Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response

Detalhes bibliográficos
Autor(a) principal: Larocca, Rafael Assumpcao
Data de Publicação: 2013
Outros Autores: Moraes-Vieira, Pedro Manoel, Bassi, Enio Jose, Semedo, Patricia [UNIFESP], Almeida, Danilo Candido de [UNIFESP], Silva, Marina Burgos da, Thornley, Thomas, Pacheco-Silva, Alvaro [UNIFESP], Câmara, Niels Olsen Saraiva [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0076396
http://repositorio.unifesp.br/handle/11600/36868
Resumo: Adipose tissue-derived mesenchymal stem cells (ADSC) exhibit immunosuppressive capabilities both in vitro and in vivo. Their use for therapy in the transplant field is attractive as they could render the use of immunosuppressive drugs unnecessary. the aim of this study was to investigate the effect of ADSC therapy on prolonging skin allograft survival. Animals that were treated with a single injection of donor allogeneic ADSC one day after transplantation showed an increase in donor skin graft survival by approximately one week. This improvement was associated with preserved histological morphology, an expansion of CD4(+) regulatory T cells (Treg) in draining lymph nodes, as well as heightened IL-10 expression and down-regulated IL-17 expression. in vitro, ADSC inhibit naive CD4(+) T cell proliferation and constrain Th-1 and Th-17 polarization. in summary, infusion of ADSC one day post-transplantation dramatically increases skin allograft survival by inhibiting the Th-17 pathogenic immune response and enhancing the protective Treg immune response. Finally, these data suggest that ADSC therapy will open new opportunities for promoting drug-free allograft survival in clinical transplantation.
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spelling Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune ResponseAdipose tissue-derived mesenchymal stem cells (ADSC) exhibit immunosuppressive capabilities both in vitro and in vivo. Their use for therapy in the transplant field is attractive as they could render the use of immunosuppressive drugs unnecessary. the aim of this study was to investigate the effect of ADSC therapy on prolonging skin allograft survival. Animals that were treated with a single injection of donor allogeneic ADSC one day after transplantation showed an increase in donor skin graft survival by approximately one week. This improvement was associated with preserved histological morphology, an expansion of CD4(+) regulatory T cells (Treg) in draining lymph nodes, as well as heightened IL-10 expression and down-regulated IL-17 expression. in vitro, ADSC inhibit naive CD4(+) T cell proliferation and constrain Th-1 and Th-17 polarization. in summary, infusion of ADSC one day post-transplantation dramatically increases skin allograft survival by inhibiting the Th-17 pathogenic immune response and enhancing the protective Treg immune response. Finally, these data suggest that ADSC therapy will open new opportunities for promoting drug-free allograft survival in clinical transplantation.Univ São Paulo, Lab Transplantat Immunobiol, Dept Immunol, Inst Biomed Sci, São Paulo, BrazilUniversidade Federal de São Paulo, Lab Clin & Expt Immunol, Div Nephrol, São Paulo, BrazilAlbert Einstein Hosp, Inst Israelita Ensino & Pesquisa, Renal Transplantat Div, São Paulo, BrazilHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Dept Med,Transplant Inst, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Dept Med,Div Endocrinol, Boston, MA 02215 USAUniversidade Federal de São Paulo, Lab Clin & Expt Immunol, Div Nephrol, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian Council of Scientific and Technologic DevelopmentComplex Fluids INCT, Coordination of Improvement of Higher Education PersonnelFAPESP: 07/07139-3FAPESP: 06/55326-4FAPESP: 10/52180-4FAPESP: 12/02270-2Brazilian Council of Scientific and Technologic Development: 470533/2007-2Brazilian Council of Scientific and Technologic Development: CNPq/DECIT/MSComplex Fluids INCT, Coordination of Improvement of Higher Education Personnel: CAPES Bex-2236/09-5Public Library ScienceUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Albert Einstein HospHarvard UnivLarocca, Rafael AssumpcaoMoraes-Vieira, Pedro ManoelBassi, Enio JoseSemedo, Patricia [UNIFESP]Almeida, Danilo Candido de [UNIFESP]Silva, Marina Burgos daThornley, ThomasPacheco-Silva, Alvaro [UNIFESP]Câmara, Niels Olsen Saraiva [UNIFESP]2016-01-24T14:34:34Z2016-01-24T14:34:34Z2013-10-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9http://dx.doi.org/10.1371/journal.pone.0076396Plos One. San Francisco: Public Library Science, v. 8, n. 10, 9 p., 2013.10.1371/journal.pone.0076396WOS000325489100114.pdf1932-6203http://repositorio.unifesp.br/handle/11600/36868WOS:000325489100114engPlos Oneinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T12:34:34Zoai:repositorio.unifesp.br/:11600/36868Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T12:34:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response
title Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response
spellingShingle Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response
Larocca, Rafael Assumpcao
title_short Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response
title_full Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response
title_fullStr Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response
title_full_unstemmed Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response
title_sort Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response
author Larocca, Rafael Assumpcao
author_facet Larocca, Rafael Assumpcao
Moraes-Vieira, Pedro Manoel
Bassi, Enio Jose
Semedo, Patricia [UNIFESP]
Almeida, Danilo Candido de [UNIFESP]
Silva, Marina Burgos da
Thornley, Thomas
Pacheco-Silva, Alvaro [UNIFESP]
Câmara, Niels Olsen Saraiva [UNIFESP]
author_role author
author2 Moraes-Vieira, Pedro Manoel
Bassi, Enio Jose
Semedo, Patricia [UNIFESP]
Almeida, Danilo Candido de [UNIFESP]
Silva, Marina Burgos da
Thornley, Thomas
Pacheco-Silva, Alvaro [UNIFESP]
Câmara, Niels Olsen Saraiva [UNIFESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Albert Einstein Hosp
Harvard Univ
dc.contributor.author.fl_str_mv Larocca, Rafael Assumpcao
Moraes-Vieira, Pedro Manoel
Bassi, Enio Jose
Semedo, Patricia [UNIFESP]
Almeida, Danilo Candido de [UNIFESP]
Silva, Marina Burgos da
Thornley, Thomas
Pacheco-Silva, Alvaro [UNIFESP]
Câmara, Niels Olsen Saraiva [UNIFESP]
description Adipose tissue-derived mesenchymal stem cells (ADSC) exhibit immunosuppressive capabilities both in vitro and in vivo. Their use for therapy in the transplant field is attractive as they could render the use of immunosuppressive drugs unnecessary. the aim of this study was to investigate the effect of ADSC therapy on prolonging skin allograft survival. Animals that were treated with a single injection of donor allogeneic ADSC one day after transplantation showed an increase in donor skin graft survival by approximately one week. This improvement was associated with preserved histological morphology, an expansion of CD4(+) regulatory T cells (Treg) in draining lymph nodes, as well as heightened IL-10 expression and down-regulated IL-17 expression. in vitro, ADSC inhibit naive CD4(+) T cell proliferation and constrain Th-1 and Th-17 polarization. in summary, infusion of ADSC one day post-transplantation dramatically increases skin allograft survival by inhibiting the Th-17 pathogenic immune response and enhancing the protective Treg immune response. Finally, these data suggest that ADSC therapy will open new opportunities for promoting drug-free allograft survival in clinical transplantation.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-04
2016-01-24T14:34:34Z
2016-01-24T14:34:34Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0076396
Plos One. San Francisco: Public Library Science, v. 8, n. 10, 9 p., 2013.
10.1371/journal.pone.0076396
WOS000325489100114.pdf
1932-6203
http://repositorio.unifesp.br/handle/11600/36868
WOS:000325489100114
url http://dx.doi.org/10.1371/journal.pone.0076396
http://repositorio.unifesp.br/handle/11600/36868
identifier_str_mv Plos One. San Francisco: Public Library Science, v. 8, n. 10, 9 p., 2013.
10.1371/journal.pone.0076396
WOS000325489100114.pdf
1932-6203
WOS:000325489100114
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plos One
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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