Treatment of soy milk with Debaryomyces hansenii cells immobilised in alginate

• Autor(a) principal: Souza Júnior, Waldeck Campanha de
• Data de Publicação: 2009
• Outros Autores: Rezende, Sebastião Tavares de, Viana, Pollyanna Amaral, Falkoski, Daniel Luciano, Reis, Angélica Pataro, Machado, Solimar Gonçalves, Barros, Everaldo Gonçalves de, Guimarães, Valéria Monteze
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: LOCUS Repositório Institucional da UFV
• Texto Completo: https://doi.org/10.1016/j.foodchem.2008.09.090; http://www.locus.ufv.br/handle/123456789/21828
• topic: Calcium alginate; Cell immobilization; Debaryomyces hansenii UFV-1; α- Galactosidase; Galactooligosaccharides

Whole cells of Debaryomyces hansenii UFV-1 were permeabilised with ethanol and immobilised in calcium alginate hydrogel. The optimum pH and temperature for α-galactosidase activities of permeabilised free (PFC) and permeabilised immobilised cells (PIC) were 4.5 and 60 °C; and 4.0 and 70 °C, respectively. PIC α-galactosidase was more stable than that of PFC. The incubation of PIC at 60 and 70 °C promoted an increase in α-galactosidase activity. The α-galactosidase activity was maintained when PIC was used in three repeated batches. The Km values for PIC and PFC α-galactosidases, with ρNPαGal, were 0.82 mM and 0.30 mM, respectively. Soy milk treatment with PIC for 6 h at 60 °C promoted 100% hydrolysis of raffinose oligosaccharides.