Efeito do processamento t?rmico nas caracter?sticas f?sicoqu?micas, nutricionais, microbiol?gicas e na atividade enzim?tica de polpa de mam?o Formosa (Carica papaya L.)

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Monique Lopes
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFRRJ
Texto Completo: https://tede.ufrrj.br/jspui/handle/jspui/2376
Resumo: Brazil is responsible for 25 % of total papaya production in the world, a fruit wich is mainly characterized for its nutritional aspects. The juice and pulp industries have showed an increase in their production in the last years. However, this segment takes only 5% of total papaya produced, while the remaining is consumed as a fresh fruit. This scene is unfavorable, because the high rate of losses during times of big harvests. The processing of papaya can minimize the losses, increasing its shelf life and adding value to this tropical fruit. One of the most used conservation method is HTST pasteurization (high temperature, short time), wich reduces microbial count to safe levels for the consumers and inactivates pectinesterase. This enzyme has an important role during fruit ripening, but it becomes undesirable after process, because acts in pectin and causes undesirable changes in the pulp. The main objective of this study was investigate the effects of temperature and process time on the pectinesterase activity in papaya Formosa pulp. The effects of thermal process on the physical and chemical, nutritional, microbiological and sensorial characteristics were also evaluated. The experiments were carried out following a 22 factorial design with central point. Two independents variables were studied, temperature and time, in the range of 75 to 95?C / 20 to 60 s. The results were submitted to analyses of variance with 95% of confidence interval. In the range of study, factors temperature, process time and interaction did not show significant effects in the characteristics such as pH, titratable acidity, soluble solids contents, vitamin B1, B2 and C contents, antioxidant activity, phenolic compounds, carotenoids contents and colour parameters (L, a, b e ?E). Temperature showed a positive significant (p<0,05) linear effect in the reduction of pectinesterase activity. The best time and temperature ranges for heat processing, which reached more than 95 % of enzyme inactivation were between 20 and 60 seconds at 95?C. The process at 95 ? C/ 60 s promoted a significant decrease in vitamin C content, compared to fresh pulp (Dunnett test at 5% probability level), while the other nutrients did not show significant losses. The presence of coliforms at 45 ?C and Salmonella was not detected. The applied treatments were sufficient to promote a reduction of about 3 logarithmic cycles in the population of molds and yeasts. The Preference Map obtained by the acceptance analyses showed a trend of preference for the pasteurized at 95 ?C / 60 s, 95 ?C / 20 s and fresh samples, while commercial pulps were rejected by consumers.
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spelling Furtado, Angela Aparecida Lemos878.390.327-53Furtado, Angela Aparecida LemosCabral, Lurdes Maria Corr?aFreitas, Suely PereiraGodoy, Ronoel Luiz de OliveiraBorguini, Renata Galhardo083.509.317-44http://lattes.cnpq.br/0084923069422892Ribeiro, Monique Lopes2018-08-23T21:04:05Z2009-03-27RIBEIRO, Monique Lopes. Efeito do processamento t?rmico nas caracter?sticas f?sico-qu?micas, nutricionais, microbiol?gicas e na atividade enzim?tica de polpa de mam?o Formosa (Carica papaya L.). 2009. 103 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos). Instituto de Tecnologia, Departamento de Tecnologia de Alimentos, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2009.https://tede.ufrrj.br/jspui/handle/jspui/2376Brazil is responsible for 25 % of total papaya production in the world, a fruit wich is mainly characterized for its nutritional aspects. The juice and pulp industries have showed an increase in their production in the last years. However, this segment takes only 5% of total papaya produced, while the remaining is consumed as a fresh fruit. This scene is unfavorable, because the high rate of losses during times of big harvests. The processing of papaya can minimize the losses, increasing its shelf life and adding value to this tropical fruit. One of the most used conservation method is HTST pasteurization (high temperature, short time), wich reduces microbial count to safe levels for the consumers and inactivates pectinesterase. This enzyme has an important role during fruit ripening, but it becomes undesirable after process, because acts in pectin and causes undesirable changes in the pulp. The main objective of this study was investigate the effects of temperature and process time on the pectinesterase activity in papaya Formosa pulp. The effects of thermal process on the physical and chemical, nutritional, microbiological and sensorial characteristics were also evaluated. The experiments were carried out following a 22 factorial design with central point. Two independents variables were studied, temperature and time, in the range of 75 to 95?C / 20 to 60 s. The results were submitted to analyses of variance with 95% of confidence interval. In the range of study, factors temperature, process time and interaction did not show significant effects in the characteristics such as pH, titratable acidity, soluble solids contents, vitamin B1, B2 and C contents, antioxidant activity, phenolic compounds, carotenoids contents and colour parameters (L, a, b e ?E). Temperature showed a positive significant (p<0,05) linear effect in the reduction of pectinesterase activity. The best time and temperature ranges for heat processing, which reached more than 95 % of enzyme inactivation were between 20 and 60 seconds at 95?C. The process at 95 ? C/ 60 s promoted a significant decrease in vitamin C content, compared to fresh pulp (Dunnett test at 5% probability level), while the other nutrients did not show significant losses. The presence of coliforms at 45 ?C and Salmonella was not detected. The applied treatments were sufficient to promote a reduction of about 3 logarithmic cycles in the population of molds and yeasts. The Preference Map obtained by the acceptance analyses showed a trend of preference for the pasteurized at 95 ?C / 60 s, 95 ?C / 20 s and fresh samples, while commercial pulps were rejected by consumers.O Brasil ? respons?vel por 25% do total da produ??o mundial de mam?o, um fruto que se caracteriza principalmente por seus aspectos nutricionais. A ind?stria de sucos e polpas tem apresentado um aumento na sua produ??o nos ?ltimos anos. Entretanto, este segmento aproveita somente 5 % do total de mam?o produzido, sendo o restante consumido in natura. Este quadro ? desfavor?vel tendo em vista os altos ?ndices de perdas nas ?pocas de grandes safras. O processamento em forma de polpa minimiza as perdas, pois aumenta a vida ?til e agrega valor econ?mico ao fruto. Um dos tratamentos mais aplicados para a polpa de mam?o ? a pasteuriza??o do tipo HTST (High Temperature, Short Time), cuja finalidade ? reduzir a carga microbiana a n?veis seguros para os consumidores e inativar a enzima pectinesterase. Esta enzima, que tem um papel fundamental na matura??o do fruto, se torna indesej?vel ap?s o processamento, pois atua sobre a pectina causando altera??es indesej?veis na polpa. Este trabalho teve como principal objetivo avaliar o efeito da temperatura e tempo de processo na atividade da enzima pectinesterase presente na polpa de mam?o Formosa. Tamb?m foram avaliados os efeitos do tratamento t?rmico nas caracter?sticas microbiol?gicas, f?sico-qu?micas, nutricionais e sensoriais da polpa. Os experimentos foram realizados seguindo um planejamento fatorial completo 22 com ponto central, sendo as vari?veis independentes o tempo e a temperatura de processo, em uma faixa de observa??o entre 75 ?C e 90 ?C / 20 s e 60 s. Os dados foram submetidos ? an?lise de vari?ncia com intervalo de confian?a de 95 %. Na faixa observada, tanto os fatores tempo e temperatura como a intera??o entre os fatores n?o apresentaram efeitos significativos nas caracter?sticas como pH, acidez titul?vel, teor de vitamina B1, teor de vitamina B2, teor de vitamina C, teor de caroten?ides, atividade antioxidante, teor de compostos fen?licos e par?metros da cor (L, a, b e ?E). O fator temperatura apresentou um efeito linear significativo (p < 0,05) e positivo para redu??o da atividade da pectinesterase, sendo que os tratamentos a 95 ?C / 20 s e 95 ?C / 60 s foram os que apresentaram maior percentual de redu??o, com aproximadamente 95 %. Em compara??o ? polpa in natura, somente a polpa submetida a 95 ?C / 60 s apresentou um decr?scimo significativo no teor de vitamina C (pelo teste de Dunnett ao n?vel de 5 %), sendo que para os outros nutrientes n?o houve decr?scimo significativo. Nas an?lises microbiol?gicas n?o foi detectada a presen?a de coliformes a 45 ?C e de Salmonella sp. Os tratamentos aplicados promoveram uma redu??o na ordem de 103 na contagem total de fungos e leveduras. Na an?lise sensorial, o Mapa Interno de Prefer?ncia obtido a partir dos dados de aceita??o mostraram uma tend?ncia ? prefer?ncia pelas amostras pasteurizadas a 95 ?C / 60 s, 95 ?C / 20 s e in natura, enquanto que as polpas de marcas comerciais foram rejeitadas.Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-08-23T21:04:05Z No. of bitstreams: 1 2009 - Monique Lopes Ribeiro.pdf: 2169069 bytes, checksum: d331284a6463585cb4755e8e93b90be8 (MD5)Made available in DSpace on 2018-08-23T21:04:05Z (GMT). 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dc.title.por.fl_str_mv Efeito do processamento t?rmico nas caracter?sticas f?sicoqu?micas, nutricionais, microbiol?gicas e na atividade enzim?tica de polpa de mam?o Formosa (Carica papaya L.)
dc.title.alternative.eng.fl_str_mv Effect of thermal process on the physical and chemical, nutritional, microbiological characteristics and enzymatic activity in papaya pulp
title Efeito do processamento t?rmico nas caracter?sticas f?sicoqu?micas, nutricionais, microbiol?gicas e na atividade enzim?tica de polpa de mam?o Formosa (Carica papaya L.)
spellingShingle Efeito do processamento t?rmico nas caracter?sticas f?sicoqu?micas, nutricionais, microbiol?gicas e na atividade enzim?tica de polpa de mam?o Formosa (Carica papaya L.)
Ribeiro, Monique Lopes
Pasteuriza??o
Pectinesterase
Carica papaya L.
Pasteurization
Pectinesterase
Ci?ncia e Tecnologia de Alimentos
title_short Efeito do processamento t?rmico nas caracter?sticas f?sicoqu?micas, nutricionais, microbiol?gicas e na atividade enzim?tica de polpa de mam?o Formosa (Carica papaya L.)
title_full Efeito do processamento t?rmico nas caracter?sticas f?sicoqu?micas, nutricionais, microbiol?gicas e na atividade enzim?tica de polpa de mam?o Formosa (Carica papaya L.)
title_fullStr Efeito do processamento t?rmico nas caracter?sticas f?sicoqu?micas, nutricionais, microbiol?gicas e na atividade enzim?tica de polpa de mam?o Formosa (Carica papaya L.)
title_full_unstemmed Efeito do processamento t?rmico nas caracter?sticas f?sicoqu?micas, nutricionais, microbiol?gicas e na atividade enzim?tica de polpa de mam?o Formosa (Carica papaya L.)
title_sort Efeito do processamento t?rmico nas caracter?sticas f?sicoqu?micas, nutricionais, microbiol?gicas e na atividade enzim?tica de polpa de mam?o Formosa (Carica papaya L.)
author Ribeiro, Monique Lopes
author_facet Ribeiro, Monique Lopes
author_role author
dc.contributor.advisor1.fl_str_mv Furtado, Angela Aparecida Lemos
dc.contributor.advisor1ID.fl_str_mv 878.390.327-53
dc.contributor.referee1.fl_str_mv Furtado, Angela Aparecida Lemos
dc.contributor.referee2.fl_str_mv Cabral, Lurdes Maria Corr?a
dc.contributor.referee3.fl_str_mv Freitas, Suely Pereira
dc.contributor.referee4.fl_str_mv Godoy, Ronoel Luiz de Oliveira
dc.contributor.referee5.fl_str_mv Borguini, Renata Galhardo
dc.contributor.authorID.fl_str_mv 083.509.317-44
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/0084923069422892
dc.contributor.author.fl_str_mv Ribeiro, Monique Lopes
contributor_str_mv Furtado, Angela Aparecida Lemos
Furtado, Angela Aparecida Lemos
Cabral, Lurdes Maria Corr?a
Freitas, Suely Pereira
Godoy, Ronoel Luiz de Oliveira
Borguini, Renata Galhardo
dc.subject.por.fl_str_mv Pasteuriza??o
Pectinesterase
Carica papaya L.
topic Pasteuriza??o
Pectinesterase
Carica papaya L.
Pasteurization
Pectinesterase
Ci?ncia e Tecnologia de Alimentos
dc.subject.eng.fl_str_mv Pasteurization
Pectinesterase
dc.subject.cnpq.fl_str_mv Ci?ncia e Tecnologia de Alimentos
description Brazil is responsible for 25 % of total papaya production in the world, a fruit wich is mainly characterized for its nutritional aspects. The juice and pulp industries have showed an increase in their production in the last years. However, this segment takes only 5% of total papaya produced, while the remaining is consumed as a fresh fruit. This scene is unfavorable, because the high rate of losses during times of big harvests. The processing of papaya can minimize the losses, increasing its shelf life and adding value to this tropical fruit. One of the most used conservation method is HTST pasteurization (high temperature, short time), wich reduces microbial count to safe levels for the consumers and inactivates pectinesterase. This enzyme has an important role during fruit ripening, but it becomes undesirable after process, because acts in pectin and causes undesirable changes in the pulp. The main objective of this study was investigate the effects of temperature and process time on the pectinesterase activity in papaya Formosa pulp. The effects of thermal process on the physical and chemical, nutritional, microbiological and sensorial characteristics were also evaluated. The experiments were carried out following a 22 factorial design with central point. Two independents variables were studied, temperature and time, in the range of 75 to 95?C / 20 to 60 s. The results were submitted to analyses of variance with 95% of confidence interval. In the range of study, factors temperature, process time and interaction did not show significant effects in the characteristics such as pH, titratable acidity, soluble solids contents, vitamin B1, B2 and C contents, antioxidant activity, phenolic compounds, carotenoids contents and colour parameters (L, a, b e ?E). Temperature showed a positive significant (p<0,05) linear effect in the reduction of pectinesterase activity. The best time and temperature ranges for heat processing, which reached more than 95 % of enzyme inactivation were between 20 and 60 seconds at 95?C. The process at 95 ? C/ 60 s promoted a significant decrease in vitamin C content, compared to fresh pulp (Dunnett test at 5% probability level), while the other nutrients did not show significant losses. The presence of coliforms at 45 ?C and Salmonella was not detected. The applied treatments were sufficient to promote a reduction of about 3 logarithmic cycles in the population of molds and yeasts. The Preference Map obtained by the acceptance analyses showed a trend of preference for the pasteurized at 95 ?C / 60 s, 95 ?C / 20 s and fresh samples, while commercial pulps were rejected by consumers.
publishDate 2009
dc.date.issued.fl_str_mv 2009-03-27
dc.date.accessioned.fl_str_mv 2018-08-23T21:04:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv RIBEIRO, Monique Lopes. Efeito do processamento t?rmico nas caracter?sticas f?sico-qu?micas, nutricionais, microbiol?gicas e na atividade enzim?tica de polpa de mam?o Formosa (Carica papaya L.). 2009. 103 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos). Instituto de Tecnologia, Departamento de Tecnologia de Alimentos, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2009.
dc.identifier.uri.fl_str_mv https://tede.ufrrj.br/jspui/handle/jspui/2376
identifier_str_mv RIBEIRO, Monique Lopes. Efeito do processamento t?rmico nas caracter?sticas f?sico-qu?micas, nutricionais, microbiol?gicas e na atividade enzim?tica de polpa de mam?o Formosa (Carica papaya L.). 2009. 103 f. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos). Instituto de Tecnologia, Departamento de Tecnologia de Alimentos, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2009.
url https://tede.ufrrj.br/jspui/handle/jspui/2376
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural do Rio de Janeiro
dc.publisher.program.fl_str_mv Programa de P?s-Gradua??o em Ci?ncia e Tecnologia de Alimentos
dc.publisher.initials.fl_str_mv UFRRJ
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Instituto de Tecnologia
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