Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains

Detalhes bibliográficos
Autor(a) principal: Viana,Roberta Oliveira
Data de Publicação: 2019
Outros Autores: Magalhães-Guedes,Karina Teixeira, Dias,Disney Ribeiro, Schwan,Rosane Freitas
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Revista Ceres
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2019000100072
Resumo: ABSTRACT The aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by Maldi-Tof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.
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spelling Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grainsmicroorganismsbacteria’syeastbiological sensorABSTRACT The aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by Maldi-Tof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.Universidade Federal de Viçosa2019-02-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2019000100072Revista Ceres v.66 n.1 2019reponame:Revista Ceresinstname:Universidade Federal de Viçosa (UFV)instacron:UFV10.1590/0034-737x201966010010info:eu-repo/semantics/openAccessViana,Roberta OliveiraMagalhães-Guedes,Karina TeixeiraDias,Disney RibeiroSchwan,Rosane Freitaseng2019-03-07T00:00:00ZRevista
dc.title.none.fl_str_mv Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
title Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
spellingShingle Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
Viana,Roberta Oliveira
microorganisms
bacteria’s
yeast
biological sensor
title_short Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
title_full Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
title_fullStr Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
title_full_unstemmed Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
title_sort Use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains
author Viana,Roberta Oliveira
author_facet Viana,Roberta Oliveira
Magalhães-Guedes,Karina Teixeira
Dias,Disney Ribeiro
Schwan,Rosane Freitas
author_role author
author2 Magalhães-Guedes,Karina Teixeira
Dias,Disney Ribeiro
Schwan,Rosane Freitas
author2_role author
author
author
dc.contributor.author.fl_str_mv Viana,Roberta Oliveira
Magalhães-Guedes,Karina Teixeira
Dias,Disney Ribeiro
Schwan,Rosane Freitas
dc.subject.por.fl_str_mv microorganisms
bacteria’s
yeast
biological sensor
topic microorganisms
bacteria’s
yeast
biological sensor
dc.description.none.fl_txt_mv ABSTRACT The aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by Maldi-Tof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.
description ABSTRACT The aim of this study was to evaluate the use of Maldi-Tof MS biosensor in microbial assessment of Brazilian kefir grains. Maldi-Tof MS is a new methodology for the rapid diagnosis of microorganisms. A total of 358 microorganisms were isolated, 31 were yeasts and 327 were bacteria (divided into lactic and acetic bacteria). Microbial colonies were grown in Luria-Bertani agar medium and incubated at 35 °C for 18h and used in the identification of species by Maldi-Tof MS. The microbial population identified in Brazilian kefir grains was Lactobacillus paracasei, Saccharomyces cerevisiae, Lactobacillus plantarum, Acetobacter pasteurianus, and Acetobacter syzygii. This study demonstrated a rapid and accurate identification of the Brazilian kefir grains microorganisms using the Maldi-Tof MS biosensor. In conclusion, the Maldi-Tof MS technology can facilitate the microbiological control in a fermentation process using kefir grains as starter cultures.
publishDate 2019
dc.date.none.fl_str_mv 2019-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2019000100072
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-737X2019000100072
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0034-737x201966010010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Viçosa
publisher.none.fl_str_mv Universidade Federal de Viçosa
dc.source.none.fl_str_mv Revista Ceres v.66 n.1 2019
reponame:Revista Ceres
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str Revista Ceres
collection Revista Ceres
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1728006783426887680

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