A potential biofilm metabolite signature for caries activity - a pilot clinical study
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/149906 |
Resumo: | Background: This study’s aim was to compare the dental biofilm metabolite-profile of caries-active (N=11) or caries-free (N=4) children by gas chromatography-mass spectrometry (GC/MS) analyses. Methods: Samples collected after overnight fasting, with or without a previous glucose rinse, were combined for each child based on the caries status of the site, re-suspended in ethanol and analyzed by GC/MS. Results: Biofilm from caries-active sites exhibited a different chromatographic profile compared to caries-free sites. Qualitative and quantitative analysis suggested a special cluster of branched alcohols and esters present at substantially higher intensity in biofilms of caries-active sites. Conclusions: This pilot study indicates that there are metabolites present in the biofilm which have the potential to provide a characteristic metabolomics signature for caries activity. |
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Zandona, A. F.Soini, H. A.Novotny, M. V.Santiago, E.Eckert, George JosephPreisser, J. S.Benecha, H. K.Arthur, Rodrigo AlexZero, Domenick Thomas2016-11-26T02:19:38Z20152153-0769http://hdl.handle.net/10183/149906000988349Background: This study’s aim was to compare the dental biofilm metabolite-profile of caries-active (N=11) or caries-free (N=4) children by gas chromatography-mass spectrometry (GC/MS) analyses. Methods: Samples collected after overnight fasting, with or without a previous glucose rinse, were combined for each child based on the caries status of the site, re-suspended in ethanol and analyzed by GC/MS. Results: Biofilm from caries-active sites exhibited a different chromatographic profile compared to caries-free sites. Qualitative and quantitative analysis suggested a special cluster of branched alcohols and esters present at substantially higher intensity in biofilms of caries-active sites. Conclusions: This pilot study indicates that there are metabolites present in the biofilm which have the potential to provide a characteristic metabolomics signature for caries activity.application/pdfengMetabolomics : open access. Los AngelesCárie dentáriaCromatografia gasosaBiofilmesA potential biofilm metabolite signature for caries activity - a pilot clinical studyEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000988349.pdf000988349.pdfTexto completo (inglês)application/pdf1347451http://www.lume.ufrgs.br/bitstream/10183/149906/1/000988349.pdfeeeabce1c3097f7b8a7a51a4e2e478e5MD51TEXT000988349.pdf.txt000988349.pdf.txtExtracted Texttext/plain35135http://www.lume.ufrgs.br/bitstream/10183/149906/2/000988349.pdf.txte5201de12fc5bc049218cfe2efb677b5MD52THUMBNAIL000988349.pdf.jpg000988349.pdf.jpgGenerated Thumbnailimage/jpeg2030http://www.lume.ufrgs.br/bitstream/10183/149906/3/000988349.pdf.jpg36fa053c30627cb0a2f9510271931c22MD5310183/1499062021-09-18 04:44:03.802348oai:www.lume.ufrgs.br:10183/149906Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-09-18T07:44:03Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
A potential biofilm metabolite signature for caries activity - a pilot clinical study |
title |
A potential biofilm metabolite signature for caries activity - a pilot clinical study |
spellingShingle |
A potential biofilm metabolite signature for caries activity - a pilot clinical study Zandona, A. F. Cárie dentária Cromatografia gasosa Biofilmes |
title_short |
A potential biofilm metabolite signature for caries activity - a pilot clinical study |
title_full |
A potential biofilm metabolite signature for caries activity - a pilot clinical study |
title_fullStr |
A potential biofilm metabolite signature for caries activity - a pilot clinical study |
title_full_unstemmed |
A potential biofilm metabolite signature for caries activity - a pilot clinical study |
title_sort |
A potential biofilm metabolite signature for caries activity - a pilot clinical study |
author |
Zandona, A. F. |
author_facet |
Zandona, A. F. Soini, H. A. Novotny, M. V. Santiago, E. Eckert, George Joseph Preisser, J. S. Benecha, H. K. Arthur, Rodrigo Alex Zero, Domenick Thomas |
author_role |
author |
author2 |
Soini, H. A. Novotny, M. V. Santiago, E. Eckert, George Joseph Preisser, J. S. Benecha, H. K. Arthur, Rodrigo Alex Zero, Domenick Thomas |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Zandona, A. F. Soini, H. A. Novotny, M. V. Santiago, E. Eckert, George Joseph Preisser, J. S. Benecha, H. K. Arthur, Rodrigo Alex Zero, Domenick Thomas |
dc.subject.por.fl_str_mv |
Cárie dentária Cromatografia gasosa Biofilmes |
topic |
Cárie dentária Cromatografia gasosa Biofilmes |
description |
Background: This study’s aim was to compare the dental biofilm metabolite-profile of caries-active (N=11) or caries-free (N=4) children by gas chromatography-mass spectrometry (GC/MS) analyses. Methods: Samples collected after overnight fasting, with or without a previous glucose rinse, were combined for each child based on the caries status of the site, re-suspended in ethanol and analyzed by GC/MS. Results: Biofilm from caries-active sites exhibited a different chromatographic profile compared to caries-free sites. Qualitative and quantitative analysis suggested a special cluster of branched alcohols and esters present at substantially higher intensity in biofilms of caries-active sites. Conclusions: This pilot study indicates that there are metabolites present in the biofilm which have the potential to provide a characteristic metabolomics signature for caries activity. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2016-11-26T02:19:38Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/149906 |
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2153-0769 |
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000988349 |
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http://hdl.handle.net/10183/149906 |
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eng |
language |
eng |
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Metabolomics : open access. Los Angeles |
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openAccess |
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application/pdf |
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