Development of DNA probe for detection of Aujeszky's disease virus
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001 |
Resumo: | A DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice. |
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Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
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Development of DNA probe for detection of Aujeszky's disease virusAujeszky's disease virusdot-blot hybridizationradioactive probenon radioactive probeA DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice.Universidade Federal de Minas Gerais, Escola de Veterinária1999-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.51 n.4 1999reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09351999000400001info:eu-repo/semantics/openAccessCândido,A.L.Barros,P.P.Resende,M.Flaschart,R.B.eng2001-04-17T00:00:00Zoai:scielo:S0102-09351999000400001Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2001-04-17T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Development of DNA probe for detection of Aujeszky's disease virus |
title |
Development of DNA probe for detection of Aujeszky's disease virus |
spellingShingle |
Development of DNA probe for detection of Aujeszky's disease virus Cândido,A.L. Aujeszky's disease virus dot-blot hybridization radioactive probe non radioactive probe |
title_short |
Development of DNA probe for detection of Aujeszky's disease virus |
title_full |
Development of DNA probe for detection of Aujeszky's disease virus |
title_fullStr |
Development of DNA probe for detection of Aujeszky's disease virus |
title_full_unstemmed |
Development of DNA probe for detection of Aujeszky's disease virus |
title_sort |
Development of DNA probe for detection of Aujeszky's disease virus |
author |
Cândido,A.L. |
author_facet |
Cândido,A.L. Barros,P.P. Resende,M. Flaschart,R.B. |
author_role |
author |
author2 |
Barros,P.P. Resende,M. Flaschart,R.B. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Cândido,A.L. Barros,P.P. Resende,M. Flaschart,R.B. |
dc.subject.por.fl_str_mv |
Aujeszky's disease virus dot-blot hybridization radioactive probe non radioactive probe |
topic |
Aujeszky's disease virus dot-blot hybridization radioactive probe non radioactive probe |
description |
A DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-09351999000400001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.51 n.4 1999 reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online) instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
collection |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository.name.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br |
_version_ |
1750220876161220608 |