Partition of α-lactoalbumin and β-lactoglobulin by cloud point extraction

Detalhes bibliográficos
Autor(a) principal: Monteiro, Paulo Sérgio
Data de Publicação: 2008
Outros Autores: Coimbra, Jane Sélia dos Reis, Minim, Luis Antonio, Oliveira, Juraci Alves de, Silva, Luis Henrique Mendes da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: https://doi.org/10.1016/j.jchromb.2008.03.028
http://www.locus.ufv.br/handle/123456789/19623
Resumo: This work aimed to study the partition of cheese whey proteins α-lactoalbumin and β-lactoglobulin using aqueous two-phase system by applying the cloud point extraction technique. The cloud point temperatures were determined under different concentrations of copolymer and salt. The system providing the best protein separation conditions was 20 mass% of copolymer PE61 and potassium phosphate salt solution of 100 mM, at pH 7. The protein α-lactoalbumin remained preferentially in the aqueous phase and the β-lactoglobulin was transferred to the copolymer phase.
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spelling Monteiro, Paulo SérgioCoimbra, Jane Sélia dos ReisMinim, Luis AntonioOliveira, Juraci Alves deSilva, Luis Henrique Mendes da2018-05-16T14:11:41Z2018-05-16T14:11:41Z2008-04-041570-0232https://doi.org/10.1016/j.jchromb.2008.03.028http://www.locus.ufv.br/handle/123456789/19623This work aimed to study the partition of cheese whey proteins α-lactoalbumin and β-lactoglobulin using aqueous two-phase system by applying the cloud point extraction technique. The cloud point temperatures were determined under different concentrations of copolymer and salt. The system providing the best protein separation conditions was 20 mass% of copolymer PE61 and potassium phosphate salt solution of 100 mM, at pH 7. The protein α-lactoalbumin remained preferentially in the aqueous phase and the β-lactoglobulin was transferred to the copolymer phase.engJournal of Chromatography BVolume 867, Issue 2, Pages 189-193, 15 May 2008Elsevier Ltd.info:eu-repo/semantics/openAccessAqueous two-phase systemsCloud pointα-Lactoalbuminβ-LactoglobulinPartition of α-lactoalbumin and β-lactoglobulin by cloud point extractioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALartigo.pdfartigo.pdfTexto completoapplication/pdf373104https://locus.ufv.br//bitstream/123456789/19623/1/artigo.pdfbc7d7f9bb902b4e1464c0e068f2a853aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://locus.ufv.br//bitstream/123456789/19623/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52THUMBNAILartigo.pdf.jpgartigo.pdf.jpgIM Thumbnailimage/jpeg5364https://locus.ufv.br//bitstream/123456789/19623/3/artigo.pdf.jpg6d0e925b38640e7a8fe1133e5e90b19bMD53123456789/196232018-05-16 23:00:33.485oai:locus.ufv.br:123456789/19623Tk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452018-05-17T02:00:33LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.en.fl_str_mv Partition of α-lactoalbumin and β-lactoglobulin by cloud point extraction
title Partition of α-lactoalbumin and β-lactoglobulin by cloud point extraction
spellingShingle Partition of α-lactoalbumin and β-lactoglobulin by cloud point extraction
Monteiro, Paulo Sérgio
Aqueous two-phase systems
Cloud point
α-Lactoalbumin
β-Lactoglobulin
title_short Partition of α-lactoalbumin and β-lactoglobulin by cloud point extraction
title_full Partition of α-lactoalbumin and β-lactoglobulin by cloud point extraction
title_fullStr Partition of α-lactoalbumin and β-lactoglobulin by cloud point extraction
title_full_unstemmed Partition of α-lactoalbumin and β-lactoglobulin by cloud point extraction
title_sort Partition of α-lactoalbumin and β-lactoglobulin by cloud point extraction
author Monteiro, Paulo Sérgio
author_facet Monteiro, Paulo Sérgio
Coimbra, Jane Sélia dos Reis
Minim, Luis Antonio
Oliveira, Juraci Alves de
Silva, Luis Henrique Mendes da
author_role author
author2 Coimbra, Jane Sélia dos Reis
Minim, Luis Antonio
Oliveira, Juraci Alves de
Silva, Luis Henrique Mendes da
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Monteiro, Paulo Sérgio
Coimbra, Jane Sélia dos Reis
Minim, Luis Antonio
Oliveira, Juraci Alves de
Silva, Luis Henrique Mendes da
dc.subject.pt-BR.fl_str_mv Aqueous two-phase systems
Cloud point
α-Lactoalbumin
β-Lactoglobulin
topic Aqueous two-phase systems
Cloud point
α-Lactoalbumin
β-Lactoglobulin
description This work aimed to study the partition of cheese whey proteins α-lactoalbumin and β-lactoglobulin using aqueous two-phase system by applying the cloud point extraction technique. The cloud point temperatures were determined under different concentrations of copolymer and salt. The system providing the best protein separation conditions was 20 mass% of copolymer PE61 and potassium phosphate salt solution of 100 mM, at pH 7. The protein α-lactoalbumin remained preferentially in the aqueous phase and the β-lactoglobulin was transferred to the copolymer phase.
publishDate 2008
dc.date.issued.fl_str_mv 2008-04-04
dc.date.accessioned.fl_str_mv 2018-05-16T14:11:41Z
dc.date.available.fl_str_mv 2018-05-16T14:11:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1016/j.jchromb.2008.03.028
http://www.locus.ufv.br/handle/123456789/19623
dc.identifier.issn.none.fl_str_mv 1570-0232
identifier_str_mv 1570-0232
url https://doi.org/10.1016/j.jchromb.2008.03.028
http://www.locus.ufv.br/handle/123456789/19623
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartofseries.pt-BR.fl_str_mv Volume 867, Issue 2, Pages 189-193, 15 May 2008
dc.rights.driver.fl_str_mv Elsevier Ltd.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Elsevier Ltd.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Journal of Chromatography B
publisher.none.fl_str_mv Journal of Chromatography B
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
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instname_str Universidade Federal de Viçosa (UFV)
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institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
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