Biodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial site
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Tese |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFPE |
Texto Completo: | https://repositorio.ufpe.br/handle/123456789/47048 |
Resumo: | When attempting to remediate contaminated sites it is important to have a good understanding of the sites’ physical, chemical, and hydrogeological properties, so that flow of contaminants can be predicted and evaluated. The contribution of biological processes is also of significance and needs to be better understood to improve the remediation strategy and process. Aerobic biotransformations of contaminants are often observed in the shallow layers, while anaerobic biotransformations processes are established in the deeper, more anoxic layers, including groundwater. Aromatic compounds, such as pesticides, dyes, resins, and solvents, are a major group of contaminants originating from a variety of industries. Aiming to combine academic research with remediation projects carried out by the industry partner, this Ph.D. thesis intended to contribute to bioremediation projects considering complex pollutants, such as halogenated aromatic compounds. To evaluate the biodegradability of dichloronitrobenzene (DCNB), dichloroaniline (DCA), monochloroaniline (MCA) and aniline by native microbes from a contaminated site, we first enriched cultures inoculated with soil and groundwater from a contaminated site, under aerobic and anaerobic conditions, amended with the target compounds individually, in the range of 10 and 20 mg/L. In addition to the inoculum and the contaminants, the enrichments contained mineral medium and ethanol and lactate as the electron donors (the anaerobic one). The cultures were kept in Boston glass bottles of 250 mL (150 mL of headspace). By enriching the cultures through multiple re-feedings, we could start to describe the mechanisms of biotransformation and identify organisms potentially involved in the observed reactions. Under aerobic conditions, 2,3-DCA, 3,4-DCA, 2-MCA, 3-MCA, and 4-MCA were potentially mineralized by members of the Pandoraea and Burkholderia-Caballero-Paraburkholderia genera, while chloride and ammonium ions were released. The anaerobic enrichments revealed nitro reduction of the 2,3-, 3,4-, and 2,5-DCNB by fermentative bacteria through a likely co-metabolic process, forming DCA isomers. Desulfitobacterium may be implicated in nitro group reduction in these enrichments, however, further investigation is needed for confirmation. The 2,3- and 3,4- DCA were reductively dehalogenated by organohalide-respiring bacteria, specifically Dehalobacter and Anaeromyxobacter genera. The well-established and active anaerobic community is composed of fermentative bacteria, organohalide-respiring bacteria, sulphate- reducing bacteria, and archaea. In general, from our lab experiments, we can affirm that bioremediation with native microbes from the site is a promising strategy to be applied in the contaminated area. Bioaugmentation seems not to be necessary, but biostimulation through addition of oxygen should be considered since natural attenuation under anaerobic conditions did not result in complete dechlorination and mineralize the contaminants. |
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ARAÚJO, Sofia Pimentelhttp://lattes.cnpq.br/2125054205778617http://lattes.cnpq.br/3117559199438663PESSÔA, Sávia Gavazza dos SantosEDWARDS, Elizabeth Anne2022-10-14T11:44:58Z2022-10-14T11:44:58Z2022-09-12ARAÚJO, Sofia Pimentel. Biodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial site. 2022. Tese (Doutorado em Engenharia Civil) - Universidade Federal de Pernambuco, Recife, 2022.https://repositorio.ufpe.br/handle/123456789/47048When attempting to remediate contaminated sites it is important to have a good understanding of the sites’ physical, chemical, and hydrogeological properties, so that flow of contaminants can be predicted and evaluated. The contribution of biological processes is also of significance and needs to be better understood to improve the remediation strategy and process. Aerobic biotransformations of contaminants are often observed in the shallow layers, while anaerobic biotransformations processes are established in the deeper, more anoxic layers, including groundwater. Aromatic compounds, such as pesticides, dyes, resins, and solvents, are a major group of contaminants originating from a variety of industries. Aiming to combine academic research with remediation projects carried out by the industry partner, this Ph.D. thesis intended to contribute to bioremediation projects considering complex pollutants, such as halogenated aromatic compounds. To evaluate the biodegradability of dichloronitrobenzene (DCNB), dichloroaniline (DCA), monochloroaniline (MCA) and aniline by native microbes from a contaminated site, we first enriched cultures inoculated with soil and groundwater from a contaminated site, under aerobic and anaerobic conditions, amended with the target compounds individually, in the range of 10 and 20 mg/L. In addition to the inoculum and the contaminants, the enrichments contained mineral medium and ethanol and lactate as the electron donors (the anaerobic one). The cultures were kept in Boston glass bottles of 250 mL (150 mL of headspace). By enriching the cultures through multiple re-feedings, we could start to describe the mechanisms of biotransformation and identify organisms potentially involved in the observed reactions. Under aerobic conditions, 2,3-DCA, 3,4-DCA, 2-MCA, 3-MCA, and 4-MCA were potentially mineralized by members of the Pandoraea and Burkholderia-Caballero-Paraburkholderia genera, while chloride and ammonium ions were released. The anaerobic enrichments revealed nitro reduction of the 2,3-, 3,4-, and 2,5-DCNB by fermentative bacteria through a likely co-metabolic process, forming DCA isomers. Desulfitobacterium may be implicated in nitro group reduction in these enrichments, however, further investigation is needed for confirmation. The 2,3- and 3,4- DCA were reductively dehalogenated by organohalide-respiring bacteria, specifically Dehalobacter and Anaeromyxobacter genera. The well-established and active anaerobic community is composed of fermentative bacteria, organohalide-respiring bacteria, sulphate- reducing bacteria, and archaea. In general, from our lab experiments, we can affirm that bioremediation with native microbes from the site is a promising strategy to be applied in the contaminated area. Bioaugmentation seems not to be necessary, but biostimulation through addition of oxygen should be considered since natural attenuation under anaerobic conditions did not result in complete dechlorination and mineralize the contaminants.FACEPECAPESCNPqPara remediação de áreas contaminadas, o entendimento das propriedades físicas, químicas e hidrogeológicas dos locais é essencial para que o fluxo de contaminantes possa ser previsto e avaliado. Além disso, a contribuição dos processos biológicos é também significativamente importante e precisa ser compreendida para impulsionar a estratégia e o processo de remediação. A biotransformação aeróbia de contaminantes é frequentemente observada nas camadas mais superficiais do solo, enquanto os processos de biotransformação anaeróbia são estabelecidos nas camadas mais profundas e anóxicas, incluindo as águas subterrâneas. Compostos aromáticos, como pesticidas, corantes, resinas e solventes, são um grande grupo de contaminantes originários de uma variedade de indústrias. Com o objetivo de alinhar pesquisas acadêmicas com projetos de remediação realizados pela indústria parceira desta pesquisa, este trabalho pretende contribuir para projetos de biorremediação considerando poluentes complexos, como compostos aromáticos halogenados. Para avaliar a biodegradabilidade de dicloronitrobenzeno (DCNB), dicloroanilina (DCA), monocloroanilina (MCA) e anilina por microrganismos nativos de uma área industrial contaminada, culturas inoculadas com solo e água subterrânea do local em estudo foram enriquecidas sob condições aeróbias e anaeróbias, alimentadas com os compostos alvo individualmente, na faixa de 10 e 20 mg/L. Além do inóculo e dos contaminantes, os enriquecimentos continham meio mineral e etanol e lactato como doadores de elétrons (somente os anaeróbios). As culturas foram mantidas em frascos de vidro do tipo Boston de 250 mL (150 mL de headspace). Ao enriquecer as culturas através de múltiplas realimentações, foi possível descrever os possíveis mecanismos de biotransformação e identificar organismos potencialmente envolvidos nas reações observadas. Sob condições aeróbias, houve provável mineralização dos contaminantes 2,3-DCA, 3,4-DCA, 2-MCA, 3-MCA e 4-MCA por membros dos gêneros Pandoraea e Burkholderia-Caballero-Paraburkholderia, enquanto íons cloreto e amônio foram liberados no meio. Os enriquecimentos anaeróbios revelaram redução do grupo nitro dos isômeros de dichloronitrobenzeno (2,3-, 3,4- e 2,5-DCNB) por bactérias fermentadoras através de um provável processo co-metabólico, formando isômeros de DCA. É possível que organismos do gênero Desulfitobacterium estejam envolvidos na redução do grupo nitro nestes enriquecimentos; no entanto, mais investigações são necessárias para confirmação. A remoção do primeiro cloro dos isômeros 2,3- e 3,4-DCA se deu através da respiração de bactérias dos gêneros Dehalobacter e Anaeromyxobacter. A comunidade anaeróbia bem estabelecida e ativa é composta por bactérias fermentativas, bactérias que respiram organohaletos, bactérias redutoras de sulfato e arqueias. Em geral, a partir de nossos experimentos de laboratório, pode-se afirmar que a biorremediação com microrganismos nativos do solo e da água subterrânea é uma estratégia promissora a ser aplicada na área industrial contaminada. Dessa forma, a bioaumentação pode não ser necessária, mas a bioestimulação através da adição de oxigênio deve ser considerada, uma vez que a atenuação natural em condições anaeróbias não houve mineralização dos contaminantes.engUniversidade Federal de PernambucoPrograma de Pos Graduacao em Engenharia CivilUFPEBrasilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/info:eu-repo/semantics/embargoedAccessEngenharia civilDicloronitrobenzenoDicloroanilinaMonocloroanilinaBiodegradação aeróbiaBiotransformação anaeróbiaBiorremediaçãoBiodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial siteinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisdoutoradoreponame:Repositório Institucional da UFPEinstname:Universidade Federal de Pernambuco (UFPE)instacron:UFPELICENSElicense.txtlicense.txttext/plain; charset=utf-82362https://repositorio.ufpe.br/bitstream/123456789/47048/3/license.txt5e89a1613ddc8510c6576f4b23a78973MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.pt_BR.fl_str_mv |
Biodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial site |
title |
Biodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial site |
spellingShingle |
Biodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial site ARAÚJO, Sofia Pimentel Engenharia civil Dicloronitrobenzeno Dicloroanilina Monocloroanilina Biodegradação aeróbia Biotransformação anaeróbia Biorremediação |
title_short |
Biodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial site |
title_full |
Biodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial site |
title_fullStr |
Biodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial site |
title_full_unstemmed |
Biodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial site |
title_sort |
Biodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial site |
author |
ARAÚJO, Sofia Pimentel |
author_facet |
ARAÚJO, Sofia Pimentel |
author_role |
author |
dc.contributor.authorLattes.pt_BR.fl_str_mv |
http://lattes.cnpq.br/2125054205778617 |
dc.contributor.advisorLattes.pt_BR.fl_str_mv |
http://lattes.cnpq.br/3117559199438663 |
dc.contributor.author.fl_str_mv |
ARAÚJO, Sofia Pimentel |
dc.contributor.advisor1.fl_str_mv |
PESSÔA, Sávia Gavazza dos Santos |
dc.contributor.advisor-co1.fl_str_mv |
EDWARDS, Elizabeth Anne |
contributor_str_mv |
PESSÔA, Sávia Gavazza dos Santos EDWARDS, Elizabeth Anne |
dc.subject.por.fl_str_mv |
Engenharia civil Dicloronitrobenzeno Dicloroanilina Monocloroanilina Biodegradação aeróbia Biotransformação anaeróbia Biorremediação |
topic |
Engenharia civil Dicloronitrobenzeno Dicloroanilina Monocloroanilina Biodegradação aeróbia Biotransformação anaeróbia Biorremediação |
description |
When attempting to remediate contaminated sites it is important to have a good understanding of the sites’ physical, chemical, and hydrogeological properties, so that flow of contaminants can be predicted and evaluated. The contribution of biological processes is also of significance and needs to be better understood to improve the remediation strategy and process. Aerobic biotransformations of contaminants are often observed in the shallow layers, while anaerobic biotransformations processes are established in the deeper, more anoxic layers, including groundwater. Aromatic compounds, such as pesticides, dyes, resins, and solvents, are a major group of contaminants originating from a variety of industries. Aiming to combine academic research with remediation projects carried out by the industry partner, this Ph.D. thesis intended to contribute to bioremediation projects considering complex pollutants, such as halogenated aromatic compounds. To evaluate the biodegradability of dichloronitrobenzene (DCNB), dichloroaniline (DCA), monochloroaniline (MCA) and aniline by native microbes from a contaminated site, we first enriched cultures inoculated with soil and groundwater from a contaminated site, under aerobic and anaerobic conditions, amended with the target compounds individually, in the range of 10 and 20 mg/L. In addition to the inoculum and the contaminants, the enrichments contained mineral medium and ethanol and lactate as the electron donors (the anaerobic one). The cultures were kept in Boston glass bottles of 250 mL (150 mL of headspace). By enriching the cultures through multiple re-feedings, we could start to describe the mechanisms of biotransformation and identify organisms potentially involved in the observed reactions. Under aerobic conditions, 2,3-DCA, 3,4-DCA, 2-MCA, 3-MCA, and 4-MCA were potentially mineralized by members of the Pandoraea and Burkholderia-Caballero-Paraburkholderia genera, while chloride and ammonium ions were released. The anaerobic enrichments revealed nitro reduction of the 2,3-, 3,4-, and 2,5-DCNB by fermentative bacteria through a likely co-metabolic process, forming DCA isomers. Desulfitobacterium may be implicated in nitro group reduction in these enrichments, however, further investigation is needed for confirmation. The 2,3- and 3,4- DCA were reductively dehalogenated by organohalide-respiring bacteria, specifically Dehalobacter and Anaeromyxobacter genera. The well-established and active anaerobic community is composed of fermentative bacteria, organohalide-respiring bacteria, sulphate- reducing bacteria, and archaea. In general, from our lab experiments, we can affirm that bioremediation with native microbes from the site is a promising strategy to be applied in the contaminated area. Bioaugmentation seems not to be necessary, but biostimulation through addition of oxygen should be considered since natural attenuation under anaerobic conditions did not result in complete dechlorination and mineralize the contaminants. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-10-14T11:44:58Z |
dc.date.available.fl_str_mv |
2022-10-14T11:44:58Z |
dc.date.issued.fl_str_mv |
2022-09-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARAÚJO, Sofia Pimentel. Biodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial site. 2022. Tese (Doutorado em Engenharia Civil) - Universidade Federal de Pernambuco, Recife, 2022. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufpe.br/handle/123456789/47048 |
identifier_str_mv |
ARAÚJO, Sofia Pimentel. Biodegradation of nitro and chlorinated aromatic compounds for bioremediation of a contaminated industrial site. 2022. Tese (Doutorado em Engenharia Civil) - Universidade Federal de Pernambuco, Recife, 2022. |
url |
https://repositorio.ufpe.br/handle/123456789/47048 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/3.0/br/ info:eu-repo/semantics/embargoedAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/3.0/br/ |
eu_rights_str_mv |
embargoedAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Pernambuco |
dc.publisher.program.fl_str_mv |
Programa de Pos Graduacao em Engenharia Civil |
dc.publisher.initials.fl_str_mv |
UFPE |
dc.publisher.country.fl_str_mv |
Brasil |
publisher.none.fl_str_mv |
Universidade Federal de Pernambuco |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFPE instname:Universidade Federal de Pernambuco (UFPE) instacron:UFPE |
instname_str |
Universidade Federal de Pernambuco (UFPE) |
instacron_str |
UFPE |
institution |
UFPE |
reponame_str |
Repositório Institucional da UFPE |
collection |
Repositório Institucional da UFPE |
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bitstream.checksum.fl_str_mv |
5e89a1613ddc8510c6576f4b23a78973 e39d27027a6cc9cb039ad269a5db8e34 16d99cded8c7e4bd1eacc2e5f2e451e9 33cbfae5e551ca4e4b6533b2ca142792 260a4016b5f87355a0f6a92ec3ce5164 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFPE - Universidade Federal de Pernambuco (UFPE) |
repository.mail.fl_str_mv |
attena@ufpe.br |
_version_ |
1802310732472123392 |