Adjustments in the tetrazolium test methodology for assessing the physiological quality of chickpea seeds

• Autor(a) principal: Humberto Alencar Paraíso
• Data de Publicação: 2019
• Outros Autores: Delacyr da Silva Brandão Junior, Ramon Ivo Soares Avelar, Candido Alves da Costa, Luan Souza de Paula Gomes, Warley Marcos Nascimento
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFMG
• Texto Completo: http://dx.doi.org/10.1590/2317-1545v41n1187777; http://hdl.handle.net/1843/39971; https://orcid.org/ 0000-0002-1859-1422
• topic: Cicer arietinum L; seed analysis; viability; Vigor; Grão-de-bico; Sementes - Fisiologia; Sementes - Viabilidade

Quick tests are essential tools to evaluate seed quality. The objective of this study was to adapt the methodology of tetrazolium test, by identifying the most suitable pre-conditioning parameters of imbibition temperature and duration, and tetrazolium solution concentrations, in order to assess the physiological quality of chickpea seeds. Also, this work proposed the separation of lots in classes, according to the viability and vigor of the seeds. Three lots of chickpeas (lot 1 - BRS Aleppo C1, lot 2 - BRS Aleppo basic, and lot 3 - Cicero) were evaluated. They were analyzed according to three combinations of imbibition temperature and duration (41 °C for 4 hours, 41 ºC for 6 hours, and 30 ºC for 18 hours) and two tetrazolium concentrations (0.1% and 0.5%). The imbibition at 41 °C for 4 or 6 hours, and at 30 ºC for 18 hours allowed clear visualization of injuries in the seeds after immersion in 0.1% tetrazolium solution. The intense coloration formed by the 0.5% solution of this salt prevented the differentiation of the types of damage. In addition, it was possible to separate the seeds into four classes: class I (viable and vigorous, without damages), class II (viable and vigorous, with superficial injuries), class III (viable and non vigorous), and class IV (non-viable).