Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT

Detalhes bibliográficos
Autor(a) principal: Winne, Jessica
Data de Publicação: 2020
Outros Autores: Boerner, Barbara C., Malfatti, Thawann, Brisa, Elis, Doerl, Jhulimar, Nogueira, Ingrid, Leão, Emelie Katarina Svahn, Leão, Richardson Naves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/28384
Resumo: Salicylate intoxication is a cause of tinnitus and comorbidly associated with anxiety in humans. In a previous work, we showed that salicylate induces anxiety-like behavior and hippocampal type 2 theta oscillations (theta2) in mice. Here we investigate if the anxiogenic effect of salicylate is dependent on age and previous tinnitus experience. We also tested whether a single dose of DMT can prevent this effect. Using microwire electrode arrays, we recorded local field potential in young (4-5- month-old) and old (11-13-month-old) mice to study the electrophysiological effect of tinnitus in the ventral hippocampus (vHipp) and medial prefrontal cortex (mPFC) in an open field arena and elevated plus maze 1h after salicylate (300mg/kg) injection. We found that anxiety-like behavior and increase in theta2 oscillations (4-6 Hz), following salicylate pre-treatment, only occurs in young (normal hearing) mice. We also show that theta2 and slow gamma oscillations increase in the vHipp and mPFC in a complementary manner during anxiety tests in the presence of salicylate. Finally, we show that pre-treating mice with a single dose of the hallucinogenic 5-MeO-DMT prevents anxiety-like behavior and the increase in theta2 and slow gamma oscillations after salicylate injection in normal hearing young mice. This work further support the hypothesis that anxiety-like behavior after salicylate injection is triggered by tinnitus and require normal hearing. Moreover, our results show that hallucinogenic compounds can be effective in treating tinnitus-related anxiety.
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spelling Winne, JessicaBoerner, Barbara C.Malfatti, ThawannBrisa, ElisDoerl, JhulimarNogueira, IngridLeão, Emelie Katarina SvahnLeão, Richardson Naves2020-01-24T19:32:55Z2020-01-24T19:32:55Z2020-01-08WINNE, J.; BOERNER, B. C.; MALFATTI, T.; BRISA, E.; DOERL, J.; NOGUEIRA, I.; LEÃO, K. E.; LEÃO, R. N. Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT. Exp Neurol., [s. l.], v. 326, p. 113175, jan. 2020.https://repositorio.ufrn.br/jspui/handle/123456789/2838410.1016/j.expneurol.2020.1131755-MeO-DMT and anxietyTinnitusType 2 thetaVentral hippocampusAnxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMTinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSalicylate intoxication is a cause of tinnitus and comorbidly associated with anxiety in humans. In a previous work, we showed that salicylate induces anxiety-like behavior and hippocampal type 2 theta oscillations (theta2) in mice. Here we investigate if the anxiogenic effect of salicylate is dependent on age and previous tinnitus experience. We also tested whether a single dose of DMT can prevent this effect. Using microwire electrode arrays, we recorded local field potential in young (4-5- month-old) and old (11-13-month-old) mice to study the electrophysiological effect of tinnitus in the ventral hippocampus (vHipp) and medial prefrontal cortex (mPFC) in an open field arena and elevated plus maze 1h after salicylate (300mg/kg) injection. We found that anxiety-like behavior and increase in theta2 oscillations (4-6 Hz), following salicylate pre-treatment, only occurs in young (normal hearing) mice. We also show that theta2 and slow gamma oscillations increase in the vHipp and mPFC in a complementary manner during anxiety tests in the presence of salicylate. Finally, we show that pre-treating mice with a single dose of the hallucinogenic 5-MeO-DMT prevents anxiety-like behavior and the increase in theta2 and slow gamma oscillations after salicylate injection in normal hearing young mice. This work further support the hypothesis that anxiety-like behavior after salicylate injection is triggered by tinnitus and require normal hearing. Moreover, our results show that hallucinogenic compounds can be effective in treating tinnitus-related anxiety.engreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/28384/2/license.txte9597aa2854d128fd968be5edc8a28d9MD52TEXTRichardsonLeao_ICe_2020_Anxiety-like behavior.pdf.txtRichardsonLeao_ICe_2020_Anxiety-like behavior.pdf.txtExtracted texttext/plain79726https://repositorio.ufrn.br/bitstream/123456789/28384/3/RichardsonLeao_ICe_2020_Anxiety-like%20behavior.pdf.txta4f8b420211a5e57d2e91402daa505aaMD53THUMBNAILRichardsonLeao_ICe_2020_Anxiety-like behavior.pdf.jpgRichardsonLeao_ICe_2020_Anxiety-like behavior.pdf.jpgGenerated Thumbnailimage/jpeg1687https://repositorio.ufrn.br/bitstream/123456789/28384/4/RichardsonLeao_ICe_2020_Anxiety-like%20behavior.pdf.jpgb7a80e2d621f1ecf21d0d91dc2e6920aMD54123456789/283842023-02-06 15:34:34.792oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2023-02-06T18:34:34Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT
title Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT
spellingShingle Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT
Winne, Jessica
5-MeO-DMT and anxiety
Tinnitus
Type 2 theta
Ventral hippocampus
title_short Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT
title_full Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT
title_fullStr Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT
title_full_unstemmed Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT
title_sort Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT
author Winne, Jessica
author_facet Winne, Jessica
Boerner, Barbara C.
Malfatti, Thawann
Brisa, Elis
Doerl, Jhulimar
Nogueira, Ingrid
Leão, Emelie Katarina Svahn
Leão, Richardson Naves
author_role author
author2 Boerner, Barbara C.
Malfatti, Thawann
Brisa, Elis
Doerl, Jhulimar
Nogueira, Ingrid
Leão, Emelie Katarina Svahn
Leão, Richardson Naves
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Winne, Jessica
Boerner, Barbara C.
Malfatti, Thawann
Brisa, Elis
Doerl, Jhulimar
Nogueira, Ingrid
Leão, Emelie Katarina Svahn
Leão, Richardson Naves
dc.subject.por.fl_str_mv 5-MeO-DMT and anxiety
Tinnitus
Type 2 theta
Ventral hippocampus
topic 5-MeO-DMT and anxiety
Tinnitus
Type 2 theta
Ventral hippocampus
description Salicylate intoxication is a cause of tinnitus and comorbidly associated with anxiety in humans. In a previous work, we showed that salicylate induces anxiety-like behavior and hippocampal type 2 theta oscillations (theta2) in mice. Here we investigate if the anxiogenic effect of salicylate is dependent on age and previous tinnitus experience. We also tested whether a single dose of DMT can prevent this effect. Using microwire electrode arrays, we recorded local field potential in young (4-5- month-old) and old (11-13-month-old) mice to study the electrophysiological effect of tinnitus in the ventral hippocampus (vHipp) and medial prefrontal cortex (mPFC) in an open field arena and elevated plus maze 1h after salicylate (300mg/kg) injection. We found that anxiety-like behavior and increase in theta2 oscillations (4-6 Hz), following salicylate pre-treatment, only occurs in young (normal hearing) mice. We also show that theta2 and slow gamma oscillations increase in the vHipp and mPFC in a complementary manner during anxiety tests in the presence of salicylate. Finally, we show that pre-treating mice with a single dose of the hallucinogenic 5-MeO-DMT prevents anxiety-like behavior and the increase in theta2 and slow gamma oscillations after salicylate injection in normal hearing young mice. This work further support the hypothesis that anxiety-like behavior after salicylate injection is triggered by tinnitus and require normal hearing. Moreover, our results show that hallucinogenic compounds can be effective in treating tinnitus-related anxiety.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-01-24T19:32:55Z
dc.date.available.fl_str_mv 2020-01-24T19:32:55Z
dc.date.issued.fl_str_mv 2020-01-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv WINNE, J.; BOERNER, B. C.; MALFATTI, T.; BRISA, E.; DOERL, J.; NOGUEIRA, I.; LEÃO, K. E.; LEÃO, R. N. Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT. Exp Neurol., [s. l.], v. 326, p. 113175, jan. 2020.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/28384
dc.identifier.doi.none.fl_str_mv 10.1016/j.expneurol.2020.113175
identifier_str_mv WINNE, J.; BOERNER, B. C.; MALFATTI, T.; BRISA, E.; DOERL, J.; NOGUEIRA, I.; LEÃO, K. E.; LEÃO, R. N. Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT. Exp Neurol., [s. l.], v. 326, p. 113175, jan. 2020.
10.1016/j.expneurol.2020.113175
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