Long-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/2102 |
Resumo: | Cigarette smoke (CS)-induced emphysema is caused by a continuous inflammatory response in the lower respiratory tract. The development of the condition is believed to be mediated by oxidant–antioxidant imbalance. This paper describes the effects of long-term CS exposure on alveolar cell recruitment, antioxidant defense systems, activity of extracellular matrix metalloelastases, expression of metalloelastase MMP-12, and high mobility group box-1 protein (HMGB-1). Ten C57Bl/6 mice were exposed to 12 cigarettes-a-day for 60 consecutive days, while 10 control animals were exposed to ambient air. After sacrifice, bronchoalveolar lavage fluid (BALF) was removed, and lung tissue underwent biochemical and histological analyses. In CS-exposed animals influx of alveolar macrophages and neutrophils into BALF, lung static elastance, and expression of MMP-12 and HMGB-1 were significantly increased while the activity of antioxidant enzyme was significantly reduced in comparison with control group. Thus, we demonstrated for the first time that long-term CS exposure decreased antioxidant defenses concomitantly with impaired lung function, which was associated with HMGB-1 expression. |
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Bezerra, Frank SilvaValença, Samuel dos SantosPires, Karla Maria PereiraLanzetti, ManuellaPimenta, Wagner AlvesSchmidt, Aline CunhaPorto, Luís Cristovão de Moraes SobrinoZin, Walter Araújo2013-01-30T18:54:15Z2013-01-30T18:54:15Z2011BEZERRA, F. S. et al. Long-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice. Respiratory Physiology & Neurobiology, v. 177, n. 2, p. 120-126, jul. 2011. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1569904811001054>. Acesso em: 29 jan. 2013.15699048http://www.repositorio.ufop.br/handle/123456789/2102Cigarette smoke (CS)-induced emphysema is caused by a continuous inflammatory response in the lower respiratory tract. The development of the condition is believed to be mediated by oxidant–antioxidant imbalance. This paper describes the effects of long-term CS exposure on alveolar cell recruitment, antioxidant defense systems, activity of extracellular matrix metalloelastases, expression of metalloelastase MMP-12, and high mobility group box-1 protein (HMGB-1). Ten C57Bl/6 mice were exposed to 12 cigarettes-a-day for 60 consecutive days, while 10 control animals were exposed to ambient air. After sacrifice, bronchoalveolar lavage fluid (BALF) was removed, and lung tissue underwent biochemical and histological analyses. In CS-exposed animals influx of alveolar macrophages and neutrophils into BALF, lung static elastance, and expression of MMP-12 and HMGB-1 were significantly increased while the activity of antioxidant enzyme was significantly reduced in comparison with control group. Thus, we demonstrated for the first time that long-term CS exposure decreased antioxidant defenses concomitantly with impaired lung function, which was associated with HMGB-1 expression.Cigarette smokeLung inflammationOxidative stressMiceLong-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleO periódico Respiratory Physiology & Neurobiology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3314940983091.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOPLICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://www.repositorio.ufop.br/bitstream/123456789/2102/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALARTIGO_LongTermExposure.pdfARTIGO_LongTermExposure.pdfapplication/pdf435799http://www.repositorio.ufop.br/bitstream/123456789/2102/1/ARTIGO_LongTermExposure.pdf8a4acd2c752c81963dd27aa6975ce30cMD51123456789/21022019-03-19 10:49:23.981oai:localhost: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Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-03-19T14:49:23Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.pt_BR.fl_str_mv |
Long-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice. |
title |
Long-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice. |
spellingShingle |
Long-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice. Bezerra, Frank Silva Cigarette smoke Lung inflammation Oxidative stress Mice |
title_short |
Long-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice. |
title_full |
Long-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice. |
title_fullStr |
Long-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice. |
title_full_unstemmed |
Long-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice. |
title_sort |
Long-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice. |
author |
Bezerra, Frank Silva |
author_facet |
Bezerra, Frank Silva Valença, Samuel dos Santos Pires, Karla Maria Pereira Lanzetti, Manuella Pimenta, Wagner Alves Schmidt, Aline Cunha Porto, Luís Cristovão de Moraes Sobrino Zin, Walter Araújo |
author_role |
author |
author2 |
Valença, Samuel dos Santos Pires, Karla Maria Pereira Lanzetti, Manuella Pimenta, Wagner Alves Schmidt, Aline Cunha Porto, Luís Cristovão de Moraes Sobrino Zin, Walter Araújo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Bezerra, Frank Silva Valença, Samuel dos Santos Pires, Karla Maria Pereira Lanzetti, Manuella Pimenta, Wagner Alves Schmidt, Aline Cunha Porto, Luís Cristovão de Moraes Sobrino Zin, Walter Araújo |
dc.subject.por.fl_str_mv |
Cigarette smoke Lung inflammation Oxidative stress Mice |
topic |
Cigarette smoke Lung inflammation Oxidative stress Mice |
description |
Cigarette smoke (CS)-induced emphysema is caused by a continuous inflammatory response in the lower respiratory tract. The development of the condition is believed to be mediated by oxidant–antioxidant imbalance. This paper describes the effects of long-term CS exposure on alveolar cell recruitment, antioxidant defense systems, activity of extracellular matrix metalloelastases, expression of metalloelastase MMP-12, and high mobility group box-1 protein (HMGB-1). Ten C57Bl/6 mice were exposed to 12 cigarettes-a-day for 60 consecutive days, while 10 control animals were exposed to ambient air. After sacrifice, bronchoalveolar lavage fluid (BALF) was removed, and lung tissue underwent biochemical and histological analyses. In CS-exposed animals influx of alveolar macrophages and neutrophils into BALF, lung static elastance, and expression of MMP-12 and HMGB-1 were significantly increased while the activity of antioxidant enzyme was significantly reduced in comparison with control group. Thus, we demonstrated for the first time that long-term CS exposure decreased antioxidant defenses concomitantly with impaired lung function, which was associated with HMGB-1 expression. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2013-01-30T18:54:15Z |
dc.date.available.fl_str_mv |
2013-01-30T18:54:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BEZERRA, F. S. et al. Long-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice. Respiratory Physiology & Neurobiology, v. 177, n. 2, p. 120-126, jul. 2011. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1569904811001054>. Acesso em: 29 jan. 2013. |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufop.br/handle/123456789/2102 |
dc.identifier.issn.none.fl_str_mv |
15699048 |
identifier_str_mv |
BEZERRA, F. S. et al. Long-term exposure to cigarette smoke impairs lung function and increases HMGB-1 expression in mice. Respiratory Physiology & Neurobiology, v. 177, n. 2, p. 120-126, jul. 2011. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1569904811001054>. Acesso em: 29 jan. 2013. 15699048 |
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http://www.repositorio.ufop.br/handle/123456789/2102 |
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eng |
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