Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/38759 http://dx.doi.org/10.1371/journal.pone.0117778 |
Resumo: | Targeting antigens to dendritic cells (DCs) by using hybrid monoclonal antibodies (mAbs) directed against DC receptors is known to improve activation and support long-lasting T cell responses. in the present work, we used the mAb alpha DEC205 fused to the Trypanosoma cruzi amastigote surface protein 2 (ASP-2) to identify a region of this protein recognized by specific T cells. the hybrid alpha DEC-ASP2 mAb was successfully generated and preserved its ability to bind the DEC205 receptor. Immunization of BALB/c mice with the recombinant mAb in the presence of polyriboinosinic: polyribocytidylic acid (poly (I:C)) specifically enhanced the number of IFN-gamma producing cells and CD4+ T cell proliferation when compared to mice immunized with a mAb without receptor affinity or with the non-targeted ASP-2 protein. the strong immune response induced in mice immunized with the hybrid alpha DEC-ASP2 mAb allowed us to identify an ASP-2-specific CD4+ T cell epitope recognized by the BALB/c MHCII haplotype. We conclude that targeting parasite antigens to DCs is a useful strategy to enhance T cell mediated immune responses facilitating the identification of new T-cell epitopes. |
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Rampazo, Eline V.Amorim, Kelly N. S.Yamamoto, Marcio M.Panatieri, Raquel HoffmannRodrigues, Mauricio Martins [UNIFESP]Boscardin, Silvia B.Universidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Natl Inst Sci & Technol Vaccines2016-01-24T14:40:04Z2016-01-24T14:40:04Z2015-02-13Plos One. San Francisco: Public Library Science, v. 10, n. 2, 13 p., 2015.1932-6203http://repositorio.unifesp.br/handle/11600/38759http://dx.doi.org/10.1371/journal.pone.0117778WOS000350682600087.pdf10.1371/journal.pone.0117778WOS:000350682600087Targeting antigens to dendritic cells (DCs) by using hybrid monoclonal antibodies (mAbs) directed against DC receptors is known to improve activation and support long-lasting T cell responses. in the present work, we used the mAb alpha DEC205 fused to the Trypanosoma cruzi amastigote surface protein 2 (ASP-2) to identify a region of this protein recognized by specific T cells. the hybrid alpha DEC-ASP2 mAb was successfully generated and preserved its ability to bind the DEC205 receptor. Immunization of BALB/c mice with the recombinant mAb in the presence of polyriboinosinic: polyribocytidylic acid (poly (I:C)) specifically enhanced the number of IFN-gamma producing cells and CD4+ T cell proliferation when compared to mice immunized with a mAb without receptor affinity or with the non-targeted ASP-2 protein. the strong immune response induced in mice immunized with the hybrid alpha DEC-ASP2 mAb allowed us to identify an ASP-2-specific CD4+ T cell epitope recognized by the BALB/c MHCII haplotype. We conclude that targeting parasite antigens to DCs is a useful strategy to enhance T cell mediated immune responses facilitating the identification of new T-cell epitopes.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)BNP-Paribas Bankcommercial source BNP-Paribas BankUniv São Paulo, Inst Biomed Sci, Dept Parasitol, Lab Antigen Targeting Dendrit Cells, São Paulo, BrazilUniversidade Federal de São Paulo, CTCMol, São Paulo, BrazilNatl Inst Sci & Technol Vaccines, Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, CTCMol, São Paulo, BrazilCNPq: 15203*12FAPESP: 2007/08648-9Web of Science13engPublic Library SciencePlos OneAntigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigeninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000350682600087.pdfapplication/pdf620969${dspace.ui.url}/bitstream/11600/38759/1/WOS000350682600087.pdff5f7b4de1e7b2cf6da2c0a286f47b9fbMD51open accessTEXTWOS000350682600087.pdf.txtWOS000350682600087.pdf.txtExtracted texttext/plain41424${dspace.ui.url}/bitstream/11600/38759/2/WOS000350682600087.pdf.txta39a8921c003efdce2a22e2c6cb53ca4MD52open access11600/387592022-11-03 15:00:28.059open accessoai:repositorio.unifesp.br:11600/38759Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-11-03T18:00:28Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
title |
Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
spellingShingle |
Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen Rampazo, Eline V. |
title_short |
Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
title_full |
Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
title_fullStr |
Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
title_full_unstemmed |
Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
title_sort |
Antigen Targeting to Dendritic Cells Allows the Identification of a CD4 T-Cell Epitope within an Immunodominant Trypanosoma cruzi Antigen |
author |
Rampazo, Eline V. |
author_facet |
Rampazo, Eline V. Amorim, Kelly N. S. Yamamoto, Marcio M. Panatieri, Raquel Hoffmann Rodrigues, Mauricio Martins [UNIFESP] Boscardin, Silvia B. |
author_role |
author |
author2 |
Amorim, Kelly N. S. Yamamoto, Marcio M. Panatieri, Raquel Hoffmann Rodrigues, Mauricio Martins [UNIFESP] Boscardin, Silvia B. |
author2_role |
author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) Natl Inst Sci & Technol Vaccines |
dc.contributor.author.fl_str_mv |
Rampazo, Eline V. Amorim, Kelly N. S. Yamamoto, Marcio M. Panatieri, Raquel Hoffmann Rodrigues, Mauricio Martins [UNIFESP] Boscardin, Silvia B. |
description |
Targeting antigens to dendritic cells (DCs) by using hybrid monoclonal antibodies (mAbs) directed against DC receptors is known to improve activation and support long-lasting T cell responses. in the present work, we used the mAb alpha DEC205 fused to the Trypanosoma cruzi amastigote surface protein 2 (ASP-2) to identify a region of this protein recognized by specific T cells. the hybrid alpha DEC-ASP2 mAb was successfully generated and preserved its ability to bind the DEC205 receptor. Immunization of BALB/c mice with the recombinant mAb in the presence of polyriboinosinic: polyribocytidylic acid (poly (I:C)) specifically enhanced the number of IFN-gamma producing cells and CD4+ T cell proliferation when compared to mice immunized with a mAb without receptor affinity or with the non-targeted ASP-2 protein. the strong immune response induced in mice immunized with the hybrid alpha DEC-ASP2 mAb allowed us to identify an ASP-2-specific CD4+ T cell epitope recognized by the BALB/c MHCII haplotype. We conclude that targeting parasite antigens to DCs is a useful strategy to enhance T cell mediated immune responses facilitating the identification of new T-cell epitopes. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-13 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:40:04Z |
dc.date.available.fl_str_mv |
2016-01-24T14:40:04Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Plos One. San Francisco: Public Library Science, v. 10, n. 2, 13 p., 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/38759 http://dx.doi.org/10.1371/journal.pone.0117778 |
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1932-6203 |
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WOS000350682600087.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0117778 |
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WOS:000350682600087 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 10, n. 2, 13 p., 2015. 1932-6203 WOS000350682600087.pdf 10.1371/journal.pone.0117778 WOS:000350682600087 |
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http://repositorio.unifesp.br/handle/11600/38759 http://dx.doi.org/10.1371/journal.pone.0117778 |
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