Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis.

Detalhes bibliográficos
Autor(a) principal: Oldoni, Tatiane Luiza Cadorin
Data de Publicação: 2011
Outros Autores: Cabral, Ingridy Simone Ribeiro, d’Arce, Marisa Aparecida Bismara Regitano, Rosalen, Pedro Luiz, Ikegaki, Masaharu, Nascimento, Andréa Mendes do, Alencar, Severino Matias de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFOP
Texto Completo: http://www.repositorio.ufop.br/handle/123456789/1581
Resumo: Activity-directed fractionation and purification processes were employed to identify isoflavonoids with antioxidant and antimicrobial activities from Brazilian red propolis. Crude propolis was extracted with ethanol (80%, v/v) and fractioned by liquid–liquid extraction technique using hexane and chloroform. Since chloroform fraction showed strong antioxidant and antimicrobial activities it was purified and isolated using various chromatographic techniques. Comparing our spectral data (UV, NMR, and mass spectrometry) with values found in the literature, we identified two bioactiveisoflavonoids (vestitol and neovestitol), together with one chalcone (isoliquiritigenin). Vestitol presented higher antioxidant activity against β-carotene consumption than neovestitol. The antimicrobial activity of these three compounds against Staphylococcus aureus, Streptococcus mutans, and Actinomyces naeslundii was evaluated and we concluded that isoliquiritigenin was the most active one with lower MIC, ranging from 15.6 to 62.5 μg/mL. Our results showed that Brazilian red propolis has biologically active isoflavonoids that may be used as a mild antioxidant and antimicrobial for food preservation.
id UFOP_be2bc91619b9c4fe33ae6669c0759f9e
oai_identifier_str oai:repositorio.ufop.br:123456789/1581
network_acronym_str UFOP
network_name_str Repositório Institucional da UFOP
repository_id_str 3233
spelling Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis.Antioxidant activityBrazilian propolisAntimicrobial activityIsoflavonoidsChalconeActivity-directed fractionation and purification processes were employed to identify isoflavonoids with antioxidant and antimicrobial activities from Brazilian red propolis. Crude propolis was extracted with ethanol (80%, v/v) and fractioned by liquid–liquid extraction technique using hexane and chloroform. Since chloroform fraction showed strong antioxidant and antimicrobial activities it was purified and isolated using various chromatographic techniques. Comparing our spectral data (UV, NMR, and mass spectrometry) with values found in the literature, we identified two bioactiveisoflavonoids (vestitol and neovestitol), together with one chalcone (isoliquiritigenin). Vestitol presented higher antioxidant activity against β-carotene consumption than neovestitol. The antimicrobial activity of these three compounds against Staphylococcus aureus, Streptococcus mutans, and Actinomyces naeslundii was evaluated and we concluded that isoliquiritigenin was the most active one with lower MIC, ranging from 15.6 to 62.5 μg/mL. Our results showed that Brazilian red propolis has biologically active isoflavonoids that may be used as a mild antioxidant and antimicrobial for food preservation.2012-10-09T12:23:37Z2012-10-09T12:23:37Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfOLDONI, T. L. C. et al. Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. Separation and Purification Technology, v. 77, n. 2, p. 208-213, fev. 2011. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1383586610005058>. Acesso em: 09 out. 2012.13835866http://www.repositorio.ufop.br/handle/123456789/1581O periódico Separation and Purification Technology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3310910726398.info:eu-repo/semantics/openAccessOldoni, Tatiane Luiza CadorinCabral, Ingridy Simone Ribeirod’Arce, Marisa Aparecida Bismara RegitanoRosalen, Pedro LuizIkegaki, MasaharuNascimento, Andréa Mendes doAlencar, Severino Matias deengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-03-11T17:43:41Zoai:repositorio.ufop.br:123456789/1581Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-03-11T17:43:41Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false
dc.title.none.fl_str_mv Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis.
title Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis.
spellingShingle Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis.
Oldoni, Tatiane Luiza Cadorin
Antioxidant activity
Brazilian propolis
Antimicrobial activity
Isoflavonoids
Chalcone
title_short Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis.
title_full Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis.
title_fullStr Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis.
title_full_unstemmed Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis.
title_sort Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis.
author Oldoni, Tatiane Luiza Cadorin
author_facet Oldoni, Tatiane Luiza Cadorin
Cabral, Ingridy Simone Ribeiro
d’Arce, Marisa Aparecida Bismara Regitano
Rosalen, Pedro Luiz
Ikegaki, Masaharu
Nascimento, Andréa Mendes do
Alencar, Severino Matias de
author_role author
author2 Cabral, Ingridy Simone Ribeiro
d’Arce, Marisa Aparecida Bismara Regitano
Rosalen, Pedro Luiz
Ikegaki, Masaharu
Nascimento, Andréa Mendes do
Alencar, Severino Matias de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oldoni, Tatiane Luiza Cadorin
Cabral, Ingridy Simone Ribeiro
d’Arce, Marisa Aparecida Bismara Regitano
Rosalen, Pedro Luiz
Ikegaki, Masaharu
Nascimento, Andréa Mendes do
Alencar, Severino Matias de
dc.subject.por.fl_str_mv Antioxidant activity
Brazilian propolis
Antimicrobial activity
Isoflavonoids
Chalcone
topic Antioxidant activity
Brazilian propolis
Antimicrobial activity
Isoflavonoids
Chalcone
description Activity-directed fractionation and purification processes were employed to identify isoflavonoids with antioxidant and antimicrobial activities from Brazilian red propolis. Crude propolis was extracted with ethanol (80%, v/v) and fractioned by liquid–liquid extraction technique using hexane and chloroform. Since chloroform fraction showed strong antioxidant and antimicrobial activities it was purified and isolated using various chromatographic techniques. Comparing our spectral data (UV, NMR, and mass spectrometry) with values found in the literature, we identified two bioactiveisoflavonoids (vestitol and neovestitol), together with one chalcone (isoliquiritigenin). Vestitol presented higher antioxidant activity against β-carotene consumption than neovestitol. The antimicrobial activity of these three compounds against Staphylococcus aureus, Streptococcus mutans, and Actinomyces naeslundii was evaluated and we concluded that isoliquiritigenin was the most active one with lower MIC, ranging from 15.6 to 62.5 μg/mL. Our results showed that Brazilian red propolis has biologically active isoflavonoids that may be used as a mild antioxidant and antimicrobial for food preservation.
publishDate 2011
dc.date.none.fl_str_mv 2011
2012-10-09T12:23:37Z
2012-10-09T12:23:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv OLDONI, T. L. C. et al. Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. Separation and Purification Technology, v. 77, n. 2, p. 208-213, fev. 2011. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1383586610005058>. Acesso em: 09 out. 2012.
13835866
http://www.repositorio.ufop.br/handle/123456789/1581
identifier_str_mv OLDONI, T. L. C. et al. Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. Separation and Purification Technology, v. 77, n. 2, p. 208-213, fev. 2011. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1383586610005058>. Acesso em: 09 out. 2012.
13835866
url http://www.repositorio.ufop.br/handle/123456789/1581
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFOP
instname:Universidade Federal de Ouro Preto (UFOP)
instacron:UFOP
instname_str Universidade Federal de Ouro Preto (UFOP)
instacron_str UFOP
institution UFOP
reponame_str Repositório Institucional da UFOP
collection Repositório Institucional da UFOP
repository.name.fl_str_mv Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)
repository.mail.fl_str_mv repositorio@ufop.edu.br
_version_ 1813002861418643456