Bacillus cereus: Isolamento, Contagem e Detec??o de enterotoxinas por PCR em amostras de caf? torrado e mo?do comercializado na cidade do Rio de Janeiro.
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| Data de Publicação: | 2011 |
| Tipo de documento: | Tese |
| Idioma: | por |
| Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFRRJ |
| Texto Completo: | https://tede.ufrrj.br/jspui/handle/jspui/3836 |
Resumo: | Coffee is one of the most appreciated drinks in the world. Coffee grounds are obtained from the fruit of a small plant that belongs to the genus Coffea. Coffea arabica and Coffea canephora robusta are the two species of highest commercial importance. They are more commonly known as arabica and robusta, respectively. Two-thirds of Coffea arabica plants are grown in South and Central America and Eastern Africa (the place of origin for this coffee species). Contamination with microorganisms has been a major factor affecting coffee quality, due to the method of harvest adopted in Brazil. Brazilian harvests are based on mixing fruits collected from the ground with those that fall on cloths. As the Bacillus cereus bacterium frequently uses soil as an environmental reservoir, it is easily capable of becoming a contaminant. In the current study, microbiological analysis and polymerase chain reaction (PCR) analysis of the toxicogenic profile of isolated strains were used to evaluate B. cereus contamination in commercial samples of ground and roasted coffee in the city of Rio de Janeiro. The results revealed a significant frequency of these enterotoxin genes in isolated strains, which were: The HBL (57%); HBL C (71%); HBL D (64%); The NHE (50%); NHE B (100%) ; NHE C (64%). Strains of B.thuringiensis were also found in 44% of the samples. |
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Abrantes, Shirley de Mello Pereira512.535.507-00http://lattes.cnpq.br/1296030772265453Amendoeira, Maria Regina ReisFreitas, Sidinea Cordeiro deZamith, Helena Pereira da SilvaLuchese, Rosa Helena517.467.525-72http://lattes.cnpq.br/2287133994409786Souza, Cyllene de Matos Ornelas da Cunha Corr?a de2020-08-21T00:56:40Z2011-08-01SOUZA, Cyllene de Matos Ornelas da Cunha Corr?a de. Bacillus cereus: Isolamento, Contagem e Detec??o de enterotoxinas por PCR em amostras de caf? torrado e mo?do comercializado na cidade do Rio de Janeiro.. 2011. [91 f.]. Tese( Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos) - Universidade Federal Rural do Rio de Janeiro, Serop?dica.https://tede.ufrrj.br/jspui/handle/jspui/3836Coffee is one of the most appreciated drinks in the world. Coffee grounds are obtained from the fruit of a small plant that belongs to the genus Coffea. Coffea arabica and Coffea canephora robusta are the two species of highest commercial importance. They are more commonly known as arabica and robusta, respectively. Two-thirds of Coffea arabica plants are grown in South and Central America and Eastern Africa (the place of origin for this coffee species). Contamination with microorganisms has been a major factor affecting coffee quality, due to the method of harvest adopted in Brazil. Brazilian harvests are based on mixing fruits collected from the ground with those that fall on cloths. As the Bacillus cereus bacterium frequently uses soil as an environmental reservoir, it is easily capable of becoming a contaminant. In the current study, microbiological analysis and polymerase chain reaction (PCR) analysis of the toxicogenic profile of isolated strains were used to evaluate B. cereus contamination in commercial samples of ground and roasted coffee in the city of Rio de Janeiro. The results revealed a significant frequency of these enterotoxin genes in isolated strains, which were: The HBL (57%); HBL C (71%); HBL D (64%); The NHE (50%); NHE B (100%) ; NHE C (64%). Strains of B.thuringiensis were also found in 44% of the samples.O caf? ? uma das bebidas mais consumidas e apreciadas no mundo. O gr?o de caf? ? obtido da fruta da planta, um arbusto pequeno, pertencente ao g?nero Coffea. Duas esp?cies t?m import?ncia comercial: Coffea arabica e Coffea canephora robusta; conhecidas como arabica e robusta. Cerca de dois ter?os da esp?cie Coffea arabica cresce principalmente na Am?rica do Sul, Am?rica Central e Leste da ?frica (origem deste caf?). A incid?ncia de microrganismos tem sido um dos principais fatores envolvidos na qualidade do caf?, principalmente na modalidade de colheita e preparo adotada no Brasil, isto ?, a colheita baseada na mistura de frutos derri?ados no ch?o com os derri?ados no pano. A bact?ria Bacillus cereus, que utiliza freq?entemente o solo e o meio ambiente como reservat?rio pode ser adicionado a esta lista de microrganismos. No presente estudo, avaliouse a contamina??o de B. cereus em amostras de caf? torrado e mo?do comercializado na cidade do Rio de janeiro, atrav?s de an?lises microbiol?gicas e an?lise do perfil toxig?nico das cepas isoladas atrav?s da Rea??o em Cadeia da Polimerase (PCR), considerando-se os complexos HBL e NHE. Os resultados revelaram elevada freq??ncia desses genes de enterotoxinas nas cepas isoladas, que foram: HBL A (57%); HBL C (71%); HBL D (64%); NHE A (50 %); NHE B (100%); NHE C (64%). Cepas de B. thuringiensis foram encontradas em 44% das amostras analisadas.Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2020-08-21T00:56:40Z No. of bitstreams: 1 2011 - Cyllene de Matos Ornelas da Cunha Corr?a de Souza.pdf: 954640 bytes, checksum: acab6a0de78f1ddb78e58879f2020864 (MD5)Made available in DSpace on 2020-08-21T00:56:40Z (GMT). No. of bitstreams: 1 2011 - Cyllene de Matos Ornelas da Cunha Corr?a de Souza.pdf: 954640 bytes, checksum: acab6a0de78f1ddb78e58879f2020864 (MD5) Previous issue date: 2011-08-01application/pdfhttps://tede.ufrrj.br/retrieve/61712/2011%20-%20Cyllene%20de%20Matos%20Ornelas%20da%20Cunha%20Corr%c3%aaa%20de%20Souza.pdf.jpgporUniversidade Federal Rural do Rio de JaneiroPrograma de P?s-Gradua??o em Ci?ncia e Tecnologia de AlimentosUFRRJBrasilInstituto de Tecnologia- ABIC ? ASSOCIA??O BRASILEIRA DA IND?STRIA DE CAF?. Dispon?vel em: www.abic.com.br>. Acesso em 18 Jul. 2008 - AFONSO J?NIOR, P.C.; CORR?A, P.C.; GONELI, A.L.D.; SILVA, F.S. Contribui??o das etapas do pr?-processamento para a qualidade do caf?. Revista Brasileira de Armazenamento, Vi?osa, Especial Caf?, n. 8, p. 46-53, 2004. - ANG?LICO, C.L. Qualidade do caf? (Coffea ar?bica L.) em diferentes est?dios de matura??o e submetido a cinco tempos de ensacamentos antes da secagem. 2008.149f. Disserta??o (Mestrado em Ci?ncias dos Alimentos)- Universidade Federal de Lavras, lavras, MG. - ALVES, E.; CASTRO, H.A. Fungos associados ao caf? (Coffea arabica L.) nas fases de pr? e p?s-colheita em lavouras da regi?o de Lavras. Summa Phytopathologica, Jaboticabal, v. 24, n. 1, p. 4-7, 1998 - ALVES, S.B. Controle Microbiano de Insetos. 2aed. Piracicaba: FEALQ, 1998. - ANDERSSON, A.; R?NNER, U.; GRANUM, P. E. What problems does the food industry have the spore-forming pathogens Bacillus cereus and Clostridium perfringens? International Journal of Food Microbiology, v. 28, n. 2, p. 145-155, dec. 1995 - ANDERSSON, A., GRANUM, P. E., & RONNER, U. (1998). The adhesion of Bacillus cereus spores to epithelial cells might be an additional virulence mechanism. International Journal of Food Microbiology, 39, 93-99. - APHA (American Public Health Association). Microbiological monitoring of the food processing environment. In: Compendium of methods for the microbiological examination of foods. 3th. ed., Washington. Chap. 3, p.51: 75. 2001 - ARNESEN, L. P. S., FAGERLUND, A. & GRANUM, P. E. (2008). From soil to gut: Bacillus cereus and its food poisoning toxins. Acedido em 23-07-2008, http://www3.interscience.wiley.com/journal/120085025/abstract?CRETRY=1&SRETRY=0 54 - ATHIE, I.; PAULA, D.C. de. Insetos de gr?os armazenados ? aspectos biol?gicos e identifica??o. 2. ed. Sao Paulo: Varela. 2002. 244p. - AZEVEDO, J. L. Engenharia Gen?tica aplicada ao controle microbiano de insetos. In: ALVES, S.B. Controle Microbiano de Insetos. 2.ed. Piracicaba: FEALQ, 1998. - BACHHIL, V. N.; JAISWAL, T. N. Bacillus cereus in meats: incidence, prevalence and enterotoxigenicity. J. Food Sci. Technol., v. 25, n. 6, p. 371-372, Nov./ Dec. 1988 - BAPTIST J M, MANDEL,M & GHERMA R L.1978. Comparative zone eletrophoresis of enzymes in the Genus Bacillus. Int J Syst Bacteriol. 28:229-244. - BALBANI,A.P.S.; BUTUGAN.O. Contamina??o biol?gica de alimentos. REVIS?O E ENSAIO REVIEW AND ESSAY REVISI?N Y ENSAIO 2001. - BARRIOS, B.E.B. Caracteriza??o f?sica, qu?mica, microbiol?gica e sensorial de caf?s (Coffea arabica L.) da regi?o Alto Rio Grande ? Sul de Minas Gerais. 2001. 72 p.: il. Disserta??o (Mestrado em Ci?ncia dos Alimentos). Universidade Federal de Lavras, Lavras. - BARROS, V.R.M.; PANETTA, J.C., MIGUEL, O. Ocorr?ncia e n?veis de Bacillus cereus no leite em p? integral comercializado na capital do estado de S?o Paulo, Brasil ? 1987/1988. Rev. Educ. Contin. CRMV-SP, v.4, p.45-51, 2001. - BECKER, H.; SCHALLER, G; WIESE, W.; TERPLAN, G. Bacillus cereus in infant foods and dried milk products. International Journal of Food Microbiology, v. 23, n. 1, p. 1-15, set. 1994. - BEECHER, D.J.; MACMILLAN, J.D. Characterization of the components of hemolysin BL from Bacillus cereus. Infectionand Immunity, v.59,n.5, p.1778-1784, 1991. - BEECHER, D. J.; WONG, A. C. L. Identification and analysis of the antigens detected by two commercial Bacillus cereus diarrhoeal enterotoxin immunoassay kits. Applied and Environmental Microbiology, v. 60, n. 12, p. 4614-4616, dec. 1994. - BEECHER, D.J. Enterotoxic activity of hemolysin BL from Bacillus cereus. Infection and Immunity, v.63, n.11, p.4423-4428, 1995. 55 - BENEDICT, R. C.; PARTRIDGE, T.; WELLS, D.; BUCHANAN, R. L. Bacillus cereus: aerobic growth kinetics. Journal of Food Protection, v. 56, n. 3, p. 211-214, mar. 1993. - BERG, R. W.; SANDINE, W. E. Activation of bacterial spores. A review. Journal Milk Food Technology, v.33, p.435-441. 1970. - BENNETT, R. W.; BELAY, M. Bacillus cereus. In: DOWNES, F. P.; ITO, K. Compendium of methods for the microbiological examination of foods. 4th, American Public Health Association, cap. 32, p. 311-316, 2001. - BERNHARD, K.; JARRETT, P.; MEADOWS, M. et al. Natural isolates of Bacillus thuringiensis: worldwide distribution, characterization, and activity against insect pests. Journal of Invertebrate Pathology, Orlando, v.70, p.59-68, 1997. - BIOCHER, J. C. e BUSTA, F. F. Bacterial spore resistence to acid. Food Technology, v.37, p.87-89. 1983. - BHUNIA, A. K. (2007). Bacillus cereus and Bacillus anthracis. In A. K. Bhunia (Eds.) Foodborne Microbial Pathogens: Mechanisms and Pathogenesis;(1? Edi??o). (276). Springer. - BITANCOURT, A.A. O tratamento das cerejas de caf? para melhorar a bebida. O Biol?gico, S?o Paulo, 2001, vol. 23, n. 1, p. 1-11. - BOZZA, A.; TRALAMAZZA, S.M.; REYNAUD, D.T.; GABARDO, J.; VALASKI, J.C.; MARANGONI, P.B.; PIMENTEL, I.C. Isolamento de fungos associados a gr?os de caf? cv. Iapar 59 de origem de solo e ?rvore em diferentes tempos de colheita. Ci?ncia e Tecnologia de Alimentos, Campinas, v. 29, n. 3, p. 529-534, 2009. - BORGE, G. A.; SKEIE, M.; SORHAUG, T.; LANGSRUD, T.; GRANUM, P. E. Growth and toxin profiles of Bacillus cereus isolated from different food sources. International Journal of Food Microbiology, v. 69, n. 3, p. 237-246, sept. 2001. - BUGNO.A.;BUZZO.A.A.;NAKAMURA,C.T.;PEREIRA,T.C.;MATOS,D.;PINTO.T.J.A. Avalia??o da contamina??o microbiana em drogas vegetais. Revista Brasileira de 56 Ci?ncias Farmac?uticas Brazilian Journal of Pharmaceutical Sciences vol. 41, n. 4, out./dez., 2005. - BURDON K.L. 1946. J Bacterio. 52:665-678. - BURTON, G.R.W.; ENGELKIRK, P.G. Microbiologia para as ci?ncias da sa?de. Guanabara Koogan. Rio de Janeiro: 7? ed, 2005. - BRASIL, Resolu??o-RDC n? 12, de 2 de janeiro de 2001, da Ag?ncia Nacional de Vigil?ncia Sanit?ria, do Minist?rio da Sa?de - Aprova Regulamento T?cnico sobre padr?es microbiol?gicos para alimentos. Di?rio Oficial [da] Rep?blica Federativa do Brasil, Bras?lia, 10 de janeiro de 2001. - BRASIL, Resolu??o RDC n? 175 , de 08 de julho de 2003, da Ag?ncia Nacional de Vigil?ncia Sanit?ria, do Minist?rio da Sa?de - Aprova o regulamento t?cnico de Avalia??o de Mat?rias Macrosc?picas e Microsc?picas prejudiciais a Sa?de Humana em alimentos embalados. Di?rio Oficial [da] Rep?blica Federativa do Brasil, Bras?lia, 10 de julho de 2003. - BRASIL. Portaria n? 377, de 26 de abril de 1999. Institui normas b?sicas de alimentos. Di?rio Oficial da Uni?o [da] Rep?blica Federativa do Brasil, Bras?lia, Poder Executivo, Bras?lia, DF, 29 abr. 1999. - BRASIL. Portaria n ? 451, de 19 de setembro de 1997. Institui Princ?pios Gerais para o Estabelecimento de Crit?rios e Padr?es Microbiol?gicos para Alimentos. Di?rio Oficial da Uni?o [da] Rep?blica Federativa do Brasil, Bras?lia, Poder Executivo, Bras?lia, 19 de setembro de 1997. - BRAVO, A.; GILL, S. S.; SOBERON, M. Bacillus Thuringiensis: Mecanisms and use In:______. Comprehensive molecular inset science. Amsterdam:Elsevier BV, 2005. p.175- 206. - CARVALHO, V.D.; CHALFOUN, S.M. Aspectos qualitativos do caf?. Informe Agropecu?rio, Belo Horizonte, v. 11, n. 126, p. 79-92, 1985. 57 - CARVALHO,V.D; Chagas,S.J.R.;Souza,S.M.C. Fatores que afetam a qualidade do caf?. Informe Agropecu?rio, Belo Horizonte, 2005, v.18, n.187, p.5-20. - CARVALHO, V.D. 1997. Cafeicultura empresarial: produtividade e qualidade - qualidade do caf?. - COSTA, F.N, OLIVEIRA,E.M, BORGES,R.G. Ocorr?ncia de bact?rias da esp?cie Bacillus cereus em amostras de leite em p? integral obtidas no com?rcio varejista da cidade de S?o Lu?s, MA. Hig. aliment;18(121): 104-107, jun. 2004. - CHALFOUN, S.M.; CARVALHO, V.D. de. Microflora associada a frutos e gr?os de caf? de diferentes locais, tipos de colheita e diferentes etapas do preparo. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 15., 1989, Maring?, PR. Resumos... Rio de Janeiro: MIC/IBC, 1989, p. 17-21. - CHALFOUN, S.M.; PARIZZI, F.C. Fungos toxig?nicos e micotoxinas em caf?. In: BOR?M, F.M. P?s-colheita do caf?. Lavras: UFLA, 2008. cap. 14, p. 512-54. - CLAUS D & BERKELEY RCW. Genus Bacillus. In: Sneath PHA, Mair NS, Sharpe ME &Holt JG. Bergey?s Manual of Systematic Bacteriology. Baltimore: Williams & Wilkins; 1986, vol. 2, 1104-1139p. - CLAVEL, T., CARLIN, F., LAIRON, D., NGUYEN-THE, C., & SCHMITT, P. (2004). Survival of Bacillus cereus spores and vegetative cells in acid media simulating human stomach. Journal of Applied Microbiology, 97, 214-219. - CHRISTIANSSON, A. Enterotoxin production in milk by Bacillus cereus: a comparison of methods for toxin detection. Netherlands Milk and Dairy Journal, v. 47, n. 2, p. 79-87, 1993. - CHRISTIANSSON, A. The toxicology of Bacillus cereus. Bull. Int. Dairy Fed., n.275, p.30-35, 1992. 58 - CHILCOTT, C. N.; WIGLEY, P. J. Isolation and toxicity of Bacillus thuringiensis from soil and insect habitats in New Zealand. Journal of Invertebrate Pathology, v. 61, p. 244-247, 1993. - COPPING, L. G.; MENN, J. J. Review biopesticides: a review of their action, applications and efficacy. Pest management science, Sussex, v. 56, n. 8, p. 651-676, 2000. - COSENTINO, S.; MULARGIA, A.F.; PISANO, B. et al. Incidence and biochemical characteristics of Bacillus flora in Sardinian dairy products. Int. J. Food Microbiol., v.38, p.235-238, 1997. - DAMGAARD, P.H. Natural occurrence and dispersal of Bacillus thuringiensis in the environment. In: CHARLES, J.F.; DEL?CLUSE, A.; NIELSEN-LE ROUX, C. Entomopathogenic bacteria: from laboratory to field application. Netherlands: Kluwer Academic Publishers, 2000. p.23-40. - DE MAAGD, R.; BRAVO, A.; BERRY, C.; CRICKMORE, N.; SCHNEPF, H. E. Structure, diversity and evolution of protein toxins from spore-forming entomopathogenic bacteria. Annual Review Genetics, Palo Alto, v. 37, p. 409-433, 2003. - DELAZARi, I. Aspectos microbiol?gicos ligados a seguran?a e qualidade da carca?a de aves. In: Semana Acad?mica Veterin?ria, 8., 1998, S?o Paulo. Anais. S?o Paulo: 1998. p.71-77. - DOYLE, M. P. Bacillus cereus. Food Technology, Chicago, v.42, n.4, p.199-200, 1988 - DROBNIEWSKI, F. A. Bacillus cereus and related species. Clinical Microbiology Reviews, v. 6, n. 4, p. 324-338, oct. 1993. - EDWARDS, P.R, EWING, W.H. Identification of Enterobacteriaceae 3ed. Minneapolis: Burgess Publishing Co, 1972. - EHLING-SCHULZ, M., FRICKER, M. & SCHERER, S. (2004). Bacillus cereus, the causative agent of an emetic type of food-borne illness. 59 - EMBRAPA ? CAF?. Dispon?vel em:< http://www22.sede.embrapa.br/cafe/outros/relatorio_gestao.htm>.Acesso em 24 Jul.2008. - EDWARDS K.; JONHSTONE C.; THOMPSON C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucl. Acid Res., 19- 1349, 1991. - EVANS, J. A.; RUSSEL, S. L.; JAMES, C.; CORRY, J. E. L Microbial contamination of food refrigeration equipament. Journal of Food Engineering, v.62, n. 3, p. 225-232, may 2004. - ERLICH, H.A. PCR: technology: principles and applications for DNA amplification. New York/Oxford: Oxford University Press. 1992. - FERMANIAN, C.; LAPEYRE, C.; FR?MY, J. M.; CLAISSE, M. Production of diarrhoeal toxin by selected strains of Bacillus cereus. International Journal of Food Microbiology, v. 30, n. 3, p. 345-358, july 1996. - FERMANIAN, C. F.; WONG, A. C. L. Improved in vitro detection of hemolysin BL from Bacillus cereus. Int. J. Food Microbiol., v. 57, n. 2, p. 1-8, 2000. - FORSYTHE, S. J. Microbiologia da seguran?a alimentar. Porto Alegre: Artmed, 2002. - FRANCO, G. M. B.; Landgraf, M. Microbiologia dos Alimentos. S?o Paulo.: Editora Atheneu, 1996, cap.4, p. 33-81. - FRANCO, B. D. G. M.; LANDGRAF, M. Microrganismos patog?nicos de import?ncia em alimentos. In: FRANCO, B. D. G. M.; LANDGRAF, M. Microbiologia dos alimentos. S?o Paulo, Rio de Janeiro, Ribeir?o Preto, Belo Horizonte: Atheneu, 2006. cap. 4, p. 41-43. - FRITZE, D. (2004). Taxonomy of the Genus Bacillus and related genera: the aerobic endospore-forming bacteria. Acessada em 12/08/2009 http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2004.94.11.1245?cookieSet=1. - GALLO, D; NAKANO, O.; SILVEIRA NETO, S.; BAPTISTA, G.C.; BERTI FILHO, E.; PARRA, J.R.P.; ZUCCHI, R.A.; ALVES, S.B.; VENDRAMIM, J.D.; MARCHINI, L.C.; LOPES, J.R.S.; OMOTO, S. Entomologia Agr?cola. Piracicaba: FEALQ, 2002. 920p 60 - GILBERT, R. J. Bacillus cereus gastroenteritis. In: RIEMANN, H.; BRYAN, L. F., eds. Foodborne Infections and intoxications. 2 ed. New York: Academic Press, 1979. p. 495- 518. - GUINEBRETIERE, M.H.; NGUYEN-THE, C. Sources of Bacillus cereus contamination in a pasteurised zucchini pur?e processing line, differentiated by two PCR-based methods. FEMS Microbiology Ecology, v.43, n.2, p.207-215, 2003. - GLARE, T.R.; O?CALLAGHAM,M. Bacillus thuringiensis: biology, ecology and safaty. Chichester: John Wiley, 2000. - GOEPFERT, J. M.; SPIRA, W. M.; KIM, H. U. Bacillus cereus: food poisoning organism. A review. Journal of Milk and Food Technology, v. 35, n. 4, p. 213-226, 1972. - GOMES.L.P.;RODRIGUES M.M.; SOARES.G.; BARONI.F.A.;SOUZA.M.M.S. Bacillus cereus em amostras de doces industrializados comercializados por ambulantes nos munic?pios de serop?dica e itagua? ? Rj. Rev. Univ. Rural, S?r. Ci. Vida. Serop?dica, RJ, EDUR, v. 24, n.2, Jul.-Dez., p.181-184, 2004. - GORDON R E, HAYNES W C & PANG C H N. 1973. The genus Bacillus. Agriculture Handbook n? 427. U.S. Department of Agriculture, Washington,D.C. - GORE, H.M.; SPIRA, W.M.; KIM, H.U. Real-time molecular beacon NASBA reveals HBLc expression from Bacillus spp. In milk . Biochem. Bioph. Res. Com., v. 311, p. 386- 390, 2003. - GRANUM, P.E. Bacillus cereus and its toxins. Journal of Applied Bacteriology, 1994, v.76, p.61S-66S. - GRANUM, P. E. Bacillus cereus. In: DOYLE, M. P., BEUCHAT, L.R. MONTVILLE, T.J. (Eds.), Food Microbiology. Fundamentals and Frontiers. Washington: ASM Press, 1997. p. 327?336. 61 - GRANUM, P. E, BAIRD-PARKER, T. Bacillus species. In: LUND, B. M.; BAIRDPARKER, T. C.; GOULD, G. W. The microbiological safety and quality of food. Gaithersburg, Maryland: Aspen Publishers, Inc. 2000. Vol. II. Chap. 39, p. 1029-1039. - GRANUM, P. E.; BRYNESTAD, S.; KRAMER, J.M. Analysis of enterotoxin production by Bacillus cereus from dairy products, food poisoning incidents and non-gastrointestinal infections. International Journal of Food Microbiology, v. 17, n. 4, p. 269-279, feb. 1993 - GRANUM, P. E.; LUND, T. Bacillus cereus and its food poisoning toxins. FEMS Microbiology Letters, v. 157, n. 2, p. 223-228, dec. 1997. - Granum, P. E., O? Sullivan, K., & Lund, T. (1999). The sequence of the non-haemolytic enterotoxin operon from Bacillus cereus. FEMS microbiology letters, 177, 225-229. - GRIFFITHS, M. W. Toxin production by psyhrotrophic Bacillus spp. present in milk. Journal of Food Protection, v. 53, n. 9, p. 790-792, sep. 1990. - GRIFFITHS, M. W. Bacillus cereus in liquid milk and other milk products. Bulletin of the International Dairy Federation, Brussells, n. 275, p.36-39, 1992. - HABIB, M.E.M.; ANDRADE, C.F.S. BACT?RIAS ENTOMOPATOG?NICAS. IN: Controle Microbiano de Insetos. Editado Por S.B. Alves. 2. Ed. Fealq, Piracicaba, 1998. p. 383-446. - HANSEN, B. M.; HENDRIKSEN, N. B. Detection of enterotoxin Bacillus cereus and Bacillus thuringiensis strains by PCR analysis. Applied and Environmental Microbiology, v. 67, n. 1, p. 185-189, jan. 2001. - HANSEN BM, DAMGAARD PH, EILENBERG J, & PEDERSEN JC (1998) Molecular and phenotypic characterization of Bacillus thuringiensis isolated from leaves and insects. J Invertebr Pathol, 71: 106?114. - HANSEN BM, DAMGAARD PH, EILENBERG J, & PEDERSEN JC (1996) Bacillus thuringiensis, ecology and environmental effects of its use for microbial pest control 62 (Environmental Project No. 316). Copenhagen, Denmark, Ministry of Environment and Energy, Danish Environmental Protection Agency. - HARRIS H & HOPKINSON D A. 1976. Handbook of enzyme electrophoresis in human genetics. Elsevier Science Publish, New York. - HARRIS, H. & HOPKINSON, D.A. Handbook of enzyme electrophoresis in human genetics. Amsterdam: North- Holland, 1976. - HARWOOD. CR & CUTTING S.M. Molecular Biological Methods for Bacillus. Wiley e Sons Ltda, West Sussex, England; 1990. 65p. - HEALTH PROTECTION BRANCH. Guidelines for the general cleanliness of food: an Overview (Hpb). Ottawa, Ontario, 1999. 9p. - HEINRICHS, A.H. et al. Molecular cloning and characterization of the HBLA gene encoding the B component of hemolysin BL from Bacillus cereus. Journal Bacteriology, v.175, p.6760- 6766, 1993. - HOFTE, H.; WHITELEY, H.R. Inseticidal Crystal Proteins of Bacillus thuringiensis. Microbiological Review, Washington, v. 53, n. 2, p. 242-255, 1989. - HUSMARK, U.; SIK, U. R. Forces involved in adhesion of Bacillus cereus spores to solid surfaces under different environmental conditions. The Journal of Applied Bacteriology, v. 69, n. 4, p. 557-562, oct. 1990. - ILLY, A.; VIANI, R. Espresso coffee: the chemistry of quality. San Diego: Academic Press, 1995. 253 p. - JOHNSON, K. M. Bacillus cereus foodborne illness - an update. Journal of Food Protection, v. 47, n. 2, p. 145-153, feb. 1983. - JOHNSON, K. M.; NELSON, C. L.; BUSTA, F. F. Influence of temperature on germination and growth of spores of emetic and diarrheal strains of Bacillus cereus in a broth medium and in rice. Journal of Food Science, Chicago, v. 48, n. 1, p. 286-287, 1983 63 - J??SKEL?INEN, E. (2008). Assessment and control of Bacillus cereus emetic toxin in food. Department of Applied Chemistry and Microbiology - Division Microbiology. Helsinki, University of Helsinki: 74. - JAY, J. M. (2005). Bacillus cereus gastroenteritis. In J. M. Jay (Eds.) Modern Food Microbiology, (6? Edi??o). (720). Springer. - KNIEHL, E., BECKER, A. AND FORSTER, D. H. (2003). Pseudo-outbreak of toxigenic Bacillus cereus isolated from stools of three patients with diarrhoea after oral administration of a probiotic medication. Journal of Hospital Infection 55, 33-38. - KOTIRANTA, A.; LOUNATMAA, K., HAAPASALO, M. Epidemiology and pathogenesis of Bacillus cereus infections. Microbes and Infection, v. 2, n.2, p. 189-198, feb. 2000. - KUSKE, C. R., BANTON, K. L., ADORADA, D. L., STARK, P. C., HILL, K. K. & JACKSON, P. J. (1998). Small-Scale DNA Sample Preparation Method for Field PCR Detection of Microbial Cells and Spores in Soil. Appl. Environ. Microbiol., 64(7), 2463- 2472. - LAGO.N.C.M.R.;ROSSI.O.D.;VIDAL MARTINS.; AMARAL.L.A. Ocorr?ncia de Bacillus cereus em leite integral e capacidade enterotoxig?nica das cepas isoladas. Arq. Bras. Med. Vet. Zootec., v.59, n.6, p.1563-1569, 2007. - LI, CARREL, J. J.; ELLAR, D. J. Cristal estructure of insecticide delta-endotoxin from Bacillus thuringiensis at 2,5 A resolution. Nature, London, v. 353, n. 6.347, p.815-821, 1991. - LINDBACK, T., FAGERLUND, A., RODLANDT, M. S., & GRANUM, P. E. (2004). Characterization of the Bacillus cereus NHE enterotoxin. Microbiology, 150, 3959-3967 - LUND, T.; GRANUM, P.E. Characterisation of a nonhaemolytic enterotoxin complex from Bacillus cereus isolated after a food borne outbreak. FEMS Microbiology Letters, v.141, n.2-3, p.151-156, 1996. 64 - LUTHY P., WOLFERSBERGER, M.G. Pathogenisis of Bacillus thuringiensis toxin. In CHARLES J.F., DELECLUSE A., NIELSEN-LE ROUX, C. (Eds.) Entomopathogenic bacteria: from laboratory to field application. Kluwer Academic Publishers, 2000. p.167-180. - MALORNY, B. et al. Standardization of diagnostic PCR for detection of foodborne pathogens. Int. J. Food Microbiol., Amsterdam, v. 83, n. 1, p. 39-48, 2003. - M?NTYNEN, V.; LINDSTR?M, K. A Rapid PCR-Based DNA Test for Enterotoxic Bacillus cereus. Applied and Environmental Microbiology, Washington, v.64, n.5, p. 1634-1639, May 1998. - MARTINS. H. M, MARTINS. M. L, BERNADO, F. Caracteriza??o microbiol?gica e detec??o de aflatoxina e de ocratoxina A em gr?os de caf? torrados, comercializados em Portugal. 2004, Revista de Ci?ncias Agr?rias. Lisboa, Portugal. - MARTIN, P.A.; TRAVERS, R.S. Worldwide abundance and distribution of Bacillus thuringiensis isolates. Applied and Environmental Microbiology, Massachusetts, v.55, n.10, p.2437-2442, 1989. - MEADOWS MP, ELLIS DJ, BUTT J, JARRETT P, & BURGES HD (1992) Distribution, frequency and diversity of Bacillus thuringiensis in an animal feed mill. Appl Environ Microbiol, 58(4): 1344?1350. - MINNAARD, J.; HUMEN, M.; P?REZ, P. F. Bacillus cereus exocellular factors on human intestinal epithelial cells. Journal of Food Protection, v. 64, n. 10, p. 1535-1541, oct. 2001 - MONNERAT, R; BRAVO A. Prote?nas bioinseticidas produzidas pela bact?ria Bacillus thuringiensis: modo de a??o e resist?ncia. In: MELO IS, AZEVEDO JL, (eds). Controle Biol?gico. Jaguari?na: Embrapa Meio Ambiente. 2000. p.163. - NOTERMANS, S.; BATT, C. A. A risk assessment approach for food-borne Bacillus cereus and its toxins. Journal of Applied Microbiology, Readin, v. 84, p. 51S-61S, 1998 (Supplement Symposium). 65 - ODOMERU, J. A.; TONER, A. K.; MUCKLE, C. A.; GRIFFITHS, M. W.; LYNCH, J. A. Detection of Bacillus cereus diarrheal enterotoxin in raw and pasteurized milk. Journal of Food Protection, Des Moines, v. 30, n.11, p.1391-1393, 1997. - PELCZAR J.M; CHAN S.C.E; KRIEG R.N. Microbiologia: conceitos e aplica??es. 2.ed. S?o Paulo: Editora Afiliada, 1996. v. 2, p. 306-311. - PEREIRA, M. S.; OLIVEIRA, L. A. T.; FRANCO, R. M. C.; PRADO, J. C. A. Contagem, isolamento e identifica??o de Bacillus cereus em condimentos preparados, utilizados em embutido c?rneo (mortadela). Revista Brasileira de Ci?ncia Veterin?ria, v. 6, n. 3, p. 137- 140, set.-dez. 1999. - PIELAAT, A., WIJNANDS, L. M., TAKUMI, K., NAUTA, M. J., & VAN LEUSDEN, F. M. (2006). The fate of Bacillus cereus in gastro-intestinal tract: National Institute for Public Health and Environment. - PRIEST, F. G. Systematics and ecology of Bacillus. In: SONESHEIN, A. L., HOCH, J. A.; LOSIK, R. (ed.). Bacillus subtilis and other Gram-positive bacteria. Washington, DC: American Society for Microbiology, 1993. p. 3-16. - PR?SS, B. M.; DIETRICH, R.; NIBLER, B.; M?RTLBAUER, E.; SCHERER, S. The hemolytic enterotoxin HBL is broadly distributed among species of the Bacillus cereus group. Applied and Environmental Microbiology, v. 65, n. 12, p. 5436-5442, dec. 1999. - RABINOVITCH, L; VICENTE MMA; GUAYCUR?S TV; FREITAS JPGV & MESQUITA RP.. Avalia??o da incid?ncia e da toxicidade de amostras de Bacillus cereus em diferentes classes de alimentos comercializados e consumidos no Estado do Rio de Janeiro.Mem Inst Oswaldo Cruz , 1985, 80(1):1-9. - RADHIKA, B.; PADMAPRIYA, B. P.; CHANDRASHEKAR, A.; KESHAVA, N. and VARADAJ, M. C. Detection of Bacillus cereus in foods by colony hibridization using PCR-generated probe and characterization of isolates for toxins by PCR. International Journal of Food Microbiology, V. 74, p. 131-138, 2002. 66 - RYAN, P.A. et al. Molecular cloning and characterization of the genes encoding the L1 and L2 components of hemolysin BL from Bacillus cereus. Journal Bacteriology, v.179, n.8, p.2551-2556, 1997. - RAJKOWSKI, K. T. & BENNETT, R. W. (2003). Bacillus cereus. In M. D. Miliotis & J. W. Bier (Eds.) International Handbook of Foodborne Pathogens, (1? Edi??o). (688). CRC - RESENDE, J. R.; CARVALHO, V.D.; COSTA, L.; LEITE, I.P.; BARROS, E. B. XIII Congresso Brasileiro de Pesquisas Cafeeiras, Po?os de Caldas-MG, 1997. - REZENDE, N.C.M.; ROSSI JR., O.D.; AMARAL, L.A. Ocorr?ncia de bact?rias do grupo de Bacillus cereus em leite UHT integral (Ultra-High-Temperature). Rev. Bras. Cienc. Vet., v.7, p.162-166, 2000. - ROBINSON, R.K.; PHILL, M.A.D. Microbiologia lactol?gica. Zaragoza: Acribia, 1987, p. 18-32. - RUSUL, G.; YAACOB, N. H. Prevalence of Bacillus cereus in selected foods and detection of enterotoxin usin TECRA - VIA and BCET - RPLA. International Journal of Food Microbiology, v. 25, n. 2, p. 131-139, apr. 1995. - SALLUM Jr,B. Capitalismo e Cafeicultura: Oeste Paulista: 1888-1930. S?o Paulo, Duas Cidades, 1982. - SALVA, T.J.G.; LIMA, V.B. A composi??o qu?mica do caf? e as caracter?sticas da bebida e do gr?o. O agr?nomo, Campinas. v.59, n.1, p. 57-59, 2007. - SANTOS, D. F. S. Estrutura, conduta e desempenho do mercado exportador brasileiro de caf? em gr?o e de caf? sol?vel. Disserta??o (Mestrado). Vi?osa, MG: UFV, 1996. - SANTOS BS. 2000. Estudo de doze sorovares de Bacillus thuringiensis: Atividade larvicida, Curva de crescimento, Determina??o da Presen?a de Genes CRY 4A/B utilizando a PCR e Variabilidade Gen?mica. Observada por AP-PCR. MSC. Thesis, Instituto de Ci?ncias Biol?gicas, Universidade Federal de Minas Gerais ? UFMG, Belo Horizonte, Brasil, 131p. 67 - SCHOENI, J. L., & WONG, A. C. L. (2005). Heterogeneity observed in the components of hemolysin BL, an enterotoxin produced by Bacillus cereus. International Journal of Food Microbiology, 53, 159-167. - SILVA, C.F. Diversidade microbiana em gr?os de caf? (Coffea arabica L.) processados por via seca nas fases pr? e p?s-colheita. 120p. Tese (Mestrado). Universidade Federal de Lavras ? UFLA, Lavras, 2000. - SILVA, N, JUNQUEIRA, V.C. A, SILVEIRA, N.F.A. 1997. Manual de m?todos de An?lise Microbiol?gica de Alimentos. S?o Paulo: Livraria Varela, 295p. - SILVA JR, E. A. Manual de controle higi?nico-sanit?rio em alimentos. 2?ed. Livraria Varela, 1997, 385 p - SMITH RA & COUCHE GA (1991) The phylloplane as a source of Bacillus thuringiensis variants. Appl Environ Microbiol, 57: 311?315. - SCHNEPF, E.; CRICKMORE, N.; VAN RIE, J.; LERECLUS, D.; BAUM, J.; FEITELSON, J.; ZEIGLER, D. R.; DEAN, D. H. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiology and Molecular Biology Reviews, New York, v. 62, n. 3, p. 775-806, 1998. - SHINAGAWA, K. Analytical methods of Bacillus cereus and other Bacillus species, Int. J. Food Microbiol., v. 10, p. 125-141, 1990. - SHINAGAWA, K. Serology and characterisation of toxigenic Bacillus cereus. Netherlands Milk an Dairy Journal, v. 47, n. 2, p. 89-103, 1993. - SOARES, C. M. Bacillus cereus produtores de toxinas diarr?icas em servi?os de alimenta??o: an?lise da contamina??o ambiental e detec??o na linha de processamento de pratos c?rneos. Campinas, 2004. 150 p. Tese (Doutorado em Tecnologia de Alimentos) Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas. 2004. - SOUZA, C.M.O.C.C, ABRANTES, S.M.P & CAVADOS. C.F.G. Avalia??o dos Par?metros Microsc?picos do caf? torrado e mo?do interno e externo e Detec??o da 68 Presen?a de Bacillus cereus. Revista Brasileira de Vigil?ncia Sanit?ria. S?o Paulo. Vol. 1. n.1, p. 16-21. 2005. - SOUZA, S.M.C. DE & CARVALHO, V.L. de Efeito de microrganismos na qualidade da bebida de caf?. Informe Agropecu?rio, Belo Horizonte, 1997, vol. 18, n. 187, p. 21-26. - SOUZA, C.M.O.C.C. Avalia??o dos Par?metros Microsc?picos do caf? torrado e mo?do interno e externo e Detec??o da Presen?a de Bacillus cereus. Disserta??o de Mestrado, p.93. 2005. - SOUZA, C.M.O.C.C, ABRANTES, S.M.P & CAVADOS. C.F.G. Investiga??o f?sica qu?mica e microsc?pica de contaminantes em amostras de cafe torrado e mo?do comercializado no varejo. Revista Higiene Alimentar. , v.16, p.4 - 114, 2003. - STADHOUDERS. J. The enumeration of spores and vegetative cells of Bacillus cereus. Bull. Int. Dairy Fed., n.275, p.15-18, 1992. - VALADARES-INGLIS, M. C. C.; SOUZA, M. T.;SHILER, W.; Engenharia gen?tica de microrganismos agentes de controle biologico In: MELO, I. L.; AZEVEDO, J.L. Controle Biol?gico. Embrapa. Jaguari?na, p.102-225. 1998. - VALERO, M.; HERN?NDEZ-HERRERO, L. A.; FERN?NDEZ, P. S.; SALMER?N, M. C. Characterization of Bacillus cereus isolates from fresh vegetables and refrigerated minimally processed foods by biochemical and physiological tests. Food Microbiology, v. 19, n. 5, p. 491-499, oct. 2002. - VAUGHN, R.H.; CAMARGO, R.; FALLANGE, H.; MELLO AYRES, G. GEDEDELLO, A. Observations on teh microbiology of the coffee fermentation in Brazil. Food Technology, Chicago, v.12, p.12-57, 1958. - VARNAM.A.H, EVANS, M.G, Foodborne Pathogens-An illustrated Text. Ch 14. Wolfe Publishliing Ltd. London; p. 313-326,1991. 69 - VASCONCELOS FJM & RABINOVITCH L. A new formula for an alternative culture medium, without antibiotics, for isolation and presumptive quantification of Bacillus cereus in food. J. Food Microbiol. 1994; 58(3): 235-238. - VIDAL-MARTINS, A. M. C., ROSSI, O.D., REZENDE-LAGO, N. C. Microrganismos heterotr?ficos mes?filos e bact?rias do grupo do Bacillus cereus em leite integral submetido a ultra-alta temperatura. Arquivo Brasileiro de Medicina Veterin?ria e Zootecnia, Belo Horizonte, v. 57, n. 3, p. 396-400, 2005. - YAMAMOTO. T.; DEAN, D. H. Insecticidal proteins produced by bacteria pathogenic to agriculturas pests. In: CHARLES, J. F.; DELECLUSE. A.; NIELSEN-LE ROUX . C. (Ed). Emtomopathogenic bacteria: from laboratory to field application. Netherlands: kluwer Academic Publisher, 2000. p. 81-100. - WIJNANDS, L., DUFRENNE, J., & LEUSDEN, F. v. (2005). Bacillus cereus: characteristics, behaviour in the gastro-intestinal tract, and interaction with Caco-2 cells: National Institute for Public Health and Environment. - ZAMBOLIM, L.; RIBEIRO DO VALE, F.X. PEREIRA, A.A.; CHAVES, G.M. Caf? (Coffea arabica L.), controle de doen?as. In: Ribeiro do Vale, F.X.; Zambolim, L. Controle de doen?as de plantas: grandes culturas Vi?osa: Departamento de Fitopatologia; Bras?lia: Minist?rio da Agricultura e Abastecimento, v.2, p83-179, 1997. - ZAHNER V, MOMEN H, SALLES C A & RABINOVITCH L. 1989. A compartaive study of enzyme variation in Bacillus cereus and Bacillus thuringiensis. J. Appl Bacteriol. 67:275- 283. - ZAHNER V, 1992. An?lise isoenzim?tica de algumas esp?cies entomopatog?nicas de Bacillus. 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| dc.title.por.fl_str_mv |
Bacillus cereus: Isolamento, Contagem e Detec??o de enterotoxinas por PCR em amostras de caf? torrado e mo?do comercializado na cidade do Rio de Janeiro. |
| dc.title.alternative.eng.fl_str_mv |
Bacillus cereus: Isolation, Counting and Detection of enterotoxins by PCR in commercially roasted and ground coffee samples In Rio de Janeiro city. |
| title |
Bacillus cereus: Isolamento, Contagem e Detec??o de enterotoxinas por PCR em amostras de caf? torrado e mo?do comercializado na cidade do Rio de Janeiro. |
| spellingShingle |
Bacillus cereus: Isolamento, Contagem e Detec??o de enterotoxinas por PCR em amostras de caf? torrado e mo?do comercializado na cidade do Rio de Janeiro. Souza, Cyllene de Matos Ornelas da Cunha Corr?a de Bacillus cereus Caf? contamina??o Bacillus cereus contamination coffee Ci?ncia e Tecnologia de Alimentos |
| title_short |
Bacillus cereus: Isolamento, Contagem e Detec??o de enterotoxinas por PCR em amostras de caf? torrado e mo?do comercializado na cidade do Rio de Janeiro. |
| title_full |
Bacillus cereus: Isolamento, Contagem e Detec??o de enterotoxinas por PCR em amostras de caf? torrado e mo?do comercializado na cidade do Rio de Janeiro. |
| title_fullStr |
Bacillus cereus: Isolamento, Contagem e Detec??o de enterotoxinas por PCR em amostras de caf? torrado e mo?do comercializado na cidade do Rio de Janeiro. |
| title_full_unstemmed |
Bacillus cereus: Isolamento, Contagem e Detec??o de enterotoxinas por PCR em amostras de caf? torrado e mo?do comercializado na cidade do Rio de Janeiro. |
| title_sort |
Bacillus cereus: Isolamento, Contagem e Detec??o de enterotoxinas por PCR em amostras de caf? torrado e mo?do comercializado na cidade do Rio de Janeiro. |
| author |
Souza, Cyllene de Matos Ornelas da Cunha Corr?a de |
| author_facet |
Souza, Cyllene de Matos Ornelas da Cunha Corr?a de |
| author_role |
author |
| dc.contributor.advisor1.fl_str_mv |
Abrantes, Shirley de Mello Pereira |
| dc.contributor.advisor1ID.fl_str_mv |
512.535.507-00 |
| dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1296030772265453 |
| dc.contributor.referee1.fl_str_mv |
Amendoeira, Maria Regina Reis |
| dc.contributor.referee2.fl_str_mv |
Freitas, Sidinea Cordeiro de |
| dc.contributor.referee3.fl_str_mv |
Zamith, Helena Pereira da Silva |
| dc.contributor.referee4.fl_str_mv |
Luchese, Rosa Helena |
| dc.contributor.authorID.fl_str_mv |
517.467.525-72 |
| dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2287133994409786 |
| dc.contributor.author.fl_str_mv |
Souza, Cyllene de Matos Ornelas da Cunha Corr?a de |
| contributor_str_mv |
Abrantes, Shirley de Mello Pereira Amendoeira, Maria Regina Reis Freitas, Sidinea Cordeiro de Zamith, Helena Pereira da Silva Luchese, Rosa Helena |
| dc.subject.por.fl_str_mv |
Bacillus cereus Caf? contamina??o |
| topic |
Bacillus cereus Caf? contamina??o Bacillus cereus contamination coffee Ci?ncia e Tecnologia de Alimentos |
| dc.subject.eng.fl_str_mv |
Bacillus cereus contamination coffee |
| dc.subject.cnpq.fl_str_mv |
Ci?ncia e Tecnologia de Alimentos |
| description |
Coffee is one of the most appreciated drinks in the world. Coffee grounds are obtained from the fruit of a small plant that belongs to the genus Coffea. Coffea arabica and Coffea canephora robusta are the two species of highest commercial importance. They are more commonly known as arabica and robusta, respectively. Two-thirds of Coffea arabica plants are grown in South and Central America and Eastern Africa (the place of origin for this coffee species). Contamination with microorganisms has been a major factor affecting coffee quality, due to the method of harvest adopted in Brazil. Brazilian harvests are based on mixing fruits collected from the ground with those that fall on cloths. As the Bacillus cereus bacterium frequently uses soil as an environmental reservoir, it is easily capable of becoming a contaminant. In the current study, microbiological analysis and polymerase chain reaction (PCR) analysis of the toxicogenic profile of isolated strains were used to evaluate B. cereus contamination in commercial samples of ground and roasted coffee in the city of Rio de Janeiro. The results revealed a significant frequency of these enterotoxin genes in isolated strains, which were: The HBL (57%); HBL C (71%); HBL D (64%); The NHE (50%); NHE B (100%) ; NHE C (64%). Strains of B.thuringiensis were also found in 44% of the samples. |
| publishDate |
2011 |
| dc.date.issued.fl_str_mv |
2011-08-01 |
| dc.date.accessioned.fl_str_mv |
2020-08-21T00:56:40Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
| format |
doctoralThesis |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
SOUZA, Cyllene de Matos Ornelas da Cunha Corr?a de. Bacillus cereus: Isolamento, Contagem e Detec??o de enterotoxinas por PCR em amostras de caf? torrado e mo?do comercializado na cidade do Rio de Janeiro.. 2011. [91 f.]. Tese( Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos) - Universidade Federal Rural do Rio de Janeiro, Serop?dica. |
| dc.identifier.uri.fl_str_mv |
https://tede.ufrrj.br/jspui/handle/jspui/3836 |
| identifier_str_mv |
SOUZA, Cyllene de Matos Ornelas da Cunha Corr?a de. Bacillus cereus: Isolamento, Contagem e Detec??o de enterotoxinas por PCR em amostras de caf? torrado e mo?do comercializado na cidade do Rio de Janeiro.. 2011. [91 f.]. Tese( Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos) - Universidade Federal Rural do Rio de Janeiro, Serop?dica. |
| url |
https://tede.ufrrj.br/jspui/handle/jspui/3836 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.references.por.fl_str_mv |
- ABIC ? ASSOCIA??O BRASILEIRA DA IND?STRIA DE CAF?. Dispon?vel em: www.abic.com.br>. Acesso em 18 Jul. 2008 - AFONSO J?NIOR, P.C.; CORR?A, P.C.; GONELI, A.L.D.; SILVA, F.S. Contribui??o das etapas do pr?-processamento para a qualidade do caf?. Revista Brasileira de Armazenamento, Vi?osa, Especial Caf?, n. 8, p. 46-53, 2004. - ANG?LICO, C.L. Qualidade do caf? (Coffea ar?bica L.) em diferentes est?dios de matura??o e submetido a cinco tempos de ensacamentos antes da secagem. 2008.149f. Disserta??o (Mestrado em Ci?ncias dos Alimentos)- Universidade Federal de Lavras, lavras, MG. - ALVES, E.; CASTRO, H.A. Fungos associados ao caf? (Coffea arabica L.) nas fases de pr? e p?s-colheita em lavouras da regi?o de Lavras. Summa Phytopathologica, Jaboticabal, v. 24, n. 1, p. 4-7, 1998 - ALVES, S.B. Controle Microbiano de Insetos. 2aed. Piracicaba: FEALQ, 1998. - ANDERSSON, A.; R?NNER, U.; GRANUM, P. E. What problems does the food industry have the spore-forming pathogens Bacillus cereus and Clostridium perfringens? International Journal of Food Microbiology, v. 28, n. 2, p. 145-155, dec. 1995 - ANDERSSON, A., GRANUM, P. E., & RONNER, U. (1998). The adhesion of Bacillus cereus spores to epithelial cells might be an additional virulence mechanism. International Journal of Food Microbiology, 39, 93-99. - APHA (American Public Health Association). Microbiological monitoring of the food processing environment. In: Compendium of methods for the microbiological examination of foods. 3th. ed., Washington. Chap. 3, p.51: 75. 2001 - ARNESEN, L. P. S., FAGERLUND, A. & GRANUM, P. E. (2008). From soil to gut: Bacillus cereus and its food poisoning toxins. Acedido em 23-07-2008, http://www3.interscience.wiley.com/journal/120085025/abstract?CRETRY=1&SRETRY=0 54 - ATHIE, I.; PAULA, D.C. de. Insetos de gr?os armazenados ? aspectos biol?gicos e identifica??o. 2. ed. Sao Paulo: Varela. 2002. 244p. - AZEVEDO, J. L. Engenharia Gen?tica aplicada ao controle microbiano de insetos. In: ALVES, S.B. Controle Microbiano de Insetos. 2.ed. Piracicaba: FEALQ, 1998. - BACHHIL, V. N.; JAISWAL, T. N. Bacillus cereus in meats: incidence, prevalence and enterotoxigenicity. J. Food Sci. Technol., v. 25, n. 6, p. 371-372, Nov./ Dec. 1988 - BAPTIST J M, MANDEL,M & GHERMA R L.1978. Comparative zone eletrophoresis of enzymes in the Genus Bacillus. Int J Syst Bacteriol. 28:229-244. - BALBANI,A.P.S.; BUTUGAN.O. Contamina??o biol?gica de alimentos. REVIS?O E ENSAIO REVIEW AND ESSAY REVISI?N Y ENSAIO 2001. - BARRIOS, B.E.B. Caracteriza??o f?sica, qu?mica, microbiol?gica e sensorial de caf?s (Coffea arabica L.) da regi?o Alto Rio Grande ? Sul de Minas Gerais. 2001. 72 p.: il. Disserta??o (Mestrado em Ci?ncia dos Alimentos). Universidade Federal de Lavras, Lavras. - BARROS, V.R.M.; PANETTA, J.C., MIGUEL, O. Ocorr?ncia e n?veis de Bacillus cereus no leite em p? integral comercializado na capital do estado de S?o Paulo, Brasil ? 1987/1988. Rev. Educ. Contin. CRMV-SP, v.4, p.45-51, 2001. - BECKER, H.; SCHALLER, G; WIESE, W.; TERPLAN, G. Bacillus cereus in infant foods and dried milk products. International Journal of Food Microbiology, v. 23, n. 1, p. 1-15, set. 1994. - BEECHER, D.J.; MACMILLAN, J.D. Characterization of the components of hemolysin BL from Bacillus cereus. Infectionand Immunity, v.59,n.5, p.1778-1784, 1991. - BEECHER, D. J.; WONG, A. C. L. Identification and analysis of the antigens detected by two commercial Bacillus cereus diarrhoeal enterotoxin immunoassay kits. Applied and Environmental Microbiology, v. 60, n. 12, p. 4614-4616, dec. 1994. - BEECHER, D.J. Enterotoxic activity of hemolysin BL from Bacillus cereus. Infection and Immunity, v.63, n.11, p.4423-4428, 1995. 55 - BENEDICT, R. C.; PARTRIDGE, T.; WELLS, D.; BUCHANAN, R. L. Bacillus cereus: aerobic growth kinetics. Journal of Food Protection, v. 56, n. 3, p. 211-214, mar. 1993. - BERG, R. W.; SANDINE, W. E. Activation of bacterial spores. A review. Journal Milk Food Technology, v.33, p.435-441. 1970. - BENNETT, R. W.; BELAY, M. Bacillus cereus. In: DOWNES, F. P.; ITO, K. Compendium of methods for the microbiological examination of foods. 4th, American Public Health Association, cap. 32, p. 311-316, 2001. - BERNHARD, K.; JARRETT, P.; MEADOWS, M. et al. Natural isolates of Bacillus thuringiensis: worldwide distribution, characterization, and activity against insect pests. Journal of Invertebrate Pathology, Orlando, v.70, p.59-68, 1997. - BIOCHER, J. C. e BUSTA, F. F. Bacterial spore resistence to acid. Food Technology, v.37, p.87-89. 1983. - BHUNIA, A. K. (2007). Bacillus cereus and Bacillus anthracis. In A. K. Bhunia (Eds.) Foodborne Microbial Pathogens: Mechanisms and Pathogenesis;(1? Edi??o). (276). Springer. - BITANCOURT, A.A. O tratamento das cerejas de caf? para melhorar a bebida. O Biol?gico, S?o Paulo, 2001, vol. 23, n. 1, p. 1-11. - BOZZA, A.; TRALAMAZZA, S.M.; REYNAUD, D.T.; GABARDO, J.; VALASKI, J.C.; MARANGONI, P.B.; PIMENTEL, I.C. Isolamento de fungos associados a gr?os de caf? cv. Iapar 59 de origem de solo e ?rvore em diferentes tempos de colheita. Ci?ncia e Tecnologia de Alimentos, Campinas, v. 29, n. 3, p. 529-534, 2009. - BORGE, G. A.; SKEIE, M.; SORHAUG, T.; LANGSRUD, T.; GRANUM, P. E. Growth and toxin profiles of Bacillus cereus isolated from different food sources. International Journal of Food Microbiology, v. 69, n. 3, p. 237-246, sept. 2001. - BUGNO.A.;BUZZO.A.A.;NAKAMURA,C.T.;PEREIRA,T.C.;MATOS,D.;PINTO.T.J.A. Avalia??o da contamina??o microbiana em drogas vegetais. Revista Brasileira de 56 Ci?ncias Farmac?uticas Brazilian Journal of Pharmaceutical Sciences vol. 41, n. 4, out./dez., 2005. - BURDON K.L. 1946. J Bacterio. 52:665-678. - BURTON, G.R.W.; ENGELKIRK, P.G. Microbiologia para as ci?ncias da sa?de. Guanabara Koogan. Rio de Janeiro: 7? ed, 2005. - BRASIL, Resolu??o-RDC n? 12, de 2 de janeiro de 2001, da Ag?ncia Nacional de Vigil?ncia Sanit?ria, do Minist?rio da Sa?de - Aprova Regulamento T?cnico sobre padr?es microbiol?gicos para alimentos. Di?rio Oficial [da] Rep?blica Federativa do Brasil, Bras?lia, 10 de janeiro de 2001. - BRASIL, Resolu??o RDC n? 175 , de 08 de julho de 2003, da Ag?ncia Nacional de Vigil?ncia Sanit?ria, do Minist?rio da Sa?de - Aprova o regulamento t?cnico de Avalia??o de Mat?rias Macrosc?picas e Microsc?picas prejudiciais a Sa?de Humana em alimentos embalados. Di?rio Oficial [da] Rep?blica Federativa do Brasil, Bras?lia, 10 de julho de 2003. - BRASIL. Portaria n? 377, de 26 de abril de 1999. Institui normas b?sicas de alimentos. Di?rio Oficial da Uni?o [da] Rep?blica Federativa do Brasil, Bras?lia, Poder Executivo, Bras?lia, DF, 29 abr. 1999. - BRASIL. Portaria n ? 451, de 19 de setembro de 1997. Institui Princ?pios Gerais para o Estabelecimento de Crit?rios e Padr?es Microbiol?gicos para Alimentos. Di?rio Oficial da Uni?o [da] Rep?blica Federativa do Brasil, Bras?lia, Poder Executivo, Bras?lia, 19 de setembro de 1997. - BRAVO, A.; GILL, S. S.; SOBERON, M. Bacillus Thuringiensis: Mecanisms and use In:______. Comprehensive molecular inset science. Amsterdam:Elsevier BV, 2005. p.175- 206. - CARVALHO, V.D.; CHALFOUN, S.M. Aspectos qualitativos do caf?. Informe Agropecu?rio, Belo Horizonte, v. 11, n. 126, p. 79-92, 1985. 57 - CARVALHO,V.D; Chagas,S.J.R.;Souza,S.M.C. Fatores que afetam a qualidade do caf?. Informe Agropecu?rio, Belo Horizonte, 2005, v.18, n.187, p.5-20. - CARVALHO, V.D. 1997. Cafeicultura empresarial: produtividade e qualidade - qualidade do caf?. - COSTA, F.N, OLIVEIRA,E.M, BORGES,R.G. Ocorr?ncia de bact?rias da esp?cie Bacillus cereus em amostras de leite em p? integral obtidas no com?rcio varejista da cidade de S?o Lu?s, MA. Hig. aliment;18(121): 104-107, jun. 2004. - CHALFOUN, S.M.; CARVALHO, V.D. de. Microflora associada a frutos e gr?os de caf? de diferentes locais, tipos de colheita e diferentes etapas do preparo. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 15., 1989, Maring?, PR. Resumos... Rio de Janeiro: MIC/IBC, 1989, p. 17-21. - CHALFOUN, S.M.; PARIZZI, F.C. Fungos toxig?nicos e micotoxinas em caf?. In: BOR?M, F.M. P?s-colheita do caf?. Lavras: UFLA, 2008. cap. 14, p. 512-54. - CLAUS D & BERKELEY RCW. Genus Bacillus. In: Sneath PHA, Mair NS, Sharpe ME &Holt JG. Bergey?s Manual of Systematic Bacteriology. Baltimore: Williams & Wilkins; 1986, vol. 2, 1104-1139p. - CLAVEL, T., CARLIN, F., LAIRON, D., NGUYEN-THE, C., & SCHMITT, P. (2004). Survival of Bacillus cereus spores and vegetative cells in acid media simulating human stomach. Journal of Applied Microbiology, 97, 214-219. - CHRISTIANSSON, A. Enterotoxin production in milk by Bacillus cereus: a comparison of methods for toxin detection. Netherlands Milk and Dairy Journal, v. 47, n. 2, p. 79-87, 1993. - CHRISTIANSSON, A. The toxicology of Bacillus cereus. Bull. Int. Dairy Fed., n.275, p.30-35, 1992. 58 - CHILCOTT, C. N.; WIGLEY, P. J. Isolation and toxicity of Bacillus thuringiensis from soil and insect habitats in New Zealand. Journal of Invertebrate Pathology, v. 61, p. 244-247, 1993. - COPPING, L. G.; MENN, J. J. Review biopesticides: a review of their action, applications and efficacy. Pest management science, Sussex, v. 56, n. 8, p. 651-676, 2000. - COSENTINO, S.; MULARGIA, A.F.; PISANO, B. et al. Incidence and biochemical characteristics of Bacillus flora in Sardinian dairy products. Int. J. Food Microbiol., v.38, p.235-238, 1997. - DAMGAARD, P.H. Natural occurrence and dispersal of Bacillus thuringiensis in the environment. In: CHARLES, J.F.; DEL?CLUSE, A.; NIELSEN-LE ROUX, C. Entomopathogenic bacteria: from laboratory to field application. Netherlands: Kluwer Academic Publishers, 2000. p.23-40. - DE MAAGD, R.; BRAVO, A.; BERRY, C.; CRICKMORE, N.; SCHNEPF, H. E. Structure, diversity and evolution of protein toxins from spore-forming entomopathogenic bacteria. Annual Review Genetics, Palo Alto, v. 37, p. 409-433, 2003. - DELAZARi, I. Aspectos microbiol?gicos ligados a seguran?a e qualidade da carca?a de aves. In: Semana Acad?mica Veterin?ria, 8., 1998, S?o Paulo. Anais. S?o Paulo: 1998. p.71-77. - DOYLE, M. P. Bacillus cereus. Food Technology, Chicago, v.42, n.4, p.199-200, 1988 - DROBNIEWSKI, F. A. Bacillus cereus and related species. Clinical Microbiology Reviews, v. 6, n. 4, p. 324-338, oct. 1993. - EDWARDS, P.R, EWING, W.H. Identification of Enterobacteriaceae 3ed. Minneapolis: Burgess Publishing Co, 1972. - EHLING-SCHULZ, M., FRICKER, M. & SCHERER, S. (2004). Bacillus cereus, the causative agent of an emetic type of food-borne illness. 59 - EMBRAPA ? CAF?. Dispon?vel em:< http://www22.sede.embrapa.br/cafe/outros/relatorio_gestao.htm>.Acesso em 24 Jul.2008. - EDWARDS K.; JONHSTONE C.; THOMPSON C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucl. Acid Res., 19- 1349, 1991. - EVANS, J. A.; RUSSEL, S. L.; JAMES, C.; CORRY, J. E. L Microbial contamination of food refrigeration equipament. Journal of Food Engineering, v.62, n. 3, p. 225-232, may 2004. - ERLICH, H.A. PCR: technology: principles and applications for DNA amplification. New York/Oxford: Oxford University Press. 1992. - FERMANIAN, C.; LAPEYRE, C.; FR?MY, J. M.; CLAISSE, M. Production of diarrhoeal toxin by selected strains of Bacillus cereus. International Journal of Food Microbiology, v. 30, n. 3, p. 345-358, july 1996. - FERMANIAN, C. F.; WONG, A. C. L. Improved in vitro detection of hemolysin BL from Bacillus cereus. Int. J. Food Microbiol., v. 57, n. 2, p. 1-8, 2000. - FORSYTHE, S. J. Microbiologia da seguran?a alimentar. Porto Alegre: Artmed, 2002. - FRANCO, G. M. B.; Landgraf, M. Microbiologia dos Alimentos. S?o Paulo.: Editora Atheneu, 1996, cap.4, p. 33-81. - FRANCO, B. D. G. M.; LANDGRAF, M. Microrganismos patog?nicos de import?ncia em alimentos. In: FRANCO, B. D. G. M.; LANDGRAF, M. Microbiologia dos alimentos. S?o Paulo, Rio de Janeiro, Ribeir?o Preto, Belo Horizonte: Atheneu, 2006. cap. 4, p. 41-43. - FRITZE, D. (2004). Taxonomy of the Genus Bacillus and related genera: the aerobic endospore-forming bacteria. Acessada em 12/08/2009 http://apsjournals.apsnet.org/doi/pdf/10.1094/PHYTO.2004.94.11.1245?cookieSet=1. - GALLO, D; NAKANO, O.; SILVEIRA NETO, S.; BAPTISTA, G.C.; BERTI FILHO, E.; PARRA, J.R.P.; ZUCCHI, R.A.; ALVES, S.B.; VENDRAMIM, J.D.; MARCHINI, L.C.; LOPES, J.R.S.; OMOTO, S. Entomologia Agr?cola. Piracicaba: FEALQ, 2002. 920p 60 - GILBERT, R. J. Bacillus cereus gastroenteritis. In: RIEMANN, H.; BRYAN, L. F., eds. Foodborne Infections and intoxications. 2 ed. New York: Academic Press, 1979. p. 495- 518. - GUINEBRETIERE, M.H.; NGUYEN-THE, C. Sources of Bacillus cereus contamination in a pasteurised zucchini pur?e processing line, differentiated by two PCR-based methods. FEMS Microbiology Ecology, v.43, n.2, p.207-215, 2003. - GLARE, T.R.; O?CALLAGHAM,M. Bacillus thuringiensis: biology, ecology and safaty. Chichester: John Wiley, 2000. - GOEPFERT, J. M.; SPIRA, W. M.; KIM, H. U. Bacillus cereus: food poisoning organism. A review. Journal of Milk and Food Technology, v. 35, n. 4, p. 213-226, 1972. - GOMES.L.P.;RODRIGUES M.M.; SOARES.G.; BARONI.F.A.;SOUZA.M.M.S. Bacillus cereus em amostras de doces industrializados comercializados por ambulantes nos munic?pios de serop?dica e itagua? ? Rj. Rev. Univ. Rural, S?r. Ci. Vida. Serop?dica, RJ, EDUR, v. 24, n.2, Jul.-Dez., p.181-184, 2004. - GORDON R E, HAYNES W C & PANG C H N. 1973. The genus Bacillus. Agriculture Handbook n? 427. U.S. Department of Agriculture, Washington,D.C. - GORE, H.M.; SPIRA, W.M.; KIM, H.U. Real-time molecular beacon NASBA reveals HBLc expression from Bacillus spp. In milk . Biochem. Bioph. Res. Com., v. 311, p. 386- 390, 2003. - GRANUM, P.E. Bacillus cereus and its toxins. Journal of Applied Bacteriology, 1994, v.76, p.61S-66S. - GRANUM, P. E. Bacillus cereus. In: DOYLE, M. P., BEUCHAT, L.R. MONTVILLE, T.J. (Eds.), Food Microbiology. Fundamentals and Frontiers. Washington: ASM Press, 1997. p. 327?336. 61 - GRANUM, P. E, BAIRD-PARKER, T. Bacillus species. In: LUND, B. M.; BAIRDPARKER, T. C.; GOULD, G. W. The microbiological safety and quality of food. Gaithersburg, Maryland: Aspen Publishers, Inc. 2000. Vol. II. Chap. 39, p. 1029-1039. - GRANUM, P. E.; BRYNESTAD, S.; KRAMER, J.M. Analysis of enterotoxin production by Bacillus cereus from dairy products, food poisoning incidents and non-gastrointestinal infections. International Journal of Food Microbiology, v. 17, n. 4, p. 269-279, feb. 1993 - GRANUM, P. E.; LUND, T. Bacillus cereus and its food poisoning toxins. FEMS Microbiology Letters, v. 157, n. 2, p. 223-228, dec. 1997. - Granum, P. E., O? Sullivan, K., & Lund, T. (1999). The sequence of the non-haemolytic enterotoxin operon from Bacillus cereus. FEMS microbiology letters, 177, 225-229. - GRIFFITHS, M. W. Toxin production by psyhrotrophic Bacillus spp. present in milk. Journal of Food Protection, v. 53, n. 9, p. 790-792, sep. 1990. - GRIFFITHS, M. W. Bacillus cereus in liquid milk and other milk products. Bulletin of the International Dairy Federation, Brussells, n. 275, p.36-39, 1992. - HABIB, M.E.M.; ANDRADE, C.F.S. BACT?RIAS ENTOMOPATOG?NICAS. IN: Controle Microbiano de Insetos. Editado Por S.B. Alves. 2. Ed. Fealq, Piracicaba, 1998. p. 383-446. - HANSEN, B. M.; HENDRIKSEN, N. B. Detection of enterotoxin Bacillus cereus and Bacillus thuringiensis strains by PCR analysis. Applied and Environmental Microbiology, v. 67, n. 1, p. 185-189, jan. 2001. - HANSEN BM, DAMGAARD PH, EILENBERG J, & PEDERSEN JC (1998) Molecular and phenotypic characterization of Bacillus thuringiensis isolated from leaves and insects. J Invertebr Pathol, 71: 106?114. - HANSEN BM, DAMGAARD PH, EILENBERG J, & PEDERSEN JC (1996) Bacillus thuringiensis, ecology and environmental effects of its use for microbial pest control 62 (Environmental Project No. 316). Copenhagen, Denmark, Ministry of Environment and Energy, Danish Environmental Protection Agency. - HARRIS H & HOPKINSON D A. 1976. Handbook of enzyme electrophoresis in human genetics. Elsevier Science Publish, New York. - HARRIS, H. & HOPKINSON, D.A. Handbook of enzyme electrophoresis in human genetics. Amsterdam: North- Holland, 1976. - HARWOOD. CR & CUTTING S.M. Molecular Biological Methods for Bacillus. Wiley e Sons Ltda, West Sussex, England; 1990. 65p. - HEALTH PROTECTION BRANCH. Guidelines for the general cleanliness of food: an Overview (Hpb). Ottawa, Ontario, 1999. 9p. - HEINRICHS, A.H. et al. Molecular cloning and characterization of the HBLA gene encoding the B component of hemolysin BL from Bacillus cereus. Journal Bacteriology, v.175, p.6760- 6766, 1993. - HOFTE, H.; WHITELEY, H.R. Inseticidal Crystal Proteins of Bacillus thuringiensis. Microbiological Review, Washington, v. 53, n. 2, p. 242-255, 1989. - HUSMARK, U.; SIK, U. R. Forces involved in adhesion of Bacillus cereus spores to solid surfaces under different environmental conditions. The Journal of Applied Bacteriology, v. 69, n. 4, p. 557-562, oct. 1990. - ILLY, A.; VIANI, R. Espresso coffee: the chemistry of quality. San Diego: Academic Press, 1995. 253 p. - JOHNSON, K. M. Bacillus cereus foodborne illness - an update. Journal of Food Protection, v. 47, n. 2, p. 145-153, feb. 1983. - JOHNSON, K. M.; NELSON, C. L.; BUSTA, F. F. Influence of temperature on germination and growth of spores of emetic and diarrheal strains of Bacillus cereus in a broth medium and in rice. Journal of Food Science, Chicago, v. 48, n. 1, p. 286-287, 1983 63 - J??SKEL?INEN, E. (2008). Assessment and control of Bacillus cereus emetic toxin in food. Department of Applied Chemistry and Microbiology - Division Microbiology. Helsinki, University of Helsinki: 74. - JAY, J. M. (2005). Bacillus cereus gastroenteritis. In J. M. Jay (Eds.) Modern Food Microbiology, (6? Edi??o). (720). Springer. - KNIEHL, E., BECKER, A. AND FORSTER, D. H. (2003). Pseudo-outbreak of toxigenic Bacillus cereus isolated from stools of three patients with diarrhoea after oral administration of a probiotic medication. Journal of Hospital Infection 55, 33-38. - KOTIRANTA, A.; LOUNATMAA, K., HAAPASALO, M. Epidemiology and pathogenesis of Bacillus cereus infections. Microbes and Infection, v. 2, n.2, p. 189-198, feb. 2000. - KUSKE, C. R., BANTON, K. L., ADORADA, D. L., STARK, P. C., HILL, K. K. & JACKSON, P. J. (1998). Small-Scale DNA Sample Preparation Method for Field PCR Detection of Microbial Cells and Spores in Soil. Appl. Environ. Microbiol., 64(7), 2463- 2472. - LAGO.N.C.M.R.;ROSSI.O.D.;VIDAL MARTINS.; AMARAL.L.A. Ocorr?ncia de Bacillus cereus em leite integral e capacidade enterotoxig?nica das cepas isoladas. Arq. Bras. Med. Vet. Zootec., v.59, n.6, p.1563-1569, 2007. - LI, CARREL, J. J.; ELLAR, D. J. Cristal estructure of insecticide delta-endotoxin from Bacillus thuringiensis at 2,5 A resolution. Nature, London, v. 353, n. 6.347, p.815-821, 1991. - LINDBACK, T., FAGERLUND, A., RODLANDT, M. S., & GRANUM, P. E. (2004). Characterization of the Bacillus cereus NHE enterotoxin. Microbiology, 150, 3959-3967 - LUND, T.; GRANUM, P.E. Characterisation of a nonhaemolytic enterotoxin complex from Bacillus cereus isolated after a food borne outbreak. FEMS Microbiology Letters, v.141, n.2-3, p.151-156, 1996. 64 - LUTHY P., WOLFERSBERGER, M.G. Pathogenisis of Bacillus thuringiensis toxin. In CHARLES J.F., DELECLUSE A., NIELSEN-LE ROUX, C. (Eds.) Entomopathogenic bacteria: from laboratory to field application. Kluwer Academic Publishers, 2000. p.167-180. - MALORNY, B. et al. Standardization of diagnostic PCR for detection of foodborne pathogens. Int. J. Food Microbiol., Amsterdam, v. 83, n. 1, p. 39-48, 2003. - M?NTYNEN, V.; LINDSTR?M, K. A Rapid PCR-Based DNA Test for Enterotoxic Bacillus cereus. Applied and Environmental Microbiology, Washington, v.64, n.5, p. 1634-1639, May 1998. - MARTINS. H. M, MARTINS. M. L, BERNADO, F. Caracteriza??o microbiol?gica e detec??o de aflatoxina e de ocratoxina A em gr?os de caf? torrados, comercializados em Portugal. 2004, Revista de Ci?ncias Agr?rias. Lisboa, Portugal. - MARTIN, P.A.; TRAVERS, R.S. Worldwide abundance and distribution of Bacillus thuringiensis isolates. Applied and Environmental Microbiology, Massachusetts, v.55, n.10, p.2437-2442, 1989. - MEADOWS MP, ELLIS DJ, BUTT J, JARRETT P, & BURGES HD (1992) Distribution, frequency and diversity of Bacillus thuringiensis in an animal feed mill. Appl Environ Microbiol, 58(4): 1344?1350. - MINNAARD, J.; HUMEN, M.; P?REZ, P. F. Bacillus cereus exocellular factors on human intestinal epithelial cells. Journal of Food Protection, v. 64, n. 10, p. 1535-1541, oct. 2001 - MONNERAT, R; BRAVO A. Prote?nas bioinseticidas produzidas pela bact?ria Bacillus thuringiensis: modo de a??o e resist?ncia. In: MELO IS, AZEVEDO JL, (eds). Controle Biol?gico. Jaguari?na: Embrapa Meio Ambiente. 2000. p.163. - NOTERMANS, S.; BATT, C. A. A risk assessment approach for food-borne Bacillus cereus and its toxins. Journal of Applied Microbiology, Readin, v. 84, p. 51S-61S, 1998 (Supplement Symposium). 65 - ODOMERU, J. A.; TONER, A. K.; MUCKLE, C. A.; GRIFFITHS, M. W.; LYNCH, J. A. Detection of Bacillus cereus diarrheal enterotoxin in raw and pasteurized milk. Journal of Food Protection, Des Moines, v. 30, n.11, p.1391-1393, 1997. - PELCZAR J.M; CHAN S.C.E; KRIEG R.N. Microbiologia: conceitos e aplica??es. 2.ed. S?o Paulo: Editora Afiliada, 1996. v. 2, p. 306-311. - PEREIRA, M. S.; OLIVEIRA, L. A. T.; FRANCO, R. M. C.; PRADO, J. C. A. Contagem, isolamento e identifica??o de Bacillus cereus em condimentos preparados, utilizados em embutido c?rneo (mortadela). Revista Brasileira de Ci?ncia Veterin?ria, v. 6, n. 3, p. 137- 140, set.-dez. 1999. - PIELAAT, A., WIJNANDS, L. M., TAKUMI, K., NAUTA, M. J., & VAN LEUSDEN, F. M. (2006). The fate of Bacillus cereus in gastro-intestinal tract: National Institute for Public Health and Environment. - PRIEST, F. G. Systematics and ecology of Bacillus. In: SONESHEIN, A. L., HOCH, J. A.; LOSIK, R. (ed.). Bacillus subtilis and other Gram-positive bacteria. Washington, DC: American Society for Microbiology, 1993. p. 3-16. - PR?SS, B. M.; DIETRICH, R.; NIBLER, B.; M?RTLBAUER, E.; SCHERER, S. The hemolytic enterotoxin HBL is broadly distributed among species of the Bacillus cereus group. Applied and Environmental Microbiology, v. 65, n. 12, p. 5436-5442, dec. 1999. - RABINOVITCH, L; VICENTE MMA; GUAYCUR?S TV; FREITAS JPGV & MESQUITA RP.. Avalia??o da incid?ncia e da toxicidade de amostras de Bacillus cereus em diferentes classes de alimentos comercializados e consumidos no Estado do Rio de Janeiro.Mem Inst Oswaldo Cruz , 1985, 80(1):1-9. - RADHIKA, B.; PADMAPRIYA, B. P.; CHANDRASHEKAR, A.; KESHAVA, N. and VARADAJ, M. C. Detection of Bacillus cereus in foods by colony hibridization using PCR-generated probe and characterization of isolates for toxins by PCR. International Journal of Food Microbiology, V. 74, p. 131-138, 2002. 66 - RYAN, P.A. et al. Molecular cloning and characterization of the genes encoding the L1 and L2 components of hemolysin BL from Bacillus cereus. Journal Bacteriology, v.179, n.8, p.2551-2556, 1997. - RAJKOWSKI, K. T. & BENNETT, R. W. (2003). Bacillus cereus. In M. D. Miliotis & J. W. Bier (Eds.) International Handbook of Foodborne Pathogens, (1? Edi??o). (688). CRC - RESENDE, J. R.; CARVALHO, V.D.; COSTA, L.; LEITE, I.P.; BARROS, E. B. XIII Congresso Brasileiro de Pesquisas Cafeeiras, Po?os de Caldas-MG, 1997. - REZENDE, N.C.M.; ROSSI JR., O.D.; AMARAL, L.A. Ocorr?ncia de bact?rias do grupo de Bacillus cereus em leite UHT integral (Ultra-High-Temperature). Rev. Bras. Cienc. Vet., v.7, p.162-166, 2000. - ROBINSON, R.K.; PHILL, M.A.D. Microbiologia lactol?gica. Zaragoza: Acribia, 1987, p. 18-32. - RUSUL, G.; YAACOB, N. H. Prevalence of Bacillus cereus in selected foods and detection of enterotoxin usin TECRA - VIA and BCET - RPLA. International Journal of Food Microbiology, v. 25, n. 2, p. 131-139, apr. 1995. - SALLUM Jr,B. Capitalismo e Cafeicultura: Oeste Paulista: 1888-1930. S?o Paulo, Duas Cidades, 1982. - SALVA, T.J.G.; LIMA, V.B. A composi??o qu?mica do caf? e as caracter?sticas da bebida e do gr?o. O agr?nomo, Campinas. v.59, n.1, p. 57-59, 2007. - SANTOS, D. F. S. Estrutura, conduta e desempenho do mercado exportador brasileiro de caf? em gr?o e de caf? sol?vel. Disserta??o (Mestrado). Vi?osa, MG: UFV, 1996. - SANTOS BS. 2000. Estudo de doze sorovares de Bacillus thuringiensis: Atividade larvicida, Curva de crescimento, Determina??o da Presen?a de Genes CRY 4A/B utilizando a PCR e Variabilidade Gen?mica. Observada por AP-PCR. MSC. Thesis, Instituto de Ci?ncias Biol?gicas, Universidade Federal de Minas Gerais ? UFMG, Belo Horizonte, Brasil, 131p. 67 - SCHOENI, J. L., & WONG, A. C. L. (2005). Heterogeneity observed in the components of hemolysin BL, an enterotoxin produced by Bacillus cereus. International Journal of Food Microbiology, 53, 159-167. - SILVA, C.F. Diversidade microbiana em gr?os de caf? (Coffea arabica L.) processados por via seca nas fases pr? e p?s-colheita. 120p. Tese (Mestrado). Universidade Federal de Lavras ? UFLA, Lavras, 2000. - SILVA, N, JUNQUEIRA, V.C. A, SILVEIRA, N.F.A. 1997. Manual de m?todos de An?lise Microbiol?gica de Alimentos. S?o Paulo: Livraria Varela, 295p. - SILVA JR, E. A. Manual de controle higi?nico-sanit?rio em alimentos. 2?ed. Livraria Varela, 1997, 385 p - SMITH RA & COUCHE GA (1991) The phylloplane as a source of Bacillus thuringiensis variants. Appl Environ Microbiol, 57: 311?315. - SCHNEPF, E.; CRICKMORE, N.; VAN RIE, J.; LERECLUS, D.; BAUM, J.; FEITELSON, J.; ZEIGLER, D. R.; DEAN, D. H. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiology and Molecular Biology Reviews, New York, v. 62, n. 3, p. 775-806, 1998. - SHINAGAWA, K. Analytical methods of Bacillus cereus and other Bacillus species, Int. J. Food Microbiol., v. 10, p. 125-141, 1990. - SHINAGAWA, K. Serology and characterisation of toxigenic Bacillus cereus. Netherlands Milk an Dairy Journal, v. 47, n. 2, p. 89-103, 1993. - SOARES, C. M. Bacillus cereus produtores de toxinas diarr?icas em servi?os de alimenta??o: an?lise da contamina??o ambiental e detec??o na linha de processamento de pratos c?rneos. Campinas, 2004. 150 p. Tese (Doutorado em Tecnologia de Alimentos) Faculdade de Engenharia de Alimentos, Universidade Estadual de Campinas. 2004. - SOUZA, C.M.O.C.C, ABRANTES, S.M.P & CAVADOS. C.F.G. Avalia??o dos Par?metros Microsc?picos do caf? torrado e mo?do interno e externo e Detec??o da 68 Presen?a de Bacillus cereus. Revista Brasileira de Vigil?ncia Sanit?ria. S?o Paulo. Vol. 1. n.1, p. 16-21. 2005. - SOUZA, S.M.C. DE & CARVALHO, V.L. de Efeito de microrganismos na qualidade da bebida de caf?. Informe Agropecu?rio, Belo Horizonte, 1997, vol. 18, n. 187, p. 21-26. - SOUZA, C.M.O.C.C. Avalia??o dos Par?metros Microsc?picos do caf? torrado e mo?do interno e externo e Detec??o da Presen?a de Bacillus cereus. Disserta??o de Mestrado, p.93. 2005. - SOUZA, C.M.O.C.C, ABRANTES, S.M.P & CAVADOS. C.F.G. Investiga??o f?sica qu?mica e microsc?pica de contaminantes em amostras de cafe torrado e mo?do comercializado no varejo. Revista Higiene Alimentar. , v.16, p.4 - 114, 2003. - STADHOUDERS. J. The enumeration of spores and vegetative cells of Bacillus cereus. Bull. Int. Dairy Fed., n.275, p.15-18, 1992. - VALADARES-INGLIS, M. C. C.; SOUZA, M. T.;SHILER, W.; Engenharia gen?tica de microrganismos agentes de controle biologico In: MELO, I. L.; AZEVEDO, J.L. Controle Biol?gico. Embrapa. Jaguari?na, p.102-225. 1998. - VALERO, M.; HERN?NDEZ-HERRERO, L. A.; FERN?NDEZ, P. S.; SALMER?N, M. C. Characterization of Bacillus cereus isolates from fresh vegetables and refrigerated minimally processed foods by biochemical and physiological tests. Food Microbiology, v. 19, n. 5, p. 491-499, oct. 2002. - VAUGHN, R.H.; CAMARGO, R.; FALLANGE, H.; MELLO AYRES, G. GEDEDELLO, A. Observations on teh microbiology of the coffee fermentation in Brazil. Food Technology, Chicago, v.12, p.12-57, 1958. - VARNAM.A.H, EVANS, M.G, Foodborne Pathogens-An illustrated Text. Ch 14. Wolfe Publishliing Ltd. London; p. 313-326,1991. 69 - VASCONCELOS FJM & RABINOVITCH L. A new formula for an alternative culture medium, without antibiotics, for isolation and presumptive quantification of Bacillus cereus in food. J. Food Microbiol. 1994; 58(3): 235-238. - VIDAL-MARTINS, A. M. C., ROSSI, O.D., REZENDE-LAGO, N. C. Microrganismos heterotr?ficos mes?filos e bact?rias do grupo do Bacillus cereus em leite integral submetido a ultra-alta temperatura. Arquivo Brasileiro de Medicina Veterin?ria e Zootecnia, Belo Horizonte, v. 57, n. 3, p. 396-400, 2005. - YAMAMOTO. T.; DEAN, D. H. Insecticidal proteins produced by bacteria pathogenic to agriculturas pests. In: CHARLES, J. F.; DELECLUSE. A.; NIELSEN-LE ROUX . C. (Ed). Emtomopathogenic bacteria: from laboratory to field application. Netherlands: kluwer Academic Publisher, 2000. p. 81-100. - WIJNANDS, L., DUFRENNE, J., & LEUSDEN, F. v. (2005). Bacillus cereus: characteristics, behaviour in the gastro-intestinal tract, and interaction with Caco-2 cells: National Institute for Public Health and Environment. - ZAMBOLIM, L.; RIBEIRO DO VALE, F.X. PEREIRA, A.A.; CHAVES, G.M. Caf? (Coffea arabica L.), controle de doen?as. In: Ribeiro do Vale, F.X.; Zambolim, L. Controle de doen?as de plantas: grandes culturas Vi?osa: Departamento de Fitopatologia; Bras?lia: Minist?rio da Agricultura e Abastecimento, v.2, p83-179, 1997. - ZAHNER V, MOMEN H, SALLES C A & RABINOVITCH L. 1989. A compartaive study of enzyme variation in Bacillus cereus and Bacillus thuringiensis. J. Appl Bacteriol. 67:275- 283. - ZAHNER V, 1992. An?lise isoenzim?tica de algumas esp?cies entomopatog?nicas de Bacillus. 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