Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT

Detalhes bibliográficos
Autor(a) principal: Dakic, Vanja
Data de Publicação: 2017
Outros Autores: Nascimento, Juliana Minardi, Sartore, Rafaela Costa, Maciel, Renata de Moraes, Araújo, Dráulio Barros de, Ribeiro, Sidarta Tollendal Gomes, Martins-de-Souza, Daniel, Rehen, Stevens K.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/24038
Resumo: Dimethyltryptamines are entheogenic serotonin-like molecules present in traditional Amerindian medicine recently associated with cognitive gains, antidepressant effects, and changes in brain areas related to attention. Legal restrictions and the lack of adequate experimental models have limited the understanding of how such substances impact human brain metabolism. Here we used shotgun mass spectrometry to explore proteomic differences induced by 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) on human cerebral organoids. Out of the 6,728 identified proteins, 934 were found differentially expressed in 5-MeO-DMT-treated cerebral organoids. In silico analysis reinforced previously reported anti-inflammatory actions of 5-MeO-DMT and revealed modulatory effects on proteins associated with long-term potentiation, the formation of dendritic spines, including those involved in cellular protrusion formation, microtubule dynamics, and cytoskeletal reorganization. Our data offer the first insight about molecular alterations caused by 5-MeO-DMT in human cerebral organoids.
id UFRN_42362a03c13718725b1b795b3fef60b0
oai_identifier_str oai:https://repositorio.ufrn.br:123456789/24038
network_acronym_str UFRN
network_name_str Repositório Institucional da UFRN
repository_id_str
spelling Dakic, VanjaNascimento, Juliana MinardiSartore, Rafaela CostaMaciel, Renata de MoraesAraújo, Dráulio Barros deRibeiro, Sidarta Tollendal GomesMartins-de-Souza, DanielRehen, Stevens K.2017-10-11T15:26:28Z2017-10-11T15:26:28Z2017-10-09DAKIC, V. et al. Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT. Scietific reports, [s. l.], v. 7, p.12863, out./2017.https://repositorio.ufrn.br/jspui/handle/123456789/2403810.1038/s41598-017-12779-5eng5-MeO-DMTNeural stem cellsCellular neuroscienceNeuroscienceProteomicsShort term changes in the proteome of human cerebral organoids induced by 5-MeO-DMTinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDimethyltryptamines are entheogenic serotonin-like molecules present in traditional Amerindian medicine recently associated with cognitive gains, antidepressant effects, and changes in brain areas related to attention. Legal restrictions and the lack of adequate experimental models have limited the understanding of how such substances impact human brain metabolism. Here we used shotgun mass spectrometry to explore proteomic differences induced by 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) on human cerebral organoids. Out of the 6,728 identified proteins, 934 were found differentially expressed in 5-MeO-DMT-treated cerebral organoids. In silico analysis reinforced previously reported anti-inflammatory actions of 5-MeO-DMT and revealed modulatory effects on proteins associated with long-term potentiation, the formation of dendritic spines, including those involved in cellular protrusion formation, microtubule dynamics, and cytoskeletal reorganization. Our data offer the first insight about molecular alterations caused by 5-MeO-DMT in human cerebral organoids.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALDraulioBarros_ICe_2017_Short term.pdfDraulioBarros_ICe_2017_Short term.pdfDraulioBarros_ICe_2017_Short termapplication/pdf4522397https://repositorio.ufrn.br/bitstream/123456789/24038/1/DraulioBarros_ICe_2017_Short%20term.pdf850c1015b0a011cd14d1d96137a2f5a7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/123456789/24038/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52TEXTDraulioBarros_ICe_2017_Short term.pdf.txtDraulioBarros_ICe_2017_Short term.pdf.txtExtracted texttext/plain57316https://repositorio.ufrn.br/bitstream/123456789/24038/5/DraulioBarros_ICe_2017_Short%20term.pdf.txt09d08bf07a9b9e4da2ca9d0b58b1c866MD55THUMBNAILDraulioBarros_ICe_2017_Short term.pdf.jpgDraulioBarros_ICe_2017_Short term.pdf.jpgIM Thumbnailimage/jpeg9538https://repositorio.ufrn.br/bitstream/123456789/24038/6/DraulioBarros_ICe_2017_Short%20term.pdf.jpgd6aee4e06e15669fd93a93c97531bb02MD56123456789/240382021-07-10 19:42:34.269oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-07-10T22:42:34Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT
title Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT
spellingShingle Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT
Dakic, Vanja
5-MeO-DMT
Neural stem cells
Cellular neuroscience
Neuroscience
Proteomics
title_short Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT
title_full Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT
title_fullStr Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT
title_full_unstemmed Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT
title_sort Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT
author Dakic, Vanja
author_facet Dakic, Vanja
Nascimento, Juliana Minardi
Sartore, Rafaela Costa
Maciel, Renata de Moraes
Araújo, Dráulio Barros de
Ribeiro, Sidarta Tollendal Gomes
Martins-de-Souza, Daniel
Rehen, Stevens K.
author_role author
author2 Nascimento, Juliana Minardi
Sartore, Rafaela Costa
Maciel, Renata de Moraes
Araújo, Dráulio Barros de
Ribeiro, Sidarta Tollendal Gomes
Martins-de-Souza, Daniel
Rehen, Stevens K.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Dakic, Vanja
Nascimento, Juliana Minardi
Sartore, Rafaela Costa
Maciel, Renata de Moraes
Araújo, Dráulio Barros de
Ribeiro, Sidarta Tollendal Gomes
Martins-de-Souza, Daniel
Rehen, Stevens K.
dc.subject.por.fl_str_mv 5-MeO-DMT
Neural stem cells
Cellular neuroscience
Neuroscience
Proteomics
topic 5-MeO-DMT
Neural stem cells
Cellular neuroscience
Neuroscience
Proteomics
description Dimethyltryptamines are entheogenic serotonin-like molecules present in traditional Amerindian medicine recently associated with cognitive gains, antidepressant effects, and changes in brain areas related to attention. Legal restrictions and the lack of adequate experimental models have limited the understanding of how such substances impact human brain metabolism. Here we used shotgun mass spectrometry to explore proteomic differences induced by 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) on human cerebral organoids. Out of the 6,728 identified proteins, 934 were found differentially expressed in 5-MeO-DMT-treated cerebral organoids. In silico analysis reinforced previously reported anti-inflammatory actions of 5-MeO-DMT and revealed modulatory effects on proteins associated with long-term potentiation, the formation of dendritic spines, including those involved in cellular protrusion formation, microtubule dynamics, and cytoskeletal reorganization. Our data offer the first insight about molecular alterations caused by 5-MeO-DMT in human cerebral organoids.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-10-11T15:26:28Z
dc.date.available.fl_str_mv 2017-10-11T15:26:28Z
dc.date.issued.fl_str_mv 2017-10-09
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DAKIC, V. et al. Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT. Scietific reports, [s. l.], v. 7, p.12863, out./2017.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/24038
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-017-12779-5
identifier_str_mv DAKIC, V. et al. Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT. Scietific reports, [s. l.], v. 7, p.12863, out./2017.
10.1038/s41598-017-12779-5
url https://repositorio.ufrn.br/jspui/handle/123456789/24038
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
bitstream.url.fl_str_mv https://repositorio.ufrn.br/bitstream/123456789/24038/1/DraulioBarros_ICe_2017_Short%20term.pdf
https://repositorio.ufrn.br/bitstream/123456789/24038/2/license.txt
https://repositorio.ufrn.br/bitstream/123456789/24038/5/DraulioBarros_ICe_2017_Short%20term.pdf.txt
https://repositorio.ufrn.br/bitstream/123456789/24038/6/DraulioBarros_ICe_2017_Short%20term.pdf.jpg
bitstream.checksum.fl_str_mv 850c1015b0a011cd14d1d96137a2f5a7
8a4605be74aa9ea9d79846c1fba20a33
09d08bf07a9b9e4da2ca9d0b58b1c866
d6aee4e06e15669fd93a93c97531bb02
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)
repository.mail.fl_str_mv
_version_ 1814833047074242560

Registros relacionados