Antifungal activity and stability of fluconazole emulsion containing ionic liquids explained by intermolecular interactions
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/253603 |
Resumo: | This research reports accelerated stability experiments, the evaluation of intermolecular interactions, and antifungal assays for fluconazole emulsions prepared using ultrasound (US) and magnetic stirring (MS) in the presence of ionic liquids derived from 1,n-(3-methylimidazolium-1- yl)alkane bromide ([CnMIM]Br; n = 12 or 16). The goals of the investigation are to quantify the stability, identify the forces that drive the formation and stability, and determine the antifungal activity of fluconazole-containing emulsions, and corroborate the data from our previous results that indicated that the emulsion based on [C16MIM]Br seemed to be more stable. In this study, accelerated stability experiments evidenced a considerable stability for the [C16MIM]Br emulsions at two temperatures (25 and 37 C)—the instability index increased in the following order: US40% < US20% < MS. The 1H NMR data showed that the ILs interacts differently with medium-chain triglycerides (MCT). Two distinct interaction mechanisms were also observed for [C12MIM]Br and [C16MIM]Br with fluconazole, in which the latter formed more compact mixed aggregates than the former. The result was corroborated by diffusion data, which showed that ILs suffered a decrease in diffusion in the presence of fluconazole. The antifungal assay showed that emulsions containing ILs displayed superior activity compared with fluconazole alone. The emulsions also showed potent activity in inhibiting a resistant species (C. glabrata—CG34) to FLZ. All emulsions showed weak irritant potential in HET-CAM assay. |
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Hennemann, Bruno LuísBender, Caroline RaquelMoleta, Guilherme ScalcãoCarvalho, Anderson RamosBazana, Luana Candice GenzFuentefria, Alexandre MeneghelloFrizzo, Clarissa Piccinin2023-01-12T04:56:15Z20221999-4923http://hdl.handle.net/10183/253603001159480This research reports accelerated stability experiments, the evaluation of intermolecular interactions, and antifungal assays for fluconazole emulsions prepared using ultrasound (US) and magnetic stirring (MS) in the presence of ionic liquids derived from 1,n-(3-methylimidazolium-1- yl)alkane bromide ([CnMIM]Br; n = 12 or 16). The goals of the investigation are to quantify the stability, identify the forces that drive the formation and stability, and determine the antifungal activity of fluconazole-containing emulsions, and corroborate the data from our previous results that indicated that the emulsion based on [C16MIM]Br seemed to be more stable. In this study, accelerated stability experiments evidenced a considerable stability for the [C16MIM]Br emulsions at two temperatures (25 and 37 C)—the instability index increased in the following order: US40% < US20% < MS. The 1H NMR data showed that the ILs interacts differently with medium-chain triglycerides (MCT). Two distinct interaction mechanisms were also observed for [C12MIM]Br and [C16MIM]Br with fluconazole, in which the latter formed more compact mixed aggregates than the former. The result was corroborated by diffusion data, which showed that ILs suffered a decrease in diffusion in the presence of fluconazole. The antifungal assay showed that emulsions containing ILs displayed superior activity compared with fluconazole alone. The emulsions also showed potent activity in inhibiting a resistant species (C. glabrata—CG34) to FLZ. All emulsions showed weak irritant potential in HET-CAM assay.application/pdfengPharmaceutics. Basel. Vol. 14, n. 4 (2022), 710, 18 p.AntifúngicosFluconazolLíquidos iônicosIonic liquidUltrasoundEmulsionFluconazoleIntermolecular interactionAntifungal activityAntifungal activity and stability of fluconazole emulsion containing ionic liquids explained by intermolecular interactionsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001159480.pdf.txt001159480.pdf.txtExtracted Texttext/plain73227http://www.lume.ufrgs.br/bitstream/10183/253603/2/001159480.pdf.txt523259f60e91b947919b74d1467ce7b1MD52ORIGINAL001159480.pdfTexto completo (inglês)application/pdf5308375http://www.lume.ufrgs.br/bitstream/10183/253603/1/001159480.pdf6d833a5e96ed4f18b2e4f2d75f15710bMD5110183/2536032023-01-13 06:03:36.993858oai:www.lume.ufrgs.br:10183/253603Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-01-13T08:03:36Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Antifungal activity and stability of fluconazole emulsion containing ionic liquids explained by intermolecular interactions |
title |
Antifungal activity and stability of fluconazole emulsion containing ionic liquids explained by intermolecular interactions |
spellingShingle |
Antifungal activity and stability of fluconazole emulsion containing ionic liquids explained by intermolecular interactions Hennemann, Bruno Luís Antifúngicos Fluconazol Líquidos iônicos Ionic liquid Ultrasound Emulsion Fluconazole Intermolecular interaction Antifungal activity |
title_short |
Antifungal activity and stability of fluconazole emulsion containing ionic liquids explained by intermolecular interactions |
title_full |
Antifungal activity and stability of fluconazole emulsion containing ionic liquids explained by intermolecular interactions |
title_fullStr |
Antifungal activity and stability of fluconazole emulsion containing ionic liquids explained by intermolecular interactions |
title_full_unstemmed |
Antifungal activity and stability of fluconazole emulsion containing ionic liquids explained by intermolecular interactions |
title_sort |
Antifungal activity and stability of fluconazole emulsion containing ionic liquids explained by intermolecular interactions |
author |
Hennemann, Bruno Luís |
author_facet |
Hennemann, Bruno Luís Bender, Caroline Raquel Moleta, Guilherme Scalcão Carvalho, Anderson Ramos Bazana, Luana Candice Genz Fuentefria, Alexandre Meneghello Frizzo, Clarissa Piccinin |
author_role |
author |
author2 |
Bender, Caroline Raquel Moleta, Guilherme Scalcão Carvalho, Anderson Ramos Bazana, Luana Candice Genz Fuentefria, Alexandre Meneghello Frizzo, Clarissa Piccinin |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Hennemann, Bruno Luís Bender, Caroline Raquel Moleta, Guilherme Scalcão Carvalho, Anderson Ramos Bazana, Luana Candice Genz Fuentefria, Alexandre Meneghello Frizzo, Clarissa Piccinin |
dc.subject.por.fl_str_mv |
Antifúngicos Fluconazol Líquidos iônicos |
topic |
Antifúngicos Fluconazol Líquidos iônicos Ionic liquid Ultrasound Emulsion Fluconazole Intermolecular interaction Antifungal activity |
dc.subject.eng.fl_str_mv |
Ionic liquid Ultrasound Emulsion Fluconazole Intermolecular interaction Antifungal activity |
description |
This research reports accelerated stability experiments, the evaluation of intermolecular interactions, and antifungal assays for fluconazole emulsions prepared using ultrasound (US) and magnetic stirring (MS) in the presence of ionic liquids derived from 1,n-(3-methylimidazolium-1- yl)alkane bromide ([CnMIM]Br; n = 12 or 16). The goals of the investigation are to quantify the stability, identify the forces that drive the formation and stability, and determine the antifungal activity of fluconazole-containing emulsions, and corroborate the data from our previous results that indicated that the emulsion based on [C16MIM]Br seemed to be more stable. In this study, accelerated stability experiments evidenced a considerable stability for the [C16MIM]Br emulsions at two temperatures (25 and 37 C)—the instability index increased in the following order: US40% < US20% < MS. The 1H NMR data showed that the ILs interacts differently with medium-chain triglycerides (MCT). Two distinct interaction mechanisms were also observed for [C12MIM]Br and [C16MIM]Br with fluconazole, in which the latter formed more compact mixed aggregates than the former. The result was corroborated by diffusion data, which showed that ILs suffered a decrease in diffusion in the presence of fluconazole. The antifungal assay showed that emulsions containing ILs displayed superior activity compared with fluconazole alone. The emulsions also showed potent activity in inhibiting a resistant species (C. glabrata—CG34) to FLZ. All emulsions showed weak irritant potential in HET-CAM assay. |
publishDate |
2022 |
dc.date.issued.fl_str_mv |
2022 |
dc.date.accessioned.fl_str_mv |
2023-01-12T04:56:15Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/253603 |
dc.identifier.issn.pt_BR.fl_str_mv |
1999-4923 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001159480 |
identifier_str_mv |
1999-4923 001159480 |
url |
http://hdl.handle.net/10183/253603 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Pharmaceutics. Basel. Vol. 14, n. 4 (2022), 710, 18 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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