Plasma Metabolomics Biosignature According to HIV Stage of Infection, Pace of Disease Progression, Viremia Level and Immunological Response to Treatment

Detalhes bibliográficos
Autor(a) principal: Scarpelini, Bruno [UNIFESP]
Data de Publicação: 2016
Outros Autores: Zanoni, Michelle [UNIFESP], Sucupira, Maria Cecília Araripe [UNIFESP], Truong, Hong-Ha M., Janini, Luiz Mário Ramos [UNIFESP], Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP], Diaz, Ricardo Sobhie [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0161920
https://repositorio.unifesp.br/handle/11600/56550
Resumo: Background We evaluated plasma samples HIV-infected individuals with different phenotypic profile among five HIV-infected elite controllers and five rapid progressors after recent HIV infection and one year later and from 10 individuals subjected to antiretroviral therapy, five of whom were immunological non-responders (INR), before and after one year of antiretroviral treatment compared to 175 samples from HIV-negative patients. A targeted quantitative tandem mass spectrometry metabolomics approach was used in order to determine plasma metabolomics biosignature that may relate to HIV infection, pace of HIV disease progression, and immunological response to treatment. Results Twenty-five unique metabolites were identified, including five metabolites that could distinguish rapid progressors and INRs at baseline. Severe deregulation in acylcarnitine and sphingomyelin metabolism compatible with mitochondrial deficiencies was observed. beta-oxidation and sphingosine-1-phosphate-phosphatase-1 activity were down-regulated, whereas acyl-alkyl-containing phosphatidylcholines and alkylglyceronephosphate synthase levels were elevated in INRs. Evidence that elite controllers harbor an inborn error of metabolism (late-onset multiple acyl-coenzyme A dehydrogenase deficiency [MADD]) was detected. Conclusions Blood-based markers from metabolomics show a very high accuracy of discriminating HIV infection between varieties of controls and have the ability to predict rapid disease progression or poor antiretroviral immunological response. These metabolites can be used as biomarkers of HIV natural evolution or treatment response and provide insight into the mechanisms of the disease.
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spelling Plasma Metabolomics Biosignature According to HIV Stage of Infection, Pace of Disease Progression, Viremia Level and Immunological Response to TreatmentBackground We evaluated plasma samples HIV-infected individuals with different phenotypic profile among five HIV-infected elite controllers and five rapid progressors after recent HIV infection and one year later and from 10 individuals subjected to antiretroviral therapy, five of whom were immunological non-responders (INR), before and after one year of antiretroviral treatment compared to 175 samples from HIV-negative patients. A targeted quantitative tandem mass spectrometry metabolomics approach was used in order to determine plasma metabolomics biosignature that may relate to HIV infection, pace of HIV disease progression, and immunological response to treatment. Results Twenty-five unique metabolites were identified, including five metabolites that could distinguish rapid progressors and INRs at baseline. Severe deregulation in acylcarnitine and sphingomyelin metabolism compatible with mitochondrial deficiencies was observed. beta-oxidation and sphingosine-1-phosphate-phosphatase-1 activity were down-regulated, whereas acyl-alkyl-containing phosphatidylcholines and alkylglyceronephosphate synthase levels were elevated in INRs. Evidence that elite controllers harbor an inborn error of metabolism (late-onset multiple acyl-coenzyme A dehydrogenase deficiency [MADD]) was detected. Conclusions Blood-based markers from metabolomics show a very high accuracy of discriminating HIV infection between varieties of controls and have the ability to predict rapid disease progression or poor antiretroviral immunological response. These metabolites can be used as biomarkers of HIV natural evolution or treatment response and provide insight into the mechanisms of the disease.Univ Fed Sao Paulo, Dept Med, Sao Paulo, SP, BrazilUniv Calif San Francisco, Dept Med, San Francisco, CA USAUniv Fed Sao Paulo, Dept Microbiol, Sao Paulo, SP, BrazilUniversidade Federal de São Paulo (UNIFESP), Department of Medicine, Sao Paulo—SP, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 04/15856-9Public Library Science2020-07-31T12:47:03Z2020-07-31T12:47:03Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion-application/pdfhttp://dx.doi.org/10.1371/journal.pone.0161920Plos One. San Francisco, v. 11, n. 12, p. -, 2016.10.1371/journal.pone.0161920WOS000392745600002.pdf1932-6203https://repositorio.unifesp.br/handle/11600/56550WOS:000392745600002engPlos OneSan Franciscoinfo:eu-repo/semantics/openAccessScarpelini, Bruno [UNIFESP]Zanoni, Michelle [UNIFESP]Sucupira, Maria Cecília Araripe [UNIFESP]Truong, Hong-Ha M.Janini, Luiz Mário Ramos [UNIFESP]Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]Diaz, Ricardo Sobhie [UNIFESP]reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-03T10:13:16Zoai:repositorio.unifesp.br/:11600/56550Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-03T10:13:16Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Plasma Metabolomics Biosignature According to HIV Stage of Infection, Pace of Disease Progression, Viremia Level and Immunological Response to Treatment
title Plasma Metabolomics Biosignature According to HIV Stage of Infection, Pace of Disease Progression, Viremia Level and Immunological Response to Treatment
spellingShingle Plasma Metabolomics Biosignature According to HIV Stage of Infection, Pace of Disease Progression, Viremia Level and Immunological Response to Treatment
Scarpelini, Bruno [UNIFESP]
title_short Plasma Metabolomics Biosignature According to HIV Stage of Infection, Pace of Disease Progression, Viremia Level and Immunological Response to Treatment
title_full Plasma Metabolomics Biosignature According to HIV Stage of Infection, Pace of Disease Progression, Viremia Level and Immunological Response to Treatment
title_fullStr Plasma Metabolomics Biosignature According to HIV Stage of Infection, Pace of Disease Progression, Viremia Level and Immunological Response to Treatment
title_full_unstemmed Plasma Metabolomics Biosignature According to HIV Stage of Infection, Pace of Disease Progression, Viremia Level and Immunological Response to Treatment
title_sort Plasma Metabolomics Biosignature According to HIV Stage of Infection, Pace of Disease Progression, Viremia Level and Immunological Response to Treatment
author Scarpelini, Bruno [UNIFESP]
author_facet Scarpelini, Bruno [UNIFESP]
Zanoni, Michelle [UNIFESP]
Sucupira, Maria Cecília Araripe [UNIFESP]
Truong, Hong-Ha M.
Janini, Luiz Mário Ramos [UNIFESP]
Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
Diaz, Ricardo Sobhie [UNIFESP]
author_role author
author2 Zanoni, Michelle [UNIFESP]
Sucupira, Maria Cecília Araripe [UNIFESP]
Truong, Hong-Ha M.
Janini, Luiz Mário Ramos [UNIFESP]
Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
Diaz, Ricardo Sobhie [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Scarpelini, Bruno [UNIFESP]
Zanoni, Michelle [UNIFESP]
Sucupira, Maria Cecília Araripe [UNIFESP]
Truong, Hong-Ha M.
Janini, Luiz Mário Ramos [UNIFESP]
Silva, Ismael Dale Cotrim Guerreiro da [UNIFESP]
Diaz, Ricardo Sobhie [UNIFESP]
description Background We evaluated plasma samples HIV-infected individuals with different phenotypic profile among five HIV-infected elite controllers and five rapid progressors after recent HIV infection and one year later and from 10 individuals subjected to antiretroviral therapy, five of whom were immunological non-responders (INR), before and after one year of antiretroviral treatment compared to 175 samples from HIV-negative patients. A targeted quantitative tandem mass spectrometry metabolomics approach was used in order to determine plasma metabolomics biosignature that may relate to HIV infection, pace of HIV disease progression, and immunological response to treatment. Results Twenty-five unique metabolites were identified, including five metabolites that could distinguish rapid progressors and INRs at baseline. Severe deregulation in acylcarnitine and sphingomyelin metabolism compatible with mitochondrial deficiencies was observed. beta-oxidation and sphingosine-1-phosphate-phosphatase-1 activity were down-regulated, whereas acyl-alkyl-containing phosphatidylcholines and alkylglyceronephosphate synthase levels were elevated in INRs. Evidence that elite controllers harbor an inborn error of metabolism (late-onset multiple acyl-coenzyme A dehydrogenase deficiency [MADD]) was detected. Conclusions Blood-based markers from metabolomics show a very high accuracy of discriminating HIV infection between varieties of controls and have the ability to predict rapid disease progression or poor antiretroviral immunological response. These metabolites can be used as biomarkers of HIV natural evolution or treatment response and provide insight into the mechanisms of the disease.
publishDate 2016
dc.date.none.fl_str_mv 2016
2020-07-31T12:47:03Z
2020-07-31T12:47:03Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0161920
Plos One. San Francisco, v. 11, n. 12, p. -, 2016.
10.1371/journal.pone.0161920
WOS000392745600002.pdf
1932-6203
https://repositorio.unifesp.br/handle/11600/56550
WOS:000392745600002
url http://dx.doi.org/10.1371/journal.pone.0161920
https://repositorio.unifesp.br/handle/11600/56550
identifier_str_mv Plos One. San Francisco, v. 11, n. 12, p. -, 2016.
10.1371/journal.pone.0161920
WOS000392745600002.pdf
1932-6203
WOS:000392745600002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plos One
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.coverage.none.fl_str_mv San Francisco
dc.publisher.none.fl_str_mv Public Library Science
publisher.none.fl_str_mv Public Library Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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