A potential biofilm metabolite signature for caries activity - a pilot clinical study

• Autor(a) principal: Zandona, A. F.
• Data de Publicação: 2015
• Outros Autores: Soini, H. A., Novotny, M. V., Santiago, E., Eckert, George Joseph, Preisser, J. S., Benecha, H. K., Arthur, Rodrigo Alex, Zero, Domenick Thomas
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/149906
• topic: Cárie dentária; Cromatografia gasosa; Biofilmes

Background: This study’s aim was to compare the dental biofilm metabolite-profile of caries-active (N=11) or caries-free (N=4) children by gas chromatography-mass spectrometry (GC/MS) analyses. Methods: Samples collected after overnight fasting, with or without a previous glucose rinse, were combined for each child based on the caries status of the site, re-suspended in ethanol and analyzed by GC/MS. Results: Biofilm from caries-active sites exhibited a different chromatographic profile compared to caries-free sites. Qualitative and quantitative analysis suggested a special cluster of branched alcohols and esters present at substantially higher intensity in biofilms of caries-active sites. Conclusions: This pilot study indicates that there are metabolites present in the biofilm which have the potential to provide a characteristic metabolomics signature for caries activity.