Heterologous primer transferability and access to microsatellite loci polymorphism in ‘somnus’ passion fruit tree (Passiflora setacea DC)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Biotemas (Online) |
Texto Completo: | https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n3p51 |
Resumo: | Primer pairs that access microsatellite loci, initially constructed through the genome of Passiflora edulis Sims flavicarpa and P. alata, were tested concerning their ability to access microsatellite loci in ‘somnus’ passion fruit tree (P. setacea) individuals. Seven out of the thirty one primer pairs tested were able to access DNA polymorphism in the genome of this wild Passiflora species, by evaluating six natural populations, located in a transition area between the biomes Caatinga and Cerrado, in the state of Bahia, Brazil. The number of alleles/loci was small, oscillating from 1 to 4. The average heterozygosity observed per locus in all populations ranged from 0.13 to 0.40. There was transference of heterologous microsatellite primer pairs from the Passiflora genus to ‘somnus’ passion fruit tree, constituting a new set of primers that access random co-dominant locus in this species, useful for conservationist purposes and pre-improvement of ‘somnus’ passion fruit tree. |
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Heterologous primer transferability and access to microsatellite loci polymorphism in ‘somnus’ passion fruit tree (Passiflora setacea DC)Transferibilidade de primers heterólogos e acesso ao polimorfismo de loci microssatélites em maracujazeiro-do-sono (Passiflora setacea DC)Primer pairs that access microsatellite loci, initially constructed through the genome of Passiflora edulis Sims flavicarpa and P. alata, were tested concerning their ability to access microsatellite loci in ‘somnus’ passion fruit tree (P. setacea) individuals. Seven out of the thirty one primer pairs tested were able to access DNA polymorphism in the genome of this wild Passiflora species, by evaluating six natural populations, located in a transition area between the biomes Caatinga and Cerrado, in the state of Bahia, Brazil. The number of alleles/loci was small, oscillating from 1 to 4. The average heterozygosity observed per locus in all populations ranged from 0.13 to 0.40. There was transference of heterologous microsatellite primer pairs from the Passiflora genus to ‘somnus’ passion fruit tree, constituting a new set of primers that access random co-dominant locus in this species, useful for conservationist purposes and pre-improvement of ‘somnus’ passion fruit tree. Pares de primers que acessam loci microssatélites, inicialmente construídos a partir do genoma de Passiflora edulis Sims flavicarpa e P. alata, foram testados quanto à sua capacidade de acessar loci microssatélites em indivíduos de maracujazeiro-do-sono (P. setacea). Sete dos trinta e um pares de primers testados foram capazes de acessar polimorfismo de DNA no genoma dessa espécie silvestre de Passiflora, ao avaliar seis populações naturais, localizadas em área de transição entre os biomas Caatinga e Cerrado, no estado da Bahia. O número de alelos/loci foi baixo, oscilando de um a quatro. A heterozigosidade média observada por locus em todas as populações variou de 0,13 a 0,40. Houve transferência dos pares de primers microssatélites heterólogos do gênero Passiflora para maracujazeiro-do-sono, constituindo um novo conjunto de primers que acessam locus codominante aleatório nessa espécie, útil para fins de conservação e pré-melhoramento do maracujazeiro-do-sono.Universidade Federal de Santa Catarina2015-04-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n3p5110.5007/2175-7925.2015v28n3p51Biotemas; v. 28 n. 3 (2015); 51-562175-79250103-1643reponame:Biotemas (Online)instname:Universidade Federal de Santa Catarina (UFSC)instacron:UFSCenghttps://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n3p51/29977Copyright (c) 2015 Douglas de Almeida Pereira, Fernanda Amato Gaiotto, Ronan Xavier Corrêa, Antonio Carlos de Oliveirainfo:eu-repo/semantics/openAccessPereira, Douglas de AlmeidaGaiotto, Fernanda AmatoCorrêa, Ronan Xavierde Oliveira, Antonio Carlos2017-10-19T16:05:22Zoai:periodicos.ufsc.br:article/34331Revistahttp://www.biotemas.ufsc.br/index.htmPUBhttps://periodicos.ufsc.br/index.php/biotemas/oai||carlospinto@ccb.ufsc.br2175-79250103-1643opendoar:2017-10-19T16:05:22Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC)false |
dc.title.none.fl_str_mv |
Heterologous primer transferability and access to microsatellite loci polymorphism in ‘somnus’ passion fruit tree (Passiflora setacea DC) Transferibilidade de primers heterólogos e acesso ao polimorfismo de loci microssatélites em maracujazeiro-do-sono (Passiflora setacea DC) |
title |
Heterologous primer transferability and access to microsatellite loci polymorphism in ‘somnus’ passion fruit tree (Passiflora setacea DC) |
spellingShingle |
Heterologous primer transferability and access to microsatellite loci polymorphism in ‘somnus’ passion fruit tree (Passiflora setacea DC) Pereira, Douglas de Almeida |
title_short |
Heterologous primer transferability and access to microsatellite loci polymorphism in ‘somnus’ passion fruit tree (Passiflora setacea DC) |
title_full |
Heterologous primer transferability and access to microsatellite loci polymorphism in ‘somnus’ passion fruit tree (Passiflora setacea DC) |
title_fullStr |
Heterologous primer transferability and access to microsatellite loci polymorphism in ‘somnus’ passion fruit tree (Passiflora setacea DC) |
title_full_unstemmed |
Heterologous primer transferability and access to microsatellite loci polymorphism in ‘somnus’ passion fruit tree (Passiflora setacea DC) |
title_sort |
Heterologous primer transferability and access to microsatellite loci polymorphism in ‘somnus’ passion fruit tree (Passiflora setacea DC) |
author |
Pereira, Douglas de Almeida |
author_facet |
Pereira, Douglas de Almeida Gaiotto, Fernanda Amato Corrêa, Ronan Xavier de Oliveira, Antonio Carlos |
author_role |
author |
author2 |
Gaiotto, Fernanda Amato Corrêa, Ronan Xavier de Oliveira, Antonio Carlos |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pereira, Douglas de Almeida Gaiotto, Fernanda Amato Corrêa, Ronan Xavier de Oliveira, Antonio Carlos |
description |
Primer pairs that access microsatellite loci, initially constructed through the genome of Passiflora edulis Sims flavicarpa and P. alata, were tested concerning their ability to access microsatellite loci in ‘somnus’ passion fruit tree (P. setacea) individuals. Seven out of the thirty one primer pairs tested were able to access DNA polymorphism in the genome of this wild Passiflora species, by evaluating six natural populations, located in a transition area between the biomes Caatinga and Cerrado, in the state of Bahia, Brazil. The number of alleles/loci was small, oscillating from 1 to 4. The average heterozygosity observed per locus in all populations ranged from 0.13 to 0.40. There was transference of heterologous microsatellite primer pairs from the Passiflora genus to ‘somnus’ passion fruit tree, constituting a new set of primers that access random co-dominant locus in this species, useful for conservationist purposes and pre-improvement of ‘somnus’ passion fruit tree. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-04-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n3p51 10.5007/2175-7925.2015v28n3p51 |
url |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n3p51 |
identifier_str_mv |
10.5007/2175-7925.2015v28n3p51 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.ufsc.br/index.php/biotemas/article/view/2175-7925.2015v28n3p51/29977 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
publisher.none.fl_str_mv |
Universidade Federal de Santa Catarina |
dc.source.none.fl_str_mv |
Biotemas; v. 28 n. 3 (2015); 51-56 2175-7925 0103-1643 reponame:Biotemas (Online) instname:Universidade Federal de Santa Catarina (UFSC) instacron:UFSC |
instname_str |
Universidade Federal de Santa Catarina (UFSC) |
instacron_str |
UFSC |
institution |
UFSC |
reponame_str |
Biotemas (Online) |
collection |
Biotemas (Online) |
repository.name.fl_str_mv |
Biotemas (Online) - Universidade Federal de Santa Catarina (UFSC) |
repository.mail.fl_str_mv |
||carlospinto@ccb.ufsc.br |
_version_ |
1799940553493708800 |