Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0076396 http://repositorio.unifesp.br/handle/11600/36868 |
Resumo: | Adipose tissue-derived mesenchymal stem cells (ADSC) exhibit immunosuppressive capabilities both in vitro and in vivo. Their use for therapy in the transplant field is attractive as they could render the use of immunosuppressive drugs unnecessary. the aim of this study was to investigate the effect of ADSC therapy on prolonging skin allograft survival. Animals that were treated with a single injection of donor allogeneic ADSC one day after transplantation showed an increase in donor skin graft survival by approximately one week. This improvement was associated with preserved histological morphology, an expansion of CD4(+) regulatory T cells (Treg) in draining lymph nodes, as well as heightened IL-10 expression and down-regulated IL-17 expression. in vitro, ADSC inhibit naive CD4(+) T cell proliferation and constrain Th-1 and Th-17 polarization. in summary, infusion of ADSC one day post-transplantation dramatically increases skin allograft survival by inhibiting the Th-17 pathogenic immune response and enhancing the protective Treg immune response. Finally, these data suggest that ADSC therapy will open new opportunities for promoting drug-free allograft survival in clinical transplantation. |
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Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune ResponseAdipose tissue-derived mesenchymal stem cells (ADSC) exhibit immunosuppressive capabilities both in vitro and in vivo. Their use for therapy in the transplant field is attractive as they could render the use of immunosuppressive drugs unnecessary. the aim of this study was to investigate the effect of ADSC therapy on prolonging skin allograft survival. Animals that were treated with a single injection of donor allogeneic ADSC one day after transplantation showed an increase in donor skin graft survival by approximately one week. This improvement was associated with preserved histological morphology, an expansion of CD4(+) regulatory T cells (Treg) in draining lymph nodes, as well as heightened IL-10 expression and down-regulated IL-17 expression. in vitro, ADSC inhibit naive CD4(+) T cell proliferation and constrain Th-1 and Th-17 polarization. in summary, infusion of ADSC one day post-transplantation dramatically increases skin allograft survival by inhibiting the Th-17 pathogenic immune response and enhancing the protective Treg immune response. Finally, these data suggest that ADSC therapy will open new opportunities for promoting drug-free allograft survival in clinical transplantation.Univ São Paulo, Lab Transplantat Immunobiol, Dept Immunol, Inst Biomed Sci, São Paulo, BrazilUniversidade Federal de São Paulo, Lab Clin & Expt Immunol, Div Nephrol, São Paulo, BrazilAlbert Einstein Hosp, Inst Israelita Ensino & Pesquisa, Renal Transplantat Div, São Paulo, BrazilHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Dept Med,Transplant Inst, Boston, MA 02215 USAHarvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Dept Med,Div Endocrinol, Boston, MA 02215 USAUniversidade Federal de São Paulo, Lab Clin & Expt Immunol, Div Nephrol, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian Council of Scientific and Technologic DevelopmentComplex Fluids INCT, Coordination of Improvement of Higher Education PersonnelFAPESP: 07/07139-3FAPESP: 06/55326-4FAPESP: 10/52180-4FAPESP: 12/02270-2Brazilian Council of Scientific and Technologic Development: 470533/2007-2Brazilian Council of Scientific and Technologic Development: CNPq/DECIT/MSComplex Fluids INCT, Coordination of Improvement of Higher Education Personnel: CAPES Bex-2236/09-5Public Library ScienceUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Albert Einstein HospHarvard UnivLarocca, Rafael AssumpcaoMoraes-Vieira, Pedro ManoelBassi, Enio JoseSemedo, Patricia [UNIFESP]Almeida, Danilo Candido de [UNIFESP]Silva, Marina Burgos daThornley, ThomasPacheco-Silva, Alvaro [UNIFESP]Câmara, Niels Olsen Saraiva [UNIFESP]2016-01-24T14:34:34Z2016-01-24T14:34:34Z2013-10-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion9http://dx.doi.org/10.1371/journal.pone.0076396Plos One. San Francisco: Public Library Science, v. 8, n. 10, 9 p., 2013.10.1371/journal.pone.0076396WOS000325489100114.pdf1932-6203http://repositorio.unifesp.br/handle/11600/36868WOS:000325489100114engPlos Oneinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T12:34:34Zoai:repositorio.unifesp.br/:11600/36868Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T12:34:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response |
title |
Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response |
spellingShingle |
Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response Larocca, Rafael Assumpcao |
title_short |
Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response |
title_full |
Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response |
title_fullStr |
Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response |
title_full_unstemmed |
Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response |
title_sort |
Adipose Tissue-Derived Mesenchymal Stem Cells Increase Skin Allograft Survival and Inhibit Th-17 Immune Response |
author |
Larocca, Rafael Assumpcao |
author_facet |
Larocca, Rafael Assumpcao Moraes-Vieira, Pedro Manoel Bassi, Enio Jose Semedo, Patricia [UNIFESP] Almeida, Danilo Candido de [UNIFESP] Silva, Marina Burgos da Thornley, Thomas Pacheco-Silva, Alvaro [UNIFESP] Câmara, Niels Olsen Saraiva [UNIFESP] |
author_role |
author |
author2 |
Moraes-Vieira, Pedro Manoel Bassi, Enio Jose Semedo, Patricia [UNIFESP] Almeida, Danilo Candido de [UNIFESP] Silva, Marina Burgos da Thornley, Thomas Pacheco-Silva, Alvaro [UNIFESP] Câmara, Niels Olsen Saraiva [UNIFESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) Albert Einstein Hosp Harvard Univ |
dc.contributor.author.fl_str_mv |
Larocca, Rafael Assumpcao Moraes-Vieira, Pedro Manoel Bassi, Enio Jose Semedo, Patricia [UNIFESP] Almeida, Danilo Candido de [UNIFESP] Silva, Marina Burgos da Thornley, Thomas Pacheco-Silva, Alvaro [UNIFESP] Câmara, Niels Olsen Saraiva [UNIFESP] |
description |
Adipose tissue-derived mesenchymal stem cells (ADSC) exhibit immunosuppressive capabilities both in vitro and in vivo. Their use for therapy in the transplant field is attractive as they could render the use of immunosuppressive drugs unnecessary. the aim of this study was to investigate the effect of ADSC therapy on prolonging skin allograft survival. Animals that were treated with a single injection of donor allogeneic ADSC one day after transplantation showed an increase in donor skin graft survival by approximately one week. This improvement was associated with preserved histological morphology, an expansion of CD4(+) regulatory T cells (Treg) in draining lymph nodes, as well as heightened IL-10 expression and down-regulated IL-17 expression. in vitro, ADSC inhibit naive CD4(+) T cell proliferation and constrain Th-1 and Th-17 polarization. in summary, infusion of ADSC one day post-transplantation dramatically increases skin allograft survival by inhibiting the Th-17 pathogenic immune response and enhancing the protective Treg immune response. Finally, these data suggest that ADSC therapy will open new opportunities for promoting drug-free allograft survival in clinical transplantation. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-04 2016-01-24T14:34:34Z 2016-01-24T14:34:34Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0076396 Plos One. San Francisco: Public Library Science, v. 8, n. 10, 9 p., 2013. 10.1371/journal.pone.0076396 WOS000325489100114.pdf 1932-6203 http://repositorio.unifesp.br/handle/11600/36868 WOS:000325489100114 |
url |
http://dx.doi.org/10.1371/journal.pone.0076396 http://repositorio.unifesp.br/handle/11600/36868 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 8, n. 10, 9 p., 2013. 10.1371/journal.pone.0076396 WOS000325489100114.pdf 1932-6203 WOS:000325489100114 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268352713981952 |