High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil

Detalhes bibliográficos
Autor(a) principal: Spano, Liliana Cruz
Data de Publicação: 2017
Outros Autores: da Cunha, Keyla Fonseca, Monfardini, Mariane Vedovatti [UNIFESP], Bergamaschi Fonseca, Rita de Cassia, Affonso Scaletsky, Isabel Christina [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1186/s12879-017-2872-0
https://repositorio.unifesp.br/handle/11600/53989
Resumo: Background: Diarrheagenic Escherichia coli (DEC) are important bacterial causes of childhood diarrhea in Brazil, but its impact in adults is unknown. This study aimed at investigating DEC among children and adults living in endemic areas. Methods: A total of 327 stools specimens were collected from children (n = 141) and adults (n = 186) with diarrhea attending health centers. Diarrheagenic E. coli (DEC) were identified by their virulence genes (multiplex polymerase chain reaction) and HEp-2 cell adherence patterns. Results: DEC were detected in 56 (40%) children and 74 (39%) adults
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spelling High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern BrazilDiarrheagenic E. coliChildrenAdultsBackground: Diarrheagenic Escherichia coli (DEC) are important bacterial causes of childhood diarrhea in Brazil, but its impact in adults is unknown. This study aimed at investigating DEC among children and adults living in endemic areas. Methods: A total of 327 stools specimens were collected from children (n = 141) and adults (n = 186) with diarrhea attending health centers. Diarrheagenic E. coli (DEC) were identified by their virulence genes (multiplex polymerase chain reaction) and HEp-2 cell adherence patterns. Results: DEC were detected in 56 (40%) children and 74 (39%) adultsenteroaggregative E. coli (EAEC) (23%) was the most prevalent pathotype, followed by diffusely adherent E. coli (DAEC) (13%), and occurred at similar frequencies in both diarrheal groups. Atypical enteropathogenic E. coli (aEPEC) strains were recovered more frequently from children (6%) than from adults (1%). Twenty-six percent of the EAEC were classified as typical EAEC possessing aggR gene, and carried the aap gene. EAEC strains carrying aggR-aap-aatA genes were significantly more frequent among children than adults (p < 0.05). DAEC strains possessing Afa/Dr. genes were detected from children (10%) and adults (6%). EAEC and DAEC strains harboring genes for the EAST1 (astA), Pet, Pic, and Sat toxins were common in both diarrheal groups. The astA and the porcine AE/associated adhesin (paa) genes were found in most of aEPEC strains. High levels of resistance to antimicrobial drugs were found among DAEC and aEPEC isolates. Conclusion: The results show a high proportion of EAEC and DAEC carrying toxin-encoding genes among adults with diarrhea.Univ Fed Espirito Santo, Ctr Ciencias Saude, Dept Patol, Lab Virol & Gastrenterite Infecciosa, Ave Marechal Campos 1468, BR-29043900 Vitoria, ES, BrazilUniv Fed Espirito Santo, Nucleo Doencas Infecciosas, Dept Med Social, Ctr Ciencias Saude, Vitoria, ES, BrazilUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Vitoria, ES, BrazilLab Cent Municipal, Vitoria, ES, BrazilUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Vitoria, ES, BrazilWeb of ScienceFoundation for Science and Technology of Victoria Municipality (FACITEC) of Espirito Santo State, BrazilBiomed Central Ltd2020-07-02T18:52:16Z2020-07-02T18:52:16Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion-application/pdfhttp://dx.doi.org/10.1186/s12879-017-2872-0Bmc Infectious Diseases. London, v. 17, p. -, 2017.10.1186/s12879-017-2872-0WOS000418160300001.pdf1471-2334https://repositorio.unifesp.br/handle/11600/53989WOS:000418160300001engBmc Infectious DiseasesLondoninfo:eu-repo/semantics/openAccessSpano, Liliana Cruzda Cunha, Keyla FonsecaMonfardini, Mariane Vedovatti [UNIFESP]Bergamaschi Fonseca, Rita de CassiaAffonso Scaletsky, Isabel Christina [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-03T05:24:16Zoai:repositorio.unifesp.br/:11600/53989Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-03T05:24:16Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
title High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
spellingShingle High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
Spano, Liliana Cruz
Diarrheagenic E. coli
Children
Adults
title_short High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
title_full High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
title_fullStr High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
title_full_unstemmed High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
title_sort High prevalence of diarrheagenic Escherichia coli carrying toxin-encoding genes isolated from children and adults in southeastern Brazil
author Spano, Liliana Cruz
author_facet Spano, Liliana Cruz
da Cunha, Keyla Fonseca
Monfardini, Mariane Vedovatti [UNIFESP]
Bergamaschi Fonseca, Rita de Cassia
Affonso Scaletsky, Isabel Christina [UNIFESP]
author_role author
author2 da Cunha, Keyla Fonseca
Monfardini, Mariane Vedovatti [UNIFESP]
Bergamaschi Fonseca, Rita de Cassia
Affonso Scaletsky, Isabel Christina [UNIFESP]
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Spano, Liliana Cruz
da Cunha, Keyla Fonseca
Monfardini, Mariane Vedovatti [UNIFESP]
Bergamaschi Fonseca, Rita de Cassia
Affonso Scaletsky, Isabel Christina [UNIFESP]
dc.subject.por.fl_str_mv Diarrheagenic E. coli
Children
Adults
topic Diarrheagenic E. coli
Children
Adults
description Background: Diarrheagenic Escherichia coli (DEC) are important bacterial causes of childhood diarrhea in Brazil, but its impact in adults is unknown. This study aimed at investigating DEC among children and adults living in endemic areas. Methods: A total of 327 stools specimens were collected from children (n = 141) and adults (n = 186) with diarrhea attending health centers. Diarrheagenic E. coli (DEC) were identified by their virulence genes (multiplex polymerase chain reaction) and HEp-2 cell adherence patterns. Results: DEC were detected in 56 (40%) children and 74 (39%) adults
publishDate 2017
dc.date.none.fl_str_mv 2017
2020-07-02T18:52:16Z
2020-07-02T18:52:16Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s12879-017-2872-0
Bmc Infectious Diseases. London, v. 17, p. -, 2017.
10.1186/s12879-017-2872-0
WOS000418160300001.pdf
1471-2334
https://repositorio.unifesp.br/handle/11600/53989
WOS:000418160300001
url http://dx.doi.org/10.1186/s12879-017-2872-0
https://repositorio.unifesp.br/handle/11600/53989
identifier_str_mv Bmc Infectious Diseases. London, v. 17, p. -, 2017.
10.1186/s12879-017-2872-0
WOS000418160300001.pdf
1471-2334
WOS:000418160300001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bmc Infectious Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.coverage.none.fl_str_mv London
dc.publisher.none.fl_str_mv Biomed Central Ltd
publisher.none.fl_str_mv Biomed Central Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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