Quantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?ncia
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
Texto Completo: | https://tede.ufrrj.br/jspui/handle/tede/369 |
Resumo: | A?a? (Euterpe oleraceae Mart) is a fruit with a high energetic and nutritional value that presents high content of phenolic compounds, mainly anthocyanin pigments directly related to its color. Over the past few years the interest on these pigments has been intensified as many scientific studies demonstrate that the anthocyanins and their aglycones are bioactive compounds and they can be related to disease prevention, mainly those caused by cellular oxidation. The food industry has a great interest in technological innovations that attend for of the requirements of the consumer market. The use of membrane processes, particularly microfiltration, represents an option that justifies the investments and researches on this subject. In general, these processes are conducted at room temperature without heat or phase change, preserving the sensory and nutritional quality of the products in addition to the reduction in the energetic costs involved in the process. The objective of this work was to evaluate the content of the main anthocyanins, cyanidin-3-O-glicoside and cyanidin-3-Orutenoside, using high performance liquid chromatography (HPLC) by using standards previously isolated from freeze-dried a?ai samples, in the clarification of a?ai juice by microfiltration. In order to decrease the juice viscosity and the pulp content, consequently increasing the efficiency of the process it was introduced two steps before the microfiltration, an enzymatic hydrolysis followed by the juice centrifugation. The use of HPLC allowed the isolation of analytic standards from natural sources with a high purity level more than 97% allowing the quantification of each anthocyanin and its yield in each step of the process. The main anthocyanins isolated were conclusively identified by HPLC and mass spectrometry confirming data reported by other researches about the presence of anthocyanins cyanidin-3- O-glucoside and cyanidin-3-O-rutenoside as major anthocyanins of a?a?. This technique can also contribute to the characterization of new matrix , allowing the confirmation of previously published data. The obtained results showed that it is possible to use new matrix to isolate standards and to characterize other samples with confidence and economy. Regarding the microfiltration evaluation, it was possible to quantify the levels of cyanidin-3-O-glicoside and cyanidin-3-O-rutenoside in the feed and permeate fractions, respectively, 37 and 97 ?g / 100g for the feed and 15 and 54 ?g / 100g for the permeate. |
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Cabral, Lourdes Maria Correa628.646.967-20http://lattes.cnpq.br/7249897840870537636674287http://lattes.cnpq.br/0719676140304035Gouv?a, Ana Cristina Miranda Senna2016-04-28T14:54:28Z2011-04-132010-02-23GOUV?A, Ana Cristina Miranda Senna. Quantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?ncia. 2010. 76 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2010.https://tede.ufrrj.br/jspui/handle/tede/369A?a? (Euterpe oleraceae Mart) is a fruit with a high energetic and nutritional value that presents high content of phenolic compounds, mainly anthocyanin pigments directly related to its color. Over the past few years the interest on these pigments has been intensified as many scientific studies demonstrate that the anthocyanins and their aglycones are bioactive compounds and they can be related to disease prevention, mainly those caused by cellular oxidation. The food industry has a great interest in technological innovations that attend for of the requirements of the consumer market. The use of membrane processes, particularly microfiltration, represents an option that justifies the investments and researches on this subject. In general, these processes are conducted at room temperature without heat or phase change, preserving the sensory and nutritional quality of the products in addition to the reduction in the energetic costs involved in the process. The objective of this work was to evaluate the content of the main anthocyanins, cyanidin-3-O-glicoside and cyanidin-3-Orutenoside, using high performance liquid chromatography (HPLC) by using standards previously isolated from freeze-dried a?ai samples, in the clarification of a?ai juice by microfiltration. In order to decrease the juice viscosity and the pulp content, consequently increasing the efficiency of the process it was introduced two steps before the microfiltration, an enzymatic hydrolysis followed by the juice centrifugation. The use of HPLC allowed the isolation of analytic standards from natural sources with a high purity level more than 97% allowing the quantification of each anthocyanin and its yield in each step of the process. The main anthocyanins isolated were conclusively identified by HPLC and mass spectrometry confirming data reported by other researches about the presence of anthocyanins cyanidin-3- O-glucoside and cyanidin-3-O-rutenoside as major anthocyanins of a?a?. This technique can also contribute to the characterization of new matrix , allowing the confirmation of previously published data. The obtained results showed that it is possible to use new matrix to isolate standards and to characterize other samples with confidence and economy. Regarding the microfiltration evaluation, it was possible to quantify the levels of cyanidin-3-O-glicoside and cyanidin-3-O-rutenoside in the feed and permeate fractions, respectively, 37 and 97 ?g / 100g for the feed and 15 and 54 ?g / 100g for the permeate.O a?a? (Euterpe oleraceae Mart) ? um fruto com alto valor energ?tico e nutricional que possui um elevado teor de compostos fen?licos, principalmente de pigmentos antoci?nicos diretamente relacionados ? sua colora??o. Nos ?ltimos anos, o interesse por esses pigmentos se intensificou uma vez que v?rios estudos cient?ficos t?m demonstrado que as antocianinas e suas respectivas agliconas s?o compostos bioativos e que podem estar relacionados ? preven??o de doen?as, principalmente aquelas causadas por oxida??o celular. A ind?stria de alimentos apresenta forte interesse em inova??es tecnol?gicas que permitam atender ?s necessidades e anseios do mercado consumidor. A aplica??o dos processos com membranas, especificamente a microfiltra??o, representa uma alternativa que justifica o grande interesse em investimentos e pesquisas. Em geral, s?o processos que ocorrem ? temperatura ambiente sem que haja mudan?a de fase ou utiliza??o de calor. Desta forma, permitem a manuten??o das propriedades sensoriais e nutricionais dos produtos, al?m de representar uma economia nos custos energ?ticos envolvidos no processo. O objetivo deste trabalho foi avaliar o teor das antocianinas majorit?rias, cianidina-O-3-glicos?deo e cianidina-O-3-rutenos?deo, por Cromatografia L?quida de Alta Efici?ncia (CLAE), utilizando padr?es previamente isolados de amostras de a?a? liofilizadas, durante clarifica??o de suco de a?a? por microfiltra??o. Para diminuir a viscosidade e o teor de polpa do suco e, consequentemente, aumentar a efici?ncia do processo, foram introduzidas uma etapa de hidr?lise enzim?tica e outra de centrifuga??o do suco antes da microfiltra??o possibilitando a quantifica??o de cada antocianina e o seu rendimento em cada etapa do processo. As antocianinas majorit?rias isoladas foram conclusivamente identificadas por CLAE e espectrometria de massas, confirmando as informa??es obtidas na literatura quanto ? presen?a das antocianinas cianidina-3-O-glicos?deo e cianidina-3-O-rutenos?deo como majorit?rias do a?a?. Esta t?cnica tamb?m pode contribuir para caracteriza??o de outras matrizes, sendo poss?vel a confirma??o de dados previamente publicados. Com os resultados obtidos, conclui-se tamb?m ser poss?vel a utiliza??o de outras matrizes para isolar padr?es e assim caracterizar outras amostras com confian?a e economia. Em rela??o ? microfiltra??o, foi poss?vel quantificar os teores de cianidina-3-O-glicos?deo e cianidina-3-O-rutenos?deo nas correntes de alimenta??o e permeado que apresentaram respectivamente, 37 e 97 ?g / 100g na alimenta??o e 15 e 54 ?g / 100g no permeado.Made available in DSpace on 2016-04-28T14:54:28Z (GMT). No. of bitstreams: 1 2010 - Ana Cristina Miranda Senna Gouv?a.pdf: 2088585 bytes, checksum: c834197678127c00451580f31ab99bbd (MD5) Previous issue date: 2010-02-23application/pdfhttps://tede.ufrrj.br/retrieve/13647/2010%20-%20Ana%20Cristina%20Miranda%20Senna%20Gouv%c3%aaa.pdf.jpghttps://tede.ufrrj.br/retrieve/18022/2010%20-%20Ana%20Cristina%20Miranda%20Senna%20Gouv%c3%aaa.pdf.jpghttps://tede.ufrrj.br/retrieve/24366/2010%20-%20Ana%20Cristina%20Miranda%20Senna%20Gouv%c3%aaa.pdf.jpghttps://tede.ufrrj.br/retrieve/30733/2010%20-%20Ana%20Cristina%20Miranda%20Senna%20Gouv%c3%aaa.pdf.jpghttps://tede.ufrrj.br/retrieve/37081/2010%20-%20Ana%20Cristina%20Miranda%20Senna%20Gouv%c3%aaa.pdf.jpghttps://tede.ufrrj.br/retrieve/43483/2010%20-%20Ana%20Cristina%20Miranda%20Senna%20Gouv%c3%aaa.pdf.jpghttps://tede.ufrrj.br/retrieve/49831/2010%20-%20Ana%20Cristina%20Miranda%20Senna%20Gouv%c3%aaa.pdf.jpghttps://tede.ufrrj.br/retrieve/56296/2010%20-%20Ana%20Cristina%20Miranda%20Senna%20Gouv%c3%aaa.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncia e Tecnologia de AlimentosUFRRJBrasilInstituto de Tecnologiapadr?es de antocianinasCLAEa?a?microfiltra??ostandards of anthocyaninsHPLCa?a?microfiltrationCi?ncia e Tecnologia de AlimentosQuantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?nciaQuantification of the major anthocyanins of a?a? by high performance liquid chromatographyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFRRJinstname:Universidade Federal Rural do Rio de Janeiro (UFRRJ)instacron:UFRRJTHUMBNAIL2010 - Ana Cristina Miranda Senna Gouv?a.pdf.jpg2010 - Ana Cristina Miranda Senna Gouv?a.pdf.jpgimage/jpeg4014http://localhost:8080/tede/bitstream/tede/369/17/2010+-+Ana+Cristina+Miranda+Senna+Gouv%C3%AAa.pdf.jpg35294b0bfa9783b4c23923134bf98188MD517TEXT2010 - Ana Cristina Miranda Senna Gouv?a.pdf.txt2010 - Ana Cristina Miranda Senna Gouv?a.pdf.txttext/plain142427http://localhost:8080/tede/bitstream/tede/369/16/2010+-+Ana+Cristina+Miranda+Senna+Gouv%C3%AAa.pdf.txt97d78a0096607ddb09776aa2573ee4a7MD516ORIGINAL2010 - Ana Cristina Miranda Senna Gouv?a.pdf2010 - Ana Cristina Miranda Senna Gouv?a.pdfapplication/pdf2100123http://localhost:8080/tede/bitstream/tede/369/1/2010+-+Ana+Cristina+Miranda+Senna+Gouv%C3%AAa.pdf02fda249d102895f9fa97e3fc5e58e57MD51tede/3692022-06-24 13:13:39.339oai:localhost:tede/369Biblioteca Digital de Teses e Dissertaçõeshttps://tede.ufrrj.br/PUBhttps://tede.ufrrj.br/oai/requestbibliot@ufrrj.br||bibliot@ufrrj.bropendoar:2022-06-24T16:13:39Biblioteca Digital de Teses e Dissertações da UFRRJ - Universidade Federal Rural do Rio de Janeiro (UFRRJ)false |
dc.title.por.fl_str_mv |
Quantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?ncia |
dc.title.alternative.eng.fl_str_mv |
Quantification of the major anthocyanins of a?a? by high performance liquid chromatography |
title |
Quantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?ncia |
spellingShingle |
Quantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?ncia Gouv?a, Ana Cristina Miranda Senna padr?es de antocianinas CLAE a?a? microfiltra??o standards of anthocyanins HPLC a?a? microfiltration Ci?ncia e Tecnologia de Alimentos |
title_short |
Quantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?ncia |
title_full |
Quantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?ncia |
title_fullStr |
Quantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?ncia |
title_full_unstemmed |
Quantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?ncia |
title_sort |
Quantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?ncia |
author |
Gouv?a, Ana Cristina Miranda Senna |
author_facet |
Gouv?a, Ana Cristina Miranda Senna |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Cabral, Lourdes Maria Correa |
dc.contributor.advisor1ID.fl_str_mv |
628.646.967-20 |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7249897840870537 |
dc.contributor.authorID.fl_str_mv |
636674287 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0719676140304035 |
dc.contributor.author.fl_str_mv |
Gouv?a, Ana Cristina Miranda Senna |
contributor_str_mv |
Cabral, Lourdes Maria Correa |
dc.subject.por.fl_str_mv |
padr?es de antocianinas CLAE a?a? microfiltra??o |
topic |
padr?es de antocianinas CLAE a?a? microfiltra??o standards of anthocyanins HPLC a?a? microfiltration Ci?ncia e Tecnologia de Alimentos |
dc.subject.eng.fl_str_mv |
standards of anthocyanins HPLC a?a? microfiltration |
dc.subject.cnpq.fl_str_mv |
Ci?ncia e Tecnologia de Alimentos |
description |
A?a? (Euterpe oleraceae Mart) is a fruit with a high energetic and nutritional value that presents high content of phenolic compounds, mainly anthocyanin pigments directly related to its color. Over the past few years the interest on these pigments has been intensified as many scientific studies demonstrate that the anthocyanins and their aglycones are bioactive compounds and they can be related to disease prevention, mainly those caused by cellular oxidation. The food industry has a great interest in technological innovations that attend for of the requirements of the consumer market. The use of membrane processes, particularly microfiltration, represents an option that justifies the investments and researches on this subject. In general, these processes are conducted at room temperature without heat or phase change, preserving the sensory and nutritional quality of the products in addition to the reduction in the energetic costs involved in the process. The objective of this work was to evaluate the content of the main anthocyanins, cyanidin-3-O-glicoside and cyanidin-3-Orutenoside, using high performance liquid chromatography (HPLC) by using standards previously isolated from freeze-dried a?ai samples, in the clarification of a?ai juice by microfiltration. In order to decrease the juice viscosity and the pulp content, consequently increasing the efficiency of the process it was introduced two steps before the microfiltration, an enzymatic hydrolysis followed by the juice centrifugation. The use of HPLC allowed the isolation of analytic standards from natural sources with a high purity level more than 97% allowing the quantification of each anthocyanin and its yield in each step of the process. The main anthocyanins isolated were conclusively identified by HPLC and mass spectrometry confirming data reported by other researches about the presence of anthocyanins cyanidin-3- O-glucoside and cyanidin-3-O-rutenoside as major anthocyanins of a?a?. This technique can also contribute to the characterization of new matrix , allowing the confirmation of previously published data. The obtained results showed that it is possible to use new matrix to isolate standards and to characterize other samples with confidence and economy. Regarding the microfiltration evaluation, it was possible to quantify the levels of cyanidin-3-O-glicoside and cyanidin-3-O-rutenoside in the feed and permeate fractions, respectively, 37 and 97 ?g / 100g for the feed and 15 and 54 ?g / 100g for the permeate. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-02-23 |
dc.date.available.fl_str_mv |
2011-04-13 |
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2016-04-28T14:54:28Z |
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GOUV?A, Ana Cristina Miranda Senna. Quantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?ncia. 2010. 76 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2010. |
dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/tede/369 |
identifier_str_mv |
GOUV?A, Ana Cristina Miranda Senna. Quantifica??o das antocianinas majorit?rias do a?a? por cromatografia l?quida de alta efici?ncia. 2010. 76 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos) - Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, 2010. |
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