Desenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalho

Detalhes bibliográficos
Autor(a) principal: Silva, Adriana dos Santos
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFS
Texto Completo: http://ri.ufs.br/jspui/handle/riufs/16114
Resumo: Curd cheese is a typical product of the Northeast region, produced mainly in artisanal dairies and marketed in open markets, generally deficient in sanitary quality. The objective of this study was the development of an edible coating based on gum arabic and the incorporation of brown propolis from the State of Sergipe, with the purpose of improving the hygienic sanitary quality and useful life of rennet cheese. Physical and chemical characterization analyzes (color analysis, phytochemical screening, phenolic compounds and flavonoids and high performance liquid chromatography) and microbiological analysis (antimicrobial activity of paper disc diffusion and minimal inhibitory concentration by microdilution in broth) were performed. propolis. Coated and coated cheese was elaborated, evaluating the effectiveness of the coating by means of the challenge test technique, its sensorial acceptability and physicochemical characteristics were also evaluated. The results of characterization of propolis used in this research point to a product with high content of flavonoids (> 3.7%) and high sequestering ability of the DPPH radical (97% inhibition), phytochemical screening and chromatographic profile of brown propolis detected phenolic compounds. Also found as well as excellent results of antimicrobial activity against staphylococcus aureus microorganism with inhibition halos up to 27 mm. Microdilution tests in broth showed that the crude extract of propolis presented inhibition power against the bacteria Staphylococcus aureus and Escherichia coli in all the tested concentrations, being the concentration 32 μg / mL, considered as the lowest concentration not only able to inhibit growth , as well as killing bacteria. The challenge test proved the effectiveness of the propolis-coated coating with Sthapylococcus aureus counts in the constant coated cheese, indicating a bacteriostatic action, the opposite was observed in the control cheese, where there was an expressive growth over the 12 days of experiment, with final log 4.0 log cycles longer than the coated cheese sample. The sensory results in relation to the overall assessment, the highest frequency of grades was attributed to grade 8, equivalent to "I liked it a lot". This frequency of notes was maintained during the three cycles of analysis, the subjective analysis of the coating showed a deficiency in homogeneity during the whole evaluation time, this deficiency could be related to the fact that the coating consists of water, and propolis as it is known, has little solubility in water. The physico-chemical parameters of the cheese resemble results reported in the literature.
id UFS-2_1548c561a11817ac3acfa9fbfd93abee
oai_identifier_str oai:ufs.br:riufs/16114
network_acronym_str UFS-2
network_name_str Repositório Institucional da UFS
repository_id_str
spelling Silva, Adriana dos SantosConstant, Patrícia Beltrão LessaNascimento, Ticiano Gomes do2022-08-17T23:44:42Z2022-08-17T23:44:42Z2018-05-30SILVA, Adriana dos Santos. Desenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalho. 2018. 73 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Sergipe, São Cristóvão, 2018.http://ri.ufs.br/jspui/handle/riufs/16114Curd cheese is a typical product of the Northeast region, produced mainly in artisanal dairies and marketed in open markets, generally deficient in sanitary quality. The objective of this study was the development of an edible coating based on gum arabic and the incorporation of brown propolis from the State of Sergipe, with the purpose of improving the hygienic sanitary quality and useful life of rennet cheese. Physical and chemical characterization analyzes (color analysis, phytochemical screening, phenolic compounds and flavonoids and high performance liquid chromatography) and microbiological analysis (antimicrobial activity of paper disc diffusion and minimal inhibitory concentration by microdilution in broth) were performed. propolis. Coated and coated cheese was elaborated, evaluating the effectiveness of the coating by means of the challenge test technique, its sensorial acceptability and physicochemical characteristics were also evaluated. The results of characterization of propolis used in this research point to a product with high content of flavonoids (> 3.7%) and high sequestering ability of the DPPH radical (97% inhibition), phytochemical screening and chromatographic profile of brown propolis detected phenolic compounds. Also found as well as excellent results of antimicrobial activity against staphylococcus aureus microorganism with inhibition halos up to 27 mm. Microdilution tests in broth showed that the crude extract of propolis presented inhibition power against the bacteria Staphylococcus aureus and Escherichia coli in all the tested concentrations, being the concentration 32 μg / mL, considered as the lowest concentration not only able to inhibit growth , as well as killing bacteria. The challenge test proved the effectiveness of the propolis-coated coating with Sthapylococcus aureus counts in the constant coated cheese, indicating a bacteriostatic action, the opposite was observed in the control cheese, where there was an expressive growth over the 12 days of experiment, with final log 4.0 log cycles longer than the coated cheese sample. The sensory results in relation to the overall assessment, the highest frequency of grades was attributed to grade 8, equivalent to "I liked it a lot". This frequency of notes was maintained during the three cycles of analysis, the subjective analysis of the coating showed a deficiency in homogeneity during the whole evaluation time, this deficiency could be related to the fact that the coating consists of water, and propolis as it is known, has little solubility in water. The physico-chemical parameters of the cheese resemble results reported in the literature.O queijo de coalho é um produto típico da região do Nordeste, produzido principalmente em queijarias artesanais e comercializado em feiras livres, geralmente apresenta deficiências na qualidade sanitária. Objetivou estudo o desenvolvido um revestimento comestível a base de goma arábica e com a incorporação de própolis marrom proveniente do estado de Sergipe, com a finalidade de melhor a qualidade higiênico sanitária e vida útil do queijo de coalho. Foram feitas análises de caracterização físico-químicas (análise de cor, screening fitoquimico, teor de compostos fenólicos e flavonoides e cromatografia líquida de alta eficiência) e microbiológica (atividade antimicrobiana de difusão em disco de papel e concentração mínima inibitória por microdiluição em caldo) da própolis. Elaborado o queijo de coalho e revestido, avaliando-se a eficácia do revestimento por meio da técnica do teste desafio, também foi avaliada sua aceitabilidade sensorial e características físico-químicas. Os resultados de caracterização da própolis utilizada nesta pesquisa apontam para um produto com alto teor de flavonóides (> 3,7%) e alta capacidade sequestrante do radical DPPH (97% de inibição), o screening fitoquímico e o perfil cromatográfico da própolis marrom detectaram diversos compostos fenólicos. Também foram encontrados bem como excelentes resultados de atividade antimicrobiana frente ao microrganismo staphylococcus aureus com halos de inibição de até 27 mm. Testes de microdiluição em caldo mostram que o extrato bruto da própolis apresentou poder de inibição frente as bactérias Staphylococcus aureus e Escherichia coli em todas as concentrações testadas, sendo a concentração 32 µg/mL, tida como a menor concentração capaz não só de inibir o crescimento, como também de matar as bactérias. O teste desafio comprovou a eficácia do revestimento incorporado com própolis com valores da contagem de Sthapylococcus aureus no queijo revestido constantes, indicando uma ação bacteriostática, o contrário foi observado no queijo controle, onde houve um crescimento expressivo ao longo dos 12 dias de experimento, com contagem final de 4,0 ciclos log a mais que a amostra de queijo revestido. Os resultados sensórias em relação à avaliação global, a maior frequência de notas foi atribuída a nota 8, equivalente a “gostei muito”. Essa frequência de notas se manteve durante os três ciclos de análises, a análise subjetiva do revestimento houve deficiência na homogeneidade durante todo o tempo de avaliação, essa deficiência pode estar relacionada ao fato do revestimento ser constituído de água, e a própolis como se sabe, apresenta pouca solubilidade em água. Os parâmetros físico-químicos do queijo se assemelham a resultados reportados na literatura.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqSão CristóvãoporQueijo de coalhoRevestimentosFlavonóidesPrópolisRevestimento comestívelPrópolis marromGoma arábicaEdible coatingBrown própolisFlavonoidsGum arabicCurd cheeseCIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSDesenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalhoDevelopment of edible coating incorporated with propolis for use in the conservation of rennet cheeseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPós-Graduação em Ciência e Tecnologia de AlimentosUniversidade Federal de Sergipereponame:Repositório Institucional da UFSinstname:Universidade Federal de Sergipe (UFS)instacron:UFSinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81475https://ri.ufs.br/jspui/bitstream/riufs/16114/1/license.txt098cbbf65c2c15e1fb2e49c5d306a44cMD51ORIGINALADRIANA_SANTOS_SILVA.pdfADRIANA_SANTOS_SILVA.pdfapplication/pdf2029188https://ri.ufs.br/jspui/bitstream/riufs/16114/2/ADRIANA_SANTOS_SILVA.pdf60784272b614e16575aca34fd5081268MD52TEXTADRIANA_SANTOS_SILVA.pdf.txtADRIANA_SANTOS_SILVA.pdf.txtExtracted texttext/plain136678https://ri.ufs.br/jspui/bitstream/riufs/16114/3/ADRIANA_SANTOS_SILVA.pdf.txt72e8be697efa53d0c203175d4e2ecc24MD53THUMBNAILADRIANA_SANTOS_SILVA.pdf.jpgADRIANA_SANTOS_SILVA.pdf.jpgGenerated Thumbnailimage/jpeg1186https://ri.ufs.br/jspui/bitstream/riufs/16114/4/ADRIANA_SANTOS_SILVA.pdf.jpg0495a1d865588de3354d1b76d6d100b2MD54riufs/161142022-08-17 20:44:46.792oai:ufs.br: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Repositório InstitucionalPUBhttps://ri.ufs.br/oai/requestrepositorio@academico.ufs.bropendoar:2022-08-17T23:44:46Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)false
dc.title.pt_BR.fl_str_mv Desenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalho
dc.title.alternative.eng.fl_str_mv Development of edible coating incorporated with propolis for use in the conservation of rennet cheese
title Desenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalho
spellingShingle Desenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalho
Silva, Adriana dos Santos
Queijo de coalho
Revestimentos
Flavonóides
Própolis
Revestimento comestível
Própolis marrom
Goma arábica
Edible coating
Brown própolis
Flavonoids
Gum arabic
Curd cheese
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
title_short Desenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalho
title_full Desenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalho
title_fullStr Desenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalho
title_full_unstemmed Desenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalho
title_sort Desenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalho
author Silva, Adriana dos Santos
author_facet Silva, Adriana dos Santos
author_role author
dc.contributor.author.fl_str_mv Silva, Adriana dos Santos
dc.contributor.advisor1.fl_str_mv Constant, Patrícia Beltrão Lessa
dc.contributor.advisor-co1.fl_str_mv Nascimento, Ticiano Gomes do
contributor_str_mv Constant, Patrícia Beltrão Lessa
Nascimento, Ticiano Gomes do
dc.subject.por.fl_str_mv Queijo de coalho
Revestimentos
Flavonóides
Própolis
Revestimento comestível
Própolis marrom
Goma arábica
topic Queijo de coalho
Revestimentos
Flavonóides
Própolis
Revestimento comestível
Própolis marrom
Goma arábica
Edible coating
Brown própolis
Flavonoids
Gum arabic
Curd cheese
CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
dc.subject.eng.fl_str_mv Edible coating
Brown própolis
Flavonoids
Gum arabic
Curd cheese
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
description Curd cheese is a typical product of the Northeast region, produced mainly in artisanal dairies and marketed in open markets, generally deficient in sanitary quality. The objective of this study was the development of an edible coating based on gum arabic and the incorporation of brown propolis from the State of Sergipe, with the purpose of improving the hygienic sanitary quality and useful life of rennet cheese. Physical and chemical characterization analyzes (color analysis, phytochemical screening, phenolic compounds and flavonoids and high performance liquid chromatography) and microbiological analysis (antimicrobial activity of paper disc diffusion and minimal inhibitory concentration by microdilution in broth) were performed. propolis. Coated and coated cheese was elaborated, evaluating the effectiveness of the coating by means of the challenge test technique, its sensorial acceptability and physicochemical characteristics were also evaluated. The results of characterization of propolis used in this research point to a product with high content of flavonoids (> 3.7%) and high sequestering ability of the DPPH radical (97% inhibition), phytochemical screening and chromatographic profile of brown propolis detected phenolic compounds. Also found as well as excellent results of antimicrobial activity against staphylococcus aureus microorganism with inhibition halos up to 27 mm. Microdilution tests in broth showed that the crude extract of propolis presented inhibition power against the bacteria Staphylococcus aureus and Escherichia coli in all the tested concentrations, being the concentration 32 μg / mL, considered as the lowest concentration not only able to inhibit growth , as well as killing bacteria. The challenge test proved the effectiveness of the propolis-coated coating with Sthapylococcus aureus counts in the constant coated cheese, indicating a bacteriostatic action, the opposite was observed in the control cheese, where there was an expressive growth over the 12 days of experiment, with final log 4.0 log cycles longer than the coated cheese sample. The sensory results in relation to the overall assessment, the highest frequency of grades was attributed to grade 8, equivalent to "I liked it a lot". This frequency of notes was maintained during the three cycles of analysis, the subjective analysis of the coating showed a deficiency in homogeneity during the whole evaluation time, this deficiency could be related to the fact that the coating consists of water, and propolis as it is known, has little solubility in water. The physico-chemical parameters of the cheese resemble results reported in the literature.
publishDate 2018
dc.date.issued.fl_str_mv 2018-05-30
dc.date.accessioned.fl_str_mv 2022-08-17T23:44:42Z
dc.date.available.fl_str_mv 2022-08-17T23:44:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Adriana dos Santos. Desenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalho. 2018. 73 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Sergipe, São Cristóvão, 2018.
dc.identifier.uri.fl_str_mv http://ri.ufs.br/jspui/handle/riufs/16114
identifier_str_mv SILVA, Adriana dos Santos. Desenvolvimento de revestimento comestível incorporado com própolis para uso na conservação de queijo de coalho. 2018. 73 f. Dissertação (Mestrado em Ciência e Tecnologia de Alimentos) – Universidade Federal de Sergipe, São Cristóvão, 2018.
url http://ri.ufs.br/jspui/handle/riufs/16114
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.program.fl_str_mv Pós-Graduação em Ciência e Tecnologia de Alimentos
dc.publisher.initials.fl_str_mv Universidade Federal de Sergipe
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFS
instname:Universidade Federal de Sergipe (UFS)
instacron:UFS
instname_str Universidade Federal de Sergipe (UFS)
instacron_str UFS
institution UFS
reponame_str Repositório Institucional da UFS
collection Repositório Institucional da UFS
bitstream.url.fl_str_mv https://ri.ufs.br/jspui/bitstream/riufs/16114/1/license.txt
https://ri.ufs.br/jspui/bitstream/riufs/16114/2/ADRIANA_SANTOS_SILVA.pdf
https://ri.ufs.br/jspui/bitstream/riufs/16114/3/ADRIANA_SANTOS_SILVA.pdf.txt
https://ri.ufs.br/jspui/bitstream/riufs/16114/4/ADRIANA_SANTOS_SILVA.pdf.jpg
bitstream.checksum.fl_str_mv 098cbbf65c2c15e1fb2e49c5d306a44c
60784272b614e16575aca34fd5081268
72e8be697efa53d0c203175d4e2ecc24
0495a1d865588de3354d1b76d6d100b2
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFS - Universidade Federal de Sergipe (UFS)
repository.mail.fl_str_mv repositorio@academico.ufs.br
_version_ 1802110674884624384