Vegetative vigor conilon coffee and its potencial for in vitro callus induction
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Coffee Science (Online) |
| Texto Completo: | https://coffeescience.ufla.br/index.php/Coffeescience/article/view/487 |
Resumo: | Considering the importance of the culture of Coffea canephora in the Brazilian Amazon and the need for genetic improvement of this species for cultivation in the state of Rondonia, he objective of this study was to evaluate the correlation between the initial vigor of clonal plants of C. canephora variety Conilon, under field conditions, and its potential for in vitro propagation. Promising clones belonging to the Genetic Improvement Program of Coffea sp were used Embrapa (Brazilian Agricultural Research Corporation) Rondonia. The plant height, number and length of orthotropic shoots in coffee at 10 months after planting were evaluated. In the laboratory, leaves of plants of each clone were segmented into fragments of 1 cm ² which were inoculated on MS medium containing half the concentration of salts, AIB (10 μM), 2,4-D (20 μM) and 2iP (10 μM), sugar (20 g cm-3) and agar (6 g cm-3). Cultures were maintained in a growth chamber under 16 h photoperiod at 2000 lux and 24 ± 2 º C. The callus induction and the percentage of leaf area covered with callus cells were observed within 90 days. Clone 9 showed greater vegetative growth in the field. Only clone 12 showed no callus induced, all other clones were highly responsive to callus induction, and clone 5 showed a higher percentage of leaf area covered with callus cells. There was no phenotypic correlation between early vigor in the field and calogênico potential in vitro, for clones of coffee used. |
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Vegetative vigor conilon coffee and its potencial for in vitro callus inductionVigor vegetativo de café conilon e seu potencial para indução de calos in vitroCoffea canephoravegetative growthplant tissue culturecallus inductionCoffea canephoracrescimento vegetativocultura de tecidos vegetaiscalogêneseConsidering the importance of the culture of Coffea canephora in the Brazilian Amazon and the need for genetic improvement of this species for cultivation in the state of Rondonia, he objective of this study was to evaluate the correlation between the initial vigor of clonal plants of C. canephora variety Conilon, under field conditions, and its potential for in vitro propagation. Promising clones belonging to the Genetic Improvement Program of Coffea sp were used Embrapa (Brazilian Agricultural Research Corporation) Rondonia. The plant height, number and length of orthotropic shoots in coffee at 10 months after planting were evaluated. In the laboratory, leaves of plants of each clone were segmented into fragments of 1 cm ² which were inoculated on MS medium containing half the concentration of salts, AIB (10 μM), 2,4-D (20 μM) and 2iP (10 μM), sugar (20 g cm-3) and agar (6 g cm-3). Cultures were maintained in a growth chamber under 16 h photoperiod at 2000 lux and 24 ± 2 º C. The callus induction and the percentage of leaf area covered with callus cells were observed within 90 days. Clone 9 showed greater vegetative growth in the field. Only clone 12 showed no callus induced, all other clones were highly responsive to callus induction, and clone 5 showed a higher percentage of leaf area covered with callus cells. There was no phenotypic correlation between early vigor in the field and calogênico potential in vitro, for clones of coffee used.Considerando a relevância da cultura de Coffea canephora para a Amazônia brasileira e a necessidade de melhoramento genético dessa espécie para cultivo no estado de Rondônia, objetivou-se, neste trabalho, avaliar a correlação existente entre o vigor vegetativo inicial de plantas clonais de C. canephora var. Conilon, em condições de campo, e seu potencial para propagação in vitro. Foram utilizados clones promissores pertencentes ao Programa de Melhoramento Genético de Coffea sp. da Embrapa Rondônia. Foram avaliadas as características altura da planta, número e comprimento de brotos ortotrópicos, em cafeeiros com 10 meses pós-plantio. Em laboratório, folhas de plantas de cada clone foram segmentadas em fragmentos de 1 cm² os quais foram inoculados em meio MS, contendo metade da concentração dos sais, AIB (10 μM), 2,4-D (20 μM) e 2iP (10 μM), açúcar (20 g cm-3) e ágar (6 g cm-3). As culturas foram mantidas em sala de crescimento, sob fotoperíodo de 16 horas a 2000 lux e 24±2ºC. A indução de calos e a porcentagem de área foliar coberta com células de calo foram observadas nos 90 dias subsequentes. O clone 9 demonstrou maior crescimento vegetativo em campo. Apenas o clone 12 não apresentou calos induzidos, todos os outros clones foram altamente responsivos à indução de calos, e o clone 5 apresentou maior porcentagem de área foliar coberta com células de calo. Não houve correlação fenotípica entre vigor vegetativo inicial em campo e potencial calogênico in vitro, para os clones de cafeeiro utilizados.Editora UFLA2014-03-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/ziphttps://coffeescience.ufla.br/index.php/Coffeescience/article/view/487Coffee Science - ISSN 1984-3909; Vol. 8 No. 4 (2013); 432-438Coffee Science; Vol. 8 Núm. 4 (2013); 432-438Coffee Science; v. 8 n. 4 (2013); 432-4381984-3909reponame:Coffee Science (Online)instname:Universidade Federal de Lavras (UFLA)instacron:UFLAenghttps://coffeescience.ufla.br/index.php/Coffeescience/article/view/487/pdf_61https://coffeescience.ufla.br/index.php/Coffeescience/article/view/487/1129Copyright (c) 2014 Coffee Science - ISSN 1984-3909https://creativecommons.org/info:eu-repo/semantics/openAccessSantos, Maurício Reginaldo Alves dosSantos, Maurício Reginaldo Alves dosFerreira, Maria das Graças RodriguesOliveira, Carla Liegi Lonardoni Gomes deRamalho, André RostandEspíndula, Marcelo Curitiba2014-03-31T13:20:27Zoai:coffeescience.ufla.br:article/487Revistahttps://coffeescience.ufla.br/index.php/CoffeesciencePUBhttps://coffeescience.ufla.br/index.php/Coffeescience/oaicoffeescience@dag.ufla.br||coffeescience@dag.ufla.br|| alvaro-cozadi@hotmail.com1984-39091809-6875opendoar:2024-05-21T19:53:42.714228Coffee Science (Online) - Universidade Federal de Lavras (UFLA)true |
| dc.title.none.fl_str_mv |
Vegetative vigor conilon coffee and its potencial for in vitro callus induction Vigor vegetativo de café conilon e seu potencial para indução de calos in vitro |
| title |
Vegetative vigor conilon coffee and its potencial for in vitro callus induction |
| spellingShingle |
Vegetative vigor conilon coffee and its potencial for in vitro callus induction Santos, Maurício Reginaldo Alves dos Coffea canephora vegetative growth plant tissue culture callus induction Coffea canephora crescimento vegetativo cultura de tecidos vegetais calogênese |
| title_short |
Vegetative vigor conilon coffee and its potencial for in vitro callus induction |
| title_full |
Vegetative vigor conilon coffee and its potencial for in vitro callus induction |
| title_fullStr |
Vegetative vigor conilon coffee and its potencial for in vitro callus induction |
| title_full_unstemmed |
Vegetative vigor conilon coffee and its potencial for in vitro callus induction |
| title_sort |
Vegetative vigor conilon coffee and its potencial for in vitro callus induction |
| author |
Santos, Maurício Reginaldo Alves dos |
| author_facet |
Santos, Maurício Reginaldo Alves dos Ferreira, Maria das Graças Rodrigues Oliveira, Carla Liegi Lonardoni Gomes de Ramalho, André Rostand Espíndula, Marcelo Curitiba |
| author_role |
author |
| author2 |
Ferreira, Maria das Graças Rodrigues Oliveira, Carla Liegi Lonardoni Gomes de Ramalho, André Rostand Espíndula, Marcelo Curitiba |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Maurício Reginaldo Alves dos Santos, Maurício Reginaldo Alves dos Ferreira, Maria das Graças Rodrigues Oliveira, Carla Liegi Lonardoni Gomes de Ramalho, André Rostand Espíndula, Marcelo Curitiba |
| dc.subject.por.fl_str_mv |
Coffea canephora vegetative growth plant tissue culture callus induction Coffea canephora crescimento vegetativo cultura de tecidos vegetais calogênese |
| topic |
Coffea canephora vegetative growth plant tissue culture callus induction Coffea canephora crescimento vegetativo cultura de tecidos vegetais calogênese |
| description |
Considering the importance of the culture of Coffea canephora in the Brazilian Amazon and the need for genetic improvement of this species for cultivation in the state of Rondonia, he objective of this study was to evaluate the correlation between the initial vigor of clonal plants of C. canephora variety Conilon, under field conditions, and its potential for in vitro propagation. Promising clones belonging to the Genetic Improvement Program of Coffea sp were used Embrapa (Brazilian Agricultural Research Corporation) Rondonia. The plant height, number and length of orthotropic shoots in coffee at 10 months after planting were evaluated. In the laboratory, leaves of plants of each clone were segmented into fragments of 1 cm ² which were inoculated on MS medium containing half the concentration of salts, AIB (10 μM), 2,4-D (20 μM) and 2iP (10 μM), sugar (20 g cm-3) and agar (6 g cm-3). Cultures were maintained in a growth chamber under 16 h photoperiod at 2000 lux and 24 ± 2 º C. The callus induction and the percentage of leaf area covered with callus cells were observed within 90 days. Clone 9 showed greater vegetative growth in the field. Only clone 12 showed no callus induced, all other clones were highly responsive to callus induction, and clone 5 showed a higher percentage of leaf area covered with callus cells. There was no phenotypic correlation between early vigor in the field and calogênico potential in vitro, for clones of coffee used. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014-03-29 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://coffeescience.ufla.br/index.php/Coffeescience/article/view/487 |
| url |
https://coffeescience.ufla.br/index.php/Coffeescience/article/view/487 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
https://coffeescience.ufla.br/index.php/Coffeescience/article/view/487/pdf_61 https://coffeescience.ufla.br/index.php/Coffeescience/article/view/487/1129 |
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Copyright (c) 2014 Coffee Science - ISSN 1984-3909 https://creativecommons.org/ info:eu-repo/semantics/openAccess |
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Copyright (c) 2014 Coffee Science - ISSN 1984-3909 https://creativecommons.org/ |
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openAccess |
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application/pdf application/zip |
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Editora UFLA |
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Editora UFLA |
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Coffee Science - ISSN 1984-3909; Vol. 8 No. 4 (2013); 432-438 Coffee Science; Vol. 8 Núm. 4 (2013); 432-438 Coffee Science; v. 8 n. 4 (2013); 432-438 1984-3909 reponame:Coffee Science (Online) instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
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Universidade Federal de Lavras (UFLA) |
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UFLA |
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Coffee Science (Online) |
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Coffee Science (Online) |
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Coffee Science (Online) - Universidade Federal de Lavras (UFLA) |
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coffeescience@dag.ufla.br||coffeescience@dag.ufla.br|| alvaro-cozadi@hotmail.com |
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1799874919554613248 |