Unraveling the role of high-intensity resistance training on left ventricle proteome: Is there a shift towards maladaptation?
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/55979 http://dx.doi.org/10.1016/j.lfs.2016.03.040 |
Resumo: | High-intensity resistance training (RT) induces adaptations that improve physiological function. However, high intensity, volume and/or frequency may lead to injury and other health issues such as adverse cardiac effects. The aim of this study was to evaluate the effect of RT on left ventricle proteome, and to identify the pathways involved on the harmful adaptations induced by this protocol. Male Wistar rats were randomized into 2 groups: Trained (T) and Sedentary (S). Animals from T group were trained for 6 weeks, and then all the animals were sacrificed and left ventricle was isolated for analysis. We identified 955 proteins, and 93 proteins were considered |
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Dantas, Patricia Sousa [UNIFESP]Sakata, Maisa Mayumi [UNIFESP]Perez, Juliana Dineia [UNIFESP]Watanabe, Regina Lucia Harumi [UNIFESP]Bizerra, Fernando Cesar [UNIFESP]Neves, Vander Jose dasGuzzoni, ViniciusMarcondes, Fernanda KleinCasarini, Dulce Elena [UNIFESP]Cunha, Tatiana Sousa [UNIFESP]2020-07-22T13:23:00Z2020-07-22T13:23:00Z2016Life Sciences. Oxford, v. 152, p. 156-164, 2016.0024-3205https://repositorio.unifesp.br/handle/11600/55979http://dx.doi.org/10.1016/j.lfs.2016.03.04010.1016/j.lfs.2016.03.040WOS:000375728500019High-intensity resistance training (RT) induces adaptations that improve physiological function. However, high intensity, volume and/or frequency may lead to injury and other health issues such as adverse cardiac effects. The aim of this study was to evaluate the effect of RT on left ventricle proteome, and to identify the pathways involved on the harmful adaptations induced by this protocol. Male Wistar rats were randomized into 2 groups: Trained (T) and Sedentary (S). Animals from T group were trained for 6 weeks, and then all the animals were sacrificed and left ventricle was isolated for analysis. We identified 955 proteins, and 93 proteins were considered36 were expressed exclusively in T group, and 4 in S group. Based on quantitative analysis, 42 proteins were found overexpressed and 11 underexpressed in T group compared with S group. Using the Gene Ontology to relate the biological processes in which these proteins are involved, we conclude that RT protocol promotes changes similar to those found in the initial phase of heart failure, but we also observed a concomitant increased expression of protective proteins, suggesting the activation of pathways to avoid major damages on left ventricle and delay the onset of pathological hypertrophy. Statement of significance of the study: Our study shows that high-intensity RT protocol changes left ventricle proteome, modifying metabolic profile of heart tissue and inducing the expression of proteins that acts against cardiac injury. We hypothesize that these adaptations occur to prevent the onset of cardiac dysfunction. Despite highly significant, it remains to be determined whether these adaptations are sufficient to further keep left ventricle function and exert cardioprotection, and whether this panel will be shifted towards maladaptation, and heart failure. (c) 2016 Elsevier Inc. All rights reserved.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESPFAEP/UNICAMPCAPES, BrazilFed Univ Sao Paulo UNIFESP, Dept Med, Discipline Nephrol, Sao Paulo, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Physiol, Discipline Nutr Physiol, Sao Paulo, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Med, Discipline Infect Dis, Special Mycol Lab LEMI, Sao Paulo, SP, BrazilUniv Estadual Campinas, Fac Dent Piracicaba FOP UNICAMP, Dept Physiol Sci, Piracicaba, SP, BrazilFed Univ Sao Paulo UNIFESP, Inst Sci & Technol, Dept Sci & Technol, BR-12231280 Sao Jose Dos Campos, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Med, Discipline Nephrol, Sao Paulo, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Physiol, Discipline Nutr Physiol, Sao Paulo, SP, BrazilFed Univ Sao Paulo UNIFESP, Dept Med, Discipline Infect Dis, Special Mycol Lab LEMI, Sao Paulo, SP, BrazilFed Univ Sao Paulo UNIFESP, Inst Sci & Technol, Dept Sci & Technol, BR-12231280 Sao Jose Dos Campos, SP, BrazilFAPESP: 02/05427-8FAEP/UNICAMP: 398/03FAEP/UNICAMP: 680/03Web of Science156-164engPergamon-Elsevier Science LtdLife SciencesProteomics shotgunRat proteomicsHigh-intensity resistance trainingCardiac hypertrophyPathological hypertrophyUnraveling the role of high-intensity resistance training on left ventricle proteome: Is there a shift towards maladaptation?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleOxford152info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/559792021-09-29 09:32:03.721metadata only accessoai:repositorio.unifesp.br:11600/55979Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-09-29T12:32:03Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Unraveling the role of high-intensity resistance training on left ventricle proteome: Is there a shift towards maladaptation? |
title |
Unraveling the role of high-intensity resistance training on left ventricle proteome: Is there a shift towards maladaptation? |
spellingShingle |
Unraveling the role of high-intensity resistance training on left ventricle proteome: Is there a shift towards maladaptation? Dantas, Patricia Sousa [UNIFESP] Proteomics shotgun Rat proteomics High-intensity resistance training Cardiac hypertrophy Pathological hypertrophy |
title_short |
Unraveling the role of high-intensity resistance training on left ventricle proteome: Is there a shift towards maladaptation? |
title_full |
Unraveling the role of high-intensity resistance training on left ventricle proteome: Is there a shift towards maladaptation? |
title_fullStr |
Unraveling the role of high-intensity resistance training on left ventricle proteome: Is there a shift towards maladaptation? |
title_full_unstemmed |
Unraveling the role of high-intensity resistance training on left ventricle proteome: Is there a shift towards maladaptation? |
title_sort |
Unraveling the role of high-intensity resistance training on left ventricle proteome: Is there a shift towards maladaptation? |
author |
Dantas, Patricia Sousa [UNIFESP] |
author_facet |
Dantas, Patricia Sousa [UNIFESP] Sakata, Maisa Mayumi [UNIFESP] Perez, Juliana Dineia [UNIFESP] Watanabe, Regina Lucia Harumi [UNIFESP] Bizerra, Fernando Cesar [UNIFESP] Neves, Vander Jose das Guzzoni, Vinicius Marcondes, Fernanda Klein Casarini, Dulce Elena [UNIFESP] Cunha, Tatiana Sousa [UNIFESP] |
author_role |
author |
author2 |
Sakata, Maisa Mayumi [UNIFESP] Perez, Juliana Dineia [UNIFESP] Watanabe, Regina Lucia Harumi [UNIFESP] Bizerra, Fernando Cesar [UNIFESP] Neves, Vander Jose das Guzzoni, Vinicius Marcondes, Fernanda Klein Casarini, Dulce Elena [UNIFESP] Cunha, Tatiana Sousa [UNIFESP] |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Dantas, Patricia Sousa [UNIFESP] Sakata, Maisa Mayumi [UNIFESP] Perez, Juliana Dineia [UNIFESP] Watanabe, Regina Lucia Harumi [UNIFESP] Bizerra, Fernando Cesar [UNIFESP] Neves, Vander Jose das Guzzoni, Vinicius Marcondes, Fernanda Klein Casarini, Dulce Elena [UNIFESP] Cunha, Tatiana Sousa [UNIFESP] |
dc.subject.eng.fl_str_mv |
Proteomics shotgun Rat proteomics High-intensity resistance training Cardiac hypertrophy Pathological hypertrophy |
topic |
Proteomics shotgun Rat proteomics High-intensity resistance training Cardiac hypertrophy Pathological hypertrophy |
description |
High-intensity resistance training (RT) induces adaptations that improve physiological function. However, high intensity, volume and/or frequency may lead to injury and other health issues such as adverse cardiac effects. The aim of this study was to evaluate the effect of RT on left ventricle proteome, and to identify the pathways involved on the harmful adaptations induced by this protocol. Male Wistar rats were randomized into 2 groups: Trained (T) and Sedentary (S). Animals from T group were trained for 6 weeks, and then all the animals were sacrificed and left ventricle was isolated for analysis. We identified 955 proteins, and 93 proteins were considered |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-07-22T13:23:00Z |
dc.date.available.fl_str_mv |
2020-07-22T13:23:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Life Sciences. Oxford, v. 152, p. 156-164, 2016. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/55979 http://dx.doi.org/10.1016/j.lfs.2016.03.040 |
dc.identifier.issn.none.fl_str_mv |
0024-3205 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.lfs.2016.03.040 |
dc.identifier.wos.none.fl_str_mv |
WOS:000375728500019 |
identifier_str_mv |
Life Sciences. Oxford, v. 152, p. 156-164, 2016. 0024-3205 10.1016/j.lfs.2016.03.040 WOS:000375728500019 |
url |
https://repositorio.unifesp.br/handle/11600/55979 http://dx.doi.org/10.1016/j.lfs.2016.03.040 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Life Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
156-164 |
dc.coverage.none.fl_str_mv |
Oxford |
dc.publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
publisher.none.fl_str_mv |
Pergamon-Elsevier Science Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1802764152800804864 |