SEROLOGY AND ISOLATION OF SALMONELLA SP. IN PIGS AT THE FINISHING SITE AND AT SLAUGHTER
PERFIL SOROLÓGICO E DE ISOLAMENTO DE Salmonella sp. EM SUÍNOS NO INÍCIO DA TERMINAÇÃO E AO ABATE
title SEROLOGY AND ISOLATION OF SALMONELLA SP. IN PIGS AT THE FINISHING SITE AND AT SLAUGHTER
spellingShingle SEROLOGY AND ISOLATION OF SALMONELLA SP. IN PIGS AT THE FINISHING SITE AND AT SLAUGHTER
Müller, Monika
Pig sanity
Salmonella
suínos
sorologia
isolamento
terminação
abate.
title_short SEROLOGY AND ISOLATION OF SALMONELLA SP. IN PIGS AT THE FINISHING SITE AND AT SLAUGHTER
title_full SEROLOGY AND ISOLATION OF SALMONELLA SP. IN PIGS AT THE FINISHING SITE AND AT SLAUGHTER
title_fullStr SEROLOGY AND ISOLATION OF SALMONELLA SP. IN PIGS AT THE FINISHING SITE AND AT SLAUGHTER
title_full_unstemmed SEROLOGY AND ISOLATION OF SALMONELLA SP. IN PIGS AT THE FINISHING SITE AND AT SLAUGHTER
title_sort SEROLOGY AND ISOLATION OF SALMONELLA SP. IN PIGS AT THE FINISHING SITE AND AT SLAUGHTER
author Müller, Monika
author_facet Müller, Monika
Schwarz, Patrícia
Kich, Jalusa Deon
Cardoso, Marisa Ribeiro de Itapema
author_role author
author2 Schwarz, Patrícia
Kich, Jalusa Deon
Cardoso, Marisa Ribeiro de Itapema
author2_role author
author
author
dc.contributor.author.fl_str_mv Müller, Monika
Schwarz, Patrícia
Kich, Jalusa Deon
Cardoso, Marisa Ribeiro de Itapema
dc.subject.por.fl_str_mv Pig sanity
Salmonella
suínos
sorologia
isolamento
terminação
abate.
topic Pig sanity
Salmonella
suínos
sorologia
isolamento
terminação
abate.
description Studies assessing the Salmonella transmission chain in pig herds are the first step to start a control program.  The aims of this study were to compare the prevalence of Salmonella positive pigs at the beginning of the finishing phase and at slaughter, and to identify the possible sources of contamination in the farms. In three finishing farms, environmental swabs from the barns and from the feed silos were collected during the sanitary emptiness. Furthermore, samples of feces and blood from the animals on the day of housing; and aliquots from all feed lots were taken. At slaughter, blood, mesenteric lymph nodes (LM) and intestinal content (CI) were sampled. Blood samples were submitted to a S. Typhimurium ELISA-LPS test. All other samples were submitted to a Salmonella isolation protocol. Feed samples were also submitted to Polymerase Chain Reaction (PCR) targeting the invA gene. Feces samples from all pigs were Salmonella negative at the beginning of the finishing phase, in two farms seropositive animals were found. In two farms, residual environmental contamination was detected, and, in the third farm, one of the feed batches was Salmonella positive on the PCR assay. At slaughter, over 90% of the animals were positive on the ELISA-LPS test and, in all cohorts, a variable number (12%-92%) of carriers in LM and CI was detected. From this on, it was concluded that the finishing phase was critical for the amplification of Salmonella infection, and the residual environmental contamination in the farms as well as Salmonella positive feed batches were the probable infection sources.KEY WORDS: Isolation, finishing and slaughter, Salmonella, serology, swine.
publishDate 2009
dc.date.none.fl_str_mv 2009-09-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Research
Avaliado por pares
Pesquisa científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistas.ufg.br/vet/article/view/4492
10.5216/cab.v10i3.4492
url https://revistas.ufg.br/vet/article/view/4492
identifier_str_mv 10.5216/cab.v10i3.4492
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistas.ufg.br/vet/article/view/4492/5415
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Goiás
publisher.none.fl_str_mv Universidade Federal de Goiás
dc.source.none.fl_str_mv Brazilian Animal Science/ Ciência Animal Brasileira; Vol. 10 No. 3 (2009); 931-937
Ciência Animal Brasileira / Brazilian Animal Science; v. 10 n. 3 (2009); 931-937
1809-6891
1518-2797
reponame:Ciência animal brasileira (Online)
instname:Universidade Federal de Goiás (UFG)
instacron:UFG
instname_str Universidade Federal de Goiás (UFG)
instacron_str UFG
institution UFG
reponame_str Ciência animal brasileira (Online)
collection Ciência animal brasileira (Online)
repository.name.fl_str_mv Ciência animal brasileira (Online) - Universidade Federal de Goiás (UFG)
repository.mail.fl_str_mv ||revistacab@gmail.com
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