Steric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions

Detalhes bibliográficos
Autor(a) principal: Bruna Mara Aparecida de Carvalho
Data de Publicação: 2016
Outros Autores: Willer Ferreira Silva Júnior, Lorendane Millena Carvalho, Luis Antonio Minim, Gleidson Giordano Pinto de Carvalho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.1590/0104-6632.20160331s20140112
http://hdl.handle.net/1843/42620
https://orcid.org/0000-0001-8980-8599
Resumo: Parameters of equilibrium adsorption obtained from experiments using immobilized metal affinity chromatography (IMAC) were used to evaluate the applicability of the steric mass-action (SMA) model to describe the adsorption of lactoferrin to cryogel resin under different conditions. The adsorption of lactoferrin on continuous supermacroporous cryogel with immobilized Cu2+ ions was evaluated in batch adsorption experiments at different pH (6-8) and temperature (293-313 K) values. Estimated values of the equilibrium constant (K) and characteristic number of binding sites (n) showed that these parameters decreased with increasing ionic strength, pH and temperature, while the nonlinear parameter, the steric factor (σ), increased with increasing ionic strength and temperature. Expressions correlating these parameters with pH, ionic strength and temperature were then derived.
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spelling 2022-06-23T15:24:14Z2022-06-23T15:24:14Z2016331215223https://doi.org/10.1590/0104-6632.20160331s201401120104-6632http://hdl.handle.net/1843/42620https://orcid.org/0000-0001-8980-8599Parameters of equilibrium adsorption obtained from experiments using immobilized metal affinity chromatography (IMAC) were used to evaluate the applicability of the steric mass-action (SMA) model to describe the adsorption of lactoferrin to cryogel resin under different conditions. The adsorption of lactoferrin on continuous supermacroporous cryogel with immobilized Cu2+ ions was evaluated in batch adsorption experiments at different pH (6-8) and temperature (293-313 K) values. Estimated values of the equilibrium constant (K) and characteristic number of binding sites (n) showed that these parameters decreased with increasing ionic strength, pH and temperature, while the nonlinear parameter, the steric factor (σ), increased with increasing ionic strength and temperature. Expressions correlating these parameters with pH, ionic strength and temperature were then derived.engUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASBrazilian Journal of Chemical EngineeringCromatografia de afinidadeProteínasGlicoproteinasSteric mass action model for lactoferrin adsorption in cryogel with immobilized copper ionsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://www.scielo.br/j/bjce/a/VvnCb5bpsSsgPSvrxmnpYgh/?lang=enBruna Mara Aparecida de CarvalhoWiller Ferreira Silva JúniorLorendane Millena CarvalhoLuis Antonio MinimGleidson Giordano Pinto de Carvalhoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/42620/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALSteric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions.pdfSteric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions.pdfapplication/pdf543750https://repositorio.ufmg.br/bitstream/1843/42620/2/Steric%20mass%20action%20model%20for%20lactoferrin%20adsorption%20in%20cryogel%20with%20immobilized%20copper%20ions.pdff8ed91f5fcd2950f126a00bc91dce51eMD521843/426202022-06-23 12:24:15.062oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2022-06-23T15:24:15Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv Steric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions
title Steric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions
spellingShingle Steric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions
Bruna Mara Aparecida de Carvalho
Cromatografia de afinidade
Proteínas
Glicoproteinas
title_short Steric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions
title_full Steric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions
title_fullStr Steric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions
title_full_unstemmed Steric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions
title_sort Steric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions
author Bruna Mara Aparecida de Carvalho
author_facet Bruna Mara Aparecida de Carvalho
Willer Ferreira Silva Júnior
Lorendane Millena Carvalho
Luis Antonio Minim
Gleidson Giordano Pinto de Carvalho
author_role author
author2 Willer Ferreira Silva Júnior
Lorendane Millena Carvalho
Luis Antonio Minim
Gleidson Giordano Pinto de Carvalho
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Bruna Mara Aparecida de Carvalho
Willer Ferreira Silva Júnior
Lorendane Millena Carvalho
Luis Antonio Minim
Gleidson Giordano Pinto de Carvalho
dc.subject.other.pt_BR.fl_str_mv Cromatografia de afinidade
Proteínas
Glicoproteinas
topic Cromatografia de afinidade
Proteínas
Glicoproteinas
description Parameters of equilibrium adsorption obtained from experiments using immobilized metal affinity chromatography (IMAC) were used to evaluate the applicability of the steric mass-action (SMA) model to describe the adsorption of lactoferrin to cryogel resin under different conditions. The adsorption of lactoferrin on continuous supermacroporous cryogel with immobilized Cu2+ ions was evaluated in batch adsorption experiments at different pH (6-8) and temperature (293-313 K) values. Estimated values of the equilibrium constant (K) and characteristic number of binding sites (n) showed that these parameters decreased with increasing ionic strength, pH and temperature, while the nonlinear parameter, the steric factor (σ), increased with increasing ionic strength and temperature. Expressions correlating these parameters with pH, ionic strength and temperature were then derived.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2022-06-23T15:24:14Z
dc.date.available.fl_str_mv 2022-06-23T15:24:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/42620
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.1590/0104-6632.20160331s20140112
dc.identifier.issn.pt_BR.fl_str_mv 0104-6632
dc.identifier.orcid.pt_BR.fl_str_mv https://orcid.org/0000-0001-8980-8599
url https://doi.org/10.1590/0104-6632.20160331s20140112
http://hdl.handle.net/1843/42620
https://orcid.org/0000-0001-8980-8599
identifier_str_mv 0104-6632
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Brazilian Journal of Chemical Engineering
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
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institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
bitstream.url.fl_str_mv https://repositorio.ufmg.br/bitstream/1843/42620/1/License.txt
https://repositorio.ufmg.br/bitstream/1843/42620/2/Steric%20mass%20action%20model%20for%20lactoferrin%20adsorption%20in%20cryogel%20with%20immobilized%20copper%20ions.pdf
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