Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFOP |
Texto Completo: | http://www.repositorio.ufop.br/handle/123456789/1581 |
Resumo: | Activity-directed fractionation and purification processes were employed to identify isoflavonoids with antioxidant and antimicrobial activities from Brazilian red propolis. Crude propolis was extracted with ethanol (80%, v/v) and fractioned by liquid–liquid extraction technique using hexane and chloroform. Since chloroform fraction showed strong antioxidant and antimicrobial activities it was purified and isolated using various chromatographic techniques. Comparing our spectral data (UV, NMR, and mass spectrometry) with values found in the literature, we identified two bioactiveisoflavonoids (vestitol and neovestitol), together with one chalcone (isoliquiritigenin). Vestitol presented higher antioxidant activity against β-carotene consumption than neovestitol. The antimicrobial activity of these three compounds against Staphylococcus aureus, Streptococcus mutans, and Actinomyces naeslundii was evaluated and we concluded that isoliquiritigenin was the most active one with lower MIC, ranging from 15.6 to 62.5 μg/mL. Our results showed that Brazilian red propolis has biologically active isoflavonoids that may be used as a mild antioxidant and antimicrobial for food preservation. |
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Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis.Antioxidant activityBrazilian propolisAntimicrobial activityIsoflavonoidsChalconeActivity-directed fractionation and purification processes were employed to identify isoflavonoids with antioxidant and antimicrobial activities from Brazilian red propolis. Crude propolis was extracted with ethanol (80%, v/v) and fractioned by liquid–liquid extraction technique using hexane and chloroform. Since chloroform fraction showed strong antioxidant and antimicrobial activities it was purified and isolated using various chromatographic techniques. Comparing our spectral data (UV, NMR, and mass spectrometry) with values found in the literature, we identified two bioactiveisoflavonoids (vestitol and neovestitol), together with one chalcone (isoliquiritigenin). Vestitol presented higher antioxidant activity against β-carotene consumption than neovestitol. The antimicrobial activity of these three compounds against Staphylococcus aureus, Streptococcus mutans, and Actinomyces naeslundii was evaluated and we concluded that isoliquiritigenin was the most active one with lower MIC, ranging from 15.6 to 62.5 μg/mL. Our results showed that Brazilian red propolis has biologically active isoflavonoids that may be used as a mild antioxidant and antimicrobial for food preservation.2012-10-09T12:23:37Z2012-10-09T12:23:37Z2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfOLDONI, T. L. C. et al. Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. Separation and Purification Technology, v. 77, n. 2, p. 208-213, fev. 2011. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1383586610005058>. Acesso em: 09 out. 2012.13835866http://www.repositorio.ufop.br/handle/123456789/1581O periódico Separation and Purification Technology concede permissão para depósito deste artigo no Repositório Institucional da UFOP. Número da licença: 3310910726398.info:eu-repo/semantics/openAccessOldoni, Tatiane Luiza CadorinCabral, Ingridy Simone Ribeirod’Arce, Marisa Aparecida Bismara RegitanoRosalen, Pedro LuizIkegaki, MasaharuNascimento, Andréa Mendes doAlencar, Severino Matias deengreponame:Repositório Institucional da UFOPinstname:Universidade Federal de Ouro Preto (UFOP)instacron:UFOP2019-03-11T17:43:41Zoai:repositorio.ufop.br:123456789/1581Repositório InstitucionalPUBhttp://www.repositorio.ufop.br/oai/requestrepositorio@ufop.edu.bropendoar:32332019-03-11T17:43:41Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP)false |
dc.title.none.fl_str_mv |
Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. |
title |
Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. |
spellingShingle |
Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. Oldoni, Tatiane Luiza Cadorin Antioxidant activity Brazilian propolis Antimicrobial activity Isoflavonoids Chalcone |
title_short |
Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. |
title_full |
Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. |
title_fullStr |
Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. |
title_full_unstemmed |
Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. |
title_sort |
Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. |
author |
Oldoni, Tatiane Luiza Cadorin |
author_facet |
Oldoni, Tatiane Luiza Cadorin Cabral, Ingridy Simone Ribeiro d’Arce, Marisa Aparecida Bismara Regitano Rosalen, Pedro Luiz Ikegaki, Masaharu Nascimento, Andréa Mendes do Alencar, Severino Matias de |
author_role |
author |
author2 |
Cabral, Ingridy Simone Ribeiro d’Arce, Marisa Aparecida Bismara Regitano Rosalen, Pedro Luiz Ikegaki, Masaharu Nascimento, Andréa Mendes do Alencar, Severino Matias de |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Oldoni, Tatiane Luiza Cadorin Cabral, Ingridy Simone Ribeiro d’Arce, Marisa Aparecida Bismara Regitano Rosalen, Pedro Luiz Ikegaki, Masaharu Nascimento, Andréa Mendes do Alencar, Severino Matias de |
dc.subject.por.fl_str_mv |
Antioxidant activity Brazilian propolis Antimicrobial activity Isoflavonoids Chalcone |
topic |
Antioxidant activity Brazilian propolis Antimicrobial activity Isoflavonoids Chalcone |
description |
Activity-directed fractionation and purification processes were employed to identify isoflavonoids with antioxidant and antimicrobial activities from Brazilian red propolis. Crude propolis was extracted with ethanol (80%, v/v) and fractioned by liquid–liquid extraction technique using hexane and chloroform. Since chloroform fraction showed strong antioxidant and antimicrobial activities it was purified and isolated using various chromatographic techniques. Comparing our spectral data (UV, NMR, and mass spectrometry) with values found in the literature, we identified two bioactiveisoflavonoids (vestitol and neovestitol), together with one chalcone (isoliquiritigenin). Vestitol presented higher antioxidant activity against β-carotene consumption than neovestitol. The antimicrobial activity of these three compounds against Staphylococcus aureus, Streptococcus mutans, and Actinomyces naeslundii was evaluated and we concluded that isoliquiritigenin was the most active one with lower MIC, ranging from 15.6 to 62.5 μg/mL. Our results showed that Brazilian red propolis has biologically active isoflavonoids that may be used as a mild antioxidant and antimicrobial for food preservation. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2012-10-09T12:23:37Z 2012-10-09T12:23:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
OLDONI, T. L. C. et al. Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. Separation and Purification Technology, v. 77, n. 2, p. 208-213, fev. 2011. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1383586610005058>. Acesso em: 09 out. 2012. 13835866 http://www.repositorio.ufop.br/handle/123456789/1581 |
identifier_str_mv |
OLDONI, T. L. C. et al. Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis. Separation and Purification Technology, v. 77, n. 2, p. 208-213, fev. 2011. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1383586610005058>. Acesso em: 09 out. 2012. 13835866 |
url |
http://www.repositorio.ufop.br/handle/123456789/1581 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFOP instname:Universidade Federal de Ouro Preto (UFOP) instacron:UFOP |
instname_str |
Universidade Federal de Ouro Preto (UFOP) |
instacron_str |
UFOP |
institution |
UFOP |
reponame_str |
Repositório Institucional da UFOP |
collection |
Repositório Institucional da UFOP |
repository.name.fl_str_mv |
Repositório Institucional da UFOP - Universidade Federal de Ouro Preto (UFOP) |
repository.mail.fl_str_mv |
repositorio@ufop.edu.br |
_version_ |
1813002861418643456 |