An alternative method for Staphylococcus aureus DNA isolation
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004 |
Resumo: | This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl. |
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Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
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An alternative method for Staphylococcus aureus DNA isolationStaphylococcus aureusgenomic DNADNA isolationThis study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl.Universidade Federal de Minas Gerais, Escola de Veterinária2008-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.60 n.2 2008reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352008000200004info:eu-repo/semantics/openAccessChapaval,L.Moon,D.H.Gomes,J.E.Duarte,F.R.Tsai,S.M.eng2008-06-06T00:00:00Zoai:scielo:S0102-09352008000200004Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2008-06-06T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
An alternative method for Staphylococcus aureus DNA isolation |
title |
An alternative method for Staphylococcus aureus DNA isolation |
spellingShingle |
An alternative method for Staphylococcus aureus DNA isolation Chapaval,L. Staphylococcus aureus genomic DNA DNA isolation |
title_short |
An alternative method for Staphylococcus aureus DNA isolation |
title_full |
An alternative method for Staphylococcus aureus DNA isolation |
title_fullStr |
An alternative method for Staphylococcus aureus DNA isolation |
title_full_unstemmed |
An alternative method for Staphylococcus aureus DNA isolation |
title_sort |
An alternative method for Staphylococcus aureus DNA isolation |
author |
Chapaval,L. |
author_facet |
Chapaval,L. Moon,D.H. Gomes,J.E. Duarte,F.R. Tsai,S.M. |
author_role |
author |
author2 |
Moon,D.H. Gomes,J.E. Duarte,F.R. Tsai,S.M. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Chapaval,L. Moon,D.H. Gomes,J.E. Duarte,F.R. Tsai,S.M. |
dc.subject.por.fl_str_mv |
Staphylococcus aureus genomic DNA DNA isolation |
topic |
Staphylococcus aureus genomic DNA DNA isolation |
description |
This study describes a rapid procedure for the isolation of genomic DNA from Staphylococcus aureus that yielded a good amount of high quality DNA for the amplification of staphylococcal enterotoxins genes (A, B, C, D, and E) and the TSST-1 gene as well as enzymatic restriction (HaeIII) from environmental isolates. With this method, it was possible to detect these genes in a sample containing as little as 10(5) cells with positive PCR reactions obtained from approximately 10pg of DNA in a final reaction volume of 25µl. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000200004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-09352008000200004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.60 n.2 2008 reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online) instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
collection |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository.name.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br |
_version_ |
1750220881373691904 |